Processing 1 helix: Transmembrane helix 1 from 228 (in) to 252 (out) : 868.685 Score = 0.868685 Processing 1 helix: Transmembrane helix 1 from 228 (out) to 252 (in) : 861.715 Score = 0.861715 Processing 28 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -1339.09 Transmembrane helix 2 from 23 (out) to 38 (in) : -765.293 Transmembrane helix 3 from 42 (in) to 57 (out) : -343.244 Transmembrane helix 4 from 61 (out) to 76 (in) : -184.481 Transmembrane helix 5 from 80 (in) to 95 (out) : 283.795 Transmembrane helix 6 from 99 (out) to 114 (in) : 16.95 Transmembrane helix 7 from 118 (in) to 133 (out) : -217.369 Transmembrane helix 8 from 137 (out) to 152 (in) : -636.403 Transmembrane helix 9 from 156 (in) to 171 (out) : -1435.24 Transmembrane helix 10 from 175 (out) to 190 (in) : -512.877 Transmembrane helix 11 from 194 (in) to 209 (out) : -162.459 Transmembrane helix 12 from 213 (out) to 228 (in) : -345.946 Transmembrane helix 13 from 232 (in) to 247 (out) : 643.083 Transmembrane helix 14 from 251 (out) to 266 (in) : -177.725 Transmembrane helix 15 from 270 (in) to 285 (out) : 113.572 Transmembrane helix 16 from 289 (out) to 304 (in) : -566.968 Transmembrane helix 17 from 308 (in) to 323 (out) : -257.421 Transmembrane helix 18 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 19 from 346 (in) to 361 (out) : -401.539 Transmembrane helix 20 from 365 (out) to 380 (in) : -305.257 Transmembrane helix 21 from 384 (in) to 399 (out) : -124.51 Transmembrane helix 22 from 403 (out) to 418 (in) : -72.636 Transmembrane helix 23 from 422 (in) to 437 (out) : -42.828 Transmembrane helix 24 from 441 (out) to 456 (in) : -53.63 Transmembrane helix 25 from 460 (in) to 475 (out) : -99.029 Transmembrane helix 26 from 479 (out) to 494 (in) : -239.896 Transmembrane helix 27 from 498 (in) to 513 (out) : -140.949 Transmembrane helix 28 from 517 (out) to 532 (in) : -249.23 Score = -100000.000000 Processing 27 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : -1083.64 Transmembrane helix 2 from 34 (in) to 49 (out) : -486.544 Transmembrane helix 3 from 53 (out) to 68 (in) : -208.219 Transmembrane helix 4 from 72 (in) to 87 (out) : -18.562 Transmembrane helix 5 from 91 (out) to 106 (in) : 427.224 Transmembrane helix 6 from 110 (in) to 125 (out) : -94.549 Transmembrane helix 7 from 129 (out) to 144 (in) : -605.983 Transmembrane helix 8 from 148 (in) to 163 (out) : -970.751 Transmembrane helix 9 from 175 (out) to 190 (in) : -512.877 Transmembrane helix 10 from 194 (in) to 209 (out) : -162.459 Transmembrane helix 11 from 213 (out) to 228 (in) : -345.946 Transmembrane helix 12 from 232 (in) to 247 (out) : 643.083 Transmembrane helix 13 from 251 (out) to 266 (in) : -177.725 Transmembrane helix 14 from 270 (in) to 285 (out) : 113.572 Transmembrane helix 15 from 289 (out) to 304 (in) : -566.968 Transmembrane helix 16 from 308 (in) to 323 (out) : -257.421 Transmembrane helix 17 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 18 from 346 (in) to 361 (out) : -401.539 Transmembrane helix 19 from 365 (out) to 380 (in) : -305.257 Transmembrane helix 20 from 384 (in) to 399 (out) : -124.51 Transmembrane helix 21 from 403 (out) to 418 (in) : -72.636 Transmembrane helix 22 from 422 (in) to 437 (out) : -42.828 Transmembrane helix 23 from 441 (out) to 456 (in) : -53.63 Transmembrane helix 24 from 460 (in) to 475 (out) : -99.029 Transmembrane helix 25 from 479 (out) to 494 (in) : -239.896 Transmembrane helix 26 from 498 (in) to 513 (out) : -140.949 Transmembrane helix 27 from 517 (out) to 532 (in) : -249.23 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -603.864 Transmembrane helix 2 from 47 (out) to 62 (in) : -273.341 Transmembrane helix 3 from 66 (in) to 81 (out) : -127.656 Transmembrane helix 4 from 85 (out) to 100 (in) : 473.682 Transmembrane helix 5 from 104 (in) to 119 (out) : -133.099 Transmembrane helix 6 from 123 (out) to 138 (in) : -375.839 Transmembrane helix 7 from 142 (in) to 157 (out) : -774.366 Transmembrane helix 8 from 175 (out) to 190 (in) : -512.877 Transmembrane helix 9 from 194 (in) to 209 (out) : -162.459 Transmembrane helix 10 from 213 (out) to 228 (in) : -345.946 Transmembrane helix 11 from 232 (in) to 247 (out) : 643.083 Transmembrane helix 12 from 251 (out) to 266 (in) : -177.725 Transmembrane helix 13 from 270 (in) to 285 (out) : 113.572 Transmembrane helix 14 from 289 (out) to 304 (in) : -566.968 Transmembrane helix 15 from 308 (in) to 323 (out) : -257.421 Transmembrane helix 16 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 17 from 346 (in) to 361 (out) : -401.539 Transmembrane helix 18 from 365 (out) to 380 (in) : -305.257 Transmembrane helix 19 from 384 (in) to 399 (out) : -124.51 Transmembrane helix 20 from 403 (out) to 418 (in) : -72.636 Transmembrane helix 21 from 422 (in) to 437 (out) : -42.828 Transmembrane helix 22 from 441 (out) to 456 (in) : -53.63 Transmembrane helix 23 from 460 (in) to 475 (out) : -99.029 Transmembrane helix 24 from 479 (out) to 494 (in) : -239.896 Transmembrane