BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (89 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P37614 Uncharacterized protein yhhL n=131 Tax=Enterobac...    92   4e-18
UniRef50_Q7MZZ8 Similar to unknown protein YhhL of Escherichia c...    85   5e-16
UniRef50_C2LP10 Putative uncharacterized protein n=1 Tax=Proteus...    83   2e-15
UniRef50_A4TR74 Membrane protein n=27 Tax=Enterobacteriaceae Rep...    82   7e-15
UniRef50_A8FPQ7 Putative uncharacterized protein n=8 Tax=Shewane...    70   2e-11
UniRef50_A1SSK9 Putative uncharacterized protein n=1 Tax=Psychro...    67   2e-10
UniRef50_Q7MQ98 Predicted membrane protein n=59 Tax=Vibrionales ...    65   7e-10
UniRef50_A1SBB6 Putative uncharacterized protein n=10 Tax=Shewan...    51   1e-05
UniRef50_Q1IDY1 Putative uncharacterized protein n=8 Tax=Pseudom...    38   0.074

>UniRef50_P37614 Uncharacterized protein yhhL n=131 Tax=Enterobacteriaceae
          RepID=YHHL_ECOLI
          Length = 89

 Score = 92.3 bits (228), Expect = 4e-18,   Method: Composition-based stats.
 Identities = 89/89 (100%), Positives = 89/89 (100%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP
Sbjct: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89
          QMTTAEKVRIFLFGVFELLAWQKKFKVKK
Sbjct: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89


>UniRef50_Q7MZZ8 Similar to unknown protein YhhL of Escherichia coli n=3
          Tax=Enterobacteriaceae RepID=Q7MZZ8_PHOLL
          Length = 93

 Score = 85.4 bits (210), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 49/89 (55%), Positives = 71/89 (79%), Gaps = 1/89 (1%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          MLI +G+LLM+CVWGFLI NL+HPFP+PL  F+++A+IF V+MH  Q+ LLK++ PKD  
Sbjct: 1  MLIQLGKLLMICVWGFLIFNLIHPFPKPLKYFMDIAMIFMVVMHAFQMLLLKASQPKDQ- 59

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89
          ++++  + +IF FGVFELL+ QKK + +K
Sbjct: 60 KLSSLIQTKIFFFGVFELLSMQKKQRKEK 88


>UniRef50_C2LP10 Putative uncharacterized protein n=1 Tax=Proteus mirabilis ATCC
          29906 RepID=C2LP10_PROMI
          Length = 98

 Score = 83.5 bits (205), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 38/79 (48%), Positives = 58/79 (73%), Gaps = 1/79 (1%)

Query: 10 MLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQMTTAEKVR 69
          M+ VWGFL LNL+ PFP+PL  F+++ALIF V+MHG+Q+A+ K+  P+D  + +   + +
Sbjct: 1  MIGVWGFLCLNLISPFPKPLKYFMDIALIFMVIMHGLQIAMYKAGQPEDK-KPSGLLQFK 59

Query: 70 IFLFGVFELLAWQKKFKVK 88
          IF FG+FELL  QK+ + +
Sbjct: 60 IFFFGIFELLRIQKELRKE 78


>UniRef50_A4TR74 Membrane protein n=27 Tax=Enterobacteriaceae RepID=A4TR74_YERPP
          Length = 90

 Score = 81.5 bits (200), Expect = 7e-15,   Method: Composition-based stats.
 Identities = 54/85 (63%), Positives = 69/85 (81%), Gaps = 1/85 (1%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          M IN+GRLLML VW F++LNL  P+P+PL  F++VA+IF VLMHG+QL LLKST PKD P
Sbjct: 1  MWINLGRLLMLGVWFFILLNLFQPYPKPLRYFIHVAMIFMVLMHGLQLVLLKSTQPKDQP 60

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKF 85
           ++  ++ +IF+FGVFELLAWQKK 
Sbjct: 61 -ISGLQQFKIFVFGVFELLAWQKKQ 84


