Processing 1 helix: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Score = 0.000000 Processing 1 helix: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Score = 0.000000 Processing 15 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -7.718 Transmembrane helix 2 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 3 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 4 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 5 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 6 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 7 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 8 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 9 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 10 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 11 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 12 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 13 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 14 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 15 from 1 (in) to 0 (out) : 10.467 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -289.731 Transmembrane helix 2 from 32 (in) to 47 (out) : -505.186 Transmembrane helix 3 from 56 (out) to 71 (in) : -497.05 Transmembrane helix 4 from 75 (in) to 90 (out) : -118.5 Transmembrane helix 5 from 95 (out) to 110 (in) : -439.4 Transmembrane helix 6 from 114 (in) to 129 (out) : -365.95 Transmembrane helix 7 from 133 (out) to 148 (in) : 0 Transmembrane helix 8 from 152 (in) to 167 (out) : 0 Transmembrane helix 9 from 171 (out) to 186 (in) : 0 Transmembrane helix 10 from 190 (in) to 205 (out) : 0 Transmembrane helix 11 from 209 (out) to 224 (in) : 0 Transmembrane helix 12 from 228 (in) to 243 (out) : 0 Transmembrane helix 13 from 247 (out) to 262 (in) : 0 Transmembrane helix 14 from 266 (in) to 281 (out) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -277.669 Transmembrane helix 2 from 32 (out) to 47 (in) : -527.414 Transmembrane helix 3 from 56 (in) to 71 (out) : -497.05 Transmembrane helix 4 from 75 (out) to 90 (in) : -118.5 Transmembrane helix 5 from 106 (in) to 121 (out) : -105.15 Transmembrane helix 6 from 132 (out) to 147 (in) : 0 Transmembrane helix 7 from 151 (in) to 166 (out) : 0 Transmembrane helix 8 from 170 (out) to 185 (in) : 0 Transmembrane helix 9 from 189 (in) to 204 (out) : 0 Transmembrane helix 10 from 208 (out) to 223 (in) : 0 Transmembrane helix 11 from 227 (in) to 242 (out) : 0 Transmembrane helix 12 from 246 (out) to 261 (in) : 0 Transmembrane helix 13 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -289.731 Transmembrane helix 2 from 32 (in) to 47 (out) : -505.186 Transmembrane helix 3 from 67 (out) to 82 (in) : -38.7 Transmembrane helix 4 from 106 (in) to 121 (out) : -105.15 Transmembrane helix 5 from 132 (out) to 147 (in) : 0 Transmembrane helix 6 from 151 (in) to 166 (out) : 0 Transmembrane helix 7 from 170 (out) to 185 (in) : 0 Transmembrane helix 8 from 189 (in) to 204 (out) : 0 Transmembrane helix 9 from 208 (out) to 223 (in) : 0 Transmembrane helix 10 from 227 (in) to 242 (out) : 0 Transmembrane helix 11 from 246 (out) to 261 (in) : 0 Transmembrane helix 12 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -277.669 Transmembrane helix 2 from 67 (out) to 82 (in) : -38.7 Transmembrane helix 3 from 106 (in) to 121 (out) : -105.15 Transmembrane helix 4 from 132 (out) to 147 (in) : 0 Transmembrane helix 5 from 151 (in) to 166 (out) : 0 Transmembrane helix 6 from 170 (out) to 185 (in) : 0 Transmembrane helix 7 from 189 (in) to 204 (out) : 0 Transmembrane helix 8 from 208 (out) to 223 (in) : 0 Transmembrane helix 9 from 227 (in) to 242 (out) : 0 Transmembrane helix 10 from 246 (out) to 261 (in) : 0 Transmembrane helix 11 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 67 (out) to 82 (in) : -38.7 Transmembrane helix 2 from 106 (in) to 121 (out) : -105.15 Transmembrane helix 3 from 132 (out) to 147 (in) : 0 Transmembrane helix 4 from 151 (in) to 166 (out) : 0 Transmembrane helix 5 from 