Processing 1 helix: Transmembrane helix 1 from 7 (in) to 22 (out) : 231.232 Score = 0.221714 Processing 1 helix: Transmembrane helix 1 from 7 (out) to 22 (in) : 227.468 Score = 0.236986 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 42 (in) to 57 (out) : -1219.29 Transmembrane helix 4 from 68 (out) to 83 (in) : -1033.59 Transmembrane helix 5 from 87 (in) to 102 (out) : -1141.69 Transmembrane helix 6 from 107 (out) to 122 (in) : -880.277 Transmembrane helix 7 from 126 (in) to 141 (out) : -1054 Transmembrane helix 8 from 148 (out) to 163 (in) : -879.765 Transmembrane helix 9 from 167 (in) to 182 (out) : -948.152 Transmembrane helix 10 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 11 from 205 (in) to 220 (out) : -495.71 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 42 (out) to 57 (in) : -1227.31 Transmembrane helix 4 from 74 (in) to 89 (out) : -1014.03 Transmembrane helix 5 from 99 (out) to 114 (in) : -1034.13 Transmembrane helix 6 from 118 (in) to 133 (out) : -734.506 Transmembrane helix 7 from 148 (out) to 163 (in) : -879.765 Transmembrane helix 8 from 167 (in) to 182 (out) : -948.152 Transmembrane helix 9 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 10 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 61 (in) to 70 (in) : 280 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 74 (in) to 89 (out) : -1014.03 Transmembrane helix 4 from 99 (out) to 114 (in) : -1034.13 Transmembrane helix 5 from 118 (in) to 133 (out) : -734.506 Transmembrane helix 6 from 148 (out) to 163 (in) : -879.765 Transmembrane helix 7 from 167 (in) to 182 (out) : -948.152 Transmembrane helix 8 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 9 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 42 (in) to 70 (in) : 291.1 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 99 (out) to 114 (in) : -1034.13 Transmembrane helix 4 from 118 (in) to 133 (out) : -734.506 Transmembrane helix 5 from 148 (out) to 163 (in) : -879.765 Transmembrane helix 6 from 167 (in) to 182 (out) : -948.152 Transmembrane helix 7 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 8 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 61 (out) to 95 (out) : 2030 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 4 from 148 (out) to 163 (in) : -879.765 Transmembrane helix 5 from 167 (in) to 182 (out) : -948.152 Transmembrane helix 6 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 7 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 144 (out) : 630 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 117 (out) to 132 (in) : -726.866 Transmembrane helix 4 from 151 (in) to 166 (out) : -843.582 Transmembrane helix 5 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 6 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 67 (out) to 101 (out) : 2450 Re-entrant helix 2 from 102 (out) to 113 (out) : 840 Re-entrant helix 3 from 136 (in) to 147 (in) : 840 Re-entrant helix 4 from 170 (out) to 182 (out) : 910 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 4 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 5 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 170 (out) : 2450 Re-entrant helix 4 from 171 (out) to 182 (out) : 840 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 227.468 Transmembrane helix 2 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 3 from 186 (out) to 201 (in) : -696.82 Transmembrane helix 4 from 205 (in) to 220 (out) : -495.71 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 170 (out) : 2450 Re-entrant helix 4 from 171 (out) to 182 (out) : 840 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 231.232 Transmembrane helix 2 from 117 (out) to 132 (in) : -726.866 Transmembrane helix 3 from 201 (in) to 216 (out) : -455.582 Re-entrant helix 1 from 67 (out) to 101 (out) : 2450 Re-entrant helix 2 from 102 (out) to 113 (out) : 840 Re-entrant helix 3 from 136 (in) to 170 (in) : 2450 Re-entrant helix 4 from 171 (in) to 197 (in) : 1890 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 227.468 Transmembrane helix 2 from 201 (in) to 216 (out) : -455.582 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 136 (in) : 2450 Re-entrant helix 3 from 137 (in) to 171 (in) : 2450 Re-entrant helix 4 from 172 (in) to 197 (in) : 1820 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 42 (out) to 57 (in) : -1227.31 Transmembrane