Processing 1 helix: Transmembrane helix 1 from 106 (in) to 133 (out) : 3910.94 Score = 3.910940 Processing 1 helix: Transmembrane helix 1 from 106 (out) to 136 (in) : 3907.62 Score = 3.907622 Processing 21 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 1272.46 Transmembrane helix 2 from 23 (out) to 38 (in) : -361.143 Transmembrane helix 3 from 44 (in) to 59 (out) : 729.762 Transmembrane helix 4 from 63 (out) to 78 (in) : 1476.53 Transmembrane helix 5 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 6 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 7 from 122 (in) to 137 (out) : 2339.35 Transmembrane helix 8 from 141 (out) to 156 (in) : -14.721 Transmembrane helix 9 from 160 (in) to 175 (out) : -18.947 Transmembrane helix 10 from 179 (out) to 194 (in) : -6.477 Transmembrane helix 11 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 12 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 13 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 14 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 15 from 275 (in) to 290 (out) : 0 Transmembrane helix 16 from 294 (out) to 309 (in) : 0 Transmembrane helix 17 from 313 (in) to 328 (out) : 0 Transmembrane helix 18 from 332 (out) to 347 (in) : 0 Transmembrane helix 19 from 351 (in) to 366 (out) : 0 Transmembrane helix 20 from 370 (out) to 385 (in) : 0 Transmembrane helix 21 from 389 (in) to 404 (out) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 44 (in) to 59 (out) : 729.762 Transmembrane helix 3 from 63 (out) to 78 (in) : 1476.53 Transmembrane helix 4 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 5 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 6 from 122 (in) to 137 (out) : 2339.35 Transmembrane helix 7 from 141 (out) to 156 (in) : -14.721 Transmembrane helix 8 from 160 (in) to 175 (out) : -18.947 Transmembrane helix 9 from 179 (out) to 194 (in) : -6.477 Transmembrane helix 10 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 11 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 12 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 13 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 14 from 275 (in) to 290 (out) : 0 Transmembrane helix 15 from 294 (out) to 309 (in) : 0 Transmembrane helix 16 from 313 (in) to 328 (out) : 0 Transmembrane helix 17 from 332 (out) to 347 (in) : 0 Transmembrane helix 18 from 351 (in) to 366 (out) : 0 Transmembrane helix 19 from 370 (out) to 385 (in) : 0 Transmembrane helix 20 from 389 (in) to 404 (out) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 5 from 122 (in) to 137 (out) : 2339.35 Transmembrane helix 6 from 141 (out) to 156 (in) : -14.721 Transmembrane helix 7 from 160 (in) to 175 (out) : -18.947 Transmembrane helix 8 from 179 (out) to 194 (in) : -6.477 Transmembrane helix 9 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 10 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 11 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 12 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 13 from 275 (in) to 290 (out) : 0 Transmembrane helix 14 from 294 (out) to 309 (in) : 0 Transmembrane helix 15 from 313 (in) to 328 (out) : 0 Transmembrane helix 16 from 332 (out) to 347 (in) : 0 Transmembrane helix 17 from 351 (in) to 366 (out) : 0 Transmembrane helix 18 from 370 (out) to 385 (in) : 0 Transmembrane helix 19 from 389 (in) to 404 (out) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 137 (out) to 152 (in) : -15.142 Transmembrane helix 6 from 156 (in) to 171 (out) : -11.233 Transmembrane helix 7 from 175 (out) to 190 (in) : 0.766 Transmembrane helix 8 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 9 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 10 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 11 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 12 from 275 (in) to 290 (out) : 0 Transmembrane helix 13 from 294 (out) to 309 (in) : 0 Transmembrane helix 14 from 313 (in) to 328 (out) : 0 Transmembrane helix 15 from 332 (out) to 347 (in) : 0 Transmembrane helix 16 from 351 (in) to 366 (out) : 0 Transmembrane helix 17 from 370 (out) to 