BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (73 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriacea...   142   4e-33
UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae...    99   4e-20
UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriac...    82   4e-15
UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32...    57   1e-07
UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TI...    47   2e-04
UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nason...    45   6e-04
UniRef50_UPI0001910EB9 hypothetical protein SentesTy_16341 n=1 T...    44   0.002

>UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriaceae
          RepID=PSPD_ECO57
          Length = 73

 Score =  142 bits (357), Expect = 4e-33,   Method: Compositional matrix adjust.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60
          MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL
Sbjct: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60

Query: 61 SRAANKLAQRYKR 73
          SRAANKLAQRYKR
Sbjct: 61 SRAANKLAQRYKR 73


>UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae
          RepID=C9Y589_CROTZ
          Length = 80

 Score = 99.0 bits (245), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 47/70 (67%), Positives = 57/70 (81%)

Query: 2  NTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLS 61
           TRWQ+  ++ KPG K+AGKL L+TALRYGPAG AGWA+KSVARRPLK+ LA ALEPLL 
Sbjct: 4  TTRWQRVSRRAKPGLKIAGKLALMTALRYGPAGFAGWAVKSVARRPLKIALAFALEPLLK 63

Query: 62 RAANKLAQRY 71
          R A +++ RY
Sbjct: 64 RLAKRVSARY 73


>UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriaceae
          RepID=C8Q9S2_9ENTR
          Length = 82

 Score = 82.4 bits (202), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 37/70 (52%), Positives = 53/70 (75%)

Query: 4  RWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRA 63
          R+    ++  P  K AGK ++++A+ Y P G+AGWA+KSVARRPL++LLAVALEPLLS+ 
Sbjct: 5  RFSTLRRRAAPALKSAGKFIIISAVTYAPGGIAGWAVKSVARRPLRLLLAVALEPLLSKV 64

Query: 64 ANKLAQRYKR 73
           N+L+ R+ R
Sbjct: 65 FNRLSARFIR 74


>UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32_SERP5
          Length = 87

 Score = 57.4 bits (137), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 23/51 (45%), Positives = 37/51 (72%)

Query: 21 KLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQRY 71
          K++++  L YGPAG AGW +++V R+P++ +LA+ LEPL  +  NK+  RY
Sbjct: 28 KVIIIALLNYGPAGAAGWLLRTVGRKPIRFVLALVLEPLFRKGLNKVFGRY 78


>UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TIT8_YERPP
          Length = 80

 Score = 47.0 bits (110), Expect = 2e-04,   Method: Compositional matrix adjust.
 Identities = 23/53 (43%), Positives = 35/53 (66%)

Query: 21 KLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQRYKR 73
          K++   AL YGPAG A   +KSV+ +PL+ LL + LEP+L RA   +A ++ +
Sbjct: 20 KIMFTAALMYGPAGAASVIMKSVSYKPLRWLLLIILEPMLKRAMAAVASQFAK 72


>UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nasoniae
          RepID=D2TZ89_9ENTR
          Length = 76

 Score = 45.4 bits (106), Expect = 6e-04,   Method: Compositional matrix adjust.
 Identities = 21/62 (33%), Positives = 37/62 (59%)

Query: 8  AGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKL 67
           GQK K   K   ++ ++ A+ Y PAG+ G  +K ++R+PL+  + +A++PLL     KL
Sbjct: 3  CGQKFKLLSKKMARIAIIMAIHYAPAGIIGLMLKYISRKPLRWFMLIAMQPLLRLGIAKL 62

Query: 68 AQ 69
           +
Sbjct: 63 VR 64


>UniRef50_UPI0001910EB9 hypothetical protein SentesTy_16341 n=1 Tax=Salmonella enterica
          subsp. enterica serovar Typhi str. J185
          RepID=UPI0001910EB9
          Length = 50

 Score = 43.5 bits (101), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 24/36 (66%), Positives = 26/36 (72%), Gaps = 2/36 (5%)

Query: 12 VKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRP 47
          +KPGFK+AGKLVLLTALRYGP    G   K   RRP
Sbjct: 1  MKPGFKIAGKLVLLTALRYGPCRRGGMGGK--IRRP 34


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriacea...    84   1e-15
UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriac...    76   3e-13
UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae...    75   9e-13
UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32...    66   3e-10
UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nason...    65   7e-10
UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TI...    61   1e-08

Sequences not found previously or not previously below threshold:

UniRef50_B4EWK3 Phage shock protein D n=3 Tax=Proteus RepID=B4EW...    44   0.001
UniRef50_UPI0001910EB9 hypothetical protein SentesTy_16341 n=1 T...    40   0.030

>UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriaceae
          RepID=PSPD_ECO57
          Length = 73

 Score = 83.9 bits (206), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60
          MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL
Sbjct: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60

Query: 61 SRAANKLAQRYKR 73
          SRAANKLAQRYKR
Sbjct: 61 SRAANKLAQRYKR 73


>UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriaceae
          RepID=C8Q9S2_9ENTR
          Length = 82

