Processing 1 helix: Transmembrane helix 1 from 241 (in) to 256 (out) : -37.528 Score = -0.041407 Processing 1 helix: Transmembrane helix 1 from 241 (out) to 256 (in) : -34.572 Score = -0.030693 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 80 (in) to 95 (out) : -1280.77 Transmembrane helix 6 from 99 (out) to 114 (in) : -343.106 Transmembrane helix 7 from 118 (in) to 133 (out) : -779.262 Transmembrane helix 8 from 137 (out) to 152 (in) : -1117.63 Transmembrane helix 9 from 158 (in) to 173 (out) : -1382.67 Transmembrane helix 10 from 177 (out) to 192 (in) : -920.988 Transmembrane helix 11 from 204 (in) to 219 (out) : -1256.39 Transmembrane helix 12 from 223 (out) to 238 (in) : -357.429 Transmembrane helix 13 from 242 (in) to 257 (out) : -37.87 Transmembrane helix 14 from 261 (out) to 276 (in) : -770.904 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 80 (out) to 95 (in) : -1288.23 Transmembrane helix 6 from 99 (in) to 114 (out) : -332.494 Transmembrane helix 7 from 118 (out) to 133 (in) : -768.538 Transmembrane helix 8 from 137 (in) to 152 (out) : -1116.67 Transmembrane helix 9 from 171 (out) to 186 (in) : -799.797 Transmembrane helix 10 from 204 (in) to 219 (out) : -1256.39 Transmembrane helix 11 from 223 (out) to 238 (in) : -357.429 Transmembrane helix 12 from 242 (in) to 257 (out) : -37.87 Transmembrane helix 13 from 261 (out) to 276 (in) : -770.904 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 95 (in) to 110 (out) : -413.34 Transmembrane helix 6 from 114 (out) to 129 (in) : -579.637 Transmembrane helix 7 from 134 (in) to 149 (out) : -1095.32 Transmembrane helix 8 from 171 (out) to 186 (in) : -799.797 Transmembrane helix 9 from 204 (in) to 219 (out) : -1256.39 Transmembrane helix 10 from 223 (out) to 238 (in) : -357.429 Transmembrane helix 11 from 242 (in) to 257 (out) : -37.87 Transmembrane helix 12 from 261 (out) to 276 (in) : -770.904 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 95 (out) to 110 (in) : -421.96 Transmembrane helix 6 from 114 (in) to 129 (out) : -592.163 Transmembrane helix 7 from 134 (out) to 149 (in) : -1100.08 Transmembrane helix 8 from 171 (in) to 186 (out) : -796.903 Transmembrane helix 9 from 220 (out) to 235 (in) : -411.066 Transmembrane helix 10 from 239 (in) to 254 (out) : -50.958 Transmembrane helix 11 from 258 (out) to 273 (in) : -630.746 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 95 (in) to 110 (out) : -413.34 Transmembrane helix 6 from 114 (out) to 129 (in) : -579.637 Transmembrane helix 7 from 171 (in) to 186 (out) : -796.903 Transmembrane helix 8 from 220 (out) to 235 (in) : -411.066 Transmembrane helix 9 from 239 (in) to 254 (out) : -50.958 Transmembrane helix 10 from 258 (out) to 273 (in) : -630.746 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 95 (out) to 110 (in) : -421.96 Transmembrane helix 6 from 114 (in) to 129 (out) : -592.163 Transmembrane helix 7 from 220 (out) to 235 (in) : -411.066 Transmembrane helix 8 from 239 (in) to 254 (out) : -50.958 Transmembrane helix 9 from 258 (out) to 273 (in) : -630.746 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 95 (in) to 110 (out) : -413.34 Transmembrane helix 6 from 114 (out) to 129 (in) : -579.637 Transmembrane helix 7 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 8 from 242 (out) to 257 (in) : -37.53 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 100 (out) to 115 (in) : -337.131 Transmembrane helix 6 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 7 from 242 (out) to 257 (in) : -37.53 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 27 (out) to 42 (in) : -203.145 Transmembrane helix 3 from 46 (in) to 61 (out) : -189.873 Transmembrane helix 4 from 100 (out) to 115 (in) : -337.131 Transmembrane helix 5 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 6 from 242 (out) to 257 (in) : -37.53 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 31 (in) to 46 (out) : -172.977 Transmembrane helix 3 from 50 (out) to 65 (in) : -229.98 Transmembrane helix 4 from 100 (in) to 115 (out) : -327.169 Transmembrane helix 5 from 241 (out) to 256 (in) : -34.572 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 27 (out) to 42 (in) : -203.145 Transmembrane helix 3 from 46 (in) to 61 (out) : -189.873 Transmembrane helix 4 from 241 (out) to 256 (in) : -34.572 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 37 (in) to 52 (out) : -134.47 Transmembrane helix 3 from 241 (out) to 256 (in) : -34.572 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 37 (in) to 52 (out) : -134.47 Transmembrane helix 2 from 241 (out) to 256 (in) : -34.572 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 80 (out) to 95 (in) : -1288.23 Transmembrane helix 6 from 99 (in) to 114 (out) : -332.494 Transmembrane helix 7 from 118 (out) to 133 (in) : -768.538 