Processing 1 helix: Transmembrane helix 1 from 123 (in) to 140 (out) : 2914.57 Score = 2.914571 Processing 1 helix: Transmembrane helix 1 from 123 (out) to 141 (in) : 2951.64 Score = 2.951639 Processing 16 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -1312.16 Transmembrane helix 2 from 23 (out) to 38 (in) : 2129.73 Transmembrane helix 3 from 42 (in) to 57 (out) : 191.229 Transmembrane helix 4 from 61 (out) to 76 (in) : 131.564 Transmembrane helix 5 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 6 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 7 from 123 (in) to 138 (out) : 2853.13 Transmembrane helix 8 from 142 (out) to 157 (in) : -592.931 Transmembrane helix 9 from 161 (in) to 176 (out) : 1396.87 Transmembrane helix 10 from 180 (out) to 195 (in) : 1121.16 Transmembrane helix 11 from 199 (in) to 214 (out) : 123.689 Transmembrane helix 12 from 218 (out) to 233 (in) : -906.742 Transmembrane helix 13 from 237 (in) to 252 (out) : -518.479 Transmembrane helix 14 from 256 (out) to 271 (in) : -630.961 Transmembrane helix 15 from 275 (in) to 290 (out) : -699.354 Transmembrane helix 16 from 294 (out) to 309 (in) : -1025.73 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 5 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 6 from 123 (in) to 138 (out) : 2853.13 Transmembrane helix 7 from 142 (out) to 157 (in) : -592.931 Transmembrane helix 8 from 161 (in) to 176 (out) : 1396.87 Transmembrane helix 9 from 180 (out) to 195 (in) : 1121.16 Transmembrane helix 10 from 199 (in) to 214 (out) : 123.689 Transmembrane helix 11 from 218 (out) to 233 (in) : -906.742 Transmembrane helix 12 from 237 (in) to 252 (out) : -518.479 Transmembrane helix 13 from 256 (out) to 271 (in) : -630.961 Transmembrane helix 14 from 275 (in) to 290 (out) : -699.354 Transmembrane helix 15 from 294 (out) to 309 (in) : -1025.73 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 147 (in) to 162 (out) : -254.99 Transmembrane helix 8 from 166 (out) to 181 (in) : 1182.69 Transmembrane helix 9 from 185 (in) to 200 (out) : 1486.09 Transmembrane helix 10 from 204 (out) to 219 (in) : -332.41 Transmembrane helix 11 from 223 (in) to 238 (out) : -888.643 Transmembrane helix 12 from 242 (out) to 257 (in) : -379.894 Transmembrane helix 13 from 261 (in) to 276 (out) : -710.393 Transmembrane helix 14 from 280 (out) to 295 (in) : -831.93 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 5 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 6 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 7 from 147 (out) to 162 (in) : -264.41 Transmembrane helix 8 from 166 (in) to 181 (out) : 1196.81 Transmembrane helix 9 from 185 (out) to 200 (in) : 1504.91 Transmembrane helix 10 from 204 (in) to 219 (out) : -338.69 Transmembrane helix 11 from 236 (out) to 251 (in) : -560.629 Transmembrane helix 12 from 255 (in) to 270 (out) : -598.203 Transmembrane helix 13 from 274 (out) to 289 (in) : -679.72 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 147 (in) to 162 (out) : -254.99 Transmembrane helix 8 from 166 (out) to 181 (in) : 1182.69 Transmembrane helix 9 from 185 (in) to 200 (out) : 1486.09 Transmembrane helix 10 from 204 (out) to 219 (in) : -332.41 Transmembrane helix 11 from 243 (in) to 258 (out) : -371.718 Transmembrane helix 12 from 270 (out) to 285 (in) : -658.412 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 5 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 6 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 7 from 147 (out) to 162 (in) : -264.41 Transmembrane helix 8 from 166 (in) to 181 (out) : 1196.81 Transmembrane helix 9 from 185 (out) to 200 (in) : 1504.91 Transmembrane helix 10 from 204 (in) to 219 (out) : -338.69 Transmembrane helix 11 from 243 (out) to 258 (in) : -360.382 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 8 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 9 from 203 (in) to 218 (out) : -266.974 Transmembrane helix 10 from 243 (out) to 258 (in) : -360.382 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 5 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 6 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 7 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 8 from 203 (in) to 218 (out) : -266.974 Transmembrane helix 9 from 243 (out) to 258 (in) : -360.382 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 48 (out) to 69 (in) : 652.788 Transmembrane helix 3 