Processing 1 helix: Transmembrane helix 1 from 69 (in) to 90 (out) : 4391.61 Score = 4.391613 Processing 1 helix: Transmembrane helix 1 from 69 (out) to 90 (in) : 4412.79 Score = 4.412787 Processing 18 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -1401.23 Transmembrane helix 2 from 23 (out) to 38 (in) : -8374.98 Transmembrane helix 3 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 4 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 5 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 6 from 99 (out) to 114 (in) : -1134.45 Transmembrane helix 7 from 118 (in) to 133 (out) : -501.729 Transmembrane helix 8 from 137 (out) to 152 (in) : -1002.21 Transmembrane helix 9 from 156 (in) to 171 (out) : -1506.58 Transmembrane helix 10 from 175 (out) to 190 (in) : -1150.72 Transmembrane helix 11 from 194 (in) to 209 (out) : -1484.64 Transmembrane helix 12 from 213 (out) to 228 (in) : -1178.2 Transmembrane helix 13 from 232 (in) to 247 (out) : -416.643 Transmembrane helix 14 from 251 (out) to 266 (in) : -179.59 Transmembrane helix 15 from 270 (in) to 285 (out) : -783.541 Transmembrane helix 16 from 289 (out) to 304 (in) : -889.921 Transmembrane helix 17 from 308 (in) to 323 (out) : -1199.95 Transmembrane helix 18 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -1185.49 Transmembrane helix 2 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 3 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 4 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 5 from 99 (out) to 114 (in) : -1134.45 Transmembrane helix 6 from 118 (in) to 133 (out) : -501.729 Transmembrane helix 7 from 137 (out) to 152 (in) : -1002.21 Transmembrane helix 8 from 156 (in) to 171 (out) : -1506.58 Transmembrane helix 9 from 175 (out) to 190 (in) : -1150.72 Transmembrane helix 10 from 194 (in) to 209 (out) : -1484.64 Transmembrane helix 11 from 213 (out) to 228 (in) : -1178.2 Transmembrane helix 12 from 232 (in) to 247 (out) : -416.643 Transmembrane helix 13 from 251 (out) to 266 (in) : -179.59 Transmembrane helix 14 from 270 (in) to 285 (out) : -783.541 Transmembrane helix 15 from 289 (out) to 304 (in) : -889.921 Transmembrane helix 16 from 308 (in) to 323 (out) : -1199.95 Transmembrane helix 17 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 100 (in) to 115 (out) : -1092.24 Transmembrane helix 6 from 119 (out) to 134 (in) : -524.355 Transmembrane helix 7 from 139 (in) to 154 (out) : -998.05 Transmembrane helix 8 from 159 (out) to 174 (in) : -1474.02 Transmembrane helix 9 from 178 (in) to 193 (out) : -1157.48 Transmembrane helix 10 from 207 (out) to 222 (in) : -1230.01 Transmembrane helix 11 from 226 (in) to 241 (out) : -995.82 Transmembrane helix 12 from 245 (out) to 260 (in) : 293.643 Transmembrane helix 13 from 264 (in) to 279 (out) : -688.974 Transmembrane helix 14 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 15 from 308 (in) to 323 (out) : -1199.95 Transmembrane helix 16 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -1185.49 Transmembrane helix 2 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 3 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 4 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 5 from 100 (out) to 115 (in) : -1110.16 Transmembrane helix 6 from 119 (in) to 134 (out) : -516.245 Transmembrane helix 7 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 8 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 9 from 207 (out) to 222 (in) : -1230.01 Transmembrane helix 10 from 226 (in) to 241 (out) : -995.82 Transmembrane helix 11 from 245 (out) to 260 (in) : 293.643 Transmembrane helix 12 from 264 (in) to 279 (out) : -688.974 Transmembrane helix 13 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 14 from 308 (in) to 323 (out) : -1199.95 Transmembrane helix 15 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 100 (in) to 115 (out) : -1092.24 Transmembrane helix 6 from 119 (out) to 134 (in) : -524.355 Transmembrane helix 7 from 139 (in) to 154 (out) : -998.05 Transmembrane helix 8 from 174 (out) to 189 (in) : -1136.05 Transmembrane helix 9 from 211 (in) to 226 (out) : -1164.58 Transmembrane helix 10 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 11 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 12 from 276 (out) to 291 (in) : -778.762 Transmembrane helix 13 from 295 (in) to 310 (out) : -1160.92 Transmembrane helix 14 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 100 (in) to 115 (out) : -1092.24 Transmembrane helix 5 from 119 (out) to 134 (in) : -524.355 Transmembrane helix 6 from 139 (in) to 154 (out) : -998.05 Transmembrane helix 7 from 174 (out) to 189 (in) : -1136.05 Transmembrane helix 8 from 211 (in) to 226 (out) : -1164.58 Transmembrane helix 9 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 10 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 11 from 276 (out) to 291 (in) : -778.762 Transmembrane helix 12 from 295 (in) to 310 (out) : -1160.92 Transmembrane helix 13 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 6 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 7 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 8 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 9 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 10 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 11 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 12 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 5 