Processing 1 helix: Transmembrane helix 1 from 128 (in) to 143 (out) : -716.981 Score = -0.716976 Processing 1 helix: Transmembrane helix 1 from 128 (out) to 143 (in) : -727.619 Score = -0.727624 Processing 7 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 33 (out) to 48 (in) : -2019.07 Transmembrane helix 3 from 52 (in) to 67 (out) : -2189.67 Transmembrane helix 4 from 71 (out) to 86 (in) : -1717.83 Transmembrane helix 5 from 90 (in) to 105 (out) : -1582.53 Transmembrane helix 6 from 109 (out) to 124 (in) : -1436.16 Transmembrane helix 7 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 33 (in) to 48 (out) : -2024.93 Transmembrane helix 3 from 71 (out) to 86 (in) : -1717.83 Transmembrane helix 4 from 90 (in) to 105 (out) : -1582.53 Transmembrane helix 5 from 109 (out) to 124 (in) : -1436.16 Transmembrane helix 6 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 71 (out) to 86 (in) : -1717.83 Transmembrane helix 3 from 90 (in) to 105 (out) : -1582.53 Transmembrane helix 4 from 109 (out) to 124 (in) : -1436.16 Transmembrane helix 5 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 85 (in) to 100 (out) : -1524.88 Transmembrane helix 3 from 109 (out) to 124 (in) : -1436.16 Transmembrane helix 4 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 109 (out) to 124 (in) : -1436.16 Transmembrane helix 3 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 128 (in) to 143 (out) : -716.981 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 33 (in) to 48 (out) : -2024.93 Transmembrane helix 3 from 52 (out) to 67 (in) : -2182.93 Transmembrane helix 4 from 71 (in) to 86 (out) : -1722.67 Transmembrane helix 5 from 90 (out) to 105 (in) : -1582.27 Transmembrane helix 6 from 109 (in) to 124 (out) : -1432.54 Transmembrane helix 7 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 33 (out) to 48 (in) : -2019.07 Transmembrane helix 3 from 71 (in) to 86 (out) : -1722.67 Transmembrane helix 4 from 90 (out) to 105 (in) : -1582.27 Transmembrane helix 5 from 109 (in) to 124 (out) : -1432.54 Transmembrane helix 6 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 71 (in) to 86 (out) : -1722.67 Transmembrane helix 3 from 90 (out) to 105 (in) : -1582.27 Transmembrane helix 4 from 109 (in) to 124 (out) : -1432.54 Transmembrane helix 5 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 82 (out) to 97 (in) : -1520.48 Transmembrane helix 3 from 109 (in) to 124 (out) : -1432.54 Transmembrane helix 4 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -895.077 Transmembrane helix 2 from 109 (in) to 124 (out) : -1432.54 Transmembrane helix 3 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -908.523 Transmembrane helix 2 from 128 (out) to 143 (in) : -727.619 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.716976 1 helix (-) : Score = -0.727624 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 19.657 Topology: 128-143 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.716976