BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (94 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AAY4 Uncharacterized protein ybjH n=59 Tax=Enterobact...   187   8e-47
UniRef50_C1M9E6 Predicted protein n=2 Tax=Citrobacter RepID=C1M9...   114   8e-25

>UniRef50_P0AAY4 Uncharacterized protein ybjH n=59 Tax=Enterobacteriaceae
          RepID=YBJH_ECOLI
          Length = 94

 Score =  187 bits (475), Expect = 8e-47,   Method: Compositional matrix adjust.
 Identities = 94/94 (100%), Positives = 94/94 (100%)

Query: 1  MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA 60
          MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA
Sbjct: 1  MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA 60

Query: 61 PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH 94
          PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH
Sbjct: 61 PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH 94


>UniRef50_C1M9E6 Predicted protein n=2 Tax=Citrobacter RepID=C1M9E6_9ENTR
          Length = 101

 Score =  114 bits (286), Expect = 8e-25,   Method: Compositional matrix adjust.
 Identities = 75/94 (79%), Positives = 79/94 (84%), Gaps = 2/94 (2%)

Query: 2   IMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVAP 61
           IMK  +L+  LL+GF G   AQSVTVDVPSGYKVVVVP SVSVPQAVSV T PQTVYVAP
Sbjct: 8   IMKKSVLMATLLLGFAGTTFAQSVTVDVPSGYKVVVVPSSVSVPQAVSVTTAPQTVYVAP 67

Query: 62  APAPA--YRPHPYVRHLASVGEGMVIEHQIDDHH 93
           APAPA  YR HPY RH+ASVGEGMVIEHQIDDHH
Sbjct: 68  APAPAPVYRAHPYARHVASVGEGMVIEHQIDDHH 101


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AAY4 Uncharacterized protein ybjH n=59 Tax=Enterobact...   145   3e-34
UniRef50_C1M9E6 Predicted protein n=2 Tax=Citrobacter RepID=C1M9...   115   6e-25

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AAY4 Uncharacterized protein ybjH n=59 Tax=Enterobacteriaceae
          RepID=YBJH_ECOLI
          Length = 94

 Score =  145 bits (367), Expect = 3e-34,   Method: Composition-based stats.
 Identities = 94/94 (100%), Positives = 94/94 (100%)

Query: 1  MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA 60
          MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA
Sbjct: 1  MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA 60

Query: 61 PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH 94
          PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH
Sbjct: 61 PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH 94


>UniRef50_C1M9E6 Predicted protein n=2 Tax=Citrobacter RepID=C1M9E6_9ENTR
          Length = 101

 Score =  115 bits (287), Expect = 6e-25,   Method: Composition-based stats.
 Identities = 74/94 (78%), Positives = 78/94 (82%), Gaps = 2/94 (2%)

Query: 2   IMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYV-- 59
           IMK  +L+  LL+GF G   AQSVTVDVPSGYKVVVVP SVSVPQAVSV T PQTVYV  
Sbjct: 8   IMKKSVLMATLLLGFAGTTFAQSVTVDVPSGYKVVVVPSSVSVPQAVSVTTAPQTVYVAP 67

Query: 60  APAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHH 93
           APAPAP YR HPY RH+ASVGEGMVIEHQIDDHH
Sbjct: 68  APAPAPVYRAHPYARHVASVGEGMVIEHQIDDHH 101


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.313    0.135    0.413 

Lambda     K      H
   0.267   0.0386    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 424,179,762
Number of Sequences: 3077464
Number of extensions: 18740063
Number of successful extensions: 148583
Number of sequences better than 1.0e-01: 13
Number of HSP's better than  0.1 without gapping: 7
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 148506
Number of HSP's gapped (non-prelim): 56
length of query: 94
length of database: 1,040,396,356
effective HSP length: 63
effective length of query: 31
effective length of database: 846,516,124
effective search space: 26241999844
effective search space used: 26241999844
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 87 (38.2 bits)