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 6 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 7 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 8 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 9 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 10 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 11 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 6 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 7 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 8 from 258 (out) to 273 (in) : -571.435 Transmembrane helix 9 from 279 (in) to 294 (out) : -775.981 Transmembrane helix 10 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 5 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 6 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 7 from 258 (out) to 273 (in) : -571.435 Transmembrane helix 8 from 279 (in) to 294 (out) : -775.981 Transmembrane helix 9 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 4 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 5 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 6 from 258 (out) to 273 (in) : -571.435 Transmembrane helix 7 from 279 (in) to 294 (out) : -775.981 Transmembrane helix 8 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Transmembrane helix 3 from 115 (out) to 130 (in) : -441.146 Transmembrane helix 4 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 5 from 258 (out) to 273 (in) : -571.435 Transmembrane helix 6 from 279 (in) to 294 (out) : -775.981 Transmembrane helix 7 from 327 (out) to 342 (in) : -937.701 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 4 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 5 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 6 from 283 (out) to 298 (in) : -772.188 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Transmembrane helix 3 from 115 (out) to 130 (in) : -441.146 Transmembrane helix 4 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 5 from 258 (out) to 273 (in) : -571.435 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 4 from 241 (out) to 256 (in) : 377.099 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Transmembrane helix 3 from 241 (out) to 256 (in) : 377.099 Score = 6.296701 Processing 2 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Score = 5.958298 Processing 18 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -1410.47 Transmembrane helix 2 from 23 (in) to 38 (out) : -8357.62 Transmembrane helix 3 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 4 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 5 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 6 from 99 (in) to 114 (out) : -1120.85 Transmembrane helix 7 from 118 (out) to 133 (in) : -507.971 Transmembrane helix 8 from 137 (in) to 152 (out) : -1008.99 Transmembrane helix 9 from 156 (out) to 171 (in) : -1496.32 Transmembrane helix 10 from 175 (in) to 190 (out) : -1144.67 Transmembrane helix 11 from 194 (out) to 209 (in) : -1486.06 Transmembrane helix 12 from 213 (in) to 228 (out) : -1177.1 Transmembrane helix 13 from 232 (out) to 247 (in) : -416.257 Transmembrane helix 14 from 251 (in) to 266 (out) : -169.61 Transmembrane helix 15 from 270 (out) to 285 (in) : -783.759 Transmembrane helix 16 from 289 (in) to 304 (out) : -895.879 Transmembrane helix 17 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 18 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 99 (in) to 114 (out) : -1120.85 Transmembrane helix 6 from 118 (out) to 133 (in) : -507.971 Transmembrane helix 7 from 137 (in) to 152 (out) : -1008.99 Transmembrane helix 8 from 156 (out) to 171 (in) : -1496.32 Transmembrane helix 9 from 175 (in) to 190 (out) : -1144.67 Transmembrane helix 10 from 194 (out) to 209 (in) : -1486.06 Transmembrane helix 11 from 213 (in) to 228 (out) : -1177.1 Transmembrane helix 12 from 232 (out) to 247 (in) : -416.257 Transmembrane helix 13 from 251 (in) to 266 (out) : -169.61 Transmembrane helix 14 from 270 (out) to 285 (in) : -783.759 Transmembrane helix 15 from 289 (in) to 304 (out) : -895.879 Transmembrane helix 16 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 17 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -1185.49 Transmembrane helix 2 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 3 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 4 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 5 from 100 (out) to 115 (in) : -1110.16 Transmembrane helix 6 from 119 (in) to 134 (out) : -516.245 Transmembrane helix 7 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 8 from 173 (in) to 188 (out) : -1130.33 Transmembrane helix 9 from 192 (out) to 207 (in) : -1494.84 Transmembrane helix 10 from 211 (in) to 226 (out) : -1164.58 Transmembrane helix 11 from 230 (out) to 245 (in) : -663.561 Transmembrane helix 12 from 249 (in) to 264 (out) : -0.222005 Transmembrane helix 13 from 268 (out) to 283 (in) : -745.826 Transmembrane helix 14 from 287 (in) to 302 (out) : -826.566 Transmembrane helix 15 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 16 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 100 (in) to 115 (out) : -1092.24 Transmembrane helix 6 from 119 (out) to 134 (in) : -524.355 Transmembrane helix 7 from 139 (in) to 154 (out) : -998.05 Transmembrane helix 8 from 174 (out) to 189 (in) : -1136.05 Transmembrane helix 9 from 207 (in) to 222 (out) : -1226.89 Transmembrane helix 10 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 