Processing 1 helix: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Score = -0.037079 Processing 1 helix: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Score = 0.078379 Processing 27 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -1801.21 Transmembrane helix 2 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 3 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 4 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 5 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 6 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 7 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 8 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 9 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 10 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 11 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 12 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 13 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 14 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 15 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 16 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 17 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 18 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 19 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 20 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 21 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 22 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 23 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 24 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 25 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 26 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 27 from 1 (in) to 0 (out) : -38.981 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 39 (in) to 54 (out) : -1316.74 Transmembrane helix 3 from 58 (out) to 73 (in) : -1384.89 Transmembrane helix 4 from 79 (in) to 94 (out) : -794.334 Transmembrane helix 5 from 98 (out) to 113 (in) : -659.509 Transmembrane helix 6 from 117 (in) to 132 (out) : -965.048 Transmembrane helix 7 from 136 (out) to 151 (in) : -901.774 Transmembrane helix 8 from 155 (in) to 170 (out) : -811.396 Transmembrane helix 9 from 174 (out) to 189 (in) : -872.129 Transmembrane helix 10 from 193 (in) to 208 (out) : -810.678 Transmembrane helix 11 from 212 (out) to 227 (in) : -850.718 Transmembrane helix 12 from 231 (in) to 246 (out) : -687.231 Transmembrane helix 13 from 250 (out) to 265 (in) : -524.336 Transmembrane helix 14 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 15 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 16 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 17 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 18 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 19 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 20 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 21 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 22 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 23 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 24 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 25 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 26 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 45 (out) to 60 (in) : -1235.69 Transmembrane helix 3 from 79 (in) to 94 (out) : -794.334 Transmembrane helix 4 from 98 (out) to 113 (in) : -659.509 Transmembrane helix 5 from 117 (in) to 132 (out) : -965.048 Transmembrane helix 6 from 136 (out) to 151 (in) : -901.774 Transmembrane helix 7 from 155 (in) to 170 (out) : -811.396 Transmembrane helix 8 from 174 (out) to 189 (in) : -872.129 Transmembrane helix 9 from 193 (in) to 208 (out) : -810.678 Transmembrane helix 10 from 212 (out) to 227 (in) : -850.718 Transmembrane helix 11 from 231 (in) to 246 (out) : -687.231 Transmembrane helix 12 from 250 (out) to 265 (in) : -524.336 Transmembrane helix 13 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 14 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 15 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 16 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 17 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 18 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 19 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 20 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 21 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 22 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 23 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 24 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 25 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 79 (in) to 94 (out) : -794.334 Transmembrane helix 3 from 98 (out) to 113 (in) : -659.509 Transmembrane helix 4 from 117 (in) to 132 (out) : -965.048 Transmembrane helix 5 from 136 (out) to 151 (in) : -901.774 Transmembrane helix 6 from 155 (in) to 170 (out) : -811.396 Transmembrane helix 7 from 174 (out) to 189 (in) : -872.129 Transmembrane helix 8 from 193 (in) to 208 (out) : -810.678 Transmembrane helix 9 from 212 (out) to 227 (in) : -850.718 Transmembrane helix 10 from 231 (in) to 246 (out) : -687.231 Transmembrane helix 11 from 250 (out) to 265 (in) : -524.336 Transmembrane helix 12 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 13 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 14 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 15 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 16 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 17 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 18 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 19 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 20 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 21 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 22 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 23 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 