Processing 1 helix: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Score = 4.198083 Processing 1 helix: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Score = 4.052318 Processing 20 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -5471.73 Transmembrane helix 2 from 26 (out) to 41 (in) : 2069.24 Transmembrane helix 3 from 45 (in) to 60 (out) : -639.824 Transmembrane helix 4 from 64 (out) to 79 (in) : -436.966 Transmembrane helix 5 from 83 (in) to 98 (out) : -889.776 Transmembrane helix 6 from 102 (out) to 117 (in) : -1700.9 Transmembrane helix 7 from 121 (in) to 136 (out) : -1666.98 Transmembrane helix 8 from 140 (out) to 155 (in) : -1566.86 Transmembrane helix 9 from 159 (in) to 174 (out) : -1573.85 Transmembrane helix 10 from 178 (out) to 193 (in) : -1953.36 Transmembrane helix 11 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 12 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 13 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 14 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 15 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 16 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 17 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 18 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 19 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 20 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : 4078.24 Transmembrane helix 2 from 38 (in) to 53 (out) : -948.187 Transmembrane helix 3 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 4 from 76 (in) to 91 (out) : -648.142 Transmembrane helix 5 from 95 (out) to 110 (in) : -1419.09 Transmembrane helix 6 from 115 (in) to 130 (out) : -1779.32 Transmembrane helix 7 from 134 (out) to 149 (in) : -1530.06 Transmembrane helix 8 from 153 (in) to 168 (out) : -1526.3 Transmembrane helix 9 from 172 (out) to 187 (in) : -1891.14 Transmembrane helix 10 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 11 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 12 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 13 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 14 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 15 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 16 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 17 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 18 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 19 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : 4119.96 Transmembrane helix 2 from 38 (out) to 53 (in) : -946.013 Transmembrane helix 3 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 4 from 76 (out) to 91 (in) : -640.758 Transmembrane helix 5 from 95 (in) to 110 (out) : -1414 Transmembrane helix 6 from 125 (out) to 140 (in) : -1560.31 Transmembrane helix 7 from 144 (in) to 159 (out) : -1569.49 Transmembrane helix 8 from 163 (out) to 178 (in) : -1634.29 Transmembrane helix 9 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 10 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 11 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 12 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 13 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 14 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 15 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 16 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 17 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 18 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : 4078.24 Transmembrane helix 2 from 38 (in) to 53 (out) : -948.187 Transmembrane helix 3 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 4 from 76 (in) to 91 (out) : -648.142 Transmembrane helix 5 from 95 (out) to 110 (in) : -1419.09 Transmembrane helix 6 from 133 (in) to 148 (out) : -1534.3 Transmembrane helix 7 from 153 (out) to 168 (in) : -1530.8 Transmembrane helix 8 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 9 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 10 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 11 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 12 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 13 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 14 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 15 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 16 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 17 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : 4119.96 Transmembrane helix 2 from 38 (out) to 53 (in) : -946.013 Transmembrane helix 3 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 4 from 76 (out) to 91 (in) : -640.758 Transmembrane helix 5 from 95 (in) to 110 (out) : -1414 Transmembrane helix 6 from 129 (out) to 144 (in) : -1521.84 Transmembrane helix 7 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 8 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 9 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 10 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 11 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 12 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 13 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 14 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 15 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 16 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : 4078.24 Transmembrane helix 2 from 38 (in) to 53 (out) : -948.187 Transmembrane helix 3 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 4 from 76 (in) to 91 (out) : -648.142 Transmembrane helix 5 from 95 (out) to 110 (in) : -1419.09 Transmembrane helix 6 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 7 