170 (out) to 185 (in) : 0 Transmembrane helix 6 from 189 (in) to 204 (out) : 0 Transmembrane helix 7 from 208 (out) to 223 (in) : 0 Transmembrane helix 8 from 227 (in) to 242 (out) : 0 Transmembrane helix 9 from 246 (out) to 261 (in) : 0 Transmembrane helix 10 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 67 (in) to 82 (out) : -38.7 Transmembrane helix 2 from 132 (out) to 147 (in) : 0 Transmembrane helix 3 from 151 (in) to 166 (out) : 0 Transmembrane helix 4 from 170 (out) to 185 (in) : 0 Transmembrane helix 5 from 189 (in) to 204 (out) : 0 Transmembrane helix 6 from 208 (out) to 223 (in) : 0 Transmembrane helix 7 from 227 (in) to 242 (out) : 0 Transmembrane helix 8 from 246 (out) to 261 (in) : 0 Transmembrane helix 9 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Transmembrane helix 4 from 189 (in) to 204 (out) : 0 Transmembrane helix 5 from 208 (out) to 223 (in) : 0 Transmembrane helix 6 from 227 (in) to 242 (out) : 0 Transmembrane helix 7 from 246 (out) to 261 (in) : 0 Transmembrane helix 8 from 265 (in) to 280 (out) : 0 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Transmembrane helix 4 from 189 (out) to 204 (in) : 0 Transmembrane helix 5 from 208 (in) to 223 (out) : 0 Transmembrane helix 6 from 227 (out) to 242 (in) : 0 Transmembrane helix 7 from 246 (in) to 261 (out) : 0 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Transmembrane helix 4 from 189 (in) to 204 (out) : 0 Transmembrane helix 5 from 208 (out) to 223 (in) : 0 Transmembrane helix 6 from 227 (in) to 242 (out) : 0 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Transmembrane helix 4 from 189 (out) to 204 (in) : 0 Transmembrane helix 5 from 208 (in) to 223 (out) : 0 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Transmembrane helix 4 from 189 (in) to 204 (out) : 0 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -21.082 Transmembrane helix 2 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 3 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 4 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 5 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 6 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 7 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 8 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 9 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 10 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 11 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 12 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 13 from 1 (out) to 0 (in) : -6.267 Transmembrane helix 14 from 1 (in) to 0 (out) : 10.467 Transmembrane helix 15 from 1 (out) to 0 (in) : -6.267 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -277.669 Transmembrane helix 2 from 32 (out) to 47 (in) : -527.414 Transmembrane helix 3 from 56 (in) to 71 (out) : -497.05 Transmembrane helix 4 from 75 (out) to 90 (in) : -118.5 Transmembrane helix 5 from 95 (in) to 110 (out) : -439.4 Transmembrane helix 6 from 114 (out) to 129 (in) : -365.95 Transmembrane helix 7 from 133 (in) to 148 (out) : 0 Transmembrane helix 8 from 152 (out) to 167 (in) : 0 Transmembrane helix 9 from 171 (in) to 186 (out) : 0 Transmembrane helix 10 from 190 (out) to 205 (in) : 0 Transmembrane helix 11 from 209 (in) to 224 (out) : 0 Transmembrane helix 12 from 228 (out) to 243 (in) : 0 Transmembrane helix 13 from 247 (in) to 262 (out) : 0 Transmembrane helix 14 from 266 (out) to 281 (in) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -289.731 Transmembrane helix 2 from 32 (in) to 47 (out) : -505.186 Transmembrane helix 3 from 56 (out) to 71 (in) : -497.05 Transmembrane helix 4 from 75 (in) to 90 (out) : -118.5 Transmembrane helix 5 from 106 (out) to 121 (in) : -105.15 Transmembrane helix 6 from 132 (in) to 147 (out) : 0 Transmembrane helix 7 from 151 (out) to 166 (in) : 0 Transmembrane helix 8 from 170 (in) to 185 (out) : 0 Transmembrane helix 9 from 189 (out) to 204 (in) : 0 Transmembrane helix 10 from 208 (in) to 223 (out) : 0 Transmembrane