Processing 1 helix: Transmembrane helix 1 from 99 (in) to 126 (out) : 4001.45 Score = 4.001451 Processing 1 helix: Transmembrane helix 1 from 100 (out) to 126 (in) : 3959.36 Score = 3.959363 Processing 14 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -68.412 Transmembrane helix 2 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 3 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 4 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 5 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 6 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 7 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 8 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 9 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 10 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 11 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 12 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 13 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 14 from 1 (out) to 0 (in) : 2.071 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -1026.68 Transmembrane helix 2 from 23 (in) to 38 (out) : 1283.33 Transmembrane helix 3 from 42 (out) to 57 (in) : 416.926 Transmembrane helix 4 from 61 (in) to 76 (out) : 1740.41 Transmembrane helix 5 from 80 (out) to 95 (in) : 164.013 Transmembrane helix 6 from 99 (in) to 114 (out) : 2044.93 Transmembrane helix 7 from 118 (out) to 133 (in) : 1310.82 Transmembrane helix 8 from 137 (in) to 152 (out) : -107.865 Transmembrane helix 9 from 156 (out) to 171 (in) : 1853.35 Transmembrane helix 10 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 11 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 12 from 216 (in) to 231 (out) : 927.358 Transmembrane helix 13 from 235 (out) to 250 (in) : 1160.54 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : 1045.06 Transmembrane helix 2 from 41 (out) to 56 (in) : 565.524 Transmembrane helix 3 from 60 (in) to 75 (out) : 1772.68 Transmembrane helix 4 from 79 (out) to 94 (in) : 209.408 Transmembrane helix 5 from 98 (in) to 113 (out) : 1827.03 Transmembrane helix 6 from 117 (out) to 132 (in) : 1569.38 Transmembrane helix 7 from 136 (in) to 151 (out) : -184.284 Transmembrane helix 8 from 155 (out) to 171 (in) : 1953.29 Transmembrane helix 9 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 10 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 11 from 216 (in) to 231 (out) : 927.358 Transmembrane helix 12 from 235 (out) to 250 (in) : 1160.54 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 57 (in) to 73 (out) : 1701.64 Transmembrane helix 3 from 77 (out) to 92 (in) : 304.827 Transmembrane helix 4 from 96 (in) to 111 (out) : 1407.13 Transmembrane helix 5 from 115 (out) to 130 (in) : 2049.14 Transmembrane helix 6 from 135 (in) to 150 (out) : -251.988 Transmembrane helix 7 from 154 (out) to 171 (in) : 2027.78 Transmembrane helix 8 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 9 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 10 from 216 (in) to 231 (out) : 927.358 Transmembrane helix 11 from 235 (out) to 250 (in) : 1160.54 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 57 (out) to 73 (in) : 1714.56 Transmembrane helix 3 from 77 (in) to 92 (out) : 299.573 Transmembrane helix 4 from 96 (out) to 111 (in) : 1396.77 Transmembrane helix 5 from 115 (in) to 130 (out) : 2053.36 Transmembrane helix 6 from 135 (out) to 150 (in) : -245.112 Transmembrane helix 7 from 154 (in) to 172 (out) : 2023.11 Transmembrane helix 8 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 9 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 10 from 220 (out) to 247 (in) : 2637.3 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 57 (in) to 74 (out) : 1768.75 Transmembrane helix 3 from 78 (out) to 93 (in) : 252.25 Transmembrane helix 4 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 5 from 135 (out) to 150 (in) : -245.112 Transmembrane helix 6 from 154 (in) to 172 (out) : 2023.11 Transmembrane helix 7 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 8 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 9 from 220 (out) to 247 (in) : 2637.3 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 57 (out) to 74 (in) : 1780.65 Transmembrane helix 3 from 78 (in) to 93 (out) : 245.45 Transmembrane helix 4 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 5 from 151 (in) to 172 (out) : 2115.9 Transmembrane helix 6 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 7 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 8 from 220 (out) to 247 (in) : 2637.3 Score = 15.738147 Processing 7 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 57 (in) to 74 (out) : 1768.75 Transmembrane helix 3 from 78 (out) to 93 (in) : 252.25 Transmembrane helix 4 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 5 from 151 (out) to 171 (in) : 2129.79 Transmembrane helix 6 from 180 (in) to 210 (out) : 2619.34 Transmembrane helix 7 from 220 (out) to 247 (in) : 2637.3 Score = 15.930053 Processing 6 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 59 (out) to 89 (in) : 2089.41 Transmembrane helix 3 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 4 from 151 (out) to 171 (in) : 2129.79 Transmembrane helix 5 from 180 (in) to 210 (out) : 2619.34 Transmembrane helix 6 from 220 (out) to 247 (in) : 2637.3 Score = 16.117220 Processing 5 helices: Transmembrane helix 1 from 34 (out) to 64 (in) : 2474.64 Transmembrane helix 2 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 3 from 151 (out) to 171 (in) : 2129.79 Transmembrane helix 4 from 180 (in) to 210 (out) : 2619.34 Transmembrane helix 5 from 220 (out) to 247 (in) : 2637.3 Score = 13.914271 Processing 4 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 3 from 180 (in) to 210 (out) : 2619.34 Transmembrane helix 4 from 220 (out) to 247 (in) : 2637.3 Score = 11.773555 Processing 3 helices: Transmembrane helix 1 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 2 from 180 (in) to 210 (out) : 2619.34 Transmembrane helix 3 from 220 (out) to 247 (in) : 2637.3 Score = 9.219447 Processing 2 helices: Transmembrane helix 1 from 99 (in) to 126 (out) : 4001.45 Transmembrane helix 2 from 220 (out) to 247 (in) : 2637.3 Score = 6.654061 Processing 14 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -66.618 Transmembrane helix 2 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 3 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 4 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 5 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 6 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 7 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 8 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 9 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 10 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 11 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 12 from 1 (in) to 0 (out) : -1.897 Transmembrane helix 13 from 1 (out) to 0 (in) : 2.071 Transmembrane helix 14 from 1 (in) to 0 (out) : -1.897 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -1042.62 Transmembrane helix 2 from 23 (out) to 38 (in) : 1278.07 Transmembrane helix 3 from 42 (in) to 57 (out) : 421.274 Transmembrane helix 4 from 61 (out) to 76 (in) : 1745.59 Transmembrane helix 5 from 80 (in) to 95 (out) : 155.187 Transmembrane helix 6 from 99 (out) to 114 (in) : 2029.57 Transmembrane helix 7 from 118 (in) to 133 (out) : 1316.68 Transmembrane helix 8 from 137 (out) to 152 (in) : -100.835 Transmembrane helix 9 from 156 (in) to 172 (out) : 1855.13 Transmembrane helix 10 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 11 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 12 from 216 (out) to 231 (in) : 952.842 Transmembrane helix 13 from 235 (in) to 250 (out) : 1146.76 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -994.197 Transmembrane helix 2 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 3 from 57 (out) to 73 (in) : 1714.56 Transmembrane helix 4 from 77 (in) to 92 (out) : 299.573 Transmembrane helix 5 from 96 (out) to 111 (in) : 1396.77 Transmembrane helix 6 from 115 (in) to 130 (out) : 2053.36 Transmembrane helix 7 from 135 (out) to 150 (in) : -245.112 Transmembrane helix 8 from 154 (in) to 172 (out) : 2023.11 Transmembrane helix 9 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 10 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 11 from 216 (out) to 231 (in) : 952.842 Transmembrane helix 12 from 235 (in) to 250 (out) : 1146.76 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 57 (out) to 73 (in) : 1714.56 Transmembrane helix 3 from 77 (in) to 92 (out) : 299.573 Transmembrane helix 4 from 96 (out) to 111 (in) : 1396.77 Transmembrane helix 5 from 115 (in) to 130 (out) : 2053.36 Transmembrane helix 6 from 135 (out) to 150 (in) : -245.112 Transmembrane helix 7 from 154 (in) to 172 (out) : 2023.11 Transmembrane helix 8 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 9 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 10 from 216 (out) to 231 (in) : 952.842 Transmembrane helix 11 from 235 (in) to 250 (out) : 1146.76 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 57 (in) to 74 (out) : 1768.75 Transmembrane helix 3 from 78 (out) to 93 (in) : 252.25 Transmembrane helix 4 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 5 from 135 (out) to 150 (in) : -245.112 Transmembrane helix 6 from 154 (in) to 172 (out) : 2023.11 Transmembrane helix 7 from 178 (out) to 193 (in) : 1519.51 Transmembrane helix 8 from 197 (in) to 212 (out) : 954.78 Transmembrane helix 9 from 216 (out) to 231 (in) : 952.842 Transmembrane helix 10 from 235 (in) to 250 (out) : 1146.76 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 57 (out) to 74 (in) : 1780.65 Transmembrane helix 3 from 78 (in) to 93 (out) : 245.45 Transmembrane helix 4 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 5 from 133 (in) to 148 (out) : -327.074 Transmembrane helix 6 from 152 (out) to 171 (in) : 2109.83 Transmembrane helix 7 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 8 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 9 from 220 (in) to 247 (out) : 2591.9 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 57 (in) to 74 (out) : 1768.75 Transmembrane helix 3 from 78 (out) to 93 (in) : 252.25 Transmembrane helix 4 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 5 from 151 (out) to 171 (in) : 2129.79 Transmembrane helix 6 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 7 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 8 from 220 (in) to 247 (out) : 2591.9 Score = 15.654748 Processing 7 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 59 (out) to 89 (in) : 2089.41 Transmembrane helix 3 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 4 from 151 (out) to 171 (in) : 2129.79 Transmembrane helix 5 from 178 (in) to 193 (out) : 1528.99 Transmembrane helix 6 from 197 (out) to 212 (in) : 939.02 Transmembrane helix 7 from 220 (in) to 247 (out) : 2591.9 Score = 15.841916 Processing 6 helices: Transmembrane helix 1 from 29 (out) to 46 (in) : 2462.96 Transmembrane helix 2 from 59 (in) to 89 (out) : 2078.59 Transmembrane helix 3 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 4 from 151 (in) to 172 (out) : 2115.9 Transmembrane helix 5 from 180 (out) to 210 (in) : 2582.66 Transmembrane helix 6 from 220 (in) to 247 (out) : 2591.9 Score = 15.656274 Processing 5 helices: Transmembrane helix 1 from 29 (in) to 46 (out) : 2505.24 Transmembrane helix 2 from 59 (out) to 89 (in) : 2089.41 Transmembrane helix 3 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 4 from 180 (out) to 210 (in) : 2582.66 Transmembrane helix 5 from 220 (in) to 247 (out) : 2591.9 Score = 13.812733 Processing 4 helices: Transmembrane helix 1 from 34 (out) to 64 (in) : 2474.64 Transmembrane helix 2 from 100 (in) to 126 (out) : 4001.24 Transmembrane helix 3 from 180 (out) to 210 (in) : 2582.66 Transmembrane helix 4 from 220 (in) to 247 (out) : 2591.9 Score = 11.609783 Processing 3 helices: Transmembrane helix 1 from 99 (in) to 126 (out) : 4001.45 Transmembrane helix 2 from 180 (out) to 210 (in) : 2582.66 Transmembrane helix 3 from 220 (in) to 247 (out) : 2591.9 Score = 9.172566 Processing 2 helices: Transmembrane helix 1 from 100 (out) to 126 (in) : 3959.36 Transmembrane helix 2 from 180 (in) to 210 (out) : 2619.34 Score = 6.575443 Summary of topology analysis: 1 helix (+) : Score = 4.00145 1 helix (-) : Score = 3.95936 2 helices (+) : Score = 6.65406 2 helices (-) : Score = 6.57544 3 helices (+) : Score = 9.17257 3 helices (-) : Score = 9.21945 4 helices (+) : Score = 11.7736 4 helices (-) : Score = 11.6098 5 helices (+) : Score = 13.8127 5 helices (-) : Score = 13.9143 6 helices (+) : Score = 16.1172 6 helices (-) : Score = 15.6563 7 helices (+) : Score = 15.8419 7 helices (-) : Score = 15.9301 8 helices (+) : Score = 15.7381 8 helices (-) : Score = 15.6547 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 29-46,59-89,100-126,151-171,180-210,220-247 Re-entrant helices: Not detected. Helix count: 6 N-terminal: in Score: 16.1172