Processing 1 helix: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Score = 2.392944 Processing 1 helix: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Score = 2.136056 Processing 15 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -1765.43 Transmembrane helix 2 from 32 (out) to 47 (in) : 1959.32 Transmembrane helix 3 from 51 (in) to 66 (out) : -395.34 Transmembrane helix 4 from 70 (out) to 85 (in) : -264.47 Transmembrane helix 5 from 89 (in) to 104 (out) : 570.796 Transmembrane helix 6 from 108 (out) to 123 (in) : 679.364 Transmembrane helix 7 from 127 (in) to 142 (out) : 534.708 Transmembrane helix 8 from 146 (out) to 161 (in) : 456.725 Transmembrane helix 9 from 165 (in) to 180 (out) : 359.58 Transmembrane helix 10 from 184 (out) to 199 (in) : -349.642 Transmembrane helix 11 from 203 (in) to 218 (out) : 682.011 Transmembrane helix 12 from 222 (out) to 237 (in) : 177.121 Transmembrane helix 13 from 241 (in) to 256 (out) : -295.19 Transmembrane helix 14 from 260 (out) to 275 (in) : 1289 Transmembrane helix 15 from 279 (in) to 294 (out) : -199.204 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 32 (out) to 47 (in) : 1959.32 Transmembrane helix 2 from 51 (in) to 66 (out) : -395.34 Transmembrane helix 3 from 70 (out) to 85 (in) : -264.47 Transmembrane helix 4 from 89 (in) to 104 (out) : 570.796 Transmembrane helix 5 from 108 (out) to 123 (in) : 679.364 Transmembrane helix 6 from 127 (in) to 142 (out) : 534.708 Transmembrane helix 7 from 146 (out) to 161 (in) : 456.725 Transmembrane helix 8 from 165 (in) to 180 (out) : 359.58 Transmembrane helix 9 from 184 (out) to 199 (in) : -349.642 Transmembrane helix 10 from 203 (in) to 218 (out) : 682.011 Transmembrane helix 11 from 222 (out) to 237 (in) : 177.121 Transmembrane helix 12 from 241 (in) to 256 (out) : -295.19 Transmembrane helix 13 from 260 (out) to 275 (in) : 1289 Transmembrane helix 14 from 279 (in) to 294 (out) : -199.204 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 64 (out) to 79 (in) : -425.214 Transmembrane helix 3 from 83 (in) to 98 (out) : 158.694 Transmembrane helix 4 from 102 (out) to 117 (in) : 1100.9 Transmembrane helix 5 from 121 (in) to 136 (out) : 207.073 Transmembrane helix 6 from 140 (out) to 155 (in) : 525.999 Transmembrane helix 7 from 159 (in) to 174 (out) : 847.438 Transmembrane helix 8 from 178 (out) to 193 (in) : -358.241 Transmembrane helix 9 from 199 (in) to 214 (out) : 341.642 Transmembrane helix 10 from 218 (out) to 233 (in) : 386.645 Transmembrane helix 11 from 237 (in) to 252 (out) : -247.053 Transmembrane helix 12 from 256 (out) to 271 (in) : 818.811 Transmembrane helix 13 from 275 (in) to 290 (out) : 390.749 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 64 (in) to 79 (out) : -435.686 Transmembrane helix 3 from 83 (out) to 98 (in) : 156.306 Transmembrane helix 4 from 102 (in) to 117 (out) : 1092.9 Transmembrane helix 5 from 121 (out) to 136 (in) : 207.727 Transmembrane helix 6 from 140 (in) to 155 (out) : 535.601 Transmembrane helix 7 from 159 (out) to 174 (in) : 857.362 Transmembrane helix 8 from 186 (in) to 201 (out) : -329.889 Transmembrane helix 9 from 205 (out) to 220 (in) : 744.767 Transmembrane helix 10 from 224 (in) to 239 (out) : 153.66 Transmembrane helix 11 from 244 (out) to 259 (in) : -284.139 Transmembrane helix 12 from 263 (in) to 282 (out) : 1631.49 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 79 (out) to 94 (in) : -43.942 Transmembrane helix 3 from 98 (in) to 114 (out) : 1154.82 Transmembrane helix 4 from 118 (out) to 133 (in) : 188.041 Transmembrane