BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (83 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P07010 Uncharacterized protein rem n=50 Tax=Bacteria Re...   168   4e-41
UniRef50_B5MUF2 Putative uncharacterized protein n=1 Tax=Salmone...    44   0.002
UniRef50_C2DWS1 Putative uncharacterized protein n=2 Tax=Escheri...    42   0.007

>UniRef50_P07010 Uncharacterized protein rem n=50 Tax=Bacteria RepID=REM_ECOLI
          Length = 83

 Score =  168 bits (426), Expect = 4e-41,   Method: Compositional matrix adjust.
 Identities = 83/83 (100%), Positives = 83/83 (100%)

Query: 1  MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE 60
          MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE
Sbjct: 1  MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE 60

Query: 61 FVTSLQALKEIGIKAGFSAFSGE 83
          FVTSLQALKEIGIKAGFSAFSGE
Sbjct: 61 FVTSLQALKEIGIKAGFSAFSGE 83


>UniRef50_B5MUF2 Putative uncharacterized protein n=1 Tax=Salmonella enterica
          subsp. enterica serovar Saintpaul str. SARA29
          RepID=B5MUF2_SALET
          Length = 64

 Score = 43.5 bits (101), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 21/45 (46%), Positives = 25/45 (55%)

Query: 34 CLRKNGAALINFVDARVTDGFILREGEFVTSLQALKEIGIKAGFS 78
           L   G  LI  ++     GF+LRE EFVTS Q L+E G  AGF 
Sbjct: 11 LLTHTGPVLIYLINGEAQRGFLLRENEFVTSWQELQEAGKLAGFP 55


>UniRef50_C2DWS1 Putative uncharacterized protein n=2 Tax=Escherichia
          RepID=C2DWS1_ECOLX
          Length = 73

 Score = 41.6 bits (96), Expect = 0.007,   Method: Compositional matrix adjust.
 Identities = 19/26 (73%), Positives = 22/26 (84%)

Query: 1  MMNIEELRKIFCEDGLYAVCVENGNL 26
          +M IEELR+IF EDGLYAV VENG +
Sbjct: 16 LMKIEELREIFSEDGLYAVRVENGGI 41


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P07010 Uncharacterized protein rem n=50 Tax=Bacteria Re...   165   4e-40

Sequences not found previously or not previously below threshold:

UniRef50_C2DWS1 Putative uncharacterized protein n=2 Tax=Escheri...    42   0.005
UniRef50_B5MUF2 Putative uncharacterized protein n=1 Tax=Salmone...    42   0.006


CONVERGED!
>UniRef50_P07010 Uncharacterized protein rem n=50 Tax=Bacteria RepID=REM_ECOLI
          Length = 83

 Score =  165 bits (418), Expect = 4e-40,   Method: Composition-based stats.
 Identities = 83/83 (100%), Positives = 83/83 (100%)

Query: 1  MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE 60
          MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE
Sbjct: 1  MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALINFVDARVTDGFILREGE 60

Query: 61 FVTSLQALKEIGIKAGFSAFSGE 83
          FVTSLQALKEIGIKAGFSAFSGE
Sbjct: 61 FVTSLQALKEIGIKAGFSAFSGE 83


>UniRef50_C2DWS1 Putative uncharacterized protein n=2 Tax=Escherichia
          RepID=C2DWS1_ECOLX
          Length = 73

 Score = 42.3 bits (98), Expect = 0.005,   Method: Composition-based stats.
 Identities = 19/26 (73%), Positives = 22/26 (84%)

Query: 1  MMNIEELRKIFCEDGLYAVCVENGNL 26
          +M IEELR+IF EDGLYAV VENG +
Sbjct: 16 LMKIEELREIFSEDGLYAVRVENGGI 41


>UniRef50_B5MUF2 Putative uncharacterized protein n=1 Tax=Salmonella enterica
          subsp. enterica serovar Saintpaul str. SARA29
          RepID=B5MUF2_SALET
          Length = 64

 Score = 41.9 bits (97), Expect = 0.006,   Method: Composition-based stats.
 Identities = 21/43 (48%), Positives = 25/43 (58%)

Query: 35 LRKNGAALINFVDARVTDGFILREGEFVTSLQALKEIGIKAGF 77
          L   G  LI  ++     GF+LRE EFVTS Q L+E G  AGF
Sbjct: 12 LTHTGPVLIYLINGEAQRGFLLRENEFVTSWQELQEAGKLAGF 54


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.326    0.142    0.412 

Lambda     K      H
   0.267   0.0463    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 371,280,639
Number of Sequences: 3077464
Number of extensions: 14448588
Number of successful extensions: 33430
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 33424
Number of HSP's gapped (non-prelim): 6
length of query: 83
length of database: 1,040,396,356
effective HSP length: 53
effective length of query: 30
effective length of database: 877,290,764
effective search space: 26318722920
effective search space used: 26318722920
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 87 (37.9 bits)