>UniRef50_A8FPQ7 Putative uncharacterized protein n=8 Tax=Shewanella
          RepID=A8FPQ7_SHESH
          Length = 96

 Score = 70.0 bits (170), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 22/82 (26%), Positives = 38/82 (46%), Gaps = 1/82 (1%)

Query: 3  INIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQM 62
          I  G+ + L  W  +  NL+ PF   + I + + L  T  MH  Q+ +  +   K    +
Sbjct: 5  ITTGKAVTLFAWLLMTYNLIMPFEGNIAIILYILLAITTFMHCFQVVIFHTIF-KSLLSL 63

Query: 63 TTAEKVRIFLFGVFELLAWQKK 84
             +   +F+FG F LL ++ K
Sbjct: 64 KKGDYFSVFIFGAFSLLQYRSK 85


>UniRef50_A1SSK9 Putative uncharacterized protein n=1 Tax=Psychromonas ingrahamii
          37 RepID=A1SSK9_PSYIN
          Length = 88

 Score = 66.5 bits (161), Expect = 2e-10,   Method: Composition-based stats.
 Identities = 30/84 (35%), Positives = 47/84 (55%), Gaps = 1/84 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
           I  G+LLML +WGFLI NL  PFP    +   + L    +MH MQL L+     +   +
Sbjct: 4  FITSGKLLMLAIWGFLIGNLFFPFPGQAALLFYIFLGLISVMHIMQLLLIYGAFAE-KLK 62

Query: 62 MTTAEKVRIFLFGVFELLAWQKKF 85
          +T A+ +++F FGVF++   + + 
Sbjct: 63 LTKADALQVFFFGVFKIWELKGRL 86


>UniRef50_Q7MQ98 Predicted membrane protein n=59 Tax=Vibrionales
          RepID=Q7MQ98_VIBVY
          Length = 88

 Score = 65.0 bits (157), Expect = 7e-10,   Method: Composition-based stats.
 Identities = 26/84 (30%), Positives = 43/84 (51%), Gaps = 1/84 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          L+ + +  +  VW  L+ N+  PFP    I + +   F  +MHG+Q+ +       D   
Sbjct: 4  LLFLAKAAISFVWLILLFNIFSPFPGNAAIVLYIMTAFLFIMHGLQMLIFVGAF-GDKIT 62

Query: 62 MTTAEKVRIFLFGVFELLAWQKKF 85
          MT  EK  I +FG+F LL  ++K+
Sbjct: 63 MTRWEKNSILIFGIFALLDIRRKY 86


>UniRef50_A1SBB6 Putative uncharacterized protein n=10 Tax=Shewanella
          RepID=A1SBB6_SHEAM
          Length = 97

 Score = 51.1 bits (121), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 26/81 (32%), Positives = 41/81 (50%), Gaps = 1/81 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          LI  G+L  L  W  +  NL+ PF   +   + + L  TV+MH  QL +      K  P 
Sbjct: 4  LIMFGKLATLGAWVMMGYNLLTPFDGNIGTLLKILLGVTVVMHSFQLGVFHLLFKKLLPL 63

Query: 62 MTTAEKVRIFLFGVFELLAWQ 82
            + + +++F FGVF LL ++
Sbjct: 64 KAS-DYLQVFAFGVFALLEYR 83


>UniRef50_Q1IDY1 Putative uncharacterized protein n=8 Tax=Pseudomonas
          RepID=Q1IDY1_PSEE4
          Length = 91

 Score = 38.4 bits (88), Expect = 0.074,   Method: Composition-based stats.
 Identities = 20/85 (23%), Positives = 47/85 (55%), Gaps = 2/85 (2%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          ++ +G+ L +  W  +++NL  P P PLN+ +N A IF + +H +++     +L     +
Sbjct: 4  ILGLGKALTVVFWWVVLVNLFMPQPLPLNLLINAAGIFLLTLHVLEILFFNGSLRGRSHR 63

