Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	S	L	N	L	N	K	H	I	S	G	Q	F	N	A	E	L	E	S	I	R	T	Q	V	M	T	M	G	G	M	V	E	Q	Q	L	S	D	A	I	T	A	M	H	N	Q	D	S	D	L	A	K	R	V	I	E	G	D	K	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	N	M	M	E	V	A	I	D	E	A	C	V	R	I	I	A	K	R	Q	P	T	A	S	D	L	R	L	V	M	V	I	S	K	T	I	A	E	L	E	R	I	G	D	V	A	D	K	I	C	R	T	A	L	E	K	F	S	Q	Q
Secondary structure
SS confidence
Disorder																			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	Q	P	L	L	V	S	L	E	S	L	G	R	H	T	I	Q	M	L	H	D	V	L	D	A	F	A	R	M	D	I	D	E	A	V	R	I	Y	R	E	D	K	K	V	D	Q	E	Y	E	G	I	V	R	Q	L	M	T	Y	M	M
Secondary structure
SS confidence
Disorder			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	D	S	R	T	I	P	S	V	L	T	A	L	F	C	A	R	S	I	E	R	I	G	D	R	C	Q	N	I	C	E	F	I	F	Y	Y	V	K	G	Q	D	F	R	H	V	G	G	D	E	L	D	K	L	L	A	G	K	D	S	D
Secondary structure
SS confidence
Disorder																																														?									?	?	?	?	?	?	?
Disorder confidence
		.
Sequence		K
Secondary structure
SS confidence
Disorder		?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1sum chain B Region: 12 - 228 Aligned: 217 Modelled: 217 Confidence: 100.0% Identity: 26% Fold: Spectrin repeat-like Superfamily: PhoU-like Family: PhoU-like Phyre2 PDB 1xwm chain A Region: 14 - 222 Aligned: 209 Modelled: 209 Confidence: 100.0% Identity: 30% Fold: Spectrin repeat-like Superfamily: PhoU-like Family: PhoU-like Phyre2 PDB 1t72 chain A Region: 11 - 220 Aligned: 210 Modelled: 209 Confidence: 100.0% Identity: 31% Fold: Spectrin repeat-like Superfamily: PhoU-like Family: PhoU-like Phyre2 PDB 2i0m chain A Region: 13 - 223 Aligned: 206 Modelled: 211 Confidence: 100.0% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:phosphate transport system protein phou; PDBTitle: crystal structure of the phosphate transport system regulatory protein2 phou from streptococcus pneumoniae Phyre2 PDB 3l39 chain A Region: 23 - 217 Aligned: 188 Modelled: 195 Confidence: 99.9% Identity: 8% PDB header:phosphate-binding protein Chain: A: PDB Molecule:putative phou-like phosphate regulatory protein; PDBTitle: crystal structure of putative phou-like phosphate regulatory2 protein (bt4638) from bacteroides thetaiotaomicron vpi-54823 at 1.93 a resolution Phyre2 PDB 2olt chain B Region: 23 - 217 Aligned: 186 Modelled: 186 Confidence: 99.8% Identity: 13% PDB header:unknown function Chain: B: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a phou-like protein (so_3770) from shewanella2 oneidensis mr-1 at 2.00 a resolution Phyre2 PDB 1vct chain A domain 1 Region: 121 - 214 Aligned: 93 Modelled: 94 Confidence: 99.7% Identity: 12% Fold: Spectrin repeat-like Superfamily: PhoU-like Family: PhoU-like Phyre2 PDB 2bkn chain A Region: 21 - 121 Aligned: 97 Modelled: 101 Confidence: 99.3% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:hypothetical protein ph0236; PDBTitle: structure analysis of unknown function protein Phyre2 PDB 1col chain A Region: 200 - 240 Aligned: 39 Modelled: 41 Confidence: 51.2% Identity: 26% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1rh1 chain A domain 2 Region: 200 - 240 Aligned: 39 Modelled: 41 Confidence: 36.0% Identity: 18% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 2qrx chain A Region: 6 - 169 Aligned: 164 Modelled: 164 Confidence: 27.9% Identity: 9% PDB header:dna binding protein Chain: A: PDB Molecule:gm27569p; PDBTitle: crystal structure