helix 4 from 61 (in) to 76 (out) : -1258.81 Transmembrane helix 5 from 80 (out) to 95 (in) : -1119.93 Transmembrane helix 6 from 99 (in) to 114 (out) : -1024.17 Transmembrane helix 7 from 118 (out) to 133 (in) : -729.894 Transmembrane helix 8 from 148 (in) to 163 (out) : -880.835 Transmembrane helix 9 from 167 (out) to 182 (in) : -933.148 Transmembrane helix 10 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 11 from 205 (out) to 220 (in) : -486.89 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 42 (in) to 57 (out) : -1219.29 Transmembrane helix 4 from 74 (out) to 89 (in) : -993.469 Transmembrane helix 5 from 99 (in) to 114 (out) : -1024.17 Transmembrane helix 6 from 118 (out) to 133 (in) : -729.894 Transmembrane helix 7 from 148 (in) to 163 (out) : -880.835 Transmembrane helix 8 from 167 (out) to 182 (in) : -933.148 Transmembrane helix 9 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 10 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 61 (out) to 70 (out) : 280 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 74 (out) to 89 (in) : -993.469 Transmembrane helix 4 from 99 (in) to 114 (out) : -1024.17 Transmembrane helix 5 from 118 (out) to 133 (in) : -729.894 Transmembrane helix 6 from 148 (in) to 163 (out) : -880.835 Transmembrane helix 7 from 167 (out) to 182 (in) : -933.148 Transmembrane helix 8 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 9 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 42 (out) to 70 (out) : 291.1 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 100 (in) to 115 (out) : -1024.9 Transmembrane helix 4 from 119 (out) to 134 (in) : -760.137 Transmembrane helix 5 from 148 (in) to 163 (out) : -880.835 Transmembrane helix 6 from 167 (out) to 182 (in) : -933.148 Transmembrane helix 7 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 8 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 62 (in) to 96 (in) : 2100 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 106.598 Transmembrane helix 2 from 23 (in) to 38 (out) : -630.611 Transmembrane helix 3 from 99 (out) to 114 (in) : -1034.13 Transmembrane helix 4 from 118 (in) to 133 (out) : -734.506 Transmembrane helix 5 from 162 (out) to 177 (in) : -841.558 Transmembrane helix 6 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 7 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 61 (out) to 95 (out) : 2030 Re-entrant helix 2 from 137 (out) to 158 (out) : 1540 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 4 from 162 (out) to 177 (in) : -841.558 Transmembrane helix 5 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 6 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 158 (out) : 1610 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 227.468 Transmembrane helix 2 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 3 from 162 (out) to 177 (in) : -841.558 Transmembrane helix 4 from 186 (in) to 201 (out) : -712.68 Transmembrane helix 5 from 205 (out) to 220 (in) : -486.89 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 158 (out) : 1610 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 120.202 Transmembrane helix 2 from 23 (out) to 38 (in) : -626.189 Transmembrane helix 3 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 4 from 202 (out) to 217 (in) : -458.85 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 170 (out) : 2450 Re-entrant helix 4 from 171 (out) to 198 (out) : 1960 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 227.468 Transmembrane helix 2 from 117 (in) to 132 (out) : -733.834 Transmembrane helix 3 from 202 (out) to 217 (in) : -458.85 Re-entrant helix 1 from 67 (in) to 101 (in) : 2450 Re-entrant helix 2 from 102 (in) to 113 (in) : 840 Re-entrant helix 3 from 136 (out) to 170 (out) : 2450 Re-entrant helix 4 from 171 (out) to 198 (out) : 1960 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 231.232 Transmembrane helix 2 from 202 (out) to 217 (in) : -458.85 Re-entrant helix 1 from 67 (out) to 101 (out) : 2450 Re-entrant helix 2 from 102 (out) to 136 (out) : 2450 Re-entrant helix 3 from 137 (out) to 171 (out) : 2450 Re-entrant helix 4 from 172 (out) to 198 (out) : 1890 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.221714 1 helix (-) : Score = 0.236986 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 7-22 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.236986