Processing 1 helix: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Score = 0.939174 Processing 1 helix: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Score = 0.993626 Processing 16 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -7024.85 Transmembrane helix 2 from 25 (out) to 40 (in) : -84.288 Transmembrane helix 3 from 44 (in) to 59 (out) : -1690.12 Transmembrane helix 4 from 63 (out) to 78 (in) : -1815.83 Transmembrane helix 5 from 82 (in) to 97 (out) : -1551.53 Transmembrane helix 6 from 101 (out) to 116 (in) : -1346.03 Transmembrane helix 7 from 120 (in) to 135 (out) : -1481.95 Transmembrane helix 8 from 139 (out) to 154 (in) : -1153.35 Transmembrane helix 9 from 158 (in) to 173 (out) : 0 Transmembrane helix 10 from 177 (out) to 192 (in) : 0 Transmembrane helix 11 from 196 (in) to 211 (out) : 0 Transmembrane helix 12 from 215 (out) to 230 (in) : 0 Transmembrane helix 13 from 234 (in) to 249 (out) : 0 Transmembrane helix 14 from 253 (out) to 268 (in) : 0 Transmembrane helix 15 from 272 (in) to 287 (out) : 0 Transmembrane helix 16 from 291 (out) to 306 (in) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 58 (out) to 73 (in) : -1745.26 Transmembrane helix 4 from 82 (in) to 97 (out) : -1551.53 Transmembrane helix 5 from 101 (out) to 116 (in) : -1346.03 Transmembrane helix 6 from 120 (in) to 135 (out) : -1481.95 Transmembrane helix 7 from 139 (out) to 154 (in) : -1153.35 Transmembrane helix 8 from 158 (in) to 173 (out) : 0 Transmembrane helix 9 from 177 (out) to 192 (in) : 0 Transmembrane helix 10 from 196 (in) to 211 (out) : 0 Transmembrane helix 11 from 215 (out) to 230 (in) : 0 Transmembrane helix 12 from 234 (in) to 249 (out) : 0 Transmembrane helix 13 from 253 (out) to 268 (in) : 0 Transmembrane helix 14 from 272 (in) to 287 (out) : 0 Transmembrane helix 15 from 291 (out) to 306 (in) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 58 (in) to 73 (out) : -1742.33 Transmembrane helix 4 from 95 (out) to 110 (in) : -1165.87 Transmembrane helix 5 from 120 (in) to 135 (out) : -1481.95 Transmembrane helix 6 from 139 (out) to 154 (in) : -1153.35 Transmembrane helix 7 from 158 (in) to 173 (out) : 0 Transmembrane helix 8 from 177 (out) to 192 (in) : 0 Transmembrane helix 9 from 196 (in) to 211 (out) : 0 Transmembrane helix 10 from 215 (out) to 230 (in) : 0 Transmembrane helix 11 from 234 (in) to 249 (out) : 0 Transmembrane helix 12 from 253 (out) to 268 (in) : 0 Transmembrane helix 13 from 272 (in) to 287 (out) : 0 Transmembrane helix 14 from 291 (out) to 306 (in) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 95 (out) to 110 (in) : -1165.87 Transmembrane helix 4 from 120 (in) to 135 (out) : -1481.95 Transmembrane helix 5 from 139 (out) to 154 (in) : -1153.35 Transmembrane helix 6 from 158 (in) to 173 (out) : 0 Transmembrane helix 7 from 177 (out) to 192 (in) : 0 Transmembrane helix 8 from 196 (in) to 211 (out) : 0 Transmembrane helix 9 from 215 (out) to 230 (in) : 0 Transmembrane helix 10 from 234 (in) to 249 (out) : 0 Transmembrane helix 11 from 253 (out) to 268 (in) : 0 Transmembrane helix 12 from 272 (in) to 287 (out) : 0 Transmembrane helix 13 from 291 (out) to 306 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 95 (in) to 110 (out) : -1182.33 Transmembrane helix 4 from 138 (out) to 153 (in) : -1148.05 Transmembrane helix 5 from 157 (in) to 172 (out) : 0 Transmembrane helix 6 from 176 (out) to 191 (in) : 0 Transmembrane helix 7 from 195 (in) to 210 (out) : 0 Transmembrane helix 8 from 214 (out) to 229 (in) : 0 Transmembrane helix 9 from 233 (in) to 248 (out) : 0 Transmembrane helix 10 from 252 (out) to 267 (in) : 0 Transmembrane helix 11 from 271 (in) to 286 (out) : 0 Transmembrane helix 12 from 290 (out) to 305 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 138 (out) to 153 (in) : -1148.05 Transmembrane helix 4 from 157 (in) to 172 (out) : 0 Transmembrane helix 5 from 176 (out) to 191 (in) : 0 Transmembrane helix 6 from 195 (in) to 210 (out) : 0 Transmembrane helix 7 from 214 (out) to 229 (in) : 0 Transmembrane helix 8 from 233 (in) to 248 (out) : 0 Transmembrane helix 9 from 252 (out) to 267 (in) : 0 Transmembrane helix 10 from 271 (in) to 286 (out) : 0 Transmembrane helix 11 from 290 (out) to 305 (in) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 157 (in) to 172 (out) : 0 Transmembrane helix 4 from 176 (out) to 191 (in) : 0 Transmembrane helix 5 from 195 (in) to 210 (out) : 0 Transmembrane helix 6 from 214 (out) to 229 (in) : 0 Transmembrane helix 7 from 233 (in) to 248 (out) : 0 Transmembrane helix 8 from 252 (out) to 267 (in) : 0 Transmembrane helix 9 from 271 (in) to 286 (out) : 0 Transmembrane helix 10 from 290 (out) to 305 (in) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Transmembrane helix 5 from 214 (out) to 229 (in) : 0 Transmembrane helix 6 from 233 (in) to 248 (out) : 0 Transmembrane helix 7 from 252 (out) to 267 (in) : 0 Transmembrane helix 8 from 271 (in) to 286 (out) : 0 Transmembrane helix 9 from 290 (out) to 305 (in) : 0 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Transmembrane helix 5 from 214 (in) to 229 (out) : 0 Transmembrane helix 6 from 233 (out) to 248 (in) : 0 Transmembrane helix 7 from 252 (in) to 267 (out) : 0 Transmembrane helix 8 from 271 (out) to 286 (in) : 0 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Transmembrane helix 5 from 214 (out) to 229 (in) : 0 Transmembrane helix 6 from 233 (in) to 248 (out) : 0 Transmembrane helix 7 from 252 (out) to 267 (in) : 0 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Transmembrane helix 5 from 214 (in) to 229 (out) : 0 Transmembrane helix 6 from 233 (out) to 248 (in) : 0 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Transmembrane helix 5 from 214 (out) to 229 (in) : 0 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -6993.85 Transmembrane helix 2 from 25 (in) to 40 (out) : -88.112 Transmembrane helix 3 from 44 (out) to 59 (in) : -1698.88 Transmembrane helix 4 from 63 (in) to 78 (out) : -1808.07 Transmembrane helix 5 from 82 (out) to 97 (in) : -1548.07 Transmembrane helix 6 from 101 (in) to 116 (out) : -1357.47 Transmembrane helix 7 from 120 (out) to 135 (in) : -1481.95 Transmembrane helix 8 from 139 (in) to 154 (out) : -1153.35 Transmembrane helix 9 from 158 (out) to 173 (in) : 0 Transmembrane helix 10 from 177 (in) to 192 (out) : 0 Transmembrane helix 11 from 196 (out) to 211 (in) : 0 Transmembrane helix 12 from 215 (in) to 230 (out) : 0 Transmembrane helix 13 from 234 (out) to 249 (in) : 0 Transmembrane helix 14 from 253 (in) to 268 (out) : 0 Transmembrane helix 15 from 272 (out) to 287 (in) : 0 Transmembrane helix 16 from 291 (in) to 306 (out) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 58 (in) to 73 (out) : -1742.33 Transmembrane helix 4 from 82 (out) to 97 (in) : -1548.07 Transmembrane helix 5 from 101 (in) to 116 (out) : -1357.47 Transmembrane helix 6 from 120 (out) to 135 (in) : -1481.95 Transmembrane helix 7 from 139 (in) to 154 (out) : -1153.35 Transmembrane helix 8 from 158 (out) to 173 (in) : 0 Transmembrane helix 9 from 177 (in) to 192 (out) : 0 Transmembrane helix 10 from 196 (out) to 211 (in) : 0 Transmembrane helix 11 from 215 (in) to 230 (out) : 0 Transmembrane helix 12 from 234 (out) to 249 (in) : 0 Transmembrane helix 13 from 253 (in) to 268 (out) : 0 Transmembrane helix 14 from 272 (out) to 287 (in) : 0 Transmembrane helix 15 from 291 (in) to 306 (out) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 82 (out) to 97 (in) : -1548.07 Transmembrane helix 4 from 101 (in) to 116 (out) : -1357.47 Transmembrane helix 5 from 120 (out) to 135 (in) : -1481.95 Transmembrane helix 6 from 139 (in) to 154 (out) : -1153.35 Transmembrane helix 7 from 158 (out) to 173 (in) : 0 Transmembrane helix 8 from 177 (in) to 192 (out) : 0 Transmembrane helix 9 from 196 (out) to 211 (in) : 0 Transmembrane helix 10 from 215 (in) to 230 (out) : 0 Transmembrane helix 11 from 234 (out) to 249 (in) : 0 Transmembrane helix 12 from 253 (in) to 268 (out) : 0 Transmembrane helix 13 from 272 (out) to 287 (in) : 0 Transmembrane helix 14 from 291 (in) to 306 (out) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 95 (in) to 110 (out) : -1182.33 Transmembrane helix 4 from 120 (out) to 135 (in) : -1481.95 Transmembrane helix 5 from 139 (in) to 154 (out) : -1153.35 Transmembrane helix 6 from 158 (out) to 173 (in) : 0 Transmembrane helix 7 from 177 (in) to 192 (out) : 0 Transmembrane helix 8 from 196 (out) to 211 (in) : 0 Transmembrane helix 9 from 215 (in) to 230 (out) : 0 Transmembrane helix 10 from 234 (out) to 249 (in) : 0 Transmembrane helix 11 from 253 (in) to 268 (out) : 0 Transmembrane helix 12 from 272 (out) to 287 (in) : 0 Transmembrane helix 13 from 291 (in) to 306 (out) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 95 (out) to 110 (in) : -1165.87 Transmembrane helix 4 from 138 (in) to 153 (out) : -1148.05 Transmembrane helix 5 from 157 (out) to 172 (in) : 0 Transmembrane helix 6 from 176 (in) to 191 (out) : 0 Transmembrane helix 7 from 195 (out) to 210 (in) : 0 Transmembrane helix 8 from 214 (in) to 229 (out) : 0 Transmembrane helix 9 from 233 (out) to 248 (in) : 0 Transmembrane helix 10 from 252 (in) to 267 (out) : 0 Transmembrane helix 11 from 271 (out) to 286 (in) : 0 Transmembrane helix 12 from 290 (in) to 305 (out) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 34 (out) to 49 (in) : -992.13 Transmembrane helix 3 from 138 (in) to 153 (out) : -1148.05 Transmembrane helix 4 from 157 (out) to 172 (in) : 0 Transmembrane helix 5 from 176 (in) to 191 (out) : 0 Transmembrane helix 6 from 195 (out) to 210 (in) : 0 Transmembrane helix 7 from 214 (in) to 229 (out) : 0 Transmembrane helix 8 from 233 (out) to 248 (in) : 0 Transmembrane helix 9 from 252 (in) to 267 (out) : 0 Transmembrane helix 10 from 271 (out) to 286 (in) : 0 Transmembrane helix 11 from 290 (in) to 305 (out) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 34 (in) to 49 (out) : -995.07 Transmembrane helix 3 from 157 (out) to 172 (in) : 0 Transmembrane helix 4 from 176 (in) to 191 (out) : 0 Transmembrane helix 5 from 195 (out) to 210 (in) : 0 Transmembrane helix 6 from 214 (in) to 229 (out) : 0 Transmembrane helix 7 from 233 (out) to 248 (in) : 0 Transmembrane helix 8 from 252 (in) to 267 (out) : 0 Transmembrane helix 9 from 271 (out) to 286 (in) : 0 Transmembrane helix 10 from 290 (in) to 305 (out) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Transmembrane helix 5 from 214 (in) to 229 (out) : 0 Transmembrane helix 6 from 233 (out) to 248 (in) : 0 Transmembrane helix 7 from 252 (in) to 267 (out) : 0 Transmembrane helix 8 from 271 (out) to 286 (in) : 0 Transmembrane helix 9 from 290 (in) to 305 (out) : 0 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Transmembrane helix 5 from 214 (out) to 229 (in) : 0 Transmembrane helix 6 from 233 (in) to 248 (out) : 0 Transmembrane helix 7 from 252 (out) to 267 (in) : 0 Transmembrane helix 8 from 271 (in) to 286 (out) : 0 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Transmembrane helix 5 from 214 (in) to 229 (out) : 0 Transmembrane helix 6 from 233 (out) to 248 (in) : 0 Transmembrane helix 7 from 252 (in) to 267 (out) : 0 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Transmembrane helix 5 from 214 (out) to 229 (in) : 0 Transmembrane helix 6 from 233 (in) to 248 (out) : 0 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Transmembrane helix 4 from 195 (out) to 210 (in) : 0 Transmembrane helix 5 from 214 (in) to 229 (out) : 0 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Transmembrane helix 3 from 176 (out) to 191 (in) : 0 Transmembrane helix 4 from 195 (in) to 210 (out) : 0 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 962.859 Transmembrane helix 2 from 157 (out) to 172 (in) : 0 Transmembrane helix 3 from 176 (in) to 191 (out) : 0 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 969.941 Transmembrane helix 2 from 157 (in) to 172 (out) : 0 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.939174 1 helix (-) : Score = 0.993626 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: 1-12 Signal score: 24.355 Topology: 15-30 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.993626