 Score = 76.2 bits (186), Expect = 3e-13,   Method: Composition-based stats.
 Identities = 37/70 (52%), Positives = 53/70 (75%)

Query: 4  RWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRA 63
          R+    ++  P  K AGK ++++A+ Y P G+AGWA+KSVARRPL++LLAVALEPLLS+ 
Sbjct: 5  RFSTLRRRAAPALKSAGKFIIISAVTYAPGGIAGWAVKSVARRPLRLLLAVALEPLLSKV 64

Query: 64 ANKLAQRYKR 73
           N+L+ R+ R
Sbjct: 65 FNRLSARFIR 74


>UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae
          RepID=C9Y589_CROTZ
          Length = 80

 Score = 74.7 bits (182), Expect = 9e-13,   Method: Composition-based stats.
 Identities = 47/71 (66%), Positives = 57/71 (80%)

Query: 2  NTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLS 61
           TRWQ+  ++ KPG K+AGKL L+TALRYGPAG AGWA+KSVARRPLK+ LA ALEPLL 
Sbjct: 4  TTRWQRVSRRAKPGLKIAGKLALMTALRYGPAGFAGWAVKSVARRPLKIALAFALEPLLK 63

Query: 62 RAANKLAQRYK 72
          R A +++ RY 
Sbjct: 64 RLAKRVSARYI 74


>UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32_SERP5
          Length = 87

 Score = 66.2 bits (160), Expect = 3e-10,   Method: Composition-based stats.
 Identities = 25/63 (39%), Positives = 39/63 (61%)

Query: 10 QKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQ 69
          Q      K   K++++  L YGPAG AGW +++V R+P++ +LA+ LEPL  +  NK+  
Sbjct: 17 QGAGAMLKTLSKVIIIALLNYGPAGAAGWLLRTVGRKPIRFVLALVLEPLFRKGLNKVFG 76

Query: 70 RYK 72
          RY 
Sbjct: 77 RYA 79


>UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nasoniae
          RepID=D2TZ89_9ENTR
          Length = 76

 Score = 65.1 bits (157), Expect = 7e-10,   Method: Composition-based stats.
 Identities = 21/62 (33%), Positives = 37/62 (59%)

Query: 8  AGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKL 67
           GQK K   K   ++ ++ A+ Y PAG+ G  +K ++R+PL+  + +A++PLL     KL
Sbjct: 3  CGQKFKLLSKKMARIAIIMAIHYAPAGIIGLMLKYISRKPLRWFMLIAMQPLLRLGIAKL 62

Query: 68 AQ 69
           +
Sbjct: 63 VR 64


>UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TIT8_YERPP
          Length = 80

 Score = 61.2 bits (147), Expect = 1e-08,   Method: Composition-based stats.
 Identities = 23/63 (36%), Positives = 36/63 (57%)

Query: 11 KVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQR 70
          +         K++   AL YGPAG A   +KSV+ +PL+ LL + LEP+L RA   +A +
Sbjct: 10 RAGVILTKLIKIMFTAALMYGPAGAASVIMKSVSYKPLRWLLLIILEPMLKRAMAAVASQ 69

Query: 71 YKR 73
          + +
Sbjct: 70 FAK 72


>UniRef50_B4EWK3 Phage shock protein D n=3 Tax=Proteus RepID=B4EWK3_PROMH
          Length = 85

 Score = 44.3 bits (103), Expect = 0.001,   Method: Composition-based stats.
 Identities = 15/53 (28%), Positives = 28/53 (52%)

Query: 17 KLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQ 69
          K      L     +GPA + G  +K V+ +PL+ L+ +  EP++    +KL++
Sbjct: 21 KKGVTFALTLLTLFGPAAITGGILKRVSYKPLRWLVLLIAEPMIKMGLDKLSR 73


>UniRef50_UPI0001910EB9 hypothetical protein SentesTy_16341 n=1 Tax=Salmonella enterica
          subsp. enterica serovar Typhi str. J185
          RepID=UPI0001910EB9
          Length = 50

 Score = 39.7 bits (91), Expect = 0.030,   Method: Composition-based stats.
 Identities = 24/36 (66%), Positives = 26/36 (72%), Gaps = 2/36 (5%)

Query: 12 VKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRP 47
          +KPGFK+AGKLVLLTALRYGP    G   K   RRP
Sbjct: 1  MKPGFKIAGKLVLLTALRYGPCRRGGMGGK--IRRP 34


Searching..................................................done


Results from round 3


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriacea...    79   5e-14
UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriac...    74   1e-12
UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32...    72   6e-12
UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae...    72   7e-12
UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TI...    67   1e-10
UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nason...    64   1e-09
UniRef50_B4EWK3 Phage shock protein D n=3 Tax=Proteus RepID=B4EW...    61   1e-08

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AFV9 Phage shock protein D n=94 Tax=Enterobacteriaceae
          RepID=PSPD_ECO57
          Length = 73

 Score = 78.5 bits (192), Expect = 5e-14,   Method: Composition-based stats.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60
          MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL
Sbjct: 1  MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLL 60