helix 25 from 498 (in) to 513 (out) : -140.949 Transmembrane helix 26 from 517 (out) to 532 (in) : -249.23 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -592.336 Transmembrane helix 2 from 47 (in) to 62 (out) : -265.359 Transmembrane helix 3 from 66 (out) to 81 (in) : -144.144 Transmembrane helix 4 from 85 (in) to 100 (out) : 461.118 Transmembrane helix 5 from 104 (out) to 119 (in) : -129.001 Transmembrane helix 6 from 123 (in) to 138 (out) : -375.261 Transmembrane helix 7 from 142 (out) to 157 (in) : -766.334 Transmembrane helix 8 from 180 (in) to 195 (out) : -230.834 Transmembrane helix 9 from 199 (out) to 214 (in) : -235.771 Transmembrane helix 10 from 218 (in) to 233 (out) : 43.232 Transmembrane helix 11 from 237 (out) to 252 (in) : 462.243 Transmembrane helix 12 from 256 (in) to 271 (out) : -257.425 Transmembrane helix 13 from 275 (out) to 290 (in) : 58.339 Transmembrane helix 14 from 302 (in) to 317 (out) : -189.376 Transmembrane helix 15 from 321 (out) to 336 (in) : -50.096 Transmembrane helix 16 from 340 (in) to 355 (out) : -353.732 Transmembrane helix 17 from 359 (out) to 374 (in) : -338.629 Transmembrane helix 18 from 378 (in) to 393 (out) : -134.631 Transmembrane helix 19 from 397 (out) to 412 (in) : -106.108 Transmembrane helix 20 from 416 (in) to 431 (out) : -38.524 Transmembrane helix 21 from 435 (out) to 450 (in) : -46.268 Transmembrane helix 22 from 454 (in) to 469 (out) : -80.408 Transmembrane helix 23 from 473 (out) to 488 (in) : -209.569 Transmembrane helix 24 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 25 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -465.017 Transmembrane helix 2 from 54 (out) to 69 (in) : -204.864 Transmembrane helix 3 from 73 (in) to 88 (out) : 13.549 Transmembrane helix 4 from 92 (out) to 107 (in) : 380.592 Transmembrane helix 5 from 111 (in) to 126 (out) : -88.171 Transmembrane helix 6 from 130 (out) to 145 (in) : -616.94 Transmembrane helix 7 from 180 (in) to 195 (out) : -230.834 Transmembrane helix 8 from 199 (out) to 214 (in) : -235.771 Transmembrane helix 9 from 218 (in) to 233 (out) : 43.232 Transmembrane helix 10 from 237 (out) to 252 (in) : 462.243 Transmembrane helix 11 from 256 (in) to 271 (out) : -257.425 Transmembrane helix 12 from 275 (out) to 290 (in) : 58.339 Transmembrane helix 13 from 302 (in) to 317 (out) : -189.376 Transmembrane helix 14 from 321 (out) to 336 (in) : -50.096 Transmembrane helix 15 from 340 (in) to 355 (out) : -353.732 Transmembrane helix 16 from 359 (out) to 374 (in) : -338.629 Transmembrane helix 17 from 378 (in) to 393 (out) : -134.631 Transmembrane helix 18 from 397 (out) to 412 (in) : -106.108 Transmembrane helix 19 from 416 (in) to 431 (out) : -38.524 Transmembrane helix 20 from 435 (out) to 450 (in) : -46.268 Transmembrane helix 21 from 454 (in) to 469 (out) : -80.408 Transmembrane helix 22 from 473 (out) to 488 (in) : -209.569 Transmembrane helix 23 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 24 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 36 (out) to 51 (in) : -442.719 Transmembrane helix 2 from 55 (in) to 70 (out) : -200.306 Transmembrane helix 3 from 74 (out) to 89 (in) : 34.167 Transmembrane helix 4 from 93 (in) to 108 (out) : 328.738 Transmembrane helix 5 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 6 from 180 (in) to 195 (out) : -230.834 Transmembrane helix 7 from 199 (out) to 214 (in) : -235.771 Transmembrane helix 8 from 218 (in) to 233 (out) : 43.232 Transmembrane helix 9 from 237 (out) to 252 (in) : 462.243 Transmembrane helix 10 from 256 (in) to 271 (out) : -257.425 Transmembrane helix 11 from 275 (out) to 290 (in) : 58.339 Transmembrane helix 12 from 302 (in) to 317 (out) : -189.376 Transmembrane helix 13 from 321 (out) to 336 (in) : -50.096 Transmembrane helix 14 from 340 (in) to 355 (out) : -353.732 Transmembrane helix 15 from 359 (out) to 374 (in) : -338.629 Transmembrane helix 16 from 378 (in) to 393 (out) : -134.631 Transmembrane helix 17 from 397 (out) to 412 (in) : -106.108 Transmembrane helix 18 from 416 (in) to 431 (out) : -38.524 Transmembrane helix 19 from 435 (out) to 450 (in) : -46.268 Transmembrane helix 20 from 454 (in) to 469 (out) : -80.408 Transmembrane helix 21 from 473 (out) to 488 (in) : -209.569 Transmembrane helix 22 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 23 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 180 (in) to 195 (out) : -230.834 Transmembrane helix 6 from 199 (out) to 214 (in) : -235.771 Transmembrane helix 7 from 218 (in) to 233 (out) : 43.232 Transmembrane helix 8 from 237 (out) to 252 (in) : 462.243 Transmembrane helix 9 from 256 (in) to 271 (out) : -257.425 Transmembrane helix 10 from 275 (out) to 290 (in) : 58.339 Transmembrane helix 11 from 302 (in) to 317 (out) : -189.376 Transmembrane helix 12 from 321 (out) to 336 (in) : -50.096 Transmembrane helix 13 from 340 (in) to 355 (out) : -353.732 Transmembrane helix 14 from 359 (out) to 374 (in) : -338.629 Transmembrane helix 15 from 378 (in) to 