Processing 1 helix: Transmembrane helix 1 from 107 (in) to 122 (out) : 271.061 Score = 0.271061 Processing 1 helix: Transmembrane helix 1 from 107 (out) to 122 (in) : 289.339 Score = 0.289339 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -472.378 Transmembrane helix 2 from 23 (out) to 38 (in) : -239.13 Transmembrane helix 3 from 42 (in) to 57 (out) : -389.328 Transmembrane helix 4 from 61 (out) to 76 (in) : -374.257 Transmembrane helix 5 from 80 (in) to 95 (out) : -392.343 Transmembrane helix 6 from 99 (out) to 114 (in) : 12.919 Transmembrane helix 7 from 118 (in) to 133 (out) : -178.235 Transmembrane helix 8 from 137 (out) to 152 (in) : -317.559 Transmembrane helix 9 from 156 (in) to 171 (out) : -782.268 Transmembrane helix 10 from 176 (out) to 191 (in) : -796.641 Transmembrane helix 11 from 195 (in) to 210 (out) : -140.498 Transmembrane helix 12 from 214 (out) to 229 (in) : -327.788 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -358.246 Transmembrane helix 2 from 31 (in) to 46 (out) : -213.884 Transmembrane helix 3 from 50 (out) to 65 (in) : -418.202 Transmembrane helix 4 from 69 (in) to 84 (out) : -371.03 Transmembrane helix 5 from 88 (out) to 103 (in) : -415.572 Transmembrane helix 6 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 7 from 126 (out) to 141 (in) : -330.958 Transmembrane helix 8 from 145 (in) to 160 (out) : -451.02 Transmembrane helix 9 from 176 (out) to 191 (in) : -796.641 Transmembrane helix 10 from 195 (in) to 210 (out) : -140.498 Transmembrane helix 11 from 214 (out) to 229 (in) : -327.788 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -416.438 Transmembrane helix 2 from 25 (out) to 40 (in) : -231.285 Transmembrane helix 3 from 44 (in) to 59 (out) : -411.158 Transmembrane helix 4 from 63 (out) to 78 (in) : -373.762 Transmembrane helix 5 from 82 (in) to 97 (out) : -384.419 Transmembrane helix 6 from 104 (out) to 119 (in) : 260.462 Transmembrane helix 7 from 123 (in) to 138 (out) : -313.216 Transmembrane helix 8 from 142 (out) to 157 (in) : -412.756 Transmembrane helix 9 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 10 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : -298.113 Transmembrane helix 2 from 35 (in) to 50 (out) : -242.78 Transmembrane helix 3 from 60 (out) to 75 (in) : -375.991 Transmembrane helix 4 from 82 (in) to 97 (out) : -384.419 Transmembrane helix 5 from 104 (out) to 119 (in) : 260.462 Transmembrane helix 6 from 123 (in) to 138 (out) : -313.216 Transmembrane helix 7 from 142 (out) to 157 (in) : -412.756 Transmembrane helix 8 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 9 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -292.987 Transmembrane helix 2 from 35 (out) to 50 (in) : -245.92 Transmembrane helix 3 from 73 (in) to 88 (out) : -359.931 Transmembrane helix 4 from 92 (out) to 107 (in) : -315.346 Transmembrane helix 5 from 111 (in) to 126 (out) : 165.649 Transmembrane helix 6 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 7 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 8 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : -298.113 Transmembrane helix 2 from 35 (in) to 50 (out) : -242.78 Transmembrane helix 3 from 60 (out) to 75 (in) : -375.991 Transmembrane helix 4 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 5 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 6 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 7 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -292.987 Transmembrane helix 2 from 35 (out) to 50 (in) : -245.92 Transmembrane helix 3 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 4 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 5 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 6 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 31 (out) to 46 (in) : -214.916 Transmembrane helix 2 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 3 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 4 from 193 (in) to 208 (out) : -171.896 Transmembrane helix 5 from 212 (out) to 227 (in) : -286.592 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -292.987 Transmembrane helix 2 from 35 (out) to 50 (in) : -245.92 Transmembrane helix 3 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 4 from 197 (out) to 212 (in) : -114.91 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 31 (out) to 46 (in) : -214.916 Transmembrane helix 2 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 3 from 197 (out) to 212 (in) : -114.91 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 2 from 197 (out) to 212 (in) : -114.91 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -484.922 Transmembrane helix 2 from 23 (in) to 38 (out) : -245.37 Transmembrane helix 3 