BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (213 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P76543 Uncharacterized protein yffL n=3 Tax=Escherichia...   444   e-124

>UniRef50_P76543 Uncharacterized protein yffL n=3 Tax=Escherichia coli K-12
           RepID=YFFL_ECOLI
          Length = 213

 Score =  444 bits (1143), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 213/213 (100%), Positives = 213/213 (100%)

Query: 1   MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI 60
           MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI
Sbjct: 1   MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI 60

Query: 61  NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY 120
           NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
Sbjct: 61  NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY 120

Query: 121 ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN 180
           ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN
Sbjct: 121 ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN 180

Query: 181 HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK 213
           HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK
Sbjct: 181 HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK 213


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76543 Uncharacterized protein yffL n=3 Tax=Escherichia...   429   e-119

Sequences not found previously or not previously below threshold:

UniRef50_Q96JM2 Zinc finger protein 462 n=34 Tax=Eukaryota RepID...    41   0.025
UniRef50_Q5T0T3 Zinc finger protein 462 (Fragment) n=12 Tax=Euth...    40   0.048


CONVERGED!
>UniRef50_P76543 Uncharacterized protein yffL n=3 Tax=Escherichia coli K-12
           RepID=YFFL_ECOLI
          Length = 213

 Score =  429 bits (1104), Expect = e-119,   Method: Composition-based stats.
 Identities = 213/213 (100%), Positives = 213/213 (100%)

Query: 1   MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI 60
           MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI
Sbjct: 1   MFATKDPEFENRINTNKSPRNAATCRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKI 60

Query: 61  NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY 120
           NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
Sbjct: 61  NELRNKAPKNEETKHEEHTPDNHEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY 120

Query: 121 ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN 180
           ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN
Sbjct: 121 ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRLVN 180

Query: 181 HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK 213
           HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK
Sbjct: 181 HFKLVERTEGYIPGSAERECNEYQLLFKPYNMK 213


>UniRef50_Q96JM2 Zinc finger protein 462 n=34 Tax=Eukaryota RepID=ZN462_HUMAN
          Length = 2506

 Score = 41.2 bits (95), Expect = 0.025,   Method: Composition-based stats.
 Identities = 33/123 (26%), Positives = 54/123 (43%), Gaps = 6/123 (4%)

Query: 25   CRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKINELRNKAPK--NEETKHEEHTPDN 82
            CR +  K  +G     D       ++  ++Q + K NEL+    +    ETKH E   D+
Sbjct: 2287 CRSKLSKYLQGVVFRCDKCTFTCSSDESLQQHIEKHNELKPYKCQLCYYETKHTEEL-DS 2345

Query: 83   HEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLYERWEKEHTVNDEAVLRALCYFA 142
            H   +H  ++  E   R N   LD L ++ E +     +  +KE  +N +A  R L  F+
Sbjct: 2346 HLRDEHKVSRNFELVGRVN---LDQLEQMKEKMESSSSDDEDKEEEMNSKAEDRELMRFS 2402

Query: 143  GTG 145
              G
Sbjct: 2403 DHG 2405


>UniRef50_Q5T0T3 Zinc finger protein 462 (Fragment) n=12 Tax=Eutheria
           RepID=Q5T0T3_HUMAN
          Length = 931

 Score = 40.1 bits (92), Expect = 0.048,   Method: Composition-based stats.
 Identities = 33/123 (26%), Positives = 54/123 (43%), Gaps = 6/123 (4%)

Query: 25  CRGRYEKQAKGEFLMSDMLAVEQETNNDVRQFLNKINELRNKAPK--NEETKHEEHTPDN 82
           CR +  K  +G     D       ++  ++Q + K NEL+    +    ETKH E   D+
Sbjct: 712 CRSKLSKYLQGVVFRCDKCTFTCSSDESLQQHIEKHNELKPYKCQLCYYETKHTEEL-DS 770

Query: 83  HEETDHHEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLYERWEKEHTVNDEAVLRALCYFA 142
           H   +H  ++  E   R N   LD L ++ E +     +  +KE  +N +A  R L  F+
Sbjct: 771 HLRDEHKVSRNFELVGRVN---LDQLEQMKEKMESSSSDDEDKEEEMNSKAEDRELMRFS 827

Query: 143 GTG 145
             G
Sbjct: 828 DHG 830


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.131    0.393 

Lambda     K      H
   0.267   0.0409    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 846,799,102
Number of Sequences: 3077464
Number of extensions: 33312697
Number of successful extensions: 145481
Number of sequences better than 1.0e-01: 4
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 145387
Number of HSP's gapped (non-prelim): 66
length of query: 213
length of database: 1,040,396,356
effective HSP length: 123
effective length of query: 90
effective length of database: 661,868,284
effective search space: 59568145560
effective search space used: 59568145560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 90 (39.3 bits)