393 (out) : -134.631 Transmembrane helix 16 from 397 (out) to 412 (in) : -106.108 Transmembrane helix 17 from 416 (in) to 431 (out) : -38.524 Transmembrane helix 18 from 435 (out) to 450 (in) : -46.268 Transmembrane helix 19 from 454 (in) to 469 (out) : -80.408 Transmembrane helix 20 from 473 (out) to 488 (in) : -209.569 Transmembrane helix 21 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 22 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 36 (out) to 51 (in) : -442.719 Transmembrane helix 2 from 55 (in) to 70 (out) : -200.306 Transmembrane helix 3 from 74 (out) to 89 (in) : 34.167 Transmembrane helix 4 from 93 (in) to 108 (out) : 328.738 Transmembrane helix 5 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 6 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 7 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 8 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 9 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 10 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 11 from 303 (out) to 318 (in) : -202.592 Transmembrane helix 12 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 13 from 357 (out) to 372 (in) : -334.529 Transmembrane helix 14 from 376 (in) to 391 (out) : -155.734 Transmembrane helix 15 from 395 (out) to 410 (in) : -112.07 Transmembrane helix 16 from 414 (in) to 429 (out) : -40.546 Transmembrane helix 17 from 433 (out) to 448 (in) : -45.362 Transmembrane helix 18 from 452 (in) to 467 (out) : -74.871 Transmembrane helix 19 from 471 (out) to 486 (in) : -187.395 Transmembrane helix 20 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 21 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 6 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 7 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 8 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 9 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 10 from 303 (out) to 318 (in) : -202.592 Transmembrane helix 11 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 12 from 357 (out) to 372 (in) : -334.529 Transmembrane helix 13 from 376 (in) to 391 (out) : -155.734 Transmembrane helix 14 from 395 (out) to 410 (in) : -112.07 Transmembrane helix 15 from 414 (in) to 429 (out) : -40.546 Transmembrane helix 16 from 433 (out) to 448 (in) : -45.362 Transmembrane helix 17 from 452 (in) to 467 (out) : -74.871 Transmembrane helix 18 from 471 (out) to 486 (in) : -187.395 Transmembrane helix 19 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 20 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 5 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 6 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 7 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 8 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 9 from 303 (out) to 318 (in) : -202.592 Transmembrane helix 10 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 11 from 357 (out) to 372 (in) : -334.529 Transmembrane helix 12 from 376 (in) to 391 (out) : -155.734 Transmembrane helix 13 from 395 (out) to 410 (in) : -112.07 Transmembrane helix 14 from 414 (in) to 429 (out) : -40.546 Transmembrane helix 15 from 433 (out) to 448 (in) : -45.362 Transmembrane helix 16 from 452 (in) to 467 (out) : -74.871 Transmembrane helix 17 from 471 (out) to 486 (in) : -187.395 Transmembrane helix 18 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 19 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 10 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 11 from 376 (in) to 391 (out) : -155.734 Transmembrane helix 12 from 395 (out) to 410 (in) : -112.07 Transmembrane helix 13 from 414 (in) to 429 (out) : -40.546 Transmembrane helix 14 from 433 (out) to 448 (in) : -45.362 Transmembrane helix 15 from 452 (in) to 467 (out) : -74.871 Transmembrane helix 16 from 471 (out) to 486 (in) : -187.395 Transmembrane helix 17 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 18 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 9 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 10 from 376 (in) to 391 (out) : -155.734 Transmembrane helix 11 from 395 (out) to 410 (in) : -112.07 Transmembrane helix 12 from 414 (in) to 429 (out) : -40.546 Transmembrane helix 13 from 433 (out) to 448 (in) : -45.362 Transmembrane helix 14 from 452 (in) to 467 (out) : -74.871 Transmembrane helix 15 from 471 (out) to 486 (in) : -187.395 Transmembrane helix 16 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 17 from 511 (out) to 526 (in) : -154.721 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 10 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 11 from 399 (in) to 414 (out) : -94.696 Transmembrane helix 12 from 418 (out) to 433 (in) : -42.207 Transmembrane helix 13 from 437 (in) to 452 (out) : -50.062 Transmembrane helix 14 from 456 (out) to 471 (in) : -79.017 Transmembrane helix 15 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 16 from 511 (out) to 526 (in) : -154.721 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 9 