of drosophila melanogaster translin2 protein Phyre2 PDB 1zke chain A domain 1 Region: 3 - 31 Aligned: 29 Modelled: 29 Confidence: 26.6% Identity: 14% Fold: ROP-like Superfamily: HP1531-like Family: HP1531-like Phyre2 PDB 3qd8 chain M Region: 62 - 213 Aligned: 143 Modelled: 152 Confidence: 25.9% Identity: 17% PDB header:metal binding protein Chain: M: PDB Molecule:probable bacterioferritin bfrb; PDBTitle: crystal structure of mycobacterium tuberculosis bfrb Phyre2 PDB 1pq1 chain B Region: 93 - 104 Aligned: 12 Modelled: 12 Confidence: 22.0% Identity: 50% PDB header:apoptosis Chain: B: PDB Molecule:bcl2-like protein 11; PDBTitle: crystal structure of bcl-xl/bim Phyre2 PDB 1eq1 chain A Region: 10 - 177 Aligned: 150 Modelled: 154 Confidence: 19.3% Identity: 7% Fold: Apolipophorin-III Superfamily: Apolipophorin-III Family: Apolipophorin-III Phyre2 PDB 3izc chain W Region: 66 - 109 Aligned: 44 Modelled: 44 Confidence: 17.6% Identity: 14% PDB header:ribosome Chain: W: PDB Molecule:60s ribosomal protein rpl22 (l22e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome Phyre2 PDB 1jqs chain B Region: 226 - 238 Aligned: 13 Modelled: 13 Confidence: 16.9% Identity: 38% PDB header:ribosome Chain: B: PDB Molecule:elongation factor g; PDBTitle: fitting of l11 protein and elongation factor g (domain g'2 and v) in the cryo-em map of e. coli 70s ribosome bound3 with ef-g and gmppcp, a nonhydrolysable gtp analog Phyre2 PDB 3ci9 chain B Region: 128 - 171 Aligned: 44 Modelled: 44 Confidence: 16.4% Identity: 18% PDB header:transcription Chain: B: PDB Molecule:heat shock factor-binding protein 1; PDBTitle: crystal structure of the human hsbp1 Phyre2 PDB 1set chain A domain 1 Region: 11 - 82 Aligned: 72 Modelled: 72 Confidence: 15.9% Identity: 10% Fold: Long alpha-hairpin Superfamily: tRNA-binding arm Family: Seryl-tRNA synthetase (SerRS) Phyre2 PDB 2k7w chain B Region: 95 - 104 Aligned: 10 Modelled: 10 Confidence: 13.6% Identity: 60% PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-like protein 11; PDBTitle: bax activation is initiated at a novel interaction site Phyre2 1 d1sumb_ 2 d1xwma_ 3 d1t72a_ 4 c2i0mA_ 5 c3l39A_ 6 c2oltB_ 7 d1vcta1 8 c2bknA_ 9 d1cola_ 10 d1rh1a2 11 c2qrxA_ 12 d1zkea1 13 c3qd8M_ 14 c1pq1B_ 15 d1eq1a_ 16 c3izcw_ 17 c1jqsB_ 18 c3ci9B_ 19 d1seta1 20 c2k7wB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1sumb_ 100.0 26 Fold:Spectrin repeat-like Superfamily:PhoU-like Family:PhoU-like 2 d1xwma_ 100.0 30 Fold:Spectrin repeat-like Superfamily:PhoU-like Family:PhoU-like 3 d1t72a_ 100.0 31 Fold:Spectrin repeat-like Superfamily:PhoU-like Family:PhoU-like 4 c2i0mA_ 100.0 31 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:phosphate transport system protein phou; PDBTitle: crystal structure of the phosphate transport system regulatory protein2 phou from streptococcus pneumoniae 5 c3l39A_ 99.9 8 PDB header:phosphate-binding protein Chain: A: PDB Molecule:putative phou-like phosphate regulatory protein; PDBTitle: crystal structure of putative phou-like phosphate regulatory2 protein (bt4638) from bacteroides thetaiotaomicron vpi-54823 at 1.93 a resolution 6 c2oltB_ 99.8 13 PDB header:unknown function Chain: B: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a phou-like protein (so_3770) from shewanella2 oneidensis mr-1 at 2.00 a resolution 7 d1vcta1 99.7 12 Fold:Spectrin