BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (82 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P75994 Uncharacterized protein ymgC n=35 Tax=Enterobact...   167   8e-41
UniRef50_C4X5R2 Putative uncharacterized protein n=4 Tax=Klebsie...    47   1e-04

>UniRef50_P75994 Uncharacterized protein ymgC n=35 Tax=Enterobacteriaceae
          RepID=YMGC_ECOLI
          Length = 82

 Score =  167 bits (424), Expect = 8e-41,   Method: Compositional matrix adjust.
 Identities = 82/82 (100%), Positives = 82/82 (100%)

Query: 1  MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ 60
          MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ
Sbjct: 1  MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ 60

Query: 61 YIEMHKTTLTDIIINDRESVLS 82
          YIEMHKTTLTDIIINDRESVLS
Sbjct: 61 YIEMHKTTLTDIIINDRESVLS 82


>UniRef50_C4X5R2 Putative uncharacterized protein n=4 Tax=Klebsiella
           RepID=C4X5R2_KLEPN
          Length = 121

 Score = 47.4 bits (111), Expect = 1e-04,   Method: Compositional matrix adjust.
 Identities = 24/59 (40%), Positives = 37/59 (62%)

Query: 1   MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQIL 59
           + N+IP  F+F  AL KN+E  +   H  V++  +   ++ RL+ E++ ILSDLYLQ L
Sbjct: 54  IKNTIPAEFVFNSALMKNIENTLIKQHRTVNNERMITEIQHRLQTESNEILSDLYLQAL 112


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P75994 Uncharacterized protein ymgC n=35 Tax=Enterobact...   122   3e-27
UniRef50_C4X5R2 Putative uncharacterized protein n=4 Tax=Klebsie...    92   6e-18

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P75994 Uncharacterized protein ymgC n=35 Tax=Enterobacteriaceae
          RepID=YMGC_ECOLI
          Length = 82

 Score =  122 bits (307), Expect = 3e-27,   Method: Composition-based stats.
 Identities = 82/82 (100%), Positives = 82/82 (100%)

Query: 1  MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ 60
          MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ
Sbjct: 1  MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ 60

Query: 61 YIEMHKTTLTDIIINDRESVLS 82
          YIEMHKTTLTDIIINDRESVLS
Sbjct: 61 YIEMHKTTLTDIIINDRESVLS 82


>UniRef50_C4X5R2 Putative uncharacterized protein n=4 Tax=Klebsiella
           RepID=C4X5R2_KLEPN
          Length = 121

 Score = 91.6 bits (226), Expect = 6e-18,   Method: Composition-based stats.
 Identities = 24/62 (38%), Positives = 38/62 (61%)

Query: 1   MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQ 60
           + N+IP  F+F  AL KN+E  +   H  V++  +   ++ RL+ E++ ILSDLYLQ L 
Sbjct: 54  IKNTIPAEFVFNSALMKNIENTLIKQHRTVNNERMITEIQHRLQTESNEILSDLYLQALD 113

Query: 61  YI 62
            +
Sbjct: 114 ML 115


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.319    0.137    0.352 

Lambda     K      H
   0.267   0.0425    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 286,327,688
Number of Sequences: 3077464
Number of extensions: 8395192
Number of successful extensions: 29780
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 29776
Number of HSP's gapped (non-prelim): 4
length of query: 82
length of database: 1,040,396,356
effective HSP length: 53
effective length of query: 29
effective length of database: 877,290,764
effective search space: 25441432156
effective search space used: 25441432156
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.3 bits)
S2: 87 (38.1 bits)