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 10 from 399 (in) to 414 (out) : -94.696 Transmembrane helix 11 from 418 (out) to 433 (in) : -42.207 Transmembrane helix 12 from 437 (in) to 452 (out) : -50.062 Transmembrane helix 13 from 456 (out) to 471 (in) : -79.017 Transmembrane helix 14 from 492 (in) to 507 (out) : -172.964 Transmembrane helix 15 from 511 (out) to 526 (in) : -154.721 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 10 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 11 from 405 (in) to 420 (out) : -68.088 Transmembrane helix 12 from 424 (out) to 439 (in) : -37.457 Transmembrane helix 13 from 443 (in) to 458 (out) : -52.185 Transmembrane helix 14 from 462 (out) to 477 (in) : -105.039 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 9 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 10 from 405 (in) to 420 (out) : -68.088 Transmembrane helix 11 from 424 (out) to 439 (in) : -37.457 Transmembrane helix 12 from 443 (in) to 458 (out) : -52.185 Transmembrane helix 13 from 462 (out) to 477 (in) : -105.039 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 7 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 8 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 9 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 10 from 423 (out) to 438 (in) : -38.627 Transmembrane helix 11 from 442 (in) to 457 (out) : -50.957 Transmembrane helix 12 from 462 (out) to 477 (in) : -105.039 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 6 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 7 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 8 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 9 from 423 (out) to 438 (in) : -38.627 Transmembrane helix 10 from 442 (in) to 457 (out) : -50.957 Transmembrane helix 11 from 462 (out) to 477 (in) : -105.039 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 87 (in) to 104 (out) : 498.986 Transmembrane helix 2 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 3 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 4 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 5 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 6 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 7 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 8 from 423 (out) to 438 (in) : -38.627 Transmembrane helix 9 from 442 (in) to 457 (out) : -50.957 Transmembrane helix 10 from 462 (out) to 477 (in) : -105.039 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 5 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 6 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 7 from 410 (out) to 425 (in) : -47.69 Transmembrane helix 8 from 429 (in) to 444 (out) : -41.616 Transmembrane helix 9 from 449 (out) to 464 (in) : -53.729 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 4 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 5 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 6 from 410 (out) to 425 (in) : -47.69 Transmembrane helix 7 from 429 (in) to 444 (out) : -41.616 Transmembrane helix 8 from 449 (out) to 464 (in) : -53.729 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 4 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 5 from 410 (out) to 425 (in) : -47.69 Transmembrane helix 6 from 429 (in) to 444 (out) : -41.616 Transmembrane helix 7 from 449 (out) to 464 (in) : -53.729 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 4 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 5 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 6 from 424 (out) to 439 (in) : -37.457 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 4 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 5 from 424 (out) to 439 (in) : -37.457 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 87 (in) to 104 (out) : 498.986 Transmembrane helix 2 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 3 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 4 from 326 (out) to 341 (in) : 0.863002 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 87 (in) to 104 (out) : 498.986 Transmembrane helix 2 from 228 (out) to 252 (in) : 861.715 Score = 1.360701 Processing 28 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -1337.41 Transmembrane helix 2 from 23 (in) to 38 (out) : -770.307 Transmembrane helix 3 from 42 (out) to 57 (in) : -357.256 Transmembrane helix 4 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 5 from 80 (out) to 95 (in) : 278.805 Transmembrane helix 6 from 99 (in) to 114 (out) : 24.15 Transmembrane helix 7 from 118 (out) to 133 (in) : -222.231 Transmembrane helix 8 from 137 (in) to 152 (out) : -634.897 Transmembrane helix 9 from 156 (out) to 171 (in) : -1436.26 Transmembrane helix 10 from 175 (in) to 190 (out) : -505.123 Transmembrane helix 11 from 194 (out) to 209 (in) : -169.441 Transmembrane helix 12 from 213 (in) to 228 (out) : -343.954 Transmembrane helix 13 from 232 (out) to 247 (in) : 629.117 Transmembrane helix 14 from 251 (in) to 266 (out) : -175.075 Transmembrane helix 15 from 270 (out) to 285 (in) : 104.228 Transmembrane helix 16 from 289 (in) to 304 (out) : -560.632 Transmembrane helix 17 from 308 (out) to 323 (in) : -256.479 Transmembrane helix 18 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 19 from 346 (out) to 361 (in) : -393.761 Transmembrane helix 20 from 365 (in) to 380 (out) : -296.543 Transmembrane helix 21 from 384 (out) to 399 (in) : -128.59 Transmembrane helix 22 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 23 from 422 (out) to 437 (in) : -39.672 Transmembrane helix 24 from 441 (in) to 456 (out) : -50.07 Transmembrane helix 25 from 460 (out) to 475 (in) : -98.371 Transmembrane helix 26 from 479 (in) to 494 (out) : -234.104 Transmembrane helix 27 from 498 (out) to 513 (in) : -144.951 Transmembrane helix 28 from 517 (in) to 532 (out) : -247.17 Score = -100000.000000 Processing 27 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -1248.99 Transmembrane helix 2 from 28 (out) to 43 (in) : -592.336 Transmembrane helix 3 from 47 (in) to 62 (out) : -265.359 Transmembrane helix 4 from 66 (out) to 81 (in) : -144.144 Transmembrane helix 5 from 85 (in) to 100 (out) : 461.118 Transmembrane helix 6 from 104 (out) to 119 (in) : -129.001 Transmembrane helix 7 from 123 (in) to 138 (out) : -375.261 Transmembrane helix 8 from 142 (out) to 157 (in) : -766.334 Transmembrane helix 9 from 175 (in) to 190 (out) : -505.123 Transmembrane helix 10 from 194 (out) to 209 (in) : -169.441 Transmembrane helix 11 from 213 (in) to 228 (out) : -343.954 Transmembrane helix 12 from 232 (out) to 247 (in) : 629.117 Transmembrane helix 13 from 251 (in) to 266 (out) : -175.075 Transmembrane helix 14 from 270 (out) to 285 (in) : 104.228 Transmembrane helix 15 from 289 (in) to 304 (out) : -560.632 Transmembrane helix 16 from 308 (out) to 323 (in) : -256.479 Transmembrane helix 17 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 18 from 346 (out) to 361 (in) : -393.761 Transmembrane helix 19 from 365 (in) to 380 (out) : -296.543 Transmembrane helix 20 from 384 (out) to 399 (in) : -128.59 Transmembrane helix 21 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 22 from 422 (out) to 437 (in) : -39.672 Transmembrane helix 23 from 441 (in) to 456 (out) : -50.07 Transmembrane helix 24 from 460 (out) to 475 (in) : -98.371 Transmembrane helix 25 from 479 (in) to 494 (out) : -234.104 Transmembrane helix 26 from 498 (out) to 513 (in) : -144.951 Transmembrane helix 27 from 517 (in) to 532 (out) : -247.17 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -592.336 Transmembrane helix 2 from 47 (in) to 62 (out) : -265.359 Transmembrane helix 3 from 66 (out) to 81 (in) : -144.144 Transmembrane helix 4 from 85 (in) to 100 (out) : 461.118 Transmembrane helix 5 from 104 (out) to 119 (in) : -129.001 Transmembrane helix 6 from 123 (in) to 138 (out) : -375.261 Transmembrane helix 7 from 142 (out) to 157 (in) : -766.334 Transmembrane helix 8 from 175 (in) to 190 (out) : -505.123 Transmembrane helix 9 from 194 (out) to 209 (in) : -169.441 Transmembrane helix 10 from 213 (in) to 228 (out) : -343.954 Transmembrane helix 11 from 232 (out) to 247 (in) : 629.117 Transmembrane helix 12 from 251 (in) to 266 (out) : -175.075 Transmembrane helix 13 from 270 (out) to 285 (in) : 104.228 Transmembrane helix 14 from 289 (in) to 304 (out) : -560.632 Transmembrane helix 15 from 308 (out) to 323 (in) : -256.479 Transmembrane helix 16 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 17 from 346 (out) to 361 (in) : -393.761 Transmembrane helix 18 from 365 (in) to 380 (out) : -296.543 Transmembrane helix 19 from 384 (out) to 399 (in) : -128.59 Transmembrane helix 20 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 21 from 422 (out) to 437 (in) : -39.672 Transmembrane helix 22 from 441 (in) to 456 (out) : -50.07 Transmembrane helix 23 from 460 (out) to 475 (in) : -98.371 Transmembrane helix 24 from 479 (in) to 494 (out) : -234.104 Transmembrane helix 25 from 498 (out) to 513 (in) : -144.951 Transmembrane helix 26 from 517 (in) to 532 (out) : -247.17 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -465.017 Transmembrane helix 2 from 54 (out) to 69 (in) : -204.864 Transmembrane helix 3 from 73 (in) to 88 (out) : 13.549 Transmembrane helix 4 from 92 (out) to 107 (in) : 380.592 Transmembrane helix 5 from 111 (in) to 126 (out) : -88.171 Transmembrane helix 6 from 130 (out) to 145 (in) : -616.94 Transmembrane helix 7 from 175 (in) to 190 (out) : -505.123 Transmembrane helix 8 from 194 (out) to 209 (in) : -169.441 Transmembrane helix 9 from 213 (in) to 228 (out) : -343.954 Transmembrane helix 10 from 232 (out) to 247 (in) : 629.117 Transmembrane helix 11 from 251 (in) to 266 (out) : -175.075 Transmembrane helix 12 from 270 (out) to 285 (in) : 104.228 Transmembrane helix 13 from 289 (in) to 304 (out) : -560.632 Transmembrane helix 14 from 308 (out) to 323 (in) : -256.479 Transmembrane helix 15 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 16 from 346 (out) to 361 (in) : -393.761 Transmembrane helix 