repeat-like Superfamily:PhoU-like Family:PhoU-like 8 c2bknA_ 99.3 15 PDB header:membrane protein Chain: A: PDB Molecule:hypothetical protein ph0236; PDBTitle: structure analysis of unknown function protein 9 d1cola_ 51.2 26 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 10 d1rh1a2 36.0 18 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 11 c2qrxA_ 27.9 9 PDB header:dna binding protein Chain: A: PDB Molecule:gm27569p; PDBTitle: crystal structure of drosophila melanogaster translin2 protein 12 d1zkea1 26.6 14 Fold:ROP-like Superfamily:HP1531-like Family:HP1531-like 13 c3qd8M_ 25.9 17 PDB header:metal binding protein Chain: M: PDB Molecule:probable bacterioferritin bfrb; PDBTitle: crystal structure of mycobacterium tuberculosis bfrb 14 c1pq1B_ 22.0 50 PDB header:apoptosis Chain: B: PDB Molecule:bcl2-like protein 11; PDBTitle: crystal structure of bcl-xl/bim 15 d1eq1a_ 19.3 7 Fold:Apolipophorin-III Superfamily:Apolipophorin-III Family:Apolipophorin-III 16 c3izcw_ 17.6 14 PDB header:ribosome Chain: W: PDB Molecule:60s ribosomal protein rpl22 (l22e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome 17 c1jqsB_ 16.9 38 PDB header:ribosome Chain: B: PDB Molecule:elongation factor g; PDBTitle: fitting of l11 protein and elongation factor g (domain g'2 and v) in the cryo-em map of e. coli 70s ribosome bound3 with ef-g and gmppcp, a nonhydrolysable gtp analog 18 c3ci9B_ 16.4 18 PDB header:transcription Chain: B: PDB Molecule:heat shock factor-binding protein 1; PDBTitle: crystal structure of the human hsbp1 19 d1seta1 15.9 10 Fold:Long alpha-hairpin Superfamily:tRNA-binding arm Family:Seryl-tRNA synthetase (SerRS) 20 c2k7wB_ 13.6 60 PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-like protein 11; PDBTitle: bax activation is initiated at a novel interaction site 21 d1krqa_ not modelled 13.6 12 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 22 c2nl9B_ not modelled 11.7 50 PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-like protein 11; PDBTitle: crystal structure of the mcl-1:bim bh3 complex 23 c3kj2B_ not modelled 11.3 50 PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-like protein 11; PDBTitle: mcl-1 in complex with bim bh3 mutant f4ae 24 c1kmiZ_ not modelled 11.2 7 PDB header:signaling protein Chain: Z: PDB Molecule:chemotaxis protein chez; PDBTitle: crystal structure of an e.coli chemotaxis protein, chez 25 c1rimA_ not modelled 10.9 46 PDB header:de novo protein Chain: A: PDB Molecule:e6apc2 peptide; PDBTitle: e6-binding zinc finger (e6apc2) 26 c2kdtA_ not modelled 10.8 15 PDB header:protein transport Chain: A: PDB Molecule:neuroendocrine convertase 1; PDBTitle: pc1/3 dcsg sorting domain structure in dpc 27 c3d1dC_ not modelled 9.3 17 PDB header:nuclear protein Chain: C: PDB Molecule:rna-induced transcriptional silencing complex PDBTitle: hexagonal crystal structure of tas3 c-terminal alpha motif 28 d1vlga_ not modelled 9.0 9 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 29 c3c98B_ not modelled 8.8 14 PDB header:endocytosis/exocytosis Chain: B: PDB Molecule:syntaxin-1a; PDBTitle: revised structure of the munc18a-syntaxin1 complex 30 d1jgca_ not modelled 8.8 17 Fold:Ferritin-like Superfamily:Ferritin-like Family:Ferritin 31 c3kz0C_ not modelled 7.9 42 PDB header:apoptosis Chain: C: PDB Molecule:mcl-1 specific peptide mb7; PDBTitle: mcl-1 complex