helix 5 from 137 (in) to 152 (out) : 741.584 Transmembrane helix 6 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 7 from 186 (in) to 201 (out) : -329.889 Transmembrane helix 8 from 205 (out) to 220 (in) : 744.767 Transmembrane helix 9 from 224 (in) to 239 (out) : 153.66 Transmembrane helix 10 from 244 (out) to 259 (in) : -284.139 Transmembrane helix 11 from 263 (in) to 282 (out) : 1631.49 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 80 (in) to 95 (out) : 0.654001 Transmembrane helix 3 from 99 (out) to 114 (in) : 1119.93 Transmembrane helix 4 from 118 (in) to 133 (out) : 184.759 Transmembrane helix 5 from 137 (out) to 152 (in) : 729.616 Transmembrane helix 6 from 158 (in) to 174 (out) : 858.959 Transmembrane helix 7 from 205 (out) to 220 (in) : 744.767 Transmembrane helix 8 from 224 (in) to 239 (out) : 153.66 Transmembrane helix 9 from 244 (out) to 259 (in) : -284.139 Transmembrane helix 10 from 263 (in) to 282 (out) : 1631.49 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 79 (out) to 94 (in) : -43.942 Transmembrane helix 3 from 98 (in) to 114 (out) : 1154.82 Transmembrane helix 4 from 118 (out) to 133 (in) : 188.041 Transmembrane helix 5 from 137 (in) to 152 (out) : 741.584 Transmembrane helix 6 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 7 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 8 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 9 from 262 (in) to 282 (out) : 1639.89 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 83 (in) to 98 (out) : 158.694 Transmembrane helix 3 from 102 (out) to 119 (in) : 1131.52 Transmembrane helix 4 from 129 (in) to 151 (out) : 984.692 Transmembrane helix 5 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 6 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 7 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 8 from 262 (in) to 282 (out) : 1639.89 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 90 (out) to 119 (in) : 1427.98 Transmembrane helix 3 from 129 (in) to 151 (out) : 984.692 Transmembrane helix 4 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 5 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 6 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 7 from 262 (in) to 282 (out) : 1639.89 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 90 (in) to 119 (out) : 1423.62 Transmembrane helix 3 from 129 (out) to 151 (in) : 965.908 Transmembrane helix 4 from 158 (in) to 174 (out) : 858.959 Transmembrane helix 5 from 206 (out) to 233 (in) : 934.01 Transmembrane helix 6 from 262 (in) to 282 (out) : 1639.89 Score = 7.841834 Processing 5 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 90 (out) to 119 (in) : 1427.98 Transmembrane helix 3 from 141 (in) to 171 (out) : 1251.57 Transmembrane helix 4 from 206 (out) to 233 (in) : 934.01 Transmembrane helix 5 from 262 (in) to 282 (out) : 1639.89 Score = 7.586228 Processing 4 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 90 (in) to 119 (out) : 1423.62 Transmembrane helix 3 from 141 (out) to 171 (in) : 1262.63 Transmembrane helix 4 from 262 (in) to 282 (out) : 1639.89 Score = 6.407805 Processing 3 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 90 (out) to 119 (in) : 1427.98 Transmembrane helix 3 from 262 (in) to 282 (out) : 1639.89 Score = 5.451553 Processing 2 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 262 (in) to 282 (out) : 1639.89 Score = 3.758206 Processing 15 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -1738.47 Transmembrane helix 2 from 32 (in) to 47 (out) : 2002.98 Transmembrane helix 3 from 