17 from 365 (in) to 380 (out) : -296.543 Transmembrane helix 18 from 384 (out) to 399 (in) : -128.59 Transmembrane helix 19 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 20 from 422 (out) to 437 (in) : -39.672 Transmembrane helix 21 from 441 (in) to 456 (out) : -50.07 Transmembrane helix 22 from 460 (out) to 475 (in) : -98.371 Transmembrane helix 23 from 479 (in) to 494 (out) : -234.104 Transmembrane helix 24 from 498 (out) to 513 (in) : -144.951 Transmembrane helix 25 from 517 (in) to 532 (out) : -247.17 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 36 (out) to 51 (in) : -442.719 Transmembrane helix 2 from 55 (in) to 70 (out) : -200.306 Transmembrane helix 3 from 74 (out) to 89 (in) : 34.167 Transmembrane helix 4 from 93 (in) to 108 (out) : 328.738 Transmembrane helix 5 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 6 from 135 (in) to 150 (out) : -624.549 Transmembrane helix 7 from 180 (out) to 195 (in) : -237.266 Transmembrane helix 8 from 199 (in) to 214 (out) : -243.629 Transmembrane helix 9 from 218 (out) to 233 (in) : 42.468 Transmembrane helix 10 from 237 (in) to 252 (out) : 454.557 Transmembrane helix 11 from 256 (out) to 271 (in) : -265.775 Transmembrane helix 12 from 275 (in) to 290 (out) : 45.861 Transmembrane helix 13 from 302 (out) to 317 (in) : -204.524 Transmembrane helix 14 from 321 (in) to 336 (out) : -44.704 Transmembrane helix 15 from 340 (out) to 355 (in) : -356.168 Transmembrane helix 16 from 359 (in) to 374 (out) : -322.371 Transmembrane helix 17 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 18 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 19 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 20 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 21 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 22 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 23 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 24 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -465.017 Transmembrane helix 2 from 54 (out) to 69 (in) : -204.864 Transmembrane helix 3 from 73 (in) to 88 (out) : 13.549 Transmembrane helix 4 from 92 (out) to 107 (in) : 380.592 Transmembrane helix 5 from 111 (in) to 126 (out) : -88.171 Transmembrane helix 6 from 130 (out) to 145 (in) : -616.94 Transmembrane helix 7 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 8 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 9 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 10 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 11 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 12 from 302 (out) to 317 (in) : -204.524 Transmembrane helix 13 from 321 (in) to 336 (out) : -44.704 Transmembrane helix 14 from 340 (out) to 355 (in) : -356.168 Transmembrane helix 15 from 359 (in) to 374 (out) : -322.371 Transmembrane helix 16 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 17 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 18 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 19 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 20 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 21 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 22 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 23 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 36 (out) to 51 (in) : -442.719 Transmembrane helix 2 from 55 (in) to 70 (out) : -200.306 Transmembrane helix 3 from 74 (out) to 89 (in) : 34.167 Transmembrane helix 4 from 93 (in) to 108 (out) : 328.738 Transmembrane helix 5 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 6 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 7 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 8 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 9 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 10 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 11 from 302 (out) to 317 (in) : -204.524 Transmembrane helix 12 from 321 (in) to 336 (out) : -44.704 Transmembrane helix 13 from 340 (out) to 355 (in) : -356.168 Transmembrane helix 14 from 359 (in) to 374 (out) : -322.371 Transmembrane helix 15 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 16 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 17 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 18 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 19 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 20 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 21 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 22 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 184 (in) to 199 (out) : -125.259 Transmembrane helix 6 from 203 (out) to 218 (in) : -318.44 Transmembrane helix 7 from 227 (in) to 242 (out) : 645.229 Transmembrane helix 8 from 246 (out) to 261 (in) : 68.884 Transmembrane helix 9 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 10 from 302 (out) to 317 (in) : -204.524 Transmembrane helix 11 from 321 (in) to 336 (out) : -44.704 Transmembrane helix 12 from 340 (out) to 355 (in) : -356.168 Transmembrane