Processing 1 helix: Transmembrane helix 1 from 150 (in) to 165 (out) : 345.5 Score = 0.345500 Processing 1 helix: Transmembrane helix 1 from 150 (out) to 165 (in) : 345.5 Score = 0.345500 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -84.953 Transmembrane helix 2 from 23 (out) to 38 (in) : -338.7 Transmembrane helix 3 from 42 (in) to 57 (out) : -187.2 Transmembrane helix 4 from 61 (out) to 76 (in) : -43.6 Transmembrane helix 5 from 80 (in) to 95 (out) : -471.05 Transmembrane helix 6 from 99 (out) to 114 (in) : 150.2 Transmembrane helix 7 from 125 (in) to 140 (out) : -412.2 Transmembrane helix 8 from 144 (out) to 159 (in) : 241.6 Transmembrane helix 9 from 163 (in) to 178 (out) : -247.2 Transmembrane helix 10 from 182 (out) to 197 (in) : -311.6 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -80.788 Transmembrane helix 2 from 24 (in) to 39 (out) : -352 Transmembrane helix 3 from 44 (out) to 59 (in) : -149 Transmembrane helix 4 from 63 (in) to 78 (out) : -75.2 Transmembrane helix 5 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 6 from 125 (in) to 140 (out) : -412.2 Transmembrane helix 7 from 144 (out) to 159 (in) : 241.6 Transmembrane helix 8 from 163 (in) to 178 (out) : -247.2 Transmembrane helix 9 from 182 (out) to 197 (in) : -311.6 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -17.944 Transmembrane helix 2 from 26 (out) to 41 (in) : -376.4 Transmembrane helix 3 from 45 (in) to 60 (out) : -132.15 Transmembrane helix 4 from 64 (out) to 79 (in) : -96.85 Transmembrane helix 5 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 6 from 133 (out) to 148 (in) : -178 Transmembrane helix 7 from 152 (in) to 167 (out) : 308.85 Transmembrane helix 8 from 178 (out) to 193 (in) : -288.05 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -67.356 Transmembrane helix 2 from 44 (in) to 59 (out) : -149 Transmembrane helix 3 from 63 (out) to 78 (in) : -75.2 Transmembrane helix 4 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 5 from 133 (out) to 148 (in) : -178 Transmembrane helix 6 from 152 (in) to 167 (out) : 308.85 Transmembrane helix 7 from 178 (out) to 193 (in) : -288.05 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -7.38301 Transmembrane helix 2 from 44 (out) to 59 (in) : -149 Transmembrane helix 3 from 63 (in) to 78 (out) : -75.2 Transmembrane helix 4 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 5 from 133 (in) to 148 (out) : -178 Transmembrane helix 6 from 152 (out) to 167 (in) : 308.85 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -67.356 Transmembrane helix 2 from 44 (in) to 59 (out) : -149 Transmembrane helix 3 from 63 (out) to 78 (in) : -75.2 Transmembrane helix 4 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 5 from 150 (out) to 165 (in) : 345.5 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -7.38301 Transmembrane helix 2 from 58 (out) to 73 (in) : -29.15 Transmembrane helix 3 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 4 from 150 (out) to 165 (in) : 345.5 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 62 (out) to 77 (in) : -57.45 Transmembrane helix 2 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 3 from 150 (out) to 165 (in) : 345.5 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 2 from 150 (out) to 165 (in) : 345.5 Score = 0.594850 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -98.547 Transmembrane helix 2 from 23 (in) to 38 (out) : -338.7 Transmembrane helix 3 from 42 (out) to 57 (in) : -187.2 Transmembrane helix 4 from 61 (in) to 76 (out) : -43.6 Transmembrane helix 5 from 80 (out) to 95 (in) : -471.05 Transmembrane helix 6 from 99 (in) to 114 (out) : 150.2 Transmembrane helix 7 from 125 (out) to 140 (in) : -412.2 Transmembrane helix 8 from 144 (in) to 159 (out) : 241.6 Transmembrane helix 9 from 163 (out) to 178 (in) : -247.2 Transmembrane helix 10 from 182 (in) to 197 (out) : -311.6 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -17.944 Transmembrane helix 2 from 26 (out) to 41 (in) : -376.4 Transmembrane helix 3 from 45 (in) to 60 (out) : -132.15 Transmembrane helix 4 from 64 (out) to 79 (in) : -96.85 Transmembrane helix 5 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 6 from 125 (out) to 140 (in) : -412.2 Transmembrane helix 7 from 144 (in) to 159 (out) : 241.6 Transmembrane helix 8 from 163 (out) to 178 (in) : -247.2 Transmembrane helix 9 from 182 (in) to 197 (out) : -311.6 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -80.788 Transmembrane helix 2 from 24 (in) to 39 (out) : -352 Transmembrane helix 3 from 44 (out) to 59 (in) : -149 Transmembrane helix 4 from 63 (in) to 78 (out) : -75.2 Transmembrane helix 5 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 6 from 133 (in) to 148 (out) : -178 Transmembrane helix 7 from 152 (out) to 167 (in) : 308.85 Transmembrane helix 8 from 178 (in) to 193 (out) : -288.05 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -7.38301 Transmembrane helix 2 from 44 (out) to 59 (in) : -149 Transmembrane helix 3 from 63 (in) to 78 (out) : -75.2 Transmembrane helix 4 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 5 from 133 (in) to 148 (out) : -178 Transmembrane helix 6 from 152 (out) to 167 (in) : 308.85 Transmembrane helix 7 from 178 (in) to 193 (out) : -288.05 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -67.356 Transmembrane helix 2 from 44 (in) to 59 (out) : -149 Transmembrane helix 3 from 63 (out) to 78 (in) : -75.2 Transmembrane helix 4 from 96 (in) to 111 (out) : 249.35 Transmembrane helix 5 from 133 (out) to 148 (in) : -178 Transmembrane helix 6 from 152 (in) to 167 (out) : 308.85 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -7.38301 Transmembrane helix 2 from 44 (out) to 59 (in) : -149 Transmembrane helix 3 from 63 (in) to 78 (out) : -75.2 Transmembrane helix 4 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 5 from 150 (in) to 165 (out) : 345.5 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -67.356 Transmembrane helix 2 from 58 (in) to 73 (out) : -29.15 Transmembrane helix 3 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 4 from 150 (in) to 165 (out) : 345.5 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 58 (in) to 73 (out) : -29.15 Transmembrane helix 2 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 3 from 150 (in) to 165 (out) : 345.5 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 96 (out) to 111 (in) : 249.35 Transmembrane helix 2 from 150 (in) to 165 (out) : 345.5 Score = 0.594850 Summary of topology analysis: 1 helix (+) : Score = 0.3455 1 helix (-) : Score = 0.3455 2 helices (+) : Score = 0.59485 2 helices (-) : Score = 0.59485 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 96-111,150-165 Re-entrant helices: Not detected. Helix count: 2 N-terminal: in Score: 0.59485