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 4 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 5 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 6 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 7 from 203 (in) to 218 (out) : -266.974 Transmembrane helix 8 from 243 (out) to 258 (in) : -360.382 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 5 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 6 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 7 from 185 (out) to 205 (in) : 1570.03 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 48 (out) to 69 (in) : 652.788 Transmembrane helix 3 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 4 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 5 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 6 from 185 (out) to 205 (in) : 1570.03 Score = 11.054118 Processing 5 helices: Transmembrane helix 1 from 21 (out) to 51 (in) : 2274.35 Transmembrane helix 2 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 3 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 4 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 5 from 185 (out) to 205 (in) : 1570.03 Score = 10.234181 Processing 4 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 85 (out) to 108 (in) : 1910.02 Transmembrane helix 3 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 4 from 168 (out) to 198 (in) : 2417.39 Score = 9.571089 Processing 3 helices: Transmembrane helix 1 from 21 (out) to 42 (in) : 2274.36 Transmembrane helix 2 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 3 from 168 (out) to 198 (in) : 2417.39 Score = 7.520428 Processing 2 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 123 (out) to 141 (in) : 2951.64 Score = 5.337082 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -1305.54 Transmembrane helix 2 from 23 (in) to 38 (out) : 2173.87 Transmembrane helix 3 from 42 (out) to 57 (in) : 193.171 Transmembrane helix 4 from 61 (in) to 76 (out) : 126.836 Transmembrane helix 5 from 80 (out) to 95 (in) : 578.669 Transmembrane helix 6 from 99 (in) to 114 (out) : 843.866 Transmembrane helix 7 from 123 (out) to 138 (in) : 2885.46 Transmembrane helix 8 from 142 (in) to 157 (out) : -580.569 Transmembrane helix 9 from 161 (out) to 176 (in) : 1376.93 Transmembrane helix 10 from 180 (in) to 195 (out) : 1091.44 Transmembrane helix 11 from 199 (out) to 214 (in) : 116.611 Transmembrane helix 12 from 218 (in) to 233 (out) : -901.758 Transmembrane helix 13 from 237 (out) to 252 (in) : -521.421 Transmembrane helix 14 from 256 (in) to 271 (out) : -627.339 Transmembrane helix 15 from 275 (out) to 290 (in) : -702.846 Transmembrane helix 16 from 294 (in) to 309 (out) : -1002.67 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 138 (in) : 2885.46 Transmembrane helix 7 from 142 (in) to 157 (out) : -580.569 Transmembrane helix 8 from 161 (out) to 176 (in) : 1376.93 Transmembrane helix 9 from 180 (in) to 195 (out) : 1091.44 Transmembrane helix 10 from 199 (out) to 214 (in) : 116.611 Transmembrane helix 11 from 218 (in) to 233 (out) : -901.758 Transmembrane helix 12 from 237 (out) to 252 (in) : -521.421 Transmembrane helix 13 from 256 (in) to 271 (out) : -627.339 Transmembrane helix 14 from 275 (out) to 290 (in) : -702.846 Transmembrane helix 15 from 294 (in) to 309 (out) : -1002.67 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 5 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 6 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 7 from 147 (out) to 162 (in) : -264.41 Transmembrane helix 8 from 166 (in) to 181 (out) : 1196.81 Transmembrane helix 9 from 185 (out) to 200 (in) : 1504.91 Transmembrane helix 10 from 204 (in) to 219 (out) : -338.69 Transmembrane helix 11 from 223 (out) to 238 (in) : -886.057 Transmembrane helix 12 from 242 (in) to 257 (out) : -389.606 Transmembrane helix 13 from 261 (out) to 276 (in) : -710.407 Transmembrane helix 14 from 280 (in) to 295 (out) : -827.07 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 147 (in) to 162 (out) : -254.99 Transmembrane helix 8 from 166 (out) to 181 (in) : 1182.69 Transmembrane helix 9 from 185 (in) to 200 (out) : 1486.09 Transmembrane helix 10 from 204 (out) to 219 (in) : -332.41 Transmembrane helix 11 from 236 (in) to 251 (out) : -555.871 Transmembrane helix 12 from 255 (out) to 270 (in) : -600.997 Transmembrane helix 13 from 274 (in) to 289 (out) : -676.18 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 80 (in) to 95 (out) : 587.931 Transmembrane helix 5 from 99 (out) to 114 (in) : 844.034 Transmembrane helix 6 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 7 from 147 (out) to 162 (in) : -264.41 Transmembrane helix 8 from 166 (in) to 181 (out) : 1196.81 Transmembrane helix 9 from 185 (out) to 200 (in) : 1504.91 Transmembrane helix 10 from 204 (in) to 219 (out) : -338.69 Transmembrane helix 11 from 243 (out) to 258 (in) : -360.382 Transmembrane helix 12 from 270 (in) to 285 (out) : -653.888 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 8 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 9 from 203 (in) to 218 (out) : -266.974 Transmembrane helix 10 from 243 (out) to 258 (in) : -360.382 Transmembrane helix 11 from 270 (in) to 285 (out) : -653.888 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 5 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 6 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 7 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 8 from 203 (in) to 218 (out) : -266.974 Transmembrane helix 9 from 243 (out) to 258 (in) : -360.382 Transmembrane helix 10 from 270 (in) to 285 (out) : -653.888 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : 2022.44 Transmembrane helix 2 from 39 (out) to 54 (in) : 194.57 Transmembrane helix 3 from 58 (in) to 73 (out) : 405.882 Transmembrane helix 4 from 77 (out) to 92 (in) : 152.687 Transmembrane helix 5 from 96 (in) to 111 (out) : 1264.42 Transmembrane helix 6 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 7 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 8 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 9 from 203 (in) to 218 (out) : -266.974 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : 1989.16 Transmembrane helix 2 from 39 (in) to 54 (out) : 186.83 Transmembrane helix 3 from 58 (out) to 73 (in) : 415.818 Transmembrane helix 4 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 5 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 6 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 7 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 8 from 203 (in) to 218 (out) : -266.974 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 48 (out) to 69 (in) : 652.788 Transmembrane helix 3 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 4 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 5 from 159 (in) to 180 (out) : 1500.77 Transmembrane helix 6 from 184 (out) to 199 (in) : 1462.06 Transmembrane helix 7 from 203 (in) to 218 (out) : -266.974 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 21 (out) to 42 (in) : 2274.36 Transmembrane helix 2 from 48 (in) to 69 (out) : 633.012 Transmembrane helix 3 from 85 (out) to 108 (in) : 1910.02 Transmembrane helix 4 from 123 (in) to 141 (out) : 2913.66 Transmembrane helix 5 from 159 (out) to 180 (in) : 1469.63 Transmembrane helix 6 from 185 (in) to 205 (out) : 1555.57 Score = 10.638741 Processing 5 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 48 (out) to 69 (in) : 652.788 Transmembrane helix 3 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 4 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 5 from 168 (in) to 198 (out) : 2389.81 Score = 10.372142 Processing 4 helices: Transmembrane helix 1 from 21 (out) to 51 (in) : 2274.35 Transmembrane helix 2 from 85 (in) to 108 (out) : 1928.98 Transmembrane helix 3 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 4 from 168 (in) to 198 (out) : 2389.81 Score = 9.552205 Processing 3 helices: Transmembrane helix 1 from 21 (in) to 42 (out) : 2330.64 Transmembrane helix 2 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 3 from 168 (in) to 198 (out) : 2389.81 Score = 7.758830 Processing 2 helices: Transmembrane helix 1 from 123 (out) to 140 (in) : 2951.03 Transmembrane helix 2 from 168 (in) to 198 (out) : 2389.81 Score = 5.373387 Summary of topology analysis: 1 helix (+) : Score = 2.91457 1 helix (-) : Score = 2.95164 2 helices (+) : Score = 5.33708 2 helices (-) : Score = 5.37339 3 helices (+) : Score = 7.75883 3 helices (-) : Score = 7.52043 4 helices (+) : Score = 9.57109 4 helices (-) : Score = 9.55221 5 helices (+) : Score = 10.3721 5 helices (-) : Score = 10.2342 6 helices (+) : Score = 11.0541 6 helices (-) : Score = 10.6387 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 21-42,48-69,85-108,123-140,159-180,185-205 Re-entrant helices: Not detected. Helix count: 6 N-terminal: in Score: 11.0541