BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (73 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q9S4X1 Uncharacterized protein yubE n=76 Tax=root RepID...   149   4e-35
UniRef50_A6TIQ4 Putative uncharacterized protein n=2 Tax=Klebsie...    55   9e-07

>UniRef50_Q9S4X1 Uncharacterized protein yubE n=76 Tax=root RepID=YUBE_ECOLI
          Length = 73

 Score =  149 bits (375), Expect = 4e-35,   Method: Compositional matrix adjust.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60
          MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA
Sbjct: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60

Query: 61 YTEILELDACFKD 73
          YTEILELDACFKD
Sbjct: 61 YTEILELDACFKD 73


>UniRef50_A6TIQ4 Putative uncharacterized protein n=2 Tax=Klebsiella pneumoniae
          RepID=A6TIQ4_KLEP7
          Length = 73

 Score = 54.7 bits (130), Expect = 9e-07,   Method: Compositional matrix adjust.
 Identities = 32/73 (43%), Positives = 42/73 (57%)

Query: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60
          MNY GH+ LR D+A +AN +CDL+  L  +E    +  D L ERLA  +L R + L  EA
Sbjct: 1  MNYDGHEALRRDMAGLANNLCDLKTTLKVLEDTYHYRYDGLAERLAGISLRRLSVLMDEA 60

Query: 61 YTEILELDACFKD 73
          +   L LD  F D
Sbjct: 61 FNIALMLDESFLD 73


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q9S4X1 Uncharacterized protein yubE n=76 Tax=root RepID...    94   1e-18
UniRef50_A6TIQ4 Putative uncharacterized protein n=2 Tax=Klebsie...    93   2e-18

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q9S4X1 Uncharacterized protein yubE n=76 Tax=root RepID=YUBE_ECOLI
          Length = 73

 Score = 94.3 bits (233), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 73/73 (100%), Positives = 73/73 (100%)

Query: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60
          MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA
Sbjct: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60

Query: 61 YTEILELDACFKD 73
          YTEILELDACFKD
Sbjct: 61 YTEILELDACFKD 73


>UniRef50_A6TIQ4 Putative uncharacterized protein n=2 Tax=Klebsiella pneumoniae
          RepID=A6TIQ4_KLEP7
          Length = 73

 Score = 93.5 bits (231), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 32/73 (43%), Positives = 42/73 (57%)

Query: 1  MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVERLARQTLYRANRLFMEA 60
          MNY GH+ LR D+A +AN +CDL+  L  +E    +  D L ERLA  +L R + L  EA
Sbjct: 1  MNYDGHEALRRDMAGLANNLCDLKTTLKVLEDTYHYRYDGLAERLAGISLRRLSVLMDEA 60

Query: 61 YTEILELDACFKD 73
          +   L LD  F D
Sbjct: 61 FNIALMLDESFLD 73


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.332    0.144    0.391 

Lambda     K      H
   0.267   0.0423    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 260,369,920
Number of Sequences: 3077464
Number of extensions: 7929132
Number of successful extensions: 29948
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 29943
Number of HSP's gapped (non-prelim): 5
length of query: 73
length of database: 1,040,396,356
effective HSP length: 44
effective length of query: 29
effective length of database: 904,987,940
effective search space: 26244650260
effective search space used: 26244650260
T: 11
A: 40
X1: 16 ( 7.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 87 (38.1 bits)