with mcl-1-specific selected peptide 32 c3kz0D_ not modelled 7.9 42 PDB header:apoptosis Chain: D: PDB Molecule:mcl-1 specific peptide mb7; PDBTitle: mcl-1 complex with mcl-1-specific selected peptide 33 c3d7vB_ not modelled 7.8 43 PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-like protein 11; PDBTitle: crystal structure of mcl-1 in complex with an mcl-12 selective bh3 ligand 34 c3iz5w_ not modelled 6.1 14 PDB header:ribosome Chain: W: PDB Molecule:60s ribosomal protein l22 (l22e); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome 35 d2dmda1 not modelled 6.0 43 Fold:beta-beta-alpha zinc fingers Superfamily:beta-beta-alpha zinc fingers Family:Classic zinc finger, C2H2 36 d1a87a_ not modelled 6.0 20 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 37 c1a87A_ not modelled 6.0 20 PDB header:bacteriocin Chain: A: PDB Molecule:colicin n; PDBTitle: colicin n 38 d1j0ta_ not modelled 6.0 27 Fold:Crustacean CHH/MIH/GIH neurohormone Superfamily:Crustacean CHH/MIH/GIH neurohormone Family:Crustacean CHH/MIH/GIH neurohormone 39 d1uptb_ not modelled 6.0 14 Fold:GRIP domain Superfamily:GRIP domain Family:GRIP domain 40 c3cd0B_ not modelled 5.7 32 PDB header:oxidoreductase Chain: B: PDB Molecule:3-hydroxy-3-methylglutaryl-coenzyme a reductase; PDBTitle: thermodynamic and structure guided design of statin hmg-coa2 reductase inhibitors 41 c1vh6A_ not modelled 5.5 8 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:flagellar protein flis; PDBTitle: crystal structure of a flagellar protein 42 d1vh6a_ not modelled 5.5 8 Fold:Four-helical up-and-down bundle Superfamily:Flagellar export chaperone FliS Family:Flagellar export chaperone FliS 43 c3znfA_ not modelled 5.4 44 PDB header:zinc finger dna binding domain Chain: A: PDB Molecule:zinc finger; PDBTitle: high-resolution three-dimensional structure of a single2 zinc finger from a human enhancer binding protein in3 solution 44 d4znfa_ not modelled 5.4 44 Fold:beta-beta-alpha zinc fingers Superfamily:beta-beta-alpha zinc fingers Family:Classic zinc finger, C2H2 45 c4znfA_ not modelled 5.4 44 PDB header:zinc finger dna binding domain Chain: A: PDB Molecule:zinc finger; PDBTitle: high-resolution three-dimensional structure of a single2 zinc finger from a human enhancer binding protein in3 solution 46 d256ba_ not modelled 5.3 14 Fold:Four-helical up-and-down bundle Superfamily:Cytochromes Family:Cytochrome b562 47 d1t6ua_ not modelled 5.3 11 Fold:Four-helical up-and-down bundle Superfamily:Nickel-containing superoxide dismutase, NiSOD Family:Nickel-containing superoxide dismutase, NiSOD 48 c1hwjB_ not modelled 5.2 32 PDB header:oxidoreductase Chain: B: PDB Molecule:hmg-coa reductase; PDBTitle: complex of the catalytic portion of human hmg-coa reductase2 with cerivastatin 49 d1hw8c2 not modelled 5.2 36 Fold:Substrate-binding domain of HMG-CoA reductase Superfamily:Substrate-binding domain of HMG-CoA reductase Family:Substrate-binding domain of HMG-CoA reductase 50 c3qauA_ not modelled 5.1 27 PDB header:oxidoreductase Chain: A: PDB Molecule:3-hydroxy-3-methylglutaryl-coenzyme a reductase; PDBTitle: 3-hydroxy-3-methylglutaryl-coenzyme a reductase from streptococcus2 pneumoniae 51 d1o9da_ not modelled 5.1 15 Fold:alpha-alpha superhelix Superfamily:14-3-3 protein Family:14-3-3 protein

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0