Processing 1 helix: Transmembrane helix 1 from 145 (in) to 175 (out) : 5161.22 Score = 5.161223 Processing 1 helix: Transmembrane helix 1 from 145 (out) to 175 (in) : 5197.58 Score = 5.197576 Processing 22 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -429.359 Transmembrane helix 2 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 3 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 4 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 5 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 6 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 7 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 8 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 9 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 10 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 11 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 12 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 13 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 14 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 15 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 16 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 17 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 18 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 19 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 20 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 21 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 22 from 1 (out) to 0 (in) : 3.791 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 68 (in) to 83 (out) : 2197.37 Transmembrane helix 5 from 87 (out) to 102 (in) : 1774.76 Transmembrane helix 6 from 106 (in) to 121 (out) : 1331.21 Transmembrane helix 7 from 125 (out) to 140 (in) : 240.488 Transmembrane helix 8 from 144 (in) to 159 (out) : 1544.36 Transmembrane helix 9 from 163 (out) to 178 (in) : 4291.32 Transmembrane helix 10 from 182 (in) to 197 (out) : -1002.55 Transmembrane helix 11 from 201 (out) to 216 (in) : 625.874 Transmembrane helix 12 from 220 (in) to 235 (out) : 2504.27 Transmembrane helix 13 from 239 (out) to 254 (in) : 205.878 Transmembrane helix 14 from 258 (in) to 273 (out) : 2556.82 Transmembrane helix 15 from 278 (out) to 293 (in) : 2530.34 Transmembrane helix 16 from 297 (in) to 312 (out) : 1463.63 Transmembrane helix 17 from 316 (out) to 331 (in) : 2301.26 Transmembrane helix 18 from 335 (in) to 350 (out) : 73.317 Transmembrane helix 19 from 354 (out) to 369 (in) : 2020.93 Transmembrane helix 20 from 373 (in) to 388 (out) : 1432.91 Transmembrane helix 21 from 392 (out) to 407 (in) : 2442.21 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 130 (in) to 145 (out) : 1664.73 Transmembrane helix 8 from 149 (out) to 164 (in) : 1236.63 Transmembrane helix 9 from 168 (in) to 183 (out) : 3282.51 Transmembrane helix 10 from 190 (out) to 205 (in) : -871.312 Transmembrane helix 11 from 209 (in) to 224 (out) : 2603.49 Transmembrane helix 12 from 228 (out) to 243 (in) : 711.539 Transmembrane helix 13 from 247 (in) to 262 (out) : 1977.43 Transmembrane helix 14 from 266 (out) to 281 (in) : 718.86 Transmembrane helix 15 from 285 (in) to 301 (out) : 3360.1 Transmembrane helix 16 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 17 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 18 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 19 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 20 from 381 (out) to 401 (in) : 5129.23 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 87 (out) : 2770.33 Transmembrane helix 5 from 91 (out) to 106 (in) : 2213.63 Transmembrane helix 6 from 110 (in) to 125 (out) : 482.734 Transmembrane helix 7 from 130 (out) to 145 (in) : 1658.07 Transmembrane helix 8 from 149 (in) to 164 (out) : 1233.97 Transmembrane helix 9 from 168 (out) to 183 (in) : 3315.19 Transmembrane helix 10 from 198 (in) to 213 (out) : 63.839 Transmembrane helix 11 from 217 (out) to 232 (in) : 2901.29 Transmembrane helix 12 from 236 (in) to 251 (out) : -109.33 Transmembrane helix 13 from 255 (out) to 271 (in) : 3091.1 Transmembrane helix 14 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 15 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 16 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 17 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 18 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 19 from 381 (out) to 401 (in) : 5129.23 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 8 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 9 from 198 (in) to 213 (out) : 63.839 Transmembrane helix 10 from 217 (out) to 232 (in) : 2901.29 Transmembrane helix 11 from 236 (in) to 251 (out) : -109.33 Transmembrane helix 12 from 255 (out) to 271 (in) : 3091.1 Transmembrane helix 13 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 14 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 15 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 16 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 17 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 18 from 381 (out) to 401 (in) : 5129.23 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 5 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 6 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 7 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 8 from 198 (in) to 213 (out) : 63.839 Transmembrane helix 9 from 217 (out) to 232 (in) : 2901.29 Transmembrane helix 10 from 236 (in) to 251 (out) : -109.33 Transmembrane helix 11 from 255 (out) to 271 (in) : 3091.1 Transmembrane helix 12 