BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (89 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P08339 Putative uncharacterized protein b4223 n=8 Tax=E...   185   5e-46

>UniRef50_P08339 Putative uncharacterized protein b4223 n=8 Tax=Enterobacteriaceae
          RepID=Y4223_ECOLI
          Length = 89

 Score =  185 bits (469), Expect = 5e-46,   Method: Compositional matrix adjust.
 Identities = 89/89 (100%), Positives = 89/89 (100%)

Query: 1  MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ 60
          MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ
Sbjct: 1  MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ 60

Query: 61 YDKLHRVPFRSPELRLDSGPGYTTGSYRY 89
          YDKLHRVPFRSPELRLDSGPGYTTGSYRY
Sbjct: 61 YDKLHRVPFRSPELRLDSGPGYTTGSYRY 89


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P08339 Putative uncharacterized protein b4223 n=8 Tax=E...   158   4e-38

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P08339 Putative uncharacterized protein b4223 n=8 Tax=Enterobacteriaceae
          RepID=Y4223_ECOLI
          Length = 89

 Score =  158 bits (400), Expect = 4e-38,   Method: Composition-based stats.
 Identities = 89/89 (100%), Positives = 89/89 (100%)

Query: 1  MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ 60
          MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ
Sbjct: 1  MHMVTYPCLTSRRFQLALIHRRVVDKRTSMHSRTASESTGARIHRPWCARHQVRPAWRCQ 60

Query: 61 YDKLHRVPFRSPELRLDSGPGYTTGSYRY 89
          YDKLHRVPFRSPELRLDSGPGYTTGSYRY
Sbjct: 61 YDKLHRVPFRSPELRLDSGPGYTTGSYRY 89


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.327    0.135    0.455 

Lambda     K      H
   0.267   0.0415    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 361,669,877
Number of Sequences: 3077464
Number of extensions: 11939337
Number of successful extensions: 26824
Number of sequences better than 1.0e-01: 1
Number of HSP's better than  0.1 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26822
Number of HSP's gapped (non-prelim): 2
length of query: 89
length of database: 1,040,396,356
effective HSP length: 59
effective length of query: 30
effective length of database: 858,825,980
effective search space: 25764779400
effective search space used: 25764779400
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 87 (38.1 bits)