51 (out) to 66 (in) : -376.46 Transmembrane helix 4 from 70 (in) to 85 (out) : -276.03 Transmembrane helix 5 from 89 (out) to 104 (in) : 566.304 Transmembrane helix 6 from 108 (in) to 123 (out) : 665.036 Transmembrane helix 7 from 127 (out) to 142 (in) : 530.792 Transmembrane helix 8 from 146 (in) to 161 (out) : 456.075 Transmembrane helix 9 from 165 (out) to 180 (in) : 362.82 Transmembrane helix 10 from 184 (in) to 199 (out) : -342.658 Transmembrane helix 11 from 203 (out) to 218 (in) : 664.289 Transmembrane helix 12 from 222 (in) to 237 (out) : 181.879 Transmembrane helix 13 from 241 (out) to 256 (in) : -298.41 Transmembrane helix 14 from 260 (in) to 275 (out) : 1272.8 Transmembrane helix 15 from 279 (out) to 294 (in) : -196.496 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 32 (in) to 47 (out) : 2002.98 Transmembrane helix 2 from 51 (out) to 66 (in) : -376.46 Transmembrane helix 3 from 70 (in) to 85 (out) : -276.03 Transmembrane helix 4 from 89 (out) to 104 (in) : 566.304 Transmembrane helix 5 from 108 (in) to 123 (out) : 665.036 Transmembrane helix 6 from 127 (out) to 142 (in) : 530.792 Transmembrane helix 7 from 146 (in) to 161 (out) : 456.075 Transmembrane helix 8 from 165 (out) to 180 (in) : 362.82 Transmembrane helix 9 from 184 (in) to 199 (out) : -342.658 Transmembrane helix 10 from 203 (out) to 218 (in) : 664.289 Transmembrane helix 11 from 222 (in) to 237 (out) : 181.879 Transmembrane helix 12 from 241 (out) to 256 (in) : -298.41 Transmembrane helix 13 from 260 (in) to 275 (out) : 1272.8 Transmembrane helix 14 from 279 (out) to 294 (in) : -196.496 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 61 (in) to 76 (out) : -504.247 Transmembrane helix 3 from 80 (out) to 95 (in) : -7.054 Transmembrane helix 4 from 99 (in) to 114 (out) : 1117.67 Transmembrane helix 5 from 118 (out) to 133 (in) : 188.041 Transmembrane helix 6 from 137 (in) to 152 (out) : 741.584 Transmembrane helix 7 from 157 (out) to 172 (in) : 817.387 Transmembrane helix 8 from 176 (in) to 191 (out) : -336.325 Transmembrane helix 9 from 195 (out) to 210 (in) : 5.83998 Transmembrane helix 10 from 214 (in) to 229 (out) : 658.104 Transmembrane helix 11 from 233 (out) to 248 (in) : -174.381 Transmembrane helix 12 from 252 (in) to 267 (out) : 296.641 Transmembrane helix 13 from 271 (out) to 286 (in) : 963.873 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 64 (out) to 79 (in) : -425.214 Transmembrane helix 3 from 83 (in) to 98 (out) : 158.694 Transmembrane helix 4 from 102 (out) to 117 (in) : 1100.9 Transmembrane helix 5 from 121 (in) to 136 (out) : 207.073 Transmembrane helix 6 from 140 (out) to 155 (in) : 525.999 Transmembrane helix 7 from 159 (in) to 174 (out) : 847.438 Transmembrane helix 8 from 186 (out) to 201 (in) : -329.211 Transmembrane helix 9 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 10 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 11 from 243 (in) to 258 (out) : -288.4 Transmembrane helix 12 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 80 (in) to 95 (out) : 0.654001 Transmembrane helix 3 from 99 (out) to 114 (in) : 1119.93 Transmembrane helix 4 from 118 (in) to 133 (out) : 184.759 Transmembrane helix 5 from 137 (out) to 152 (in) : 729.616 Transmembrane helix 6 from 158 (in) to 174 (out) : 858.959 Transmembrane helix 7 from 186 (out) to 201 (in) : -329.211 Transmembrane helix 8 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 9 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 10 from 243 (in) to 258 (out) : -288.4 Transmembrane helix 11 