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 13 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 14 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 15 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 16 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 17 from 381 (out) to 401 (in) : 5129.23 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 8 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 9 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 10 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 11 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 12 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 13 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 14 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 15 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 16 from 381 (out) to 401 (in) : 5129.23 Score = 38.874718 Processing 15 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 5 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 6 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 7 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 8 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 9 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 10 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 11 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 12 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 13 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 14 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 15 from 381 (out) to 401 (in) : 5129.23 Score = 39.612629 Processing 14 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 4 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 5 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 6 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 7 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 8 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 9 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 10 from 305 (out) to 320 (in) : 2283.28 Transmembrane helix 11 from 324 (in) to 339 (out) : 618.513 Transmembrane helix 12 from 343 (out) to 358 (in) : 1356.77 Transmembrane helix 13 from 362 (in) to 377 (out) : 859.013 Transmembrane helix 14 from 381 (out) to 401 (in) : 5129.23 Score = 40.245605 Processing 13 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 5 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 6 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 7 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 8 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 9 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 10 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 11 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 12 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 13 from 379 (out) to 401 (in) : 5150.45 Score = 40.826012 Processing 12 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 4 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 5 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 6 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 7 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 8 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 9 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 10 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 11 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 12 from 379 (out) to 401 (in) : 5150.45 Score = 41.458988 Processing 11 helices: Transmembrane helix 1 from 5 (out) to 29 (in) : 2589.79 Transmembrane helix 2 from 39 (in) to 61 (out) : 2451.87 Transmembrane helix 3 from 76 (out) to 106 (in) : 4406.67 Transmembrane helix 4 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 5 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 6 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 7 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 8 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 9 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 10 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 11 from 379 (out) to 401 (in) : 5150.45 Score = 39.595970 Processing 10 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 4 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 5 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 6 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 7 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 8 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 9 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 10 from 379 (out) to 401 (in) : 5150.45 Score = 37.390713 Processing 9 helices: Transmembrane helix 1 from 16 (out) to 46 (in) : 2601.02 Transmembrane helix 2 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 3 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 4 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 5 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 6 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 7 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 8 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 9 from 379 (out) to 401 (in) : 5150.45 Score = 34.896702 Processing 8 helices: Transmembrane helix 1 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 2 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 3 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 4 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 5 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 6 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 7 from 346 (in) to 369 (out) : 2717.11 