Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	N	R	N	L	L	K	F	L	P	G	L	L	I	C	L	I	V	L	T	S	C	V	P	K	Q	K	N	M	P	Y	A	L	T	Q	R	S	I	P	Q	I	L	P	L	P	S	E	A	K	Q	P	K	P	P	K	E	C	S	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?																			?									?												?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.
Sequence		T	C	S	E	I	L	Q	Q	K	L	S	F	M	L	K	L	L	T	N	A	T	S	Q	E
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1sxm chain A Region: 55 - 65 Aligned: 11 Modelled: 11 Confidence: 22.9% Identity: 55% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Scorpion toxin-like Family: Short-chain scorpion toxins Phyre2 PDB 1zll chain E Region: 13 - 20 Aligned: 8 Modelled: 8 Confidence: 11.6% Identity: 75% PDB header:membrane protein/signaling protein Chain: E: PDB Molecule:cardiac phospholamban; PDBTitle: nmr structure of unphosphorylated human phospholamban2 pentamer Phyre2 PDB 1jli chain A Region: 66 - 84 Aligned: 19 Modelled: 19 Confidence: 9.5% Identity: 47% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Short-chain cytokines Phyre2 PDB 2cs3 chain A domain 1 Region: 23 - 43 Aligned: 21 Modelled: 21 Confidence: 9.5% Identity: 38% Fold: RING/U-box Superfamily: RING/U-box Family: Variant RING domain Phyre2 PDB 3h0d chain B Region: 22 - 37 Aligned: 14 Modelled: 16 Confidence: 8.4% Identity: 50% PDB header:transcription/dna Chain: B: PDB Molecule:ctsr; PDBTitle: crystal structure of ctsr in complex with a 26bp dna duplex Phyre2 PDB 2b0j chain A domain 2 Region: 52 - 58 Aligned: 7 Modelled: 7 Confidence: 7.0% Identity: 86% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: 6-phosphogluconate dehydrogenase-like, N-terminal domain Phyre2 PDB 3f46 chain A Region: 52 - 58 Aligned: 7 Modelled: 7 Confidence: 6.0% Identity: 86% PDB header:oxidoreductase Chain: A: PDB Molecule:5,10-methenyltetrahydromethanopterin hydrogenase; PDBTitle: the crystal structure of c176a mutated [fe]-hydrogenase (hmd)2 holoenzyme from methanocaldococcus jannaschii Phyre2 PDB 2cv4 chain A domain 1 Region: 55 - 65 Aligned: 11 Modelled: 11 Confidence: 5.9% Identity: 27% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: Glutathione peroxidase-like Phyre2 1 d1sxma_ 2 c1zllE_ 3 d1jlia_ 4 d2cs3a1 5 c3h0dB_ 6 d2b0ja2 7 c3f46A_ 8 d2cv4a1

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1sxma_ 22.9 55 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 2 c1zllE_ 11.6 75 PDB header:membrane protein/signaling protein Chain: E: PDB Molecule:cardiac phospholamban; PDBTitle: nmr structure of unphosphorylated human phospholamban2 pentamer 3 d1jlia_ 9.5 47 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Short-chain cytokines 4 d2cs3a1 9.5 38 Fold:RING/U-box Superfamily:RING/U-box Family:Variant RING domain 5 c3h0dB_ 8.4 50 PDB header:transcription/dna Chain: B: PDB Molecule:ctsr; PDBTitle: crystal structure of ctsr in complex with a 26bp dna duplex 6 d2b0ja2 7.0 86 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:6-phosphogluconate dehydrogenase-like, N-terminal domain 7 c3f46A_ 6.0 86 PDB header:oxidoreductase Chain: A: PDB Molecule:5,10-methenyltetrahydromethanopterin hydrogenase; PDBTitle: the crystal structure of c176a mutated [fe]-hydrogenase (hmd)2 holoenzyme from methanocaldococcus jannaschii 8 d2cv4a1 5.9 27 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Glutathione peroxidase-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0