Query: 61 SRAANKLAQRYKR 73
          SRAANKLAQRYKR
Sbjct: 61 SRAANKLAQRYKR 73


>UniRef50_C8Q9S2 Phage shock protein PspD n=4 Tax=Enterobacteriaceae
          RepID=C8Q9S2_9ENTR
          Length = 82

 Score = 73.9 bits (180), Expect = 1e-12,   Method: Composition-based stats.
 Identities = 37/70 (52%), Positives = 53/70 (75%)

Query: 4  RWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRA 63
          R+    ++  P  K AGK ++++A+ Y P G+AGWA+KSVARRPL++LLAVALEPLLS+ 
Sbjct: 5  RFSTLRRRAAPALKSAGKFIIISAVTYAPGGIAGWAVKSVARRPLRLLLAVALEPLLSKV 64

Query: 64 ANKLAQRYKR 73
           N+L+ R+ R
Sbjct: 65 FNRLSARFIR 74


>UniRef50_A8GF32 Shock protein PspD n=2 Tax=Serratia RepID=A8GF32_SERP5
          Length = 87

 Score = 72.0 bits (175), Expect = 6e-12,   Method: Composition-based stats.
 Identities = 25/63 (39%), Positives = 39/63 (61%)

Query: 10 QKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQ 69
          Q      K   K++++  L YGPAG AGW +++V R+P++ +LA+ LEPL  +  NK+  
Sbjct: 17 QGAGAMLKTLSKVIIIALLNYGPAGAAGWLLRTVGRKPIRFVLALVLEPLFRKGLNKVFG 76

Query: 70 RYK 72
          RY 
Sbjct: 77 RYA 79


>UniRef50_C9Y589 Phage shock protein D n=8 Tax=Enterobacteriaceae
          RepID=C9Y589_CROTZ
          Length = 80

 Score = 71.6 bits (174), Expect = 7e-12,   Method: Composition-based stats.
 Identities = 47/71 (66%), Positives = 57/71 (80%)

Query: 2  NTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLS 61
           TRWQ+  ++ KPG K+AGKL L+TALRYGPAG AGWA+KSVARRPLK+ LA ALEPLL 
Sbjct: 4  TTRWQRVSRRAKPGLKIAGKLALMTALRYGPAGFAGWAVKSVARRPLKIALAFALEPLLK 63

Query: 62 RAANKLAQRYK 72
          R A +++ RY 
Sbjct: 64 RLAKRVSARYI 74


>UniRef50_A4TIT8 Phage shock protein n=28 Tax=Yersinia RepID=A4TIT8_YERPP
          Length = 80

 Score = 67.4 bits (163), Expect = 1e-10,   Method: Composition-based stats.
 Identities = 23/63 (36%), Positives = 36/63 (57%)

Query: 11 KVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQR 70
          +         K++   AL YGPAG A   +KSV+ +PL+ LL + LEP+L RA   +A +
Sbjct: 10 RAGVILTKLIKIMFTAALMYGPAGAASVIMKSVSYKPLRWLLLIILEPMLKRAMAAVASQ 69

Query: 71 YKR 73
          + +
Sbjct: 70 FAK 72


>UniRef50_D2TZ89 Phage shock protein D n=1 Tax=Arsenophonus nasoniae
          RepID=D2TZ89_9ENTR
          Length = 76

 Score = 64.3 bits (155), Expect = 1e-09,   Method: Composition-based stats.
 Identities = 21/62 (33%), Positives = 37/62 (59%)

Query: 8  AGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKL 67
           GQK K   K   ++ ++ A+ Y PAG+ G  +K ++R+PL+  + +A++PLL     KL
Sbjct: 3  CGQKFKLLSKKMARIAIIMAIHYAPAGIIGLMLKYISRKPLRWFMLIAMQPLLRLGIAKL 62

Query: 68 AQ 69
           +
Sbjct: 63 VR 64


>UniRef50_B4EWK3 Phage shock protein D n=3 Tax=Proteus RepID=B4EWK3_PROMH
          Length = 85

 Score = 60.8 bits (146), Expect = 1e-08,   Method: Composition-based stats.
 Identities = 15/53 (28%), Positives = 28/53 (52%)

Query: 17 KLAGKLVLLTALRYGPAGVAGWAIKSVARRPLKMLLAVALEPLLSRAANKLAQ 69
          K      L     +GPA + G  +K V+ +PL+ L+ +  EP++    +KL++
Sbjct: 21 KKGVTFALTLLTLFGPAAITGGILKRVSYKPLRWLVLLIAEPMIKMGLDKLSR 73


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.320    0.136    0.365 

Lambda     K      H
   0.267   0.0420    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 367,685,031
Number of Sequences: 3077464
Number of extensions: 10191906
Number of successful extensions: 32551
Number of sequences better than 1.0e-01: 9
Number of HSP's better than  0.1 without gapping: 25
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 32524
Number of HSP's gapped (non-prelim): 27
length of query: 73
length of database: 1,040,396,356
effective HSP length: 44
effective length of query: 29
effective length of database: 904,987,940
effective search space: 26244650260
effective search space used: 26244650260
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 87 (38.1 bits)