Transmembrane helix 8 from 379 (out) to 401 (in) : 5150.45 Score = 32.265221 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 46 (in) : 2601.02 Transmembrane helix 2 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 3 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 4 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 5 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 6 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 7 from 379 (out) to 401 (in) : 5150.45 Score = 29.689339 Processing 6 helices: Transmembrane helix 1 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 2 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 3 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 4 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 5 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 6 from 379 (out) to 401 (in) : 5150.45 Score = 27.057856 Processing 5 helices: Transmembrane helix 1 from 76 (out) to 106 (in) : 4406.67 Transmembrane helix 2 from 145 (in) to 175 (out) : 5161.22 Transmembrane helix 3 from 210 (out) to 235 (in) : 3804.97 Transmembrane helix 4 from 286 (in) to 316 (out) : 4690.67 Transmembrane helix 5 from 377 (out) to 401 (in) : 5125.9 Score = 23.194284 Processing 4 helices: Transmembrane helix 1 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 2 from 146 (out) to 176 (in) : 5196.92 Transmembrane helix 3 from 286 (in) to 316 (out) : 4690.67 Transmembrane helix 4 from 377 (out) to 401 (in) : 5125.9 Score = 19.555988 Processing 3 helices: Transmembrane helix 1 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 2 from 286 (in) to 316 (out) : 4690.67 Transmembrane helix 3 from 377 (out) to 401 (in) : 5125.9 Score = 15.137805 Processing 2 helices: Transmembrane helix 1 from 145 (in) to 175 (out) : 5161.22 Transmembrane helix 2 from 378 (out) to 401 (in) : 5151.09 Score = 10.356145 Processing 22 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -419.948 Transmembrane helix 2 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 3 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 4 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 5 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 6 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 7 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 8 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 9 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 10 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 11 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 12 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 13 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 14 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 15 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 16 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 17 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 18 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 19 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 20 from 1 (in) to 0 (out) : -6.954 Transmembrane helix 21 from 1 (out) to 0 (in) : 3.791 Transmembrane helix 22 from 1 (in) to 0 (out) : -6.954 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 130 (in) to 145 (out) : 1664.73 Transmembrane helix 8 from 149 (out) to 164 (in) : 1236.63 Transmembrane helix 9 from 168 (in) to 183 (out) : 3282.51 Transmembrane helix 10 from 190 (out) to 205 (in) : -871.312 Transmembrane helix 11 from 209 (in) to 224 (out) : 2603.49 Transmembrane helix 12 from 228 (out) to 243 (in) : 711.539 Transmembrane helix 13 from 247 (in) to 262 (out) : 1977.43 Transmembrane helix 14 from 266 (out) to 281 (in) : 718.86 Transmembrane helix 15 from 285 (in) to 300 (out) : 3331.93 Transmembrane helix 16 from 304 (out) to 319 (in) : 2052.26 Transmembrane helix 17 from 323 (in) to 338 (out) : 817.238 Transmembrane helix 18 from 342 (out) to 357 (in) : 1195.44 Transmembrane helix 19 from 361 (in) to 376 (out) : 989.417 Transmembrane helix 20 from 380 (out) to 395 (in) : 3970.51 Transmembrane helix 21 from 399 (in) to 414 (out) : -556.937 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 87 (out) : 2770.33 Transmembrane helix 5 from 91 (out) to 106 (in) : 2213.63 Transmembrane helix 6 from 110 (in) to 125 (out) : 482.734 Transmembrane helix 7 from 130 (out) to 145 (in) : 1658.07 Transmembrane helix 8 from 149 (in) to 164 (out) : 1233.97 Transmembrane helix 9 from 168 (out) to 183 (in) : 3315.19 Transmembrane helix 10 from 191 (in) to 206 (out) : -855.405 Transmembrane helix 11 from 210 (out) to 225 (in) : 2772.57 Transmembrane helix 12 from 229 (in) to 244 (out) : 513.059 Transmembrane helix 13 from 248 (out) to 263 (in) : 2219.73 Transmembrane helix 14 from 267 (in) to 282 (out) : 599.412 Transmembrane helix 15 from 286 (out) to 301 (in) : 3123.42 Transmembrane helix 16 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 17 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 18 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 19 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 20 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 8 from 160 (out) to 178 (in) : 4394.48 Transmembrane helix 9 from 182 (in) to 197 (out) : -1002.55 Transmembrane helix 10 from 201 (out) to 216 (in) : 625.874 Transmembrane helix 11 from 220 (in) to 235 (out) : 2504.27 Transmembrane helix 12 from 239 (out) to 254 (in) : 205.878 Transmembrane helix 13 from 258 (in) to 273 (out) : 2556.82 Transmembrane helix 14 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 15 