385 (in) : 0 Transmembrane helix 18 from 389 (in) to 404 (out) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 7 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 8 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 9 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 10 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 11 from 275 (in) to 290 (out) : 0 Transmembrane helix 12 from 294 (out) to 309 (in) : 0 Transmembrane helix 13 from 313 (in) to 328 (out) : 0 Transmembrane helix 14 from 332 (out) to 347 (in) : 0 Transmembrane helix 15 from 351 (in) to 366 (out) : 0 Transmembrane helix 16 from 370 (out) to 385 (in) : 0 Transmembrane helix 17 from 389 (in) to 404 (out) : 0 Re-entrant helix 1 from 183 (in) to 195 (in) : 910 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 6 from 199 (in) to 214 (out) : 2.52 Transmembrane helix 7 from 218 (out) to 233 (in) : -0.394 Transmembrane helix 8 from 237 (in) to 252 (out) : -5.195 Transmembrane helix 9 from 256 (out) to 271 (in) : 1.145 Transmembrane helix 10 from 275 (in) to 290 (out) : 0 Transmembrane helix 11 from 294 (out) to 309 (in) : 0 Transmembrane helix 12 from 313 (in) to 328 (out) : 0 Transmembrane helix 13 from 332 (out) to 347 (in) : 0 Transmembrane helix 14 from 351 (in) to 366 (out) : 0 Transmembrane helix 15 from 370 (out) to 385 (in) : 0 Transmembrane helix 16 from 389 (in) to 404 (out) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 183 (in) to 195 (in) : 910 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 7 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 8 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 9 from 248 (in) to 263 (out) : 8.572 Transmembrane helix 10 from 267 (out) to 282 (in) : 0 Transmembrane helix 11 from 286 (in) to 301 (out) : 0 Transmembrane helix 12 from 305 (out) to 320 (in) : 0 Transmembrane helix 13 from 324 (in) to 339 (out) : 0 Transmembrane helix 14 from 343 (out) to 358 (in) : 0 Transmembrane helix 15 from 362 (in) to 377 (out) : 0 Re-entrant helix 1 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 6 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 7 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 8 from 248 (in) to 263 (out) : 8.572 Transmembrane helix 9 from 267 (out) to 282 (in) : 0 Transmembrane helix 10 from 286 (in) to 301 (out) : 0 Transmembrane helix 11 from 305 (out) to 320 (in) : 0 Transmembrane helix 12 from 324 (in) to 339 (out) : 0 Transmembrane helix 13 from 343 (out) to 358 (in) : 0 Transmembrane helix 14 from 362 (in) to 377 (out) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Transmembrane helix 10 from 305 (out) to 320 (in) : 0 Transmembrane helix 11 from 324 (in) to 339 (out) : 0 Transmembrane helix 12 from 343 (out) to 358 (in) : 0 Transmembrane helix 13 from 362 (in) to 377 (out) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Transmembrane helix 9 from 305 (out) to 320 (in) : 0 Transmembrane helix 10 from 324 (in) to 339 (out) : 0 Transmembrane helix 11 from 343 (out) to 358 (in) : 0 Transmembrane helix 12 from 362 (in) to 377 (out) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Transmembrane helix 10 from 305 (out) to 320 (in) : 0 Transmembrane helix 11 from 324 (in) to 339 (out) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Transmembrane helix 9 from 305 (out) to 320 (in) : 0 Transmembrane helix 10 from 324 (in) to 339 (out) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 163 (out) : 27.097 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Score = 10.045128 Processing 3 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 61 (out) to 91 (in) : 3110.58 Transmembrane helix 3 from 106 (in) to 133 (out) : 3910.94 Score = 8.555220 Processing 2 helices: Transmembrane helix 1 from 61 (out) to 91 (in) : 3110.58 Transmembrane helix 2 from 106 (in) to 133 (out) : 3910.94 Score = 7.021029 Processing 21 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 1290.34 Transmembrane helix 2 from 23 (in) to 38 (out) : -354.257 Transmembrane helix 3 from 42 (out) to 57 (in) : 406.578 Transmembrane helix 4 from 61 (in) to 76 (out) : 1811.76 Transmembrane helix 5 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 6 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 7 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 8 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 9 from 161 (out) to 176 (in) : 20.417 Transmembrane helix 10 from 180 (in) to 195 (out) : 7.277 Transmembrane helix 11 from 199 (out) to 214 (in) : -2.52 Transmembrane helix 12 from 218 (in) to 233 (out) : 0.394 Transmembrane helix 13 from 237 (out) to 252 (in) : 5.195 Transmembrane helix 14 from 256 (in) to 271 (out) : -1.145 Transmembrane helix 15 from 275 (out) to 290 (in) : 0 Transmembrane helix 16 from 294 (in) to 309 (out) : 0 Transmembrane helix 17 from 313 (out) to 328 (in) : 0 Transmembrane helix 18 from 332 (in) to 347 (out) : 0 Transmembrane helix 19 from 351 (out) to 366 (in) : 0 Transmembrane helix 20 from 370 (in) to 385 (out) : 0 Transmembrane helix 21 from 389 (out) to 404 (in) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 42 (out) to 57 (in) : 406.578 Transmembrane helix 3 from 61 (in) to 76 (out) : 1811.76 Transmembrane helix 4 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 5 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 6 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 7 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 8 from 161 (out) to 176 (in) : 20.417 Transmembrane helix 9 from 180 (in) to 195 (out) : 7.277 Transmembrane helix 10 from 199 (out) to 214 (in) : -2.52 Transmembrane helix 11 from 218 (in) to 233 (out) : 0.394 Transmembrane helix 12 from 237 (out) to 252 (in) : 5.195 Transmembrane helix 13 from 256 (in) to 271 (out) : -1.145 Transmembrane helix 14 from 275 (out) to 290 (in) : 0 Transmembrane helix 15 from 294 (in) to 309 (out) : 0 Transmembrane helix 16 from 313 (out) to 328 (in) : 0 Transmembrane helix 17 from 332 (in) to 347 (out) : 0 Transmembrane helix 18 from 351 (out) to 366 (in) : 0 Transmembrane helix 19 from 370 (in) to 385 (out) : 0 Transmembrane helix 20 from 389 (out) to 404 (in) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 5 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 6 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 7 from 161 (out) to 176 (in) : 20.417 Transmembrane helix 8 from 180 (in) to 195 (out) : 7.277 Transmembrane helix 9 from 199 (out) to 214 (in) : -2.52 Transmembrane helix 10 from 218 (in) to 233 (out) : 0.394 Transmembrane helix 11 from 237 (out) to 252 (in) : 5.195 Transmembrane helix 12 from 256 (in) to 271 (out) : -1.145 Transmembrane helix 13 from 275 (out) to 290 (in) : 0 Transmembrane helix 14 from 294 (in) to 309 (out) : 0 Transmembrane helix 15 from 313 (out) to 328 (in) : 0 Transmembrane helix 16 from 332 (in) to 347 (out) : 0 Transmembrane helix 17 from 351 (out) to 366 (in) : 0 Transmembrane helix 18 from 370 (in) to 385 (out) : 0 Transmembrane helix 19 from 389 (out) to 404 (in) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 161 (out) to 176 (in) : 20.417 Transmembrane helix 7 from 180 (in) to 195 (out) : 7.277 Transmembrane helix 8 from 199 (out) to 214 (in) : -2.52 Transmembrane helix 9 from 218 (in) to 233 (out) : 0.394 Transmembrane helix 10 from 237 (out) to 252 (in) : 5.195 Transmembrane helix 11 from 256 (in) to 271 (out) : -1.145 Transmembrane helix 12 from 275 (out) to 290 (in) : 0 Transmembrane helix 13 from 294 (in) to 309 (out) : 0 Transmembrane helix 14 from 313 (out) to 328 (in) : 0 Transmembrane helix 15 from 332 (in) to 347 (out) : 0 Transmembrane helix 16 from 351 (out) to 366 (in) : 0 Transmembrane helix 17 from 370 (in) to 385 (out) : 0 Transmembrane helix 18 from 389 (out) to 404 (in) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 161 (out) to 176 (in) : 20.417 Transmembrane helix 6 from 180 (in) to 195 (out) : 7.277 Transmembrane helix 7 from 199 (out) to 214 (in) : -2.52 Transmembrane helix 8 from 218 (in) to 233 (out) : 0.394 Transmembrane helix 9 from 237 (out) to 252 (in) : 5.195 Transmembrane helix 10 from 256 (in) to 271 (out) : -1.145 Transmembrane helix 11 from 275 (out) to 290 (in) : 