BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (33 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_C1P5Z8 Uncharacterized membrane protein ykgR n=5 Tax=Es...    66   4e-10

>UniRef50_C1P5Z8 Uncharacterized membrane protein ykgR n=5 Tax=Escherichia coli
          RepID=YKGR_ECOLI
          Length = 33

 Score = 65.9 bits (159), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 33/33 (100%), Positives = 33/33 (100%)

Query: 1  MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY 33
          MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY
Sbjct: 1  MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY 33


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

Sequences not found previously or not previously below threshold:

UniRef50_C1P5Z8 Uncharacterized membrane protein ykgR n=5 Tax=Es...    39   0.059


CONVERGED!
>UniRef50_C1P5Z8 Uncharacterized membrane protein ykgR n=5 Tax=Escherichia coli
          RepID=YKGR_ECOLI
          Length = 33

 Score = 38.8 bits (89), Expect = 0.059,   Method: Composition-based stats.
 Identities = 33/33 (100%), Positives = 33/33 (100%)

Query: 1  MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY 33
          MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY
Sbjct: 1  MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY 33


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.335    0.145    0.412 

Lambda     K      H
   0.267   0.0474    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 146,559,944
Number of Sequences: 3077464
Number of extensions: 3150486
Number of successful extensions: 15584
Number of sequences better than 1.0e-01: 1
Number of HSP's better than  0.1 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15582
Number of HSP's gapped (non-prelim): 2
length of query: 33
length of database: 1,040,396,356
effective HSP length: 8
effective length of query: 25
effective length of database: 1,015,776,644
effective search space: 25394416100
effective search space used: 25394416100
T: 11
A: 40
X1: 16 ( 7.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 87 (37.9 bits)