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 8 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 9 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 10 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 11 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 12 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 13 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 14 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 15 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 3 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 4 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 5 from 197 (in) to 212 (out) : -933.51 Transmembrane helix 6 from 216 (out) to 231 (in) : -355.613 Transmembrane helix 7 from 235 (in) to 250 (out) : -374.793 Transmembrane helix 8 from 254 (out) to 269 (in) : -492.484 Transmembrane helix 9 from 273 (in) to 288 (out) : -671.718 Transmembrane helix 10 from 292 (out) to 307 (in) : -1188.48 Transmembrane helix 11 from 311 (in) to 326 (out) : -949.142 Transmembrane helix 12 from 330 (out) to 345 (in) : -830.739 Transmembrane helix 13 from 349 (in) to 364 (out) : -1271.69 Transmembrane helix 14 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 45 (in) to 60 (out) : -639.824 Transmembrane helix 3 from 64 (out) to 79 (in) : -436.966 Transmembrane helix 4 from 83 (in) to 98 (out) : -889.776 Transmembrane helix 5 from 204 (out) to 219 (in) : -537.863 Transmembrane helix 6 from 223 (in) to 238 (out) : -328.346 Transmembrane helix 7 from 242 (out) to 257 (in) : -548.62 Transmembrane helix 8 from 261 (in) to 276 (out) : -482.515 Transmembrane helix 9 from 280 (out) to 295 (in) : -831.275 Transmembrane helix 10 from 303 (in) to 318 (out) : -997.576 Transmembrane helix 11 from 322 (out) to 337 (in) : -837.085 Transmembrane helix 12 from 341 (in) to 356 (out) : -1146.34 Transmembrane helix 13 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 3 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 4 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 5 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 6 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 7 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 8 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 9 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 10 from 307 (out) to 322 (in) : -976.635 Transmembrane helix 11 from 326 (in) to 341 (out) : -769.438 Transmembrane helix 12 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 5 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 6 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 7 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 8 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 9 from 307 (out) to 322 (in) : -976.635 Transmembrane helix 10 from 326 (in) to 341 (out) : -769.438 Transmembrane helix 11 from 368 (out) to 383 (in) : -1099.9 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 3 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 4 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 5 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 6 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 7 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 8 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 9 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 10 from 326 (out) to 341 (in) : -773.462 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 5 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 6 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 7 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 8 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 9 from 326 (out) to 341 (in) : -773.462 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 50 (out) to 65 (in) : -468.07 Transmembrane helix 3 from 69 (in) to 84 (out) : -482 Transmembrane helix 4 from 209 (out) to 224 (in) : -400.922 Transmembrane helix 5 from 228 (in) to 243 (out) : -294.79 Transmembrane helix 6 from 247 (out) to 262 (in) : -567.876 Transmembrane helix 7 from 266 (in) to 281 (out) : -532.487 Transmembrane helix 8 from 326 (out) to 341 (in) : -773.462 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 209 (in) to 224 (out) : -397.178 Transmembrane helix 5 from 228 (out) to 243 (in) : -299.91 Transmembrane helix 6 from 247 (in) to 262 (out) : -565.524 Transmembrane helix 7 from 266 (out) to 281 (in) : -526.513 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 50 (out) to 65 (in) : -468.07 Transmembrane helix 3 from 69 (in) to 84 (out) : -482 Transmembrane helix 4 from 210 (out) to 225 (in) : -391.24 Transmembrane helix 5 from 229 (in) to 244 (out) : -299.688 Transmembrane helix 6 from 259 (out) to 274 (in) : -456.339 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 3 from 210 (out) to 225 (in) : -391.24 Transmembrane helix 4 from 229 (in) to 244 (out) : -299.688 Transmembrane helix 5 from 259 (out) to 274 (in) : -456.339 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 3 from 210 (in) to 225 (out) : -389.26 Transmembrane helix 4 from 229 (out) to 244 (in) : -299.612 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 210 (in) to 225 (out) : -389.26 Transmembrane helix 3 from 229 (out) to 244 (in) : -299.612 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 229 (out) to 244 (in) : -299.612 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -5478.47 Transmembrane helix 2 from 26 (in) to 41 (out) : 2074.06 Transmembrane helix 3 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 4 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 5 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 