Processing 1 helix: Transmembrane helix 1 from 19 (in) to 34 (out) : -16.721 Score = -0.014813 Processing 1 helix: Transmembrane helix 1 from 19 (out) to 34 (in) : -25.379 Score = -0.027287 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -218.587 Transmembrane helix 2 from 23 (out) to 38 (in) : -42.802 Transmembrane helix 3 from 42 (in) to 57 (out) : -465.765 Transmembrane helix 4 from 61 (out) to 76 (in) : -448.229 Transmembrane helix 5 from 90 (in) to 105 (out) : -210.552 Transmembrane helix 6 from 109 (out) to 124 (in) : -223.896 Transmembrane helix 7 from 128 (in) to 143 (out) : -365.98 Transmembrane helix 8 from 147 (out) to 162 (in) : -648.258 Transmembrane helix 9 from 166 (in) to 181 (out) : -503.578 Transmembrane helix 10 from 185 (out) to 200 (in) : -558.58 Transmembrane helix 11 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -235.913 Transmembrane helix 2 from 23 (in) to 38 (out) : -26.098 Transmembrane helix 3 from 42 (out) to 57 (in) : -448.135 Transmembrane helix 4 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 5 from 89 (out) to 104 (in) : -234.498 Transmembrane helix 6 from 108 (in) to 123 (out) : -216.138 Transmembrane helix 7 from 127 (out) to 142 (in) : -335.22 Transmembrane helix 8 from 166 (in) to 181 (out) : -503.578 Transmembrane helix 9 from 185 (out) to 200 (in) : -558.58 Transmembrane helix 10 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -218.587 Transmembrane helix 2 from 23 (out) to 38 (in) : -42.802 Transmembrane helix 3 from 42 (in) to 57 (out) : -465.765 Transmembrane helix 4 from 61 (out) to 76 (in) : -448.229 Transmembrane helix 5 from 90 (in) to 105 (out) : -210.552 Transmembrane helix 6 from 109 (out) to 124 (in) : -223.896 Transmembrane helix 7 from 128 (in) to 143 (out) : -365.98 Transmembrane helix 8 from 170 (out) to 185 (in) : -467.753 Transmembrane helix 9 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -235.913 Transmembrane helix 2 from 23 (in) to 38 (out) : -26.098 Transmembrane helix 3 from 42 (out) to 57 (in) : -448.135 Transmembrane helix 4 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 5 from 90 (out) to 105 (in) : -226.148 Transmembrane helix 6 from 109 (in) to 124 (out) : -216.704 Transmembrane helix 7 from 128 (out) to 143 (in) : -343.82 Transmembrane helix 8 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -73.994 Transmembrane helix 2 from 32 (out) to 47 (in) : -148.336 Transmembrane helix 3 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 4 from 90 (out) to 105 (in) : -226.148 Transmembrane helix 5 from 109 (in) to 124 (out) : -216.704 Transmembrane helix 6 from 128 (out) to 143 (in) : -343.82 Transmembrane helix 7 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -135.963 Transmembrane helix 2 from 29 (in) to 44 (out) : -104.054 Transmembrane helix 3 from 90 (out) to 105 (in) : -226.148 Transmembrane helix 4 from 109 (in) to 124 (out) : -216.704 Transmembrane helix 5 from 128 (out) to 143 (in) : -343.82 Transmembrane helix 6 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -73.994 Transmembrane helix 2 from 32 (out) to 47 (in) : -148.336 Transmembrane helix 3 from 96 (in) to 111 (out) : -172.293 Transmembrane helix 4 from 115 (out) to 130 (in) : -200.85 Transmembrane helix 5 from 204 (in) to 219 (out) : -305.837 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -135.963 Transmembrane helix 2 from 29 (in) to 44 (out) : -104.054 Transmembrane helix 3 from 96 (out) to 111 (in) : -185.907 Transmembrane helix 4 from 115 (in) to 130 (out) : -182.25 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -16.721 Transmembrane helix 2 from 94 (out) to 109 (in) : -190.988 Transmembrane helix 3 from 115 (in) to 130 (out) : -182.25 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -25.379 Transmembrane helix 2 from 96 (in) to 111 (out) : -172.293 