helix 13 from 359 (in) to 374 (out) : -322.371 Transmembrane helix 14 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 15 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 16 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 17 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 18 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 19 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 20 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 21 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 36 (out) to 51 (in) : -442.719 Transmembrane helix 2 from 55 (in) to 70 (out) : -200.306 Transmembrane helix 3 from 74 (out) to 89 (in) : 34.167 Transmembrane helix 4 from 93 (in) to 108 (out) : 328.738 Transmembrane helix 5 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 6 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 7 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 8 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 9 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 10 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 11 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 12 from 358 (in) to 373 (out) : -320.367 Transmembrane helix 13 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 14 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 15 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 16 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 17 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 18 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 19 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 20 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 10 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 11 from 358 (in) to 373 (out) : -320.367 Transmembrane helix 12 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 13 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 14 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 15 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 16 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 17 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 18 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 19 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 9 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 10 from 358 (in) to 373 (out) : -320.367 Transmembrane helix 11 from 378 (out) to 393 (in) : -132.769 Transmembrane helix 12 from 397 (in) to 412 (out) : -104.192 Transmembrane helix 13 from 416 (out) to 431 (in) : -43.176 Transmembrane helix 14 from 435 (in) to 450 (out) : -47.932 Transmembrane helix 15 from 454 (out) to 469 (in) : -70.392 Transmembrane helix 16 from 473 (in) to 488 (out) : -204.731 Transmembrane helix 17 from 492 (out) to 507 (in) : -185.936 Transmembrane helix 18 from 511 (in) to 526 (out) : -165.679 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 10 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 11 from 379 (in) to 394 (out) : -125.645 Transmembrane helix 12 from 398 (out) to 413 (in) : -102.091 Transmembrane helix 13 from 417 (in) to 432 (out) : -38.836 Transmembrane helix 14 from 436 (out) to 451 (in) : -47.108 Transmembrane helix 15 from 455 (in) to 470 (out) : -81.261 Transmembrane helix 16 from 474 (out) to 489 (in) : -216.129 Transmembrane helix 17 from 504 (in) to 519 (out) : -125.839 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 303 (in) to 318 (out) : -186.608 Transmembrane helix 9 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 10 from 379 (in) to 394 (out) : -125.645 Transmembrane helix 11 from 398 (out) to 413 (in) : -102.091 Transmembrane helix 12 from 417 (in) to 432 (out) : -38.836 Transmembrane helix 13 from 436 (out) to 451 (in) : -47.108 Transmembrane helix 14 from 455 (in) to 470 (out) : -81.261 Transmembrane helix 15 from 474 (out) to 489 (in) : -216.129 Transmembrane helix 16 from 504 (in) to 519 (out) : -125.839 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 7 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 8 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 9 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 10 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 11 from 399 (in) to 414 (out) : -94.696 Transmembrane helix 12 from 418 (out) to 433 (in) : -42.207 Transmembrane helix 13 from 437 (in) to 452 (out) : -50.062 Transmembrane helix 14 from 456 (out) to 471 (in) : -79.017 Transmembrane helix 15 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 227 (out) to 242 (in) : 634.671 Transmembrane helix 6 from 246 (in) to 261 (out) : 43.316 Transmembrane helix 7 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 8 from 327 (in) to 342 (out) : 9.626 Transmembrane helix 9 from 380 (out) to 395 (in) : -123.33 Transmembrane helix 10 from 399 (in) to 414 (out) : -94.696 Transmembrane helix 11 from 418 (out) to 433 (in) : -42.207 Transmembrane helix 12 from 437 (in) to 452 (out) : -50.062 Transmembrane helix 13 from 456 (out) to 471 (in) : -79.017 Transmembrane