helix 11 from 227 (out) to 242 (in) : 0 Transmembrane helix 12 from 246 (in) to 261 (out) : 0 Transmembrane helix 13 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -277.669 Transmembrane helix 2 from 32 (out) to 47 (in) : -527.414 Transmembrane helix 3 from 67 (in) to 82 (out) : -38.7 Transmembrane helix 4 from 106 (out) to 121 (in) : -105.15 Transmembrane helix 5 from 132 (in) to 147 (out) : 0 Transmembrane helix 6 from 151 (out) to 166 (in) : 0 Transmembrane helix 7 from 170 (in) to 185 (out) : 0 Transmembrane helix 8 from 189 (out) to 204 (in) : 0 Transmembrane helix 9 from 208 (in) to 223 (out) : 0 Transmembrane helix 10 from 227 (out) to 242 (in) : 0 Transmembrane helix 11 from 246 (in) to 261 (out) : 0 Transmembrane helix 12 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -289.731 Transmembrane helix 2 from 67 (in) to 82 (out) : -38.7 Transmembrane helix 3 from 106 (out) to 121 (in) : -105.15 Transmembrane helix 4 from 132 (in) to 147 (out) : 0 Transmembrane helix 5 from 151 (out) to 166 (in) : 0 Transmembrane helix 6 from 170 (in) to 185 (out) : 0 Transmembrane helix 7 from 189 (out) to 204 (in) : 0 Transmembrane helix 8 from 208 (in) to 223 (out) : 0 Transmembrane helix 9 from 227 (out) to 242 (in) : 0 Transmembrane helix 10 from 246 (in) to 261 (out) : 0 Transmembrane helix 11 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 67 (in) to 82 (out) : -38.7 Transmembrane helix 2 from 106 (out) to 121 (in) : -105.15 Transmembrane helix 3 from 132 (in) to 147 (out) : 0 Transmembrane helix 4 from 151 (out) to 166 (in) : 0 Transmembrane helix 5 from 170 (in) to 185 (out) : 0 Transmembrane helix 6 from 189 (out) to 204 (in) : 0 Transmembrane helix 7 from 208 (in) to 223 (out) : 0 Transmembrane helix 8 from 227 (out) to 242 (in) : 0 Transmembrane helix 9 from 246 (in) to 261 (out) : 0 Transmembrane helix 10 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 67 (out) to 82 (in) : -38.7 Transmembrane helix 2 from 132 (in) to 147 (out) : 0 Transmembrane helix 3 from 151 (out) to 166 (in) : 0 Transmembrane helix 4 from 170 (in) to 185 (out) : 0 Transmembrane helix 5 from 189 (out) to 204 (in) : 0 Transmembrane helix 6 from 208 (in) to 223 (out) : 0 Transmembrane helix 7 from 227 (out) to 242 (in) : 0 Transmembrane helix 8 from 246 (in) to 261 (out) : 0 Transmembrane helix 9 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Transmembrane helix 4 from 189 (out) to 204 (in) : 0 Transmembrane helix 5 from 208 (in) to 223 (out) : 0 Transmembrane helix 6 from 227 (out) to 242 (in) : 0 Transmembrane helix 7 from 246 (in) to 261 (out) : 0 Transmembrane helix 8 from 265 (out) to 280 (in) : 0 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Transmembrane helix 4 from 189 (in) to 204 (out) : 0 Transmembrane helix 5 from 208 (out) to 223 (in) : 0 Transmembrane helix 6 from 227 (in) to 242 (out) : 0 Transmembrane helix 7 from 246 (out) to 261 (in) : 0 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Transmembrane helix 4 from 189 (out) to 204 (in) : 0 Transmembrane helix 5 from 208 (in) to 223 (out) : 0 Transmembrane helix 6 from 227 (out) to 242 (in) : 0 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Transmembrane helix 4 from 189 (in) to 204 (out) : 0 Transmembrane helix 5 from 208 (out) to 223 (in) : 0 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Transmembrane helix 3 from 170 (in) to 185 (out) : 0 Transmembrane helix 4 from 189 (out) to 204 (in) : 0 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 132 (out) to 147 (in) : 0 Transmembrane helix 2 from 151 (in) to 166 (out) : 0 Transmembrane helix 3 from 170 (out) to 185 (in) : 0 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 132 (in) to 147 (out) : 0 Transmembrane helix 2 from 151 (out) to 166 (in) : 0 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0 1 helix (-) : Score = 0 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 2.037 Topology: 132-147 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: 0