24 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 81 (out) to 96 (in) : -696.87 Transmembrane helix 3 from 100 (in) to 115 (out) : -744.812 Transmembrane helix 4 from 122 (out) to 137 (in) : -932.63 Transmembrane helix 5 from 141 (in) to 156 (out) : -735.087 Transmembrane helix 6 from 160 (out) to 175 (in) : -882.28 Transmembrane helix 7 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 8 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 9 from 229 (in) to 244 (out) : -748.313 Transmembrane helix 10 from 248 (out) to 263 (in) : -452.767 Transmembrane helix 11 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 12 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 13 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 14 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 15 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 16 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 17 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 18 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 19 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 20 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 21 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 22 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 23 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 81 (in) to 96 (out) : -701.43 Transmembrane helix 3 from 100 (out) to 115 (in) : -734.088 Transmembrane helix 4 from 126 (in) to 141 (out) : -925.726 Transmembrane helix 5 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 6 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 7 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 8 from 229 (in) to 244 (out) : -748.313 Transmembrane helix 9 from 248 (out) to 263 (in) : -452.767 Transmembrane helix 10 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 11 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 12 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 13 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 14 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 15 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 16 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 17 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 18 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 19 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 20 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 21 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 22 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 81 (out) to 96 (in) : -696.87 Transmembrane helix 3 from 100 (in) to 115 (out) : -744.812 Transmembrane helix 4 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 5 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 6 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 7 from 229 (in) to 244 (out) : -748.313 Transmembrane helix 8 from 248 (out) to 263 (in) : -452.767 Transmembrane helix 9 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 10 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 11 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 12 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 13 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 14 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 15 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 16 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 17 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 18 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 19 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 20 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 21 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 4 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 5 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 6 from 229 (in) to 244 (out) : -748.313 Transmembrane helix 7 from 248 (out) to 263 (in) : -452.767 Transmembrane helix 8 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 9 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 10 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 11 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 12 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 13 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 14 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 15 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 16 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 17 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 18 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 19 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 20 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 93 (out) to 108 (in) : -567.222 Transmembrane helix 3 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 4 from 182 (out) to 197 (in) : -783.172 Transmembrane helix 5 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 6 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 7 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 8 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 9 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 10 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 11 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 12 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 13 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 14 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 15 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 16 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 17 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 18 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 19 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 4 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 5 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 6 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 7 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 