Query: 62 MTTAEKVRIFLFGVFELLAWQKKFK 86
              ++++I L G+F +++  +  +
Sbjct: 64 W--FDRLQILLTGIFHVMSIPRPQE 86


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P37614 Uncharacterized protein yhhL n=131 Tax=Enterobac...    90   2e-17
UniRef50_Q7MZZ8 Similar to unknown protein YhhL of Escherichia c...    83   3e-15
UniRef50_C2LP10 Putative uncharacterized protein n=1 Tax=Proteus...    81   1e-14
UniRef50_A4TR74 Membrane protein n=27 Tax=Enterobacteriaceae Rep...    79   5e-14
UniRef50_A8FPQ7 Putative uncharacterized protein n=8 Tax=Shewane...    70   2e-11
UniRef50_A1SBB6 Putative uncharacterized protein n=10 Tax=Shewan...    66   3e-10
UniRef50_A1SSK9 Putative uncharacterized protein n=1 Tax=Psychro...    64   1e-09
UniRef50_Q7MQ98 Predicted membrane protein n=59 Tax=Vibrionales ...    63   3e-09

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P37614 Uncharacterized protein yhhL n=131 Tax=Enterobacteriaceae
          RepID=YHHL_ECOLI
          Length = 89

 Score = 89.6 bits (221), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 89/89 (100%), Positives = 89/89 (100%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP
Sbjct: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89
          QMTTAEKVRIFLFGVFELLAWQKKFKVKK
Sbjct: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89


>UniRef50_Q7MZZ8 Similar to unknown protein YhhL of Escherichia coli n=3
          Tax=Enterobacteriaceae RepID=Q7MZZ8_PHOLL
          Length = 93

 Score = 83.1 bits (204), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 49/89 (55%), Positives = 71/89 (79%), Gaps = 1/89 (1%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          MLI +G+LLM+CVWGFLI NL+HPFP+PL  F+++A+IF V+MH  Q+ LLK++ PKD  
Sbjct: 1  MLIQLGKLLMICVWGFLIFNLIHPFPKPLKYFMDIAMIFMVVMHAFQMLLLKASQPKDQ- 59

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKFKVKK 89
          ++++  + +IF FGVFELL+ QKK + +K
Sbjct: 60 KLSSLIQTKIFFFGVFELLSMQKKQRKEK 88


>UniRef50_C2LP10 Putative uncharacterized protein n=1 Tax=Proteus mirabilis ATCC
          29906 RepID=C2LP10_PROMI
          Length = 98

 Score = 80.8 bits (198), Expect = 1e-14,   Method: Composition-based stats.
 Identities = 38/79 (48%), Positives = 58/79 (73%), Gaps = 1/79 (1%)

Query: 10 MLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQMTTAEKVR 69
          M+ VWGFL LNL+ PFP+PL  F+++ALIF V+MHG+Q+A+ K+  P+D  + +   + +
Sbjct: 1  MIGVWGFLCLNLISPFPKPLKYFMDIALIFMVIMHGLQIAMYKAGQPEDK-KPSGLLQFK 59

Query: 70 IFLFGVFELLAWQKKFKVK 88
          IF FG+FELL  QK+ + +
Sbjct: 60 IFFFGIFELLRIQKELRKE 78


>UniRef50_A4TR74 Membrane protein n=27 Tax=Enterobacteriaceae RepID=A4TR74_YERPP
          Length = 90

 Score = 78.8 bits (193), Expect = 5e-14,   Method: Composition-based stats.
 Identities = 54/85 (63%), Positives = 69/85 (81%), Gaps = 1/85 (1%)

Query: 1  MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGP 60
          M IN+GRLLML VW F++LNL  P+P+PL  F++VA+IF VLMHG+QL LLKST PKD P
Sbjct: 1  MWINLGRLLMLGVWFFILLNLFQPYPKPLRYFIHVAMIFMVLMHGLQLVLLKSTQPKDQP 60

Query: 61 QMTTAEKVRIFLFGVFELLAWQKKF 85
           ++  ++ +IF+FGVFELLAWQKK 
Sbjct: 61 -ISGLQQFKIFVFGVFELLAWQKKQ 84


>UniRef50_A8FPQ7 Putative uncharacterized protein n=8 Tax=Shewanella
          RepID=A8FPQ7_SHESH
          Length = 96