from 42 (out) to 57 (in) : -379.572 Transmembrane helix 4 from 61 (in) to 76 (out) : -380.943 Transmembrane helix 5 from 80 (out) to 95 (in) : -398.157 Transmembrane helix 6 from 99 (in) to 114 (out) : -2.21898 Transmembrane helix 7 from 118 (out) to 133 (in) : -196.365 Transmembrane helix 8 from 137 (in) to 152 (out) : -307.341 Transmembrane helix 9 from 156 (out) to 171 (in) : -788.432 Transmembrane helix 10 from 176 (in) to 191 (out) : -790.059 Transmembrane helix 11 from 195 (out) to 210 (in) : -137.502 Transmembrane helix 12 from 214 (in) to 229 (out) : -322.212 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -416.438 Transmembrane helix 2 from 25 (out) to 40 (in) : -231.285 Transmembrane helix 3 from 44 (in) to 59 (out) : -411.158 Transmembrane helix 4 from 63 (out) to 78 (in) : -373.762 Transmembrane helix 5 from 82 (in) to 97 (out) : -384.419 Transmembrane helix 6 from 104 (out) to 119 (in) : 260.462 Transmembrane helix 7 from 123 (in) to 138 (out) : -313.216 Transmembrane helix 8 from 142 (out) to 157 (in) : -412.756 Transmembrane helix 9 from 176 (in) to 191 (out) : -790.059 Transmembrane helix 10 from 195 (out) to 210 (in) : -137.502 Transmembrane helix 11 from 214 (in) to 229 (out) : -322.212 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -358.246 Transmembrane helix 2 from 31 (in) to 46 (out) : -213.884 Transmembrane helix 3 from 50 (out) to 65 (in) : -418.202 Transmembrane helix 4 from 69 (in) to 84 (out) : -371.03 Transmembrane helix 5 from 88 (out) to 103 (in) : -415.572 Transmembrane helix 6 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 7 from 126 (out) to 141 (in) : -330.958 Transmembrane helix 8 from 145 (in) to 160 (out) : -451.02 Transmembrane helix 9 from 193 (out) to 208 (in) : -163.704 Transmembrane helix 10 from 212 (in) to 227 (out) : -284.708 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -358.554 Transmembrane helix 2 from 31 (out) to 46 (in) : -214.916 Transmembrane helix 3 from 50 (in) to 65 (out) : -425.398 Transmembrane helix 4 from 69 (out) to 84 (in) : -384.47 Transmembrane helix 5 from 88 (in) to 103 (out) : -418.328 Transmembrane helix 6 from 107 (out) to 122 (in) : 289.339 Transmembrane helix 7 from 135 (in) to 150 (out) : -288.799 Transmembrane helix 8 from 193 (out) to 208 (in) : -163.704 Transmembrane helix 9 from 212 (in) to 227 (out) : -284.708 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : -298.113 Transmembrane helix 2 from 35 (in) to 50 (out) : -242.78 Transmembrane helix 3 from 60 (out) to 75 (in) : -375.991 Transmembrane helix 4 from 82 (in) to 97 (out) : -384.419 Transmembrane helix 5 from 106 (out) to 121 (in) : 288.655 Transmembrane helix 6 from 135 (in) to 150 (out) : -288.799 Transmembrane helix 7 from 193 (out) to 208 (in) : -163.704 Transmembrane helix 8 from 212 (in) to 227 (out) : -284.708 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -292.987 Transmembrane helix 2 from 35 (out) to 50 (in) : -245.92 Transmembrane helix 3 from 74 (in) to 89 (out) : -360.31 Transmembrane helix 4 from 106 (out) to 121 (in) : 288.655 Transmembrane helix 5 from 135 (in) to 150 (out) : -288.799 Transmembrane helix 6 from 193 (out) to 208 (in) : -163.704 Transmembrane helix 7 from 212 (in) to 227 (out) : -284.708 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -211.209 Transmembrane helix 2 from 74 (in) to 89 (out) : -360.31 Transmembrane helix 3 from 106 (out) to 121 (in) : 288.655 Transmembrane helix 4 from 135 (in) to 150 (out) : -288.799 Transmembrane helix 5 from 193 (out) to 208 (in) : -163.704 Transmembrane helix 6 from 212 (in) to 227 (out) : -284.708 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -292.987 Transmembrane helix 2 from 35 (out) to 50 (in) : -245.92 Transmembrane helix 3 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 4 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 5 from 197 (in) to 212 (out) : -116.79 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 31 (out) to 46 (in) : -214.916 Transmembrane helix 2 from 107 (in) to 122 (out) : 271.061 Transmembrane helix 3 from 134 (out) to 149 (in) : -295.853 Transmembrane helix 4 from 197 (in) to 212 (out) : -116.79 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -212.691 Transmembrane helix 2 from 107 (out) to 122 (in) : 289.339 Transmembrane helix 3 from 197 (in) to 212 (out) : -116.79 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 107 (out) to 122 (in) : 289.339 Transmembrane helix 2 from 197 (in) to 212 (out) : -116.79 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.271061 1 helix (-) : Score = 0.289339 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 107-122 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.289339