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 16 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 17 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 18 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 19 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 87 (out) : 2770.33 Transmembrane helix 5 from 91 (out) to 106 (in) : 2213.63 Transmembrane helix 6 from 110 (in) to 125 (out) : 482.734 Transmembrane helix 7 from 136 (out) to 154 (in) : 2949.89 Transmembrane helix 8 from 160 (in) to 180 (out) : 4457.98 Transmembrane helix 9 from 191 (out) to 206 (in) : -844.695 Transmembrane helix 10 from 210 (in) to 231 (out) : 3716.46 Transmembrane helix 11 from 235 (out) to 250 (in) : -98.664 Transmembrane helix 12 from 254 (in) to 271 (out) : 3169.66 Transmembrane helix 13 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 14 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 15 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 16 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 17 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 18 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 5 (in) to 26 (out) : 2545.57 Transmembrane helix 2 from 30 (out) to 45 (in) : 689.493 Transmembrane helix 3 from 49 (in) to 64 (out) : 1255.98 Transmembrane helix 4 from 71 (out) to 87 (in) : 2741.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 2192.97 Transmembrane helix 6 from 110 (out) to 125 (in) : 492.166 Transmembrane helix 7 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 8 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 9 from 210 (in) to 231 (out) : 3716.46 Transmembrane helix 10 from 235 (out) to 250 (in) : -98.664 Transmembrane helix 11 from 254 (in) to 271 (out) : 3169.66 Transmembrane helix 12 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 13 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 14 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 15 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 16 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 17 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 5 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 6 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 7 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 8 from 210 (in) to 231 (out) : 3716.46 Transmembrane helix 9 from 235 (out) to 250 (in) : -98.664 Transmembrane helix 10 from 254 (in) to 271 (out) : 3169.66 Transmembrane helix 11 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 12 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 13 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 14 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 15 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 16 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 4 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 5 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 6 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 7 from 210 (in) to 231 (out) : 3716.46 Transmembrane helix 8 from 235 (out) to 250 (in) : -98.664 Transmembrane helix 9 from 254 (in) to 271 (out) : 3169.66 Transmembrane helix 10 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 11 from 305 (in) to 320 (out) : 2278.52 Transmembrane helix 12 from 324 (out) to 339 (in) : 633.187 Transmembrane helix 13 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 14 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 15 from 381 (in) to 401 (out) : 5065.77 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 5 (out) to 23 (in) : 2265.47 Transmembrane helix 2 from 27 (in) to 42 (out) : 422.985 Transmembrane helix 3 from 46 (out) to 61 (in) : 1784.81 Transmembrane helix 4 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 5 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 6 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 7 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 8 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 9 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 10 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 11 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 12 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 13 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 14 from 381 (in) to 401 (out) : 5065.77 Score = 40.156090 Processing 13 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 71 (in) to 88 (out) : 2802.23 Transmembrane helix 4 from 93 (out) to 117 (in) : 3332.15 Transmembrane helix 5 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 6 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 7 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 8 from 249 (out) to 271 (in) : 3374.09 Transmembrane helix 9 from 281 (in) to 301 (out) : 3732.75 Transmembrane helix 10 from 307 (out) to 330 (in) : 3570.57 Transmembrane helix 11 from 343 (in) to 358 (out) : 1368.53 Transmembrane helix 12 from 362 (out) to 377 (in) : 864.387 Transmembrane helix 13 from 381 (in) to 401 (out) : 5065.77 Score = 40.789066 Processing 12 helices: Transmembrane helix 1 from 5 (out) to 29 (in) : 2589.79 Transmembrane helix 2 from 39 (in) to 61 (out) : 2451.87 Transmembrane helix 3 from 71 (out) to 88 (in) : 2767.77 Transmembrane helix 4 from 93 (in) to 117 (out) : 3294.25 Transmembrane helix 5 from 136 (out) to 154 (in) : 2949.89 Transmembrane helix 6 from 160 (in) to 180 (out) : 4457.98 Transmembrane helix 7 from 210 (out) to 235 (in) : 3804.97 Transmembrane helix 8 from 249 (in) to 271 (out) : 3329.91 Transmembrane