 Score = 70.0 bits (170), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 22/83 (26%), Positives = 39/83 (46%), Gaps = 1/83 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          +I  G+ + L  W  +  NL+ PF   + I + + L  T  MH  Q+ +  +   K    
Sbjct: 4  VITTGKAVTLFAWLLMTYNLIMPFEGNIAIILYILLAITTFMHCFQVVIFHTIF-KSLLS 62

Query: 62 MTTAEKVRIFLFGVFELLAWQKK 84
          +   +   +F+FG F LL ++ K
Sbjct: 63 LKKGDYFSVFIFGAFSLLQYRSK 85


>UniRef50_A1SBB6 Putative uncharacterized protein n=10 Tax=Shewanella
          RepID=A1SBB6_SHEAM
          Length = 97

 Score = 66.1 bits (160), Expect = 3e-10,   Method: Composition-based stats.
 Identities = 26/81 (32%), Positives = 41/81 (50%), Gaps = 1/81 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          LI  G+L  L  W  +  NL+ PF   +   + + L  TV+MH  QL +      K  P 
Sbjct: 4  LIMFGKLATLGAWVMMGYNLLTPFDGNIGTLLKILLGVTVVMHSFQLGVFHLLFKKLLPL 63

Query: 62 MTTAEKVRIFLFGVFELLAWQ 82
            + + +++F FGVF LL ++
Sbjct: 64 KAS-DYLQVFAFGVFALLEYR 83


>UniRef50_A1SSK9 Putative uncharacterized protein n=1 Tax=Psychromonas ingrahamii
          37 RepID=A1SSK9_PSYIN
          Length = 88

 Score = 63.8 bits (154), Expect = 1e-09,   Method: Composition-based stats.
 Identities = 30/84 (35%), Positives = 47/84 (55%), Gaps = 1/84 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
           I  G+LLML +WGFLI NL  PFP    +   + L    +MH MQL L+     +   +
Sbjct: 4  FITSGKLLMLAIWGFLIGNLFFPFPGQAALLFYIFLGLISVMHIMQLLLIYGAFAE-KLK 62

Query: 62 MTTAEKVRIFLFGVFELLAWQKKF 85
          +T A+ +++F FGVF++   + + 
Sbjct: 63 LTKADALQVFFFGVFKIWELKGRL 86


>UniRef50_Q7MQ98 Predicted membrane protein n=59 Tax=Vibrionales
          RepID=Q7MQ98_VIBVY
          Length = 88

 Score = 63.0 bits (152), Expect = 3e-09,   Method: Composition-based stats.
 Identities = 26/84 (30%), Positives = 43/84 (51%), Gaps = 1/84 (1%)

Query: 2  LINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMHGMQLALLKSTLPKDGPQ 61
          L+ + +  +  VW  L+ N+  PFP    I + +   F  +MHG+Q+ +       D   
Sbjct: 4  LLFLAKAAISFVWLILLFNIFSPFPGNAAIVLYIMTAFLFIMHGLQMLIFVGAF-GDKIT 62

Query: 62 MTTAEKVRIFLFGVFELLAWQKKF 85
          MT  EK  I +FG+F LL  ++K+
Sbjct: 63 MTRWEKNSILIFGIFALLDIRRKY 86


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.328    0.146    0.406 

Lambda     K      H
   0.267   0.0444    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 340,413,906
Number of Sequences: 3077464
Number of extensions: 10145719
Number of successful extensions: 76811
Number of sequences better than 1.0e-01: 19
Number of HSP's better than  0.1 without gapping: 25
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 76760
Number of HSP's gapped (non-prelim): 38
length of query: 89
length of database: 1,040,396,356
effective HSP length: 59
effective length of query: 30
effective length of database: 858,825,980
effective search space: 25764779400
effective search space used: 25764779400
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.2 bits)
S2: 87 (38.0 bits)