8 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 9 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 10 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 11 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 12 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 13 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 14 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 15 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 16 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 17 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 18 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 93 (out) to 108 (in) : -567.222 Transmembrane helix 3 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 4 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 5 from 269 (in) to 284 (out) : -603.237 Transmembrane helix 6 from 288 (out) to 303 (in) : -145.027 Transmembrane helix 7 from 307 (in) to 322 (out) : -168.472 Transmembrane helix 8 from 326 (out) to 341 (in) : -155.75 Transmembrane helix 9 from 345 (in) to 360 (out) : -379.271 Transmembrane helix 10 from 364 (out) to 379 (in) : -392.884 Transmembrane helix 11 from 383 (in) to 398 (out) : -475.725 Transmembrane helix 12 from 402 (out) to 417 (in) : -299.428 Transmembrane helix 13 from 421 (in) to 436 (out) : -75.904 Transmembrane helix 14 from 440 (out) to 455 (in) : -171.792 Transmembrane helix 15 from 459 (in) to 474 (out) : -160.942 Transmembrane helix 16 from 478 (out) to 493 (in) : -194.944 Transmembrane helix 17 from 497 (in) to 512 (out) : -191.303 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 4 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 5 from 277 (out) to 292 (in) : -304.298 Transmembrane helix 6 from 296 (in) to 311 (out) : -154.383 Transmembrane helix 7 from 315 (out) to 330 (in) : -127.68 Transmembrane helix 8 from 334 (in) to 349 (out) : -255.809 Transmembrane helix 9 from 353 (out) to 368 (in) : -374.475 Transmembrane helix 10 from 372 (in) to 387 (out) : -418.876 Transmembrane helix 11 from 397 (out) to 412 (in) : -361.233 Transmembrane helix 12 from 416 (in) to 431 (out) : -78.103 Transmembrane helix 13 from 435 (out) to 450 (in) : -144.861 Transmembrane helix 14 from 454 (in) to 469 (out) : -144.807 Transmembrane helix 15 from 473 (out) to 488 (in) : -219.412 Transmembrane helix 16 from 492 (in) to 507 (out) : -131.153 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 92 (out) to 107 (in) : -566.571 Transmembrane helix 3 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 4 from 277 (out) to 292 (in) : -304.298 Transmembrane helix 5 from 296 (in) to 311 (out) : -154.383 Transmembrane helix 6 from 315 (out) to 330 (in) : -127.68 Transmembrane helix 7 from 334 (in) to 349 (out) : -255.809 Transmembrane helix 8 from 353 (out) to 368 (in) : -374.475 Transmembrane helix 9 from 372 (in) to 387 (out) : -418.876 Transmembrane helix 10 from 397 (out) to 412 (in) : -361.233 Transmembrane helix 11 from 416 (in) to 431 (out) : -78.103 Transmembrane helix 12 from 435 (out) to 450 (in) : -144.861 Transmembrane helix 13 from 454 (in) to 469 (out) : -144.807 Transmembrane helix 14 from 473 (out) to 488 (in) : -219.412 Transmembrane helix 15 from 492 (in) to 507 (out) : -131.153 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 3 from 277 (out) to 292 (in) : -304.298 Transmembrane helix 4 from 296 (in) to 311 (out) : -154.383 Transmembrane helix 5 from 315 (out) to 330 (in) : -127.68 Transmembrane helix 6 from 334 (in) to 349 (out) : -255.809 Transmembrane helix 7 from 353 (out) to 368 (in) : -374.475 Transmembrane helix 8 from 372 (in) to 387 (out) : -418.876 Transmembrane helix 9 from 397 (out) to 412 (in) : -361.233 Transmembrane helix 10 from 416 (in) to 431 (out) : -78.103 Transmembrane helix 11 from 435 (out) to 450 (in) : -144.861 Transmembrane helix 12 from 454 (in) to 469 (out) : -144.807 Transmembrane helix 13 from 473 (out) to 488 (in) : -219.412 Transmembrane helix 14 from 492 (in) to 507 (out) : -131.153 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 3 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 4 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 5 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 6 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 7 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 8 from 397 (out) to 412 (in) : -361.233 Transmembrane helix 9 from 416 (in) to 431 (out) : -78.103 Transmembrane helix 10 from 435 (out) to 450 (in) : -144.861 Transmembrane helix 11 from 454 (in) to 469 (out) : -144.807 Transmembrane helix 12 from 473 (out) to 488 (in) : -219.412 Transmembrane helix 13 from 492 (in) to 507 (out) : -131.153 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 3 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 4 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 5 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 6 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 7 from 397 (out) to 412 (in) : -361.233 Transmembrane helix 8 from 416 (in) to 431 (out) : -78.103 Transmembrane helix 9 from 435 (out) to 450 (in) : -144.861 Transmembrane helix 10 from 454 (in) to 469 (out) : -144.807 Transmembrane helix 11 from 473 (out) to 488 (in) : -219.412 Transmembrane helix 12 from 492 (in) to 507 (out) : -131.153 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 3 from 285 (in) to 300 (out) : -167.369 Transmembrane helix 4 from 304 (out) to 319 (in) : -163.645 Transmembrane helix 5 from 323 (in) to 338 (out) : -126.264 Transmembrane helix 6 from 342 (out) to 357 (in) : -358.813 Transmembrane helix 7 from 415 (in) to 430 (out) : -86.569 Transmembrane helix 8 from 434 (out) to 449 (in) : -135.531 Transmembrane helix 9 from 453 (in) to 468 (out) : -146.577 Transmembrane helix 10 from 472 (out) to 487 (in) : -219.891 Transmembrane helix 11 from 491 (in) to 506 (out) : -127.573 