BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (35 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q6BF25 Small toxic polypeptide ldrD n=124 Tax=Enterobac...    73   3e-12
UniRef50_B7LTE2 Small toxic polypeptide ldrD (Modular protein) n...    72   7e-12
UniRef50_D2TJW8 Small toxic polypeptide ldrD n=2 Tax=Enterobacte...    39   0.036

>UniRef50_Q6BF25 Small toxic polypeptide ldrD n=124 Tax=Enterobacteriaceae
          RepID=LDRD_ECOLI
          Length = 35

 Score = 73.2 bits (178), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 35/35 (100%), Positives = 35/35 (100%)

Query: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35
          MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK
Sbjct: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35


>UniRef50_B7LTE2 Small toxic polypeptide ldrD (Modular protein) n=10
          Tax=Enterobacteriaceae RepID=B7LTE2_ESCF3
          Length = 83

 Score = 71.6 bits (174), Expect = 7e-12,   Method: Compositional matrix adjust.
 Identities = 34/35 (97%), Positives = 34/35 (97%)

Query: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35
          MT AELGMAFWHDLAAPVIAGILASMIVNWLNKRK
Sbjct: 49 MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 83


>UniRef50_D2TJW8 Small toxic polypeptide ldrD n=2 Tax=Enterobacteriaceae
          RepID=D2TJW8_CITRO
          Length = 35

 Score = 39.3 bits (90), Expect = 0.036,   Method: Compositional matrix adjust.
 Identities = 25/35 (71%), Positives = 29/35 (82%)

Query: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35
          MT  +LG+AFWHDLAAP IAGI+A M+VNWL  RK
Sbjct: 1  MTLIQLGIAFWHDLAAPAIAGIIAGMVVNWLRSRK 35


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_B7LTE2 Small toxic polypeptide ldrD (Modular protein) n...    65   7e-10
UniRef50_Q6BF25 Small toxic polypeptide ldrD n=124 Tax=Enterobac...    55   1e-06

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_B7LTE2 Small toxic polypeptide ldrD (Modular protein) n=10
          Tax=Enterobacteriaceae RepID=B7LTE2_ESCF3
          Length = 83

 Score = 65.0 bits (157), Expect = 7e-10,   Method: Composition-based stats.
 Identities = 34/35 (97%), Positives = 34/35 (97%)

Query: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35
          MT AELGMAFWHDLAAPVIAGILASMIVNWLNKRK
Sbjct: 49 MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 83


>UniRef50_Q6BF25 Small toxic polypeptide ldrD n=124 Tax=Enterobacteriaceae
          RepID=LDRD_ECOLI
          Length = 35

 Score = 54.6 bits (130), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 35/35 (100%), Positives = 35/35 (100%)

Query: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35
          MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK
Sbjct: 1  MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK 35


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.331    0.147    0.506 

Lambda     K      H
   0.267   0.0451    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 151,557,768
Number of Sequences: 3077464
Number of extensions: 2965798
Number of successful extensions: 14176
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 14170
Number of HSP's gapped (non-prelim): 6
length of query: 35
length of database: 1,040,396,356
effective HSP length: 9
effective length of query: 26
effective length of database: 1,012,699,180
effective search space: 26330178680
effective search space used: 26330178680
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 87 (38.0 bits)