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -235.913 Transmembrane helix 2 from 23 (in) to 38 (out) : -26.098 Transmembrane helix 3 from 42 (out) to 57 (in) : -448.135 Transmembrane helix 4 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 5 from 87 (out) to 102 (in) : -267.552 Transmembrane helix 6 from 106 (in) to 121 (out) : -225.46 Transmembrane helix 7 from 125 (out) to 140 (in) : -308.336 Transmembrane helix 8 from 144 (in) to 159 (out) : -641.837 Transmembrane helix 9 from 166 (out) to 181 (in) : -530.822 Transmembrane helix 10 from 185 (in) to 200 (out) : -550.92 Transmembrane helix 11 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -218.587 Transmembrane helix 2 from 23 (out) to 38 (in) : -42.802 Transmembrane helix 3 from 42 (in) to 57 (out) : -465.765 Transmembrane helix 4 from 61 (out) to 76 (in) : -448.229 Transmembrane helix 5 from 90 (in) to 105 (out) : -210.552 Transmembrane helix 6 from 109 (out) to 124 (in) : -223.896 Transmembrane helix 7 from 128 (in) to 143 (out) : -365.98 Transmembrane helix 8 from 148 (out) to 163 (in) : -638.992 Transmembrane helix 9 from 170 (in) to 185 (out) : -457.847 Transmembrane helix 10 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -235.913 Transmembrane helix 2 from 23 (in) to 38 (out) : -26.098 Transmembrane helix 3 from 42 (out) to 57 (in) : -448.135 Transmembrane helix 4 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 5 from 89 (out) to 104 (in) : -234.498 Transmembrane helix 6 from 108 (in) to 123 (out) : -216.138 Transmembrane helix 7 from 127 (out) to 142 (in) : -335.22 Transmembrane helix 8 from 170 (in) to 185 (out) : -457.847 Transmembrane helix 9 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -73.994 Transmembrane helix 2 from 32 (out) to 47 (in) : -148.336 Transmembrane helix 3 from 61 (in) to 76 (out) : -444.871 Transmembrane helix 4 from 89 (out) to 104 (in) : -234.498 Transmembrane helix 5 from 108 (in) to 123 (out) : -216.138 Transmembrane helix 6 from 127 (out) to 142 (in) : -335.22 Transmembrane helix 7 from 170 (in) to 185 (out) : -457.847 Transmembrane helix 8 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -135.963 Transmembrane helix 2 from 29 (in) to 44 (out) : -104.054 Transmembrane helix 3 from 89 (out) to 104 (in) : -234.498 Transmembrane helix 4 from 108 (in) to 123 (out) : -216.138 Transmembrane helix 5 from 127 (out) to 142 (in) : -335.22 Transmembrane helix 6 from 170 (in) to 185 (out) : -457.847 Transmembrane helix 7 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -73.994 Transmembrane helix 2 from 32 (out) to 47 (in) : -148.336 Transmembrane helix 3 from 96 (in) to 111 (out) : -172.293 Transmembrane helix 4 from 115 (out) to 130 (in) : -200.85 Transmembrane helix 5 from 170 (in) to 185 (out) : -457.847 Transmembrane helix 6 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -135.963 Transmembrane helix 2 from 29 (in) to 44 (out) : -104.054 Transmembrane helix 3 from 96 (out) to 111 (in) : -185.907 Transmembrane helix 4 from 115 (in) to 130 (out) : -182.25 Transmembrane helix 5 from 204 (out) to 219 (in) : -288.963 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -73.994 Transmembrane helix 2 from 32 (out) to 47 (in) : -148.336 Transmembrane helix 3 from 96 (in) to 111 (out) : -172.293 Transmembrane helix 4 from 115 (out) to 130 (in) : -200.85 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -25.379 Transmembrane helix 2 from 96 (in) to 111 (out) : -172.293 Transmembrane helix 3 from 115 (out) to 130 (in) : -200.85 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -16.721 Transmembrane helix 2 from 94 (out) to 109 (in) : -190.988 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.014813 1 helix (-) : Score = -0.027287 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 19-34 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: -0.014813