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 285 (in) to 300 (out) : -167.369 Transmembrane helix 3 from 304 (out) to 319 (in) : -163.645 Transmembrane helix 4 from 323 (in) to 338 (out) : -126.264 Transmembrane helix 5 from 342 (out) to 357 (in) : -358.813 Transmembrane helix 6 from 415 (in) to 430 (out) : -86.569 Transmembrane helix 7 from 434 (out) to 449 (in) : -135.531 Transmembrane helix 8 from 453 (in) to 468 (out) : -146.577 Transmembrane helix 9 from 472 (out) to 487 (in) : -219.891 Transmembrane helix 10 from 491 (in) to 506 (out) : -127.573 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 290 (out) to 305 (in) : -134.258 Transmembrane helix 3 from 309 (in) to 324 (out) : -156.143 Transmembrane helix 4 from 328 (out) to 343 (in) : -173.237 Transmembrane helix 5 from 415 (in) to 430 (out) : -86.569 Transmembrane helix 6 from 434 (out) to 449 (in) : -135.531 Transmembrane helix 7 from 453 (in) to 468 (out) : -146.577 Transmembrane helix 8 from 472 (out) to 487 (in) : -219.891 Transmembrane helix 9 from 491 (in) to 506 (out) : -127.573 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 286 (in) to 301 (out) : -155.747 Transmembrane helix 3 from 305 (out) to 320 (in) : -165.211 Transmembrane helix 4 from 324 (in) to 339 (out) : -132.191 Transmembrane helix 5 from 416 (out) to 431 (in) : -85.397 Transmembrane helix 6 from 435 (in) to 450 (out) : -144.339 Transmembrane helix 7 from 454 (out) to 469 (in) : -144.293 Transmembrane helix 8 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 292 (out) to 307 (in) : -128.707 Transmembrane helix 3 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 4 from 416 (out) to 431 (in) : -85.397 Transmembrane helix 5 from 435 (in) to 450 (out) : -144.339 Transmembrane helix 6 from 454 (out) to 469 (in) : -144.293 Transmembrane helix 7 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 291 (in) to 306 (out) : -130.995 Transmembrane helix 3 from 320 (out) to 335 (in) : -114.299 Transmembrane helix 4 from 415 (in) to 430 (out) : -86.569 Transmembrane helix 5 from 434 (out) to 449 (in) : -135.531 Transmembrane helix 6 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 292 (out) to 307 (in) : -128.707 Transmembrane helix 3 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 4 from 423 (out) to 438 (in) : -72.466 Transmembrane helix 5 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 3 from 423 (out) to 438 (in) : -72.466 Transmembrane helix 4 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 423 (out) to 438 (in) : -72.466 Transmembrane helix 3 from 490 (in) to 505 (out) : -126.876 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 419 (in) to 434 (out) : -72.81 Score = -100000.000000 Processing 27 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -1740.81 Transmembrane helix 2 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 3 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 4 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 5 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 6 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 7 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 8 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 9 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 10 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 11 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 12 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 13 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 14 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 15 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 16 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 17 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 18 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 19 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 20 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 21 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 22 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 23 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 24 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 25 from 1 (out) to 0 (in) : 33.004 Transmembrane helix 26 from 1 (in) to 0 (out) : -38.981 Transmembrane helix 27 from 1 (out) to 0 (in) : 33.004 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 39 (out) to 54 (in) : -1312.46 Transmembrane helix 3 from 58 (in) to 73 (out) : -1378.51 Transmembrane helix 4 from 79 (out) to 94 (in) : -794.866 Transmembrane helix 5 from 98 (in) to 113 (out) : -665.091 Transmembrane helix 6 from 117 (out) to 132 (in) : -965.952 Transmembrane helix 7 from 136 (in) to 151 (out) : -903.526 Transmembrane helix 8 from 155 (out) to 170 (in) : -810.604 Transmembrane helix 9 from 174 (in) to 189 (out) : -860.671 Transmembrane helix 10 from 193 (out) to 208 (in) : -817.622 Transmembrane helix 11 from 212 (in) to 227 (out) : -862.482 Transmembrane helix 12 from 231 (out) to 246 (in) : -702.669 Transmembrane helix 13 from 250 (in) to 265 (out) : -533.064 Transmembrane helix 14 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 15 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 16 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 17 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 18 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 19 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 20 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 21 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 22 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 23 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 24 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 25 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 26 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 46 (in) to 61 (out) : -1226.02 Transmembrane helix 3 from 79 (out) to 94 (in) : -794.866 Transmembrane helix 4 from 98 (in) to 113 (out) : -665.091 Transmembrane helix 5 from 117 (out) to 132 (in) : -965.952 Transmembrane helix 6 from 136 (in) to 151 (out) : -903.526 Transmembrane helix 7 from 155 (out) to 170 (in) : -810.604 Transmembrane helix 8 from 174 (in) to 189 (out) : -860.671 Transmembrane helix 9 from 193 (out) to 208 (in) : -817.622 Transmembrane helix 10 from 212 (in) to 227 (out) : -862.482 Transmembrane helix 11 from 231 (out) to 246 (in) : -702.669 Transmembrane helix 12 from 250 (in) to 265 (out) : -533.064 Transmembrane helix 13 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 14 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 15 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 16 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 17 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 18 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 19 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 20 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 21 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 22 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 23 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 24 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 25 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 45 (out) to 60 (in) : -1235.69 Transmembrane helix 3 from 81 (in) to 96 (out) : -701.43 Transmembrane helix 4 from 100 (out) to 115 (in) : -734.088 Transmembrane helix 5 from 119 (in) to 134 (out) : -935.44 Transmembrane helix 6 from 141 (out) to 156 (in) : -724.013 Transmembrane helix 7 from 160 (in) to 175 (out) : -881.52 Transmembrane helix 8 from 182 (out) to 197 (in) : -783.172 Transmembrane helix 9 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 10 from 229 (out) to 244 (in) : -761.587 Transmembrane helix 11 from 248 (in) to 263 (out) : -459.633 Transmembrane helix 12 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 13 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 14 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 15 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 16 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 17 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 18 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 19 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 20 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 21 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 22 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 23 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 24 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 81 (in) to 96 (out) : -701.43 Transmembrane helix 3 from 100 (out) to 115 (in) : -734.088 Transmembrane helix 4 from 119 (in) to 134 (out) : -935.44 Transmembrane helix 5 from 141 (out) to 156 (in) : -724.013 Transmembrane helix 6 from 160 (in) to 175 (out) : -881.52 Transmembrane helix 7 from 182 (out) to 197 (in) : -783.172 Transmembrane helix 8 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 9 from 229 (out) to 244 (in) : -761.587 Transmembrane helix 10 from 248 (in) to 263 (out) : -459.633 Transmembrane helix 11 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 12 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 13 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 14 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 15 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 16 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 17 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 18 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 19 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 20 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 21 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 22 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 23 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 81 (out) to 96 (in) : -696.87 Transmembrane helix 3 from 100 (in) to 115 (out) : -744.812 Transmembrane helix 4 from 126 (out) to 141 (in) : -920.174 Transmembrane helix 5 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 6 from 182 (out) to 197 (in) : -783.172 Transmembrane helix 7 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 8 from 229 (out) to 244 (in) : -761.587 Transmembrane helix 9 from 248 (in) to 263 (out) : -459.633 Transmembrane helix 10 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 11 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 12 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 13 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 14 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 15 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 16 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 17 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 18 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 19 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 20 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 21 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 22 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 81 (in) to 96 (out) : -701.43 Transmembrane helix 3 from 100 (out) to 115 (in) : -734.088 Transmembrane helix 4 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 5 from 182 (out) to 197 (in) : -783.172 Transmembrane helix 6 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 7 from 229 (out) to 244 (in) : -761.587 Transmembrane helix 8 from 248 (in) to 263 (out) : -459.633 Transmembrane helix 9 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 10 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 11 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 12 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 13 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 14 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 15 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 16 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 