0 Transmembrane helix 12 from 294 (in) to 309 (out) : 0 Transmembrane helix 13 from 313 (out) to 328 (in) : 0 Transmembrane helix 14 from 332 (in) to 347 (out) : 0 Transmembrane helix 15 from 351 (out) to 366 (in) : 0 Transmembrane helix 16 from 370 (in) to 385 (out) : 0 Transmembrane helix 17 from 389 (out) to 404 (in) : 0 Re-entrant helix 1 from 137 (out) to 157 (out) : 1470 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 7 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 8 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 9 from 248 (in) to 263 (out) : 8.572 Transmembrane helix 10 from 267 (out) to 282 (in) : 0 Transmembrane helix 11 from 286 (in) to 301 (out) : 0 Transmembrane helix 12 from 305 (out) to 320 (in) : 0 Transmembrane helix 13 from 324 (in) to 339 (out) : 0 Transmembrane helix 14 from 343 (out) to 358 (in) : 0 Transmembrane helix 15 from 362 (in) to 377 (out) : 0 Transmembrane helix 16 from 381 (out) to 396 (in) : 0 Re-entrant helix 1 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 6 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 7 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 8 from 248 (in) to 263 (out) : 8.572 Transmembrane helix 9 from 267 (out) to 282 (in) : 0 Transmembrane helix 10 from 286 (in) to 301 (out) : 0 Transmembrane helix 11 from 305 (out) to 320 (in) : 0 Transmembrane helix 12 from 324 (in) to 339 (out) : 0 Transmembrane helix 13 from 343 (out) to 358 (in) : 0 Transmembrane helix 14 from 362 (in) to 377 (out) : 0 Transmembrane helix 15 from 381 (out) to 396 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Transmembrane helix 10 from 305 (out) to 320 (in) : 0 Transmembrane helix 11 from 324 (in) to 339 (out) : 0 Transmembrane helix 12 from 343 (out) to 358 (in) : 0 Transmembrane helix 13 from 362 (in) to 377 (out) : 0 Transmembrane helix 14 from 381 (out) to 396 (in) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Transmembrane helix 9 from 305 (out) to 320 (in) : 0 Transmembrane helix 10 from 324 (in) to 339 (out) : 0 Transmembrane helix 11 from 343 (out) to 358 (in) : 0 Transmembrane helix 12 from 362 (in) to 377 (out) : 0 Transmembrane helix 13 from 381 (out) to 396 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Transmembrane helix 10 from 305 (out) to 320 (in) : 0 Transmembrane helix 11 from 324 (in) to 339 (out) : 0 Transmembrane helix 12 from 343 (out) to 358 (in) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Transmembrane helix 9 from 305 (out) to 320 (in) : 0 Transmembrane helix 10 from 324 (in) to 339 (out) : 0 Transmembrane helix 11 from 343 (out) to 358 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Transmembrane helix 9 from 286 (in) to 301 (out) : 0 Transmembrane helix 10 from 305 (out) to 320 (in) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Transmembrane helix 8 from 286 (in) to 301 (out) : 0 Transmembrane helix 9 from 305 (out) to 320 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 8 from 267 (out) to 282 (in) : 0 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Transmembrane helix 7 from 267 (out) to 282 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 187 (in) : 26.723 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 187 (in) : 26.723 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Score = 9.896234 Processing 3 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 61 (in) to 91 (out) : 3132.32 Transmembrane helix 3 from 106 (out) to 136 (in) : 3907.62 Score = 8.652244 Processing 2 helices: Transmembrane helix 1 from 61 (in) to 91 (out) : 3132.32 Transmembrane helix 2 from 106 (out) to 136 (in) : 3907.62 Score = 7.040433 Summary of topology analysis: 1 helix (+) : Score = 3.91094 1 helix (-) : Score = 3.90762 2 helices (+) : Score = 7.04043 2 helices (-) : Score = 7.02103 3 helices (+) : Score = 8.55522 3 helices (-) : Score = 8.65224 4 helices (+) : Score = 9.89623 4 helices (-) : Score = 10.0451 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.249 Topology: 4-25,50-75,82-99,106-133 Re-entrant helices: Not detected. Helix count: 4 N-terminal: out Score: 10.0451