Transmembrane helix 8 from 137 (in) to 152 (out) : -1116.67 Transmembrane helix 9 from 157 (out) to 172 (in) : -1411.32 Transmembrane helix 10 from 176 (in) to 191 (out) : -888.479 Transmembrane helix 11 from 204 (out) to 219 (in) : -1252.71 Transmembrane helix 12 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 13 from 242 (out) to 257 (in) : -37.53 Transmembrane helix 14 from 261 (in) to 276 (out) : -774.696 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 91 (in) to 106 (out) : -671.092 Transmembrane helix 6 from 110 (out) to 125 (in) : -446.369 Transmembrane helix 7 from 129 (in) to 144 (out) : -1038.38 Transmembrane helix 8 from 148 (out) to 163 (in) : -1366.84 Transmembrane helix 9 from 171 (in) to 186 (out) : -796.903 Transmembrane helix 10 from 204 (out) to 219 (in) : -1252.71 Transmembrane helix 11 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 12 from 242 (out) to 257 (in) : -37.53 Transmembrane helix 13 from 261 (in) to 276 (out) : -774.696 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 95 (out) to 110 (in) : -421.96 Transmembrane helix 6 from 114 (in) to 129 (out) : -592.163 Transmembrane helix 7 from 134 (out) to 149 (in) : -1100.08 Transmembrane helix 8 from 171 (in) to 186 (out) : -796.903 Transmembrane helix 9 from 204 (out) to 219 (in) : -1252.71 Transmembrane helix 10 from 223 (in) to 238 (out) : -349.971 Transmembrane helix 11 from 242 (out) to 257 (in) : -37.53 Transmembrane helix 12 from 261 (in) to 276 (out) : -774.696 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 95 (in) to 110 (out) : -413.34 Transmembrane helix 6 from 114 (out) to 129 (in) : -579.637 Transmembrane helix 7 from 134 (in) to 149 (out) : -1095.32 Transmembrane helix 8 from 171 (out) to 186 (in) : -799.797 Transmembrane helix 9 from 220 (in) to 235 (out) : -402.534 Transmembrane helix 10 from 239 (out) to 254 (in) : -45.342 Transmembrane helix 11 from 258 (in) to 273 (out) : -625.454 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 95 (out) to 110 (in) : -421.96 Transmembrane helix 6 from 114 (in) to 129 (out) : -592.163 Transmembrane helix 7 from 171 (out) to 186 (in) : -799.797 Transmembrane helix 8 from 220 (in) to 235 (out) : -402.534 Transmembrane helix 9 from 239 (out) to 254 (in) : -45.342 Transmembrane helix 10 from 258 (in) to 273 (out) : -625.454 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 95 (in) to 110 (out) : -413.34 Transmembrane helix 6 from 114 (out) to 129 (in) : -579.637 Transmembrane helix 7 from 220 (in) to 235 (out) : -402.534 Transmembrane helix 8 from 239 (out) to 254 (in) : -45.342 Transmembrane helix 9 from 258 (in) to 273 (out) : -625.454 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 23 (in) to 38 (out) : -252.46 Transmembrane helix 3 from 42 (out) to 57 (in) : -180.413 Transmembrane helix 4 from 61 (in) to 76 (out) : -445.572 Transmembrane helix 5 from 100 (out) to 115 (in) : -337.131 Transmembrane helix 6 from 220 (in) to 235 (out) : -402.534 Transmembrane helix 7 from 239 (out) to 254 (in) : -45.342 Transmembrane helix 8 from 258 (in) to 273 (out) : -625.454 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 23 (out) to 38 (in) : -244.64 Transmembrane helix 3 from 42 (in) to 57 (out) : -169.887 Transmembrane helix 4 from 61 (out) to 76 (in) : -444.028 Transmembrane helix 5 from 100 (in) to 115 (out) : -327.169 Transmembrane helix 6 from 227 (out) to 242 (in) : -289.034 Transmembrane helix 7 from 246 (in) to 261 (out) : -108.067 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 31 (in) to 46 (out) : -172.977 Transmembrane helix 3 from 50 (out) to 65 (in) : -229.98 Transmembrane helix 4 from 100 (in) to 115 (out) : -327.169 Transmembrane helix 5 from 227 (out) to 242 (in) : -289.034 Transmembrane helix 6 from 246 (in) to 261 (out) : -108.067 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 27 (out) to 42 (in) : -203.145 Transmembrane helix 3 from 46 (in) to 61 (out) : -189.873 Transmembrane helix 4 from 227 (out) to 242 (in) : -289.034 Transmembrane helix 5 from 246 (in) to 261 (out) : -108.067 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 31 (in) to 46 (out) : -172.977 Transmembrane helix 3 from 50 (out) to 65 (in) : -229.98 Transmembrane helix 4 from 241 (in) to 256 (out) : -37.528 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -168.613 Transmembrane helix 2 from 36 (out) to 51 (in) : -145.426 Transmembrane helix 3 from 241 (in) to 256 (out) : -37.528 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -153.387 Transmembrane helix 2 from 241 (in) to 256 (out) : -37.528 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.041407 1 helix (-) : Score = -0.030693 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 241-256 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.030693