17 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 18 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 19 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 20 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 21 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 81 (out) to 96 (in) : -696.87 Transmembrane helix 3 from 100 (in) to 115 (out) : -744.812 Transmembrane helix 4 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 5 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 6 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 7 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 8 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 9 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 10 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 11 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 12 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 13 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 14 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 15 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 16 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 17 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 18 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 19 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 20 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 4 from 182 (in) to 197 (out) : -772.628 Transmembrane helix 5 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 6 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 7 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 8 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 9 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 10 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 11 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 12 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 13 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 14 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 15 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 16 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 17 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 18 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 19 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 93 (out) to 108 (in) : -567.222 Transmembrane helix 3 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 4 from 203 (out) to 218 (in) : -695.407 Transmembrane helix 5 from 242 (in) to 257 (out) : -386.837 Transmembrane helix 6 from 269 (out) to 284 (in) : -597.263 Transmembrane helix 7 from 288 (in) to 303 (out) : -137.173 Transmembrane helix 8 from 307 (out) to 322 (in) : -165.028 Transmembrane helix 9 from 326 (in) to 341 (out) : -159.25 Transmembrane helix 10 from 345 (out) to 360 (in) : -380.029 Transmembrane helix 11 from 364 (in) to 379 (out) : -383.416 Transmembrane helix 12 from 383 (out) to 398 (in) : -466.975 Transmembrane helix 13 from 402 (in) to 417 (out) : -296.472 Transmembrane helix 14 from 421 (out) to 436 (in) : -75.996 Transmembrane helix 15 from 440 (in) to 455 (out) : -169.308 Transmembrane helix 16 from 459 (out) to 474 (in) : -154.858 Transmembrane helix 17 from 478 (in) to 493 (out) : -189.256 Transmembrane helix 18 from 497 (out) to 512 (in) : -187.597 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 146 (out) to 161 (in) : -645.265 Transmembrane helix 4 from 203 (in) to 218 (out) : -693.793 Transmembrane helix 5 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 6 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 7 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 8 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 9 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 10 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 11 from 372 (out) to 387 (in) : -400.124 Transmembrane helix 12 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 13 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 14 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 15 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 16 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 17 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 93 (out) to 108 (in) : -567.222 Transmembrane helix 3 from 146 (in) to 161 (out) : -653.635 Transmembrane helix 4 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 5 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 6 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 7 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 8 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 9 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 10 from 372 (out) to 387 (in) : -400.124 Transmembrane helix 11 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 12 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 13 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 14 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 15 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 16 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 92 (in) to 107 (out) : -558.029 Transmembrane helix 3 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 4 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 5 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 6 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 7 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 8 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 9 from 372 (out) to 387 (in) : -400.124 Transmembrane helix 10 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 11 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 12 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 13 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 14 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 15 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 3 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 