Processing 1 helix: Transmembrane helix 1 from 96 (in) to 116 (out) : 3409.16 Score = 3.463514 Processing 1 helix: Transmembrane helix 1 from 96 (out) to 116 (in) : 3379.44 Score = 3.325085 Processing 8 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -3740.62 Transmembrane helix 2 from 26 (out) to 41 (in) : 48.335 Transmembrane helix 3 from 45 (in) to 60 (out) : 461.305 Transmembrane helix 4 from 64 (out) to 79 (in) : 1817.3 Transmembrane helix 5 from 83 (in) to 98 (out) : -244.448 Transmembrane helix 6 from 102 (out) to 117 (in) : 2702.8 Transmembrane helix 7 from 121 (in) to 136 (out) : -844.964 Transmembrane helix 8 from 140 (out) to 155 (in) : -1219.56 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 1213.2 Transmembrane helix 2 from 35 (in) to 50 (out) : -291.596 Transmembrane helix 3 from 54 (out) to 69 (in) : 1591.38 Transmembrane helix 4 from 73 (in) to 88 (out) : 270.525 Transmembrane helix 5 from 95 (out) to 110 (in) : 2791.7 Transmembrane helix 6 from 114 (in) to 129 (out) : -183.965 Transmembrane helix 7 from 133 (out) to 148 (in) : -1032.75 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 1220.8 Transmembrane helix 2 from 35 (out) to 50 (in) : -287.804 Transmembrane helix 3 from 54 (in) to 69 (out) : 1602.02 Transmembrane helix 4 from 73 (out) to 88 (in) : 274.275 Transmembrane helix 5 from 95 (in) to 110 (out) : 2806.6 Transmembrane helix 6 from 114 (out) to 129 (in) : -178.035 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 1213.2 Transmembrane helix 2 from 35 (in) to 50 (out) : -291.596 Transmembrane helix 3 from 54 (out) to 69 (in) : 1591.38 Transmembrane helix 4 from 73 (in) to 88 (out) : 270.525 Transmembrane helix 5 from 96 (out) to 116 (in) : 3379.44 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 16 (in) to 32 (out) : 1228.02 Transmembrane helix 2 from 47 (out) to 62 (in) : 721.31 Transmembrane helix 3 from 66 (in) to 81 (out) : 1548.93 Transmembrane helix 4 from 96 (out) to 116 (in) : 3379.44 Score = 6.810648 Processing 3 helices: Transmembrane helix 1 from 16 (out) to 33 (in) : 1219.73 Transmembrane helix 2 from 51 (in) to 79 (out) : 2605.35 Transmembrane helix 3 from 96 (out) to 116 (in) : 3379.44 Score = 7.152962 Processing 2 helices: Transmembrane helix 1 from 51 (in) to 79 (out) : 2605.35 Transmembrane helix 2 from 96 (out) to 116 (in) : 3379.44 Score = 5.864269 Processing 8 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -3731.09 Transmembrane helix 2 from 26 (in) to 41 (out) : 41.665 Transmembrane helix 3 from 45 (out) to 60 (in) : 459.295 Transmembrane helix 4 from 64 (in) to 79 (out) : 1799.1 Transmembrane helix 5 from 83 (out) to 98 (in) : -241.052 Transmembrane helix 6 from 102 (in) to 117 (out) : 2723.2 Transmembrane helix 7 from 121 (out) to 136 (in) : -849.336 Transmembrane helix 8 from 140 (in) to 155 (out) : -1223.64 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 1220.8 Transmembrane helix 2 from 35 (out) to 50 (in) : -287.804 Transmembrane helix 3 from 54 (in) to 69 (out) : 1602.02 Transmembrane helix 4 from 73 (out) to 88 (in) : 274.275 Transmembrane helix 5 from 95 (in) to 110 (out) : 2806.6 Transmembrane helix 6 from 114 (out) to 129 (in) : -178.035 Transmembrane helix 7 from 133 (in) to 148 (out) : -1033.75 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 1213.2 Transmembrane helix 2 from 35 (in) to 50 (out) : -291.596 Transmembrane helix 3 from 54 (out) to 69 (in) : 1591.38 Transmembrane helix 4 from 73 (in) to 88 (out) : 270.525 Transmembrane helix 5 from 95 (out) to 110 (in) : 2791.7 Transmembrane helix 6 from 114 (in) to 129 (out) : -183.965 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 1220.8 Transmembrane helix 2 from 35 (out) to 50 (in) : -287.804 Transmembrane helix 3 from 54 (in) to 69 (out) : 1602.02 Transmembrane helix 4 from 73 (out) to 88 (in) : 274.275 Transmembrane helix 5 from 96 (in) to 116 (out) : 3409.16 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 16 (out) to 33 (in) : 1219.73 Transmembrane helix 2 from 47 (in) to 62 (out) : 724.29 Transmembrane helix 3 from 66 (out) to 81 (in) : 1564.17 Transmembrane helix 4 from 96 (in) to 116 (out) : 3409.16 Score = 6.985992 Processing 3 helices: Transmembrane helix 1 from 16 (in) to 32 (out) : 1228.02 Transmembrane helix 2 from 51 (out) to 79 (in) : 2614.45 Transmembrane helix 3 from 96 (in) to 116 (out) : 3409.16 Score = 7.304778 Processing 2 helices: Transmembrane helix 1 from 51 (out) to 79 (in) : 2614.45 Transmembrane helix 2 from 96 (in) to 116 (out) : 3409.16 Score = 6.144131 Summary of topology analysis: 1 helix (+) : Score = 3.46351 1 helix (-) : Score = 3.32508 2 helices (+) : Score = 5.86427 2 helices (-) : Score = 6.14413 3 helices (+) : Score = 7.30478 3 helices (-) : Score = 7.15296 4 helices (+) : Score = 6.81065 4 helices (-) : Score = 6.98599 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 15.193 Topology: 16-32,51-79,96-116 Re-entrant helices: Not detected. Helix count: 3 N-terminal: out Score: 7.30478