BLASTP 2.2.22 [Sep-27-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= batch____ (24 letters) Database: uniref50.fasta 3,077,464 sequences; 1,040,396,356 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value UniRef50_P0AD91 Tryptophanase leader peptide n=33 Tax=Enterobact... 53 4e-06 >UniRef50_P0AD91 Tryptophanase leader peptide n=33 Tax=Enterobacteriaceae RepID=LPTN_ECO57 Length = 24 Score = 52.8 bits (125), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 24/24 (100%), Positives = 24/24 (100%) Query: 1 MNILHICVTSKWFNIDNKIVDHRP 24 MNILHICVTSKWFNIDNKIVDHRP Sbjct: 1 MNILHICVTSKWFNIDNKIVDHRP 24 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: Sequences not found previously or not previously below threshold: UniRef50_P0AD91 Tryptophanase leader peptide n=33 Tax=Enterobact... 43 0.003 CONVERGED! >UniRef50_P0AD91 Tryptophanase leader peptide n=33 Tax=Enterobacteriaceae RepID=LPTN_ECO57 Length = 24 Score = 43.0 bits (100), Expect = 0.003, Method: Composition-based stats. Identities = 24/24 (100%), Positives = 24/24 (100%) Query: 1 MNILHICVTSKWFNIDNKIVDHRP 24 MNILHICVTSKWFNIDNKIVDHRP Sbjct: 1 MNILHICVTSKWFNIDNKIVDHRP 24 Database: uniref50.fasta Posted date: Mar 8, 2010 10:38 AM Number of letters in database: 1,040,396,356 Number of sequences in database: 3,077,464 Lambda K H 0.330 0.142 0.497 Lambda K H 0.267 0.0454 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 108,448,629 Number of Sequences: 3077464 Number of extensions: 1332392 Number of successful extensions: 3517 Number of sequences better than 1.0e-01: 1 Number of HSP's better than 0.1 without gapping: 2 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 3515 Number of HSP's gapped (non-prelim): 2 length of query: 24 length of database: 1,040,396,356 effective HSP length: 0 effective length of query: 24 effective length of database: 1,040,396,356 effective search space: 24969512544 effective search space used: 24969512544 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 87 (38.0 bits)