11 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 12 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 13 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 14 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 15 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -1185.49 Transmembrane helix 2 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 3 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 4 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 5 from 100 (out) to 115 (in) : -1110.16 Transmembrane helix 6 from 119 (in) to 134 (out) : -516.245 Transmembrane helix 7 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 8 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 9 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 10 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 11 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 12 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 13 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 14 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 6 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 7 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 8 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 9 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 10 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 11 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 12 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 13 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 5 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 6 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 7 from 226 (out) to 241 (in) : -990.28 Transmembrane helix 8 from 245 (in) to 260 (out) : 297.957 Transmembrane helix 9 from 264 (out) to 279 (in) : -689.226 Transmembrane helix 10 from 283 (in) to 298 (out) : -772.112 Transmembrane helix 11 from 308 (out) to 323 (in) : -1200.85 Transmembrane helix 12 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -1165.81 Transmembrane helix 2 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 3 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 4 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 5 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 6 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 7 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 8 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 9 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 10 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 11 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 5 from 139 (out) to 154 (in) : -990.75 Transmembrane helix 6 from 174 (in) to 189 (out) : -1128.16 Transmembrane helix 7 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 8 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 9 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 10 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 61 (out) to 76 (in) : 1110.3 Transmembrane helix 3 from 80 (in) to 95 (out) : 2306.02 Transmembrane helix 4 from 115 (out) to 130 (in) : -441.146 Transmembrane helix 5 from 139 (in) to 154 (out) : -998.05 Transmembrane helix 6 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 7 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 8 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 9 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 61 (in) to 76 (out) : 1117.3 Transmembrane helix 3 from 80 (out) to 95 (in) : 2313.08 Transmembrane helix 4 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 5 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 6 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 7 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 8 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 4 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 5 from 257 (in) to 272 (out) : -528.143 Transmembrane helix 6 from 283 (out) to 298 (in) : -772.188 Transmembrane helix 7 from 327 (in) to 342 (out) : -927.499 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Transmembrane helix 3 from 115 (out) to 130 (in) : -441.146 Transmembrane helix 4 from 239 (in) to 254 (out) : 328.364 Transmembrane helix 5 from 258 (out) to 273 (in) : -571.435 Transmembrane helix 6 from 279 (in) to 294 (out) : -775.981 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 115 (in) to 130 (out) : -439.554 Transmembrane helix 4 from 238 (out) to 253 (in) : 292.87 Transmembrane helix 5 from 257 (in) to 272 (out) : -528.143 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Transmembrane helix 3 from 115 (out) to 130 (in) : -441.146 Transmembrane helix 4 from 241 (in) to 256 (out) : 375.101 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : 1539.25 Transmembrane helix 2 from 69 (out) to 90 (in) : 4412.79 Transmembrane helix 3 from 241 (in) to 256 (out) : 375.101 Score = 6.333399 Processing 2 helices: Transmembrane helix 1 from 42 (out) to 57 (in) : 1534.25 Transmembrane helix 2 from 69 (in) to 90 (out) : 4391.61 Score = 5.919602 Summary of topology analysis: 1 helix (+) : Score = 4.39161 1 helix (-) : Score = 4.41279 2 helices (+) : Score = 5.9583 2 helices (-) : Score = 5.9196 3 helices (+) : Score = 6.3334 3 helices (-) : Score = 6.2967 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 Results: Signal peptide: 1-37 Signal score: 20.312 Topology: 42-57,69-90,241-256 Re-entrant helices: Not detected. Helix count: 3 N-terminal: out Score: 6.3334