BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (114 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AD55 Uncharacterized protein ygaC n=132 Tax=Enterobac...   236   1e-61
UniRef50_A8ANM7 Putative uncharacterized protein n=11 Tax=Entero...   222   3e-57

>UniRef50_P0AD55 Uncharacterized protein ygaC n=132 Tax=Enterobacteriaceae
           RepID=YGAC_ECO57
          Length = 114

 Score =  236 bits (603), Expect = 1e-61,   Method: Compositional matrix adjust.
 Identities = 114/114 (100%), Positives = 114/114 (100%)

Query: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60
           MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS
Sbjct: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60

Query: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS 114
           TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS
Sbjct: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS 114


>UniRef50_A8ANM7 Putative uncharacterized protein n=11 Tax=Enterobacteriaceae
           RepID=A8ANM7_CITK8
          Length = 118

 Score =  222 bits (565), Expect = 3e-57,   Method: Compositional matrix adjust.
 Identities = 106/113 (93%), Positives = 110/113 (97%)

Query: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60
           MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS
Sbjct: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60

Query: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEA 113
           +LAEPAS+IKTCDHY QFPLYL GE HEHYGIPHGFSSR+ALERYL+GLFGEA
Sbjct: 61  SLAEPASEIKTCDHYHQFPLYLGGEIHEHYGIPHGFSSRIALERYLHGLFGEA 113


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AD55 Uncharacterized protein ygaC n=132 Tax=Enterobac...   219   2e-56
UniRef50_A8ANM7 Putative uncharacterized protein n=11 Tax=Entero...   218   5e-56

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AD55 Uncharacterized protein ygaC n=132 Tax=Enterobacteriaceae
           RepID=YGAC_ECO57
          Length = 114

 Score =  219 bits (559), Expect = 2e-56,   Method: Composition-based stats.
 Identities = 114/114 (100%), Positives = 114/114 (100%)

Query: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60
           MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS
Sbjct: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60

Query: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS 114
           TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS
Sbjct: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEAS 114


>UniRef50_A8ANM7 Putative uncharacterized protein n=11 Tax=Enterobacteriaceae
           RepID=A8ANM7_CITK8
          Length = 118

 Score =  218 bits (555), Expect = 5e-56,   Method: Composition-based stats.
 Identities = 106/113 (93%), Positives = 110/113 (97%)

Query: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60
           MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS
Sbjct: 1   MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARMGRTALVIHPTLKERSS 60

Query: 61  TLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVALERYLNGLFGEA 113
           +LAEPAS+IKTCDHY QFPLYL GE HEHYGIPHGFSSR+ALERYL+GLFGEA
Sbjct: 61  SLAEPASEIKTCDHYHQFPLYLGGEIHEHYGIPHGFSSRIALERYLHGLFGEA 113


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.143    0.443 

Lambda     K      H
   0.267   0.0421    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 455,522,809
Number of Sequences: 3077464
Number of extensions: 16024966
Number of successful extensions: 34418
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 34414
Number of HSP's gapped (non-prelim): 4
length of query: 114
length of database: 1,040,396,356
effective HSP length: 81
effective length of query: 33
effective length of database: 791,121,772
effective search space: 26107018476
effective search space used: 26107018476
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 87 (38.1 bits)