6 from 102 (in) to 117 (out) : -1699 Transmembrane helix 7 from 121 (out) to 136 (in) : -1662.92 Transmembrane helix 8 from 140 (in) to 155 (out) : -1556.94 Transmembrane helix 9 from 159 (out) to 174 (in) : -1580.35 Transmembrane helix 10 from 178 (in) to 193 (out) : -1954.34 Transmembrane helix 11 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 12 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 13 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 14 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 15 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 16 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 17 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 18 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 19 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 20 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : 4119.96 Transmembrane helix 2 from 38 (out) to 53 (in) : -946.013 Transmembrane helix 3 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 4 from 76 (out) to 91 (in) : -640.758 Transmembrane helix 5 from 95 (in) to 110 (out) : -1414 Transmembrane helix 6 from 115 (out) to 130 (in) : -1775.68 Transmembrane helix 7 from 134 (in) to 149 (out) : -1539.54 Transmembrane helix 8 from 153 (out) to 168 (in) : -1530.8 Transmembrane helix 9 from 172 (in) to 187 (out) : -1890.76 Transmembrane helix 10 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 11 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 12 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 13 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 14 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 15 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 16 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 17 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 18 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 19 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : 4078.24 Transmembrane helix 2 from 38 (in) to 53 (out) : -948.187 Transmembrane helix 3 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 4 from 76 (in) to 91 (out) : -648.142 Transmembrane helix 5 from 95 (out) to 110 (in) : -1419.09 Transmembrane helix 6 from 123 (in) to 138 (out) : -1605.32 Transmembrane helix 7 from 142 (out) to 157 (in) : -1579.09 Transmembrane helix 8 from 161 (in) to 176 (out) : -1601.27 Transmembrane helix 9 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 10 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 11 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 12 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 13 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 14 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 15 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 16 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 17 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 18 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : 4119.96 Transmembrane helix 2 from 38 (out) to 53 (in) : -946.013 Transmembrane helix 3 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 4 from 76 (out) to 91 (in) : -640.758 Transmembrane helix 5 from 95 (in) to 110 (out) : -1414 Transmembrane helix 6 from 129 (out) to 144 (in) : -1521.84 Transmembrane helix 7 from 153 (in) to 168 (out) : -1526.3 Transmembrane helix 8 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 9 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 10 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 11 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 12 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 13 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 14 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 15 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 16 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 17 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : 4078.24 Transmembrane helix 2 from 38 (in) to 53 (out) : -948.187 Transmembrane helix 3 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 4 from 76 (in) to 91 (out) : -648.142 Transmembrane helix 5 from 95 (out) to 110 (in) : -1419.09 Transmembrane helix 6 from 153 (in) to 168 (out) : -1526.3 Transmembrane helix 7 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 8 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 9 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 10 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 11 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 12 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 13 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 14 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 15 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 16 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : 4119.96 Transmembrane helix 2 from 38 (out) to 53 (in) : -946.013 Transmembrane helix 3 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 4 from 76 (out) to 91 (in) : -640.758 Transmembrane helix 5 from 95 (in) to 110 (out) : -1414 Transmembrane helix 6 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 7 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 8 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 9 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 10 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 11 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 12 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 13 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 14 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 15 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 