BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (49 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AAX1 Uncharacterized protein ybhT n=129 Tax=Enterobac...    97   2e-19
UniRef50_D2TPG6 Putative membrane protein n=11 Tax=Enterobacteri...    89   5e-17
UniRef50_Q7N6R6 Similar to unknown protein YbhT of Escherichia c...    57   2e-07
UniRef50_B4EST6 Putative membrane protein n=6 Tax=Enterobacteria...    54   1e-06
UniRef50_C4K5R8 Putative uncharacterized protein n=1 Tax=Candida...    50   2e-05

>UniRef50_P0AAX1 Uncharacterized protein ybhT n=129 Tax=Enterobacteriaceae
          RepID=YBHT_ECO57
          Length = 49

 Score = 96.7 bits (239), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 49/49 (100%), Positives = 49/49 (100%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49
          MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH
Sbjct: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49


>UniRef50_D2TPG6 Putative membrane protein n=11 Tax=Enterobacteriaceae
          RepID=D2TPG6_CITRO
          Length = 83

 Score = 89.0 bits (219), Expect = 5e-17,   Method: Compositional matrix adjust.
 Identities = 43/49 (87%), Positives = 47/49 (95%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49
          MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFN+FSG+G+KD+  QNH
Sbjct: 35 MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNLFSGIGQKDRSRQNH 83


>UniRef50_Q7N6R6 Similar to unknown protein YbhT of Escherichia coli n=3
          Tax=Enterobacteriaceae RepID=Q7N6R6_PHOLL
          Length = 44

 Score = 57.0 bits (136), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 27/44 (61%), Positives = 36/44 (81%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ 44
          M EL+KS+ FA++MVPVV   ILGLIYGLGE+FNI S +G+ ++
Sbjct: 1  MFELIKSMAFALVMVPVVTVGILGLIYGLGELFNIVSRIGRSEK 44


>UniRef50_B4EST6 Putative membrane protein n=6 Tax=Enterobacteriaceae
          RepID=B4EST6_PROMH
          Length = 47

 Score = 54.3 bits (129), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 26/37 (70%), Positives = 33/37 (89%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFS 37
          M ELLKSL FA++MVPVVM +I+G+IYGLGE+FN+ S
Sbjct: 1  MFELLKSLGFALLMVPVVMVLIMGIIYGLGEIFNLIS 37


>UniRef50_C4K5R8 Putative uncharacterized protein n=1 Tax=Candidatus Hamiltonella
          defensa 5AT (Acyrthosiphon pisum) RepID=C4K5R8_HAMD5
          Length = 49

 Score = 50.1 bits (118), Expect = 2e-05,   Method: Compositional matrix adjust.
 Identities = 24/47 (51%), Positives = 33/47 (70%), Gaps = 2/47 (4%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQ 47
          M ELLKS +FA++MVPV++ +IL LIYG G++FN+ S   K D    
Sbjct: 4  MFELLKSFLFALLMVPVMITMILSLIYGFGKIFNLIS--HKNDHSSS 48


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_D2TPG6 Putative membrane protein n=11 Tax=Enterobacteri...    72   7e-12
UniRef50_P0AAX1 Uncharacterized protein ybhT n=129 Tax=Enterobac...    61   9e-09
UniRef50_Q7N6R6 Similar to unknown protein YbhT of Escherichia c...    51   1e-05
UniRef50_B4EST6 Putative membrane protein n=6 Tax=Enterobacteria...    47   2e-04
UniRef50_C4K5R8 Putative uncharacterized protein n=1 Tax=Candida...    44   0.002

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_D2TPG6 Putative membrane protein n=11 Tax=Enterobacteriaceae
          RepID=D2TPG6_CITRO
          Length = 83

 Score = 71.8 bits (175), Expect = 7e-12,   Method: Composition-based stats.
 Identities = 43/49 (87%), Positives = 47/49 (95%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49
          MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFN+FSG+G+KD+  QNH
Sbjct: 35 MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNLFSGIGQKDRSRQNH 83


>UniRef50_P0AAX1 Uncharacterized protein ybhT n=129 Tax=Enterobacteriaceae
          RepID=YBHT_ECO57
          Length = 49

 Score = 61.4 bits (148), Expect = 9e-09,   Method: Composition-based stats.
 Identities = 49/49 (100%), Positives = 49/49 (100%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49
          MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH
Sbjct: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH 49


>UniRef50_Q7N6R6 Similar to unknown protein YbhT of Escherichia coli n=3
          Tax=Enterobacteriaceae RepID=Q7N6R6_PHOLL
          Length = 44

 Score = 51.0 bits (121), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 27/44 (61%), Positives = 36/44 (81%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ 44
          M EL+KS+ FA++MVPVV   ILGLIYGLGE+FNI S +G+ ++
Sbjct: 1  MFELIKSMAFALVMVPVVTVGILGLIYGLGELFNIVSRIGRSEK 44


>UniRef50_B4EST6 Putative membrane protein n=6 Tax=Enterobacteriaceae
          RepID=B4EST6_PROMH
          Length = 47

 Score = 46.8 bits (110), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 26/37 (70%), Positives = 33/37 (89%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFS 37
          M ELLKSL FA++MVPVVM +I+G+IYGLGE+FN+ S
Sbjct: 1  MFELLKSLGFALLMVPVVMVLIMGIIYGLGEIFNLIS 37


>UniRef50_C4K5R8 Putative uncharacterized protein n=1 Tax=Candidatus Hamiltonella
          defensa 5AT (Acyrthosiphon pisum) RepID=C4K5R8_HAMD5
          Length = 49

 Score = 43.7 bits (102), Expect = 0.002,   Method: Composition-based stats.
 Identities = 24/47 (51%), Positives = 33/47 (70%), Gaps = 2/47 (4%)

Query: 1  MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQ 47
          M ELLKS +FA++MVPV++ +IL LIYG G++FN+ S   K D    
Sbjct: 4  MFELLKSFLFALLMVPVMITMILSLIYGFGKIFNLIS--HKNDHSSS 48


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.333    0.154    0.427 

Lambda     K      H
   0.267   0.0468    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 186,382,879
Number of Sequences: 3077464
Number of extensions: 5081165
Number of successful extensions: 34245
Number of sequences better than 1.0e-01: 5
Number of HSP's better than  0.1 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 34235
Number of HSP's gapped (non-prelim): 10
length of query: 49
length of database: 1,040,396,356
effective HSP length: 22
effective length of query: 27
effective length of database: 972,692,148
effective search space: 26262687996
effective search space used: 26262687996
T: 11
A: 40
X1: 16 ( 7.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 88 (38.3 bits)