helix 14 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 53 (in) to 68 (out) : -205.481 Transmembrane helix 2 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 3 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 4 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 6 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 7 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 8 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 9 from 395 (in) to 410 (out) : -112.53 Transmembrane helix 10 from 414 (out) to 429 (in) : -44.954 Transmembrane helix 11 from 433 (in) to 448 (out) : -44.638 Transmembrane helix 12 from 452 (out) to 467 (in) : -62.729 Transmembrane helix 13 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 6 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 7 from 326 (out) to 341 (in) : 0.863002 Transmembrane helix 8 from 395 (in) to 410 (out) : -112.53 Transmembrane helix 9 from 414 (out) to 429 (in) : -44.954 Transmembrane helix 10 from 433 (in) to 448 (out) : -44.638 Transmembrane helix 11 from 452 (out) to 467 (in) : -62.729 Transmembrane helix 12 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 111 (in) to 126 (out) : -88.171 Transmembrane helix 4 from 188 (out) to 203 (in) : -118.378 Transmembrane helix 5 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 6 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 7 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 8 from 409 (out) to 424 (in) : -48.631 Transmembrane helix 9 from 428 (in) to 443 (out) : -41.012 Transmembrane helix 10 from 447 (out) to 462 (in) : -55.242 Transmembrane helix 11 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 72 (out) to 87 (in) : -32.638 Transmembrane helix 2 from 91 (in) to 106 (out) : 419.276 Transmembrane helix 3 from 112 (out) to 127 (in) : -81.633 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.738 Transmembrane helix 5 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 6 from 272 (in) to 287 (out) : 145.492 Transmembrane helix 7 from 327 (out) to 342 (in) : -1.226 Transmembrane helix 8 from 403 (in) to 418 (out) : -73.764 Transmembrane helix 9 from 423 (out) to 438 (in) : -38.627 Transmembrane helix 10 from 442 (in) to 457 (out) : -50.957 Re-entrant helix 1 from 291 (out) to 318 (out) : 189.9 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 4 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 5 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 6 from 409 (out) to 424 (in) : -48.631 Transmembrane helix 7 from 428 (in) to 443 (out) : -41.012 Transmembrane helix 8 from 447 (out) to 462 (in) : -55.242 Transmembrane helix 9 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 4 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 5 from 409 (out) to 424 (in) : -48.631 Transmembrane helix 6 from 428 (in) to 443 (out) : -41.012 Transmembrane helix 7 from 447 (out) to 462 (in) : -55.242 Transmembrane helix 8 from 504 (in) to 519 (out) : -125.839 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 4 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 5 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 6 from 415 (out) to 430 (in) : -43.639 Transmembrane helix 7 from 434 (in) to 449 (out) : -45.769 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 4 from 326 (in) to 341 (out) : 11.037 Transmembrane helix 5 from 415 (out) to 430 (in) : -43.639 Transmembrane helix 6 from 434 (in) to 449 (out) : -45.769 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 61 (in) to 76 (out) : -177.719 Transmembrane helix 2 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 3 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 4 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 5 from 326 (in) to 341 (out) : 11.037 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Transmembrane helix 3 from 272 (out) to 287 (in) : 140.508 Transmembrane helix 4 from 326 (in) to 341 (out) : 11.037 Re-entrant helix 1 from 291 (in) to 318 (in) : 189.9 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 87 (in) to 104 (out) : 498.986 Transmembrane helix 2 from 228 (out) to 252 (in) : 861.715 Transmembrane helix 3 from 272 (in) to 287 (out) : 145.492 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 87 (out) to 104 (in) : 516.214 Transmembrane helix 2 from 228 (in) to 252 (out) : 868.685 Score = 1.384899 Summary of topology analysis: 1 helix (+) : Score = 0.868685 1 helix (-) : Score = 0.861715 2 helices (+) : Score = 1.3607 2 helices (-) : Score = 1.3849 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 24 helices (+) : Score = -100000 24 helices (-) : Score = -100000 25 helices (+) : Score = -100000 25 helices (-) : Score = -100000 26 helices (+) : Score = -100000 26 helices (-) : Score = -100000 27 helices (+) : Score = -100000 27 helices (-) : Score = -100000 28 helices (+) : Score = -100000 28 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 87-104,228-252 Re-entrant helices: Not detected. Helix count: 2 N-terminal: out Score: 1.3849