4 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 5 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 6 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 7 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 8 from 372 (out) to 387 (in) : -400.124 Transmembrane helix 9 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 10 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 11 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 12 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 13 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 14 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 3 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 4 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 5 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 6 from 353 (in) to 368 (out) : -374.225 Transmembrane helix 7 from 372 (out) to 387 (in) : -400.124 Transmembrane helix 8 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 9 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 10 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 11 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 12 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 13 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 244 (out) to 259 (in) : -381.986 Transmembrane helix 3 from 285 (in) to 300 (out) : -167.369 Transmembrane helix 4 from 304 (out) to 319 (in) : -163.645 Transmembrane helix 5 from 323 (in) to 338 (out) : -126.264 Transmembrane helix 6 from 342 (out) to 357 (in) : -358.813 Transmembrane helix 7 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 8 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 9 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 10 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 11 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 12 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 277 (in) to 292 (out) : -295.402 Transmembrane helix 3 from 296 (out) to 311 (in) : -142.417 Transmembrane helix 4 from 315 (in) to 330 (out) : -123.92 Transmembrane helix 5 from 334 (out) to 349 (in) : -256.391 Transmembrane helix 6 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 7 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 8 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 9 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 10 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 11 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 290 (out) to 305 (in) : -134.258 Transmembrane helix 3 from 309 (in) to 324 (out) : -156.143 Transmembrane helix 4 from 328 (out) to 343 (in) : -173.237 Transmembrane helix 5 from 401 (in) to 416 (out) : -317.224 Transmembrane helix 6 from 420 (out) to 435 (in) : -75.063 Transmembrane helix 7 from 439 (in) to 454 (out) : -163.328 Transmembrane helix 8 from 458 (out) to 473 (in) : -150.973 Transmembrane helix 9 from 477 (in) to 492 (out) : -193.657 Transmembrane helix 10 from 496 (out) to 511 (in) : -171.307 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 286 (in) to 301 (out) : -155.747 Transmembrane helix 3 from 305 (out) to 320 (in) : -165.211 Transmembrane helix 4 from 324 (in) to 339 (out) : -132.191 Transmembrane helix 5 from 416 (out) to 431 (in) : -85.397 Transmembrane helix 6 from 435 (in) to 450 (out) : -144.339 Transmembrane helix 7 from 454 (out) to 469 (in) : -144.293 Transmembrane helix 8 from 473 (in) to 488 (out) : -208.588 Transmembrane helix 9 from 492 (out) to 507 (in) : -134.747 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 292 (out) to 307 (in) : -128.707 Transmembrane helix 3 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 4 from 416 (out) to 431 (in) : -85.397 Transmembrane helix 5 from 435 (in) to 450 (out) : -144.339 Transmembrane helix 6 from 454 (out) to 469 (in) : -144.293 Transmembrane helix 7 from 473 (in) to 488 (out) : -208.588 Transmembrane helix 8 from 492 (out) to 507 (in) : -134.747 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 291 (in) to 306 (out) : -130.995 Transmembrane helix 3 from 320 (out) to 335 (in) : -114.299 Transmembrane helix 4 from 415 (in) to 430 (out) : -86.569 Transmembrane helix 5 from 434 (out) to 449 (in) : -135.531 Transmembrane helix 6 from 455 (in) to 470 (out) : -145.903 Transmembrane helix 7 from 491 (out) to 506 (in) : -132.827 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 292 (out) to 307 (in) : -128.707 Transmembrane helix 3 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 4 from 423 (out) to 438 (in) : -72.466 Transmembrane helix 5 from 455 (in) to 470 (out) : -145.903 Transmembrane helix 6 from 491 (out) to 506 (in) : -132.827 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 3 from 423 (out) to 438 (in) : -72.466 Transmembrane helix 4 from 455 (in) to 470 (out) : -145.903 Transmembrane helix 5 from 491 (out) to 506 (in) : -132.827 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 292 (out) to 307 (in) : -128.707 Transmembrane helix 3 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 4 from 423 (out) to 438 (in) : -72.466 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 22.095 Transmembrane helix 2 from 320 (in) to 335 (out) : -118.401 Transmembrane helix 3 from 423 (out) to 438 (in) : -72.466 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 19.205 Transmembrane helix 2 from 423 (out) to 438 (in) : -72.466 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.037079 1 helix (-) : Score = 0.078379 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 24 helices (+) : Score = -100000 24 helices (-) : Score = -100000 25 helices (+) : Score = -100000 25 helices (-) : Score = -100000 26 helices (+) : Score = -100000 26 helices (-) : Score = -100000 27 helices (+) : Score = -100000 27 helices (-) : Score = -100000 Results: Signal peptide: 1-17 Signal score: 42.448 Topology: 20-35 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.078379