45 (in) to 60 (out) : -639.824 Transmembrane helix 3 from 64 (out) to 79 (in) : -436.966 Transmembrane helix 4 from 83 (in) to 98 (out) : -889.776 Transmembrane helix 5 from 197 (out) to 212 (in) : -935.49 Transmembrane helix 6 from 216 (in) to 231 (out) : -360.687 Transmembrane helix 7 from 235 (out) to 250 (in) : -374.607 Transmembrane helix 8 from 254 (in) to 269 (out) : -513.216 Transmembrane helix 9 from 273 (out) to 288 (in) : -674.382 Transmembrane helix 10 from 292 (in) to 307 (out) : -1178.42 Transmembrane helix 11 from 311 (out) to 326 (in) : -949.858 Transmembrane helix 12 from 330 (in) to 345 (out) : -827.061 Transmembrane helix 13 from 349 (out) to 364 (in) : -1279.11 Transmembrane helix 14 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 3 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 4 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 5 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 6 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 7 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 8 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 9 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 10 from 303 (out) to 318 (in) : -994.024 Transmembrane helix 11 from 322 (in) to 337 (out) : -838.615 Transmembrane helix 12 from 341 (out) to 356 (in) : -1143.86 Transmembrane helix 13 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 45 (in) to 60 (out) : -639.824 Transmembrane helix 3 from 64 (out) to 79 (in) : -436.966 Transmembrane helix 4 from 83 (in) to 98 (out) : -889.776 Transmembrane helix 5 from 204 (out) to 219 (in) : -537.863 Transmembrane helix 6 from 223 (in) to 238 (out) : -328.346 Transmembrane helix 7 from 242 (out) to 257 (in) : -548.62 Transmembrane helix 8 from 261 (in) to 276 (out) : -482.515 Transmembrane helix 9 from 280 (out) to 295 (in) : -831.275 Transmembrane helix 10 from 305 (in) to 320 (out) : -976.965 Transmembrane helix 11 from 326 (out) to 341 (in) : -773.462 Transmembrane helix 12 from 368 (in) to 383 (out) : -1100.3 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 45 (out) to 60 (in) : -635.476 Transmembrane helix 3 from 64 (in) to 79 (out) : -427.034 Transmembrane helix 4 from 83 (out) to 98 (in) : -890.124 Transmembrane helix 5 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 6 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 7 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 8 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 9 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 10 from 307 (out) to 322 (in) : -976.635 Transmembrane helix 11 from 326 (in) to 341 (out) : -769.438 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 203 (in) to 218 (out) : -574.146 Transmembrane helix 5 from 222 (out) to 237 (in) : -356.884 Transmembrane helix 6 from 241 (in) to 256 (out) : -521.207 Transmembrane helix 7 from 260 (out) to 275 (in) : -460.584 Transmembrane helix 8 from 279 (in) to 294 (out) : -791.329 Transmembrane helix 9 from 307 (out) to 322 (in) : -976.635 Transmembrane helix 10 from 326 (in) to 341 (out) : -769.438 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 50 (out) to 65 (in) : -468.07 Transmembrane helix 3 from 69 (in) to 84 (out) : -482 Transmembrane helix 4 from 209 (out) to 224 (in) : -400.922 Transmembrane helix 5 from 228 (in) to 243 (out) : -294.79 Transmembrane helix 6 from 247 (out) to 262 (in) : -567.876 Transmembrane helix 7 from 266 (in) to 281 (out) : -532.487 Transmembrane helix 8 from 307 (out) to 322 (in) : -976.635 Transmembrane helix 9 from 326 (in) to 341 (out) : -769.438 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 209 (in) to 224 (out) : -397.178 Transmembrane helix 5 from 228 (out) to 243 (in) : -299.91 Transmembrane helix 6 from 247 (in) to 262 (out) : -565.524 Transmembrane helix 7 from 266 (out) to 281 (in) : -526.513 Transmembrane helix 8 from 326 (in) to 341 (out) : -769.438 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 50 (out) to 65 (in) : -468.07 Transmembrane helix 3 from 69 (in) to 84 (out) : -482 Transmembrane helix 4 from 209 (out) to 224 (in) : -400.922 Transmembrane helix 5 from 228 (in) to 243 (out) : -294.79 Transmembrane helix 6 from 247 (out) to 262 (in) : -567.876 Transmembrane helix 7 from 266 (in) to 281 (out) : -532.487 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 50 (in) to 65 (out) : -473.63 Transmembrane helix 3 from 69 (out) to 84 (in) : -482.2 Transmembrane helix 4 from 212 (in) to 227 (out) : -376.323 Transmembrane helix 5 from 231 (out) to 246 (in) : -312.981 Transmembrane helix 6 from 258 (in) to 273 (out) : -470.596 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 3 from 212 (in) to 227 (out) : -376.323 Transmembrane helix 4 from 231 (out) to 246 (in) : -312.981 Transmembrane helix 5 from 258 (in) to 273 (out) : -470.596 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 57 (in) to 72 (out) : -414.58 Transmembrane helix 3 from 210 (out) to 225 (in) : -391.24 Transmembrane helix 4 from 229 (in) to 244 (out) : -299.688 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 19 (in) to 35 (out) : 4147.28 Transmembrane helix 2 from 57 (out) to 72 (in) : -412.42 Transmembrane helix 3 from 227 (in) to 242 (out) : -292.415 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 19 (out) to 35 (in) : 4103.12 Transmembrane helix 2 from 227 (in) to 242 (out) : -292.415 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 4.19808 1 helix (-) : Score = 4.05232 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 12.478 Topology: 19-35 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 4.19808