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 79 (out) to 94 (in) : -43.942 Transmembrane helix 3 from 98 (in) to 114 (out) : 1154.82 Transmembrane helix 4 from 118 (out) to 133 (in) : 188.041 Transmembrane helix 5 from 137 (in) to 152 (out) : 741.584 Transmembrane helix 6 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 7 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 8 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 9 from 243 (in) to 258 (out) : -288.4 Transmembrane helix 10 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 83 (in) to 98 (out) : 158.694 Transmembrane helix 3 from 102 (out) to 119 (in) : 1131.52 Transmembrane helix 4 from 129 (in) to 151 (out) : 984.692 Transmembrane helix 5 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 6 from 205 (in) to 220 (out) : 765.133 Transmembrane helix 7 from 224 (out) to 239 (in) : 153.54 Transmembrane helix 8 from 243 (in) to 258 (out) : -288.4 Transmembrane helix 9 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 83 (out) to 98 (in) : 156.306 Transmembrane helix 3 from 102 (in) to 119 (out) : 1121.48 Transmembrane helix 4 from 129 (out) to 151 (in) : 965.908 Transmembrane helix 5 from 158 (in) to 174 (out) : 858.959 Transmembrane helix 6 from 205 (out) to 220 (in) : 744.767 Transmembrane helix 7 from 224 (in) to 239 (out) : 153.66 Transmembrane helix 8 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 83 (in) to 98 (out) : 158.694 Transmembrane helix 3 from 102 (out) to 119 (in) : 1131.52 Transmembrane helix 4 from 129 (in) to 151 (out) : 984.692 Transmembrane helix 5 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 6 from 206 (in) to 232 (out) : 972.399 Transmembrane helix 7 from 262 (out) to 282 (in) : 1668.31 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 90 (out) to 119 (in) : 1427.98 Transmembrane helix 3 from 129 (in) to 151 (out) : 984.692 Transmembrane helix 4 from 158 (out) to 174 (in) : 870.641 Transmembrane helix 5 from 206 (in) to 232 (out) : 972.399 Transmembrane helix 6 from 262 (out) to 282 (in) : 1668.31 Score = 8.435352 Processing 5 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 90 (in) to 119 (out) : 1423.62 Transmembrane helix 3 from 141 (out) to 171 (in) : 1262.63 Transmembrane helix 4 from 206 (in) to 232 (out) : 972.399 Transmembrane helix 5 from 262 (out) to 282 (in) : 1668.31 Score = 7.524958 Processing 4 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 90 (out) to 119 (in) : 1427.98 Transmembrane helix 3 from 141 (in) to 171 (out) : 1251.57 Transmembrane helix 4 from 262 (out) to 282 (in) : 1668.31 Score = 6.795195 Processing 3 helices: Transmembrane helix 1 from 33 (out) to 53 (in) : 2231.52 Transmembrane helix 2 from 90 (in) to 119 (out) : 1423.62 Transmembrane helix 3 from 262 (out) to 282 (in) : 1668.31 Score = 5.237248 Processing 2 helices: Transmembrane helix 1 from 33 (in) to 53 (out) : 2297.48 Transmembrane helix 2 from 262 (out) to 282 (in) : 1668.31 Score = 4.078994 Summary of topology analysis: 1 helix (+) : Score = 2.39294 1 helix (-) : Score = 2.13606 2 helices (+) : Score = 4.07899 2 helices (-) : Score = 3.75821 3 helices (+) : Score = 5.45155 3 helices (-) : Score = 5.23725 4 helices (+) : Score = 6.7952 4 helices (-) : Score = 6.40781 5 helices (+) : Score = 7.58623 5 helices (-) : Score = 7.52496 6 helices (+) : Score = 8.43535 6 helices (-) : Score = 7.84183 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.799 Topology: 33-53,90-119,129-151,158-174,206-232,262-282 Re-entrant helices: Not detected. Helix count: 6 N-terminal: in Score: 8.43535