helix 9 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 10 from 307 (in) to 330 (out) : 3536.63 Transmembrane helix 11 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 12 from 378 (in) to 401 (out) : 5081.12 Score = 40.333519 Processing 11 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 4 from 136 (out) to 154 (in) : 2949.89 Transmembrane helix 5 from 160 (in) to 180 (out) : 4457.98 Transmembrane helix 6 from 210 (out) to 235 (in) : 3804.97 Transmembrane helix 7 from 249 (in) to 271 (out) : 3329.91 Transmembrane helix 8 from 281 (out) to 301 (in) : 3657.45 Transmembrane helix 9 from 307 (in) to 330 (out) : 3536.63 Transmembrane helix 10 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 11 from 378 (in) to 401 (out) : 5081.12 Score = 38.827480 Processing 10 helices: Transmembrane helix 1 from 5 (out) to 29 (in) : 2589.79 Transmembrane helix 2 from 39 (in) to 61 (out) : 2451.87 Transmembrane helix 3 from 76 (out) to 106 (in) : 4406.67 Transmembrane helix 4 from 136 (in) to 154 (out) : 3001.91 Transmembrane helix 5 from 160 (out) to 180 (in) : 4521.42 Transmembrane helix 6 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 7 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 8 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 9 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 10 from 378 (in) to 401 (out) : 5081.12 Score = 36.865654 Processing 9 helices: Transmembrane helix 1 from 5 (in) to 29 (out) : 2614.41 Transmembrane helix 2 from 39 (out) to 61 (in) : 2471.33 Transmembrane helix 3 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 4 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 5 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 6 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 7 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 8 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 9 from 378 (in) to 401 (out) : 5081.12 Score = 34.660393 Processing 8 helices: Transmembrane helix 1 from 16 (out) to 46 (in) : 2601.02 Transmembrane helix 2 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 3 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 4 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 5 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 6 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 7 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 8 from 378 (in) to 401 (out) : 5081.12 Score = 32.166386 Processing 7 helices: Transmembrane helix 1 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 2 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 3 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 4 from 256 (out) to 286 (in) : 3688.29 Transmembrane helix 5 from 290 (in) to 320 (out) : 4449.34 Transmembrane helix 6 from 346 (out) to 369 (in) : 2699.69 Transmembrane helix 7 from 378 (in) to 401 (out) : 5081.12 Score = 29.534903 Processing 6 helices: Transmembrane helix 1 from 76 (out) to 106 (in) : 4406.67 Transmembrane helix 2 from 145 (in) to 175 (out) : 5161.22 Transmembrane helix 3 from 210 (out) to 235 (in) : 3804.97 Transmembrane helix 4 from 256 (in) to 286 (out) : 3616.51 Transmembrane helix 5 from 290 (out) to 320 (in) : 4403.46 Transmembrane helix 6 from 382 (in) to 401 (out) : 5054.5 Score = 26.250681 Processing 5 helices: Transmembrane helix 1 from 75 (in) to 105 (out) : 4420.3 Transmembrane helix 2 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 3 from 210 (in) to 235 (out) : 3881.03 Transmembrane helix 4 from 285 (out) to 315 (in) : 4614.29 Transmembrane helix 5 from 379 (in) to 401 (out) : 5081.15 Score = 23.272751 Processing 4 helices: Transmembrane helix 1 from 76 (out) to 106 (in) : 4406.67 Transmembrane helix 2 from 145 (in) to 175 (out) : 5161.22 Transmembrane helix 3 from 286 (out) to 316 (in) : 4614.33 Transmembrane helix 4 from 379 (in) to 401 (out) : 5081.15 Score = 19.223284 Processing 3 helices: Transmembrane helix 1 from 145 (in) to 175 (out) : 5161.22 Transmembrane helix 2 from 286 (out) to 316 (in) : 4614.33 Transmembrane helix 3 from 379 (in) to 401 (out) : 5081.15 Score = 14.812860 Processing 2 helices: Transmembrane helix 1 from 145 (out) to 175 (in) : 5197.58 Transmembrane helix 2 from 379 (in) to 401 (out) : 5081.15 Score = 10.234889 Summary of topology analysis: 1 helix (+) : Score = 5.16122 1 helix (-) : Score = 5.19758 2 helices (+) : Score = 10.3561 2 helices (-) : Score = 10.2349 3 helices (+) : Score = 14.8129 3 helices (-) : Score = 15.1378 4 helices (+) : Score = 19.556 4 helices (-) : Score = 19.2233 5 helices (+) : Score = 23.2728 5 helices (-) : Score = 23.1943 6 helices (+) : Score = 27.0579 6 helices (-) : Score = 26.2507 7 helices (+) : Score = 29.5349 7 helices (-) : Score = 29.6893 8 helices (+) : Score = 32.2652 8 helices (-) : Score = 32.1664 9 helices (+) : Score = 34.6604 9 helices (-) : Score = 34.8967 10 helices (+) : Score = 37.3907 10 helices (-) : Score = 36.8657 11 helices (+) : Score = 38.8275 11 helices (-) : Score = 39.596 12 helices (+) : Score = 41.459 12 helices (-) : Score = 40.3335 13 helices (+) : Score = 40.7891 13 helices (-) : Score = 40.826 14 helices (+) : Score = 40.2456 14 helices (-) : Score = 40.1561 15 helices (+) : Score = -100000 15 helices (-) : Score = 39.6126 16 helices (+) : Score = 38.8747 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.82 Topology: 5-29,39-61,71-88,93-117,136-154,160-180,210-235,249-271,281-301,307-330,346-369,379-401 Re-entrant helices: Not detected. Helix count: 12 N-terminal: in Score: 41.459