BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (104 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P18032 Protein artA n=10 Tax=Enterobacteriaceae RepID=A...   201   5e-51
UniRef50_B7LIS5 Putative uncharacterized protein artA n=1 Tax=Es...    50   2e-05
UniRef50_Q7WUF1 Putative uncharacterized protein artA n=1 Tax=Es...    50   3e-05

>UniRef50_P18032 Protein artA n=10 Tax=Enterobacteriaceae RepID=ARTA_ECOLI
          Length = 104

 Score =  201 bits (512), Expect = 5e-51,   Method: Compositional matrix adjust.
 Identities = 104/104 (100%), Positives = 104/104 (100%)

Query: 1   MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV 60
           MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV
Sbjct: 1   MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV 60

Query: 61  LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI 104
           LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI
Sbjct: 61  LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI 104


>UniRef50_B7LIS5 Putative uncharacterized protein artA n=1 Tax=Escherichia coli
          ED1a RepID=B7LIS5_ECO81
          Length = 112

 Score = 50.1 bits (118), Expect = 2e-05,   Method: Compositional matrix adjust.
 Identities = 36/86 (41%), Positives = 47/86 (54%), Gaps = 9/86 (10%)

Query: 1  MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAA---- 56
          M+ +  KEKLEIIRNIIRE+LLGNAAI+ L+      + V    ++  IS+L   A    
Sbjct: 1  MKIKQHKEKLEIIRNIIRETLLGNAAIVVLV----SMIIVLCRQEWTFISMLFTGALCLS 56

Query: 57 -GIVVLWLFSIIYIYFCELFRSHWIA 81
             V  W  +II +Y  EL  S  I 
Sbjct: 57 FVFVFFWFIAIIQVYINELTESKRIK 82


>UniRef50_Q7WUF1 Putative uncharacterized protein artA n=1 Tax=Escherichia coli
          RepID=Q7WUF1_ECOLX
          Length = 106

 Score = 49.7 bits (117), Expect = 3e-05,   Method: Compositional matrix adjust.
 Identities = 32/91 (35%), Positives = 57/91 (62%), Gaps = 4/91 (4%)

Query: 1  MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSL--PVNAFPDYLVISLLSIAAGI 58
          M+ +  KEKLEIIR+IIRE+LLGNAA+I L       L  P ++   ++ + ++ ++  +
Sbjct: 1  MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDSM-TFIFMGVVCVSFAV 59

Query: 59 VVLWLFSIIYIYFCELFRSHWIAVW-FIIWS 88
          ++ WL  I+ +Y   L ++ +I ++ F IW+
Sbjct: 60 ILFWLVYIVCLYIGTLVKNIFIRIFAFSIWA 90


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P18032 Protein artA n=10 Tax=Enterobacteriaceae RepID=A...   107   1e-22
UniRef50_Q7WUF1 Putative uncharacterized protein artA n=1 Tax=Es...    73   2e-12
UniRef50_B7LIS5 Putative uncharacterized protein artA n=1 Tax=Es...    68   1e-10

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P18032 Protein artA n=10 Tax=Enterobacteriaceae RepID=ARTA_ECOLI
          Length = 104

 Score =  107 bits (268), Expect = 1e-22,   Method: Composition-based stats.
 Identities = 104/104 (100%), Positives = 104/104 (100%)

Query: 1   MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV 60
           MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV
Sbjct: 1   MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIAAGIVV 60

Query: 61  LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI 104
           LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI
Sbjct: 61  LWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI 104


>UniRef50_Q7WUF1 Putative uncharacterized protein artA n=1 Tax=Escherichia coli
          RepID=Q7WUF1_ECOLX
          Length = 106

 Score = 73.4 bits (179), Expect = 2e-12,   Method: Composition-based stats.
 Identities = 34/100 (34%), Positives = 60/100 (60%), Gaps = 4/100 (4%)

Query: 1  MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSL--PVNAFPDYLVISLLSIAAGI 58
          M+ +  KEKLEIIR+IIRE+LLGNAA+I L       L  P ++   ++ + ++ ++  +
Sbjct: 1  MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDSM-TFIFMGVVCVSFAV 59

Query: 59 VVLWLFSIIYIYFCELFRSHWIAVW-FIIWSSVINLIILY 97
          ++ WL  I+ +Y   L ++ +I ++ F IW+      I+Y
Sbjct: 60 ILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSAEGAIIY 99


>UniRef50_B7LIS5 Putative uncharacterized protein artA n=1 Tax=Escherichia coli
          ED1a RepID=B7LIS5_ECO81
          Length = 112

 Score = 67.6 bits (164), Expect = 1e-10,   Method: Composition-based stats.
 Identities = 36/91 (39%), Positives = 49/91 (53%), Gaps = 9/91 (9%)

Query: 1  MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFPDYLVISLLSIA----- 55
          M+ +  KEKLEIIRNIIRE+LLGNAAI+ L+      + V    ++  IS+L        
Sbjct: 1  MKIKQHKEKLEIIRNIIRETLLGNAAIVVLV----SMIIVLCRQEWTFISMLFTGALCLS 56

Query: 56 AGIVVLWLFSIIYIYFCELFRSHWIAVWFII 86
             V  W  +II +Y  EL  S  I   F++
Sbjct: 57 FVFVFFWFIAIIQVYINELTESKRIKWPFVL 87


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.338    0.151    0.448 

Lambda     K      H
   0.267   0.0457    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 450,308,120
Number of Sequences: 3077464
Number of extensions: 16793207
Number of successful extensions: 139866
Number of sequences better than 1.0e-01: 7
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 139854
Number of HSP's gapped (non-prelim): 10
length of query: 104
length of database: 1,040,396,356
effective HSP length: 72
effective length of query: 32
effective length of database: 818,818,948
effective search space: 26202206336
effective search space used: 26202206336
T: 11
A: 40
X1: 16 ( 7.8 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 87 (38.0 bits)
