Processing 1 helix: Transmembrane helix 1 from 62 (in) to 85 (out) : 3430.25 Score = 3.430254 Processing 1 helix: Transmembrane helix 1 from 61 (out) to 85 (in) : 3497.94 Score = 3.521318 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 942.969 Transmembrane helix 2 from 23 (out) to 38 (in) : 1507.68 Transmembrane helix 3 from 42 (in) to 57 (out) : 233.101 Transmembrane helix 4 from 61 (out) to 77 (in) : 2994.92 Transmembrane helix 5 from 81 (in) to 96 (out) : 190.168 Transmembrane helix 6 from 100 (out) to 115 (in) : 1565.81 Transmembrane helix 7 from 119 (in) to 134 (out) : -283.5 Transmembrane helix 8 from 138 (out) to 153 (in) : -167.937 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 964.531 Transmembrane helix 2 from 23 (in) to 38 (out) : 1524.72 Transmembrane helix 3 from 42 (out) to 57 (in) : 247.599 Transmembrane helix 4 from 61 (in) to 84 (out) : 3430.9 Transmembrane helix 5 from 88 (out) to 103 (in) : 297.064 Transmembrane helix 6 from 107 (in) to 123 (out) : 1438.27 Transmembrane helix 7 from 137 (out) to 152 (in) : -162.463 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 21 (out) : 964.719 Transmembrane helix 2 from 25 (out) to 48 (in) : 2311.25 Transmembrane helix 3 from 61 (in) to 84 (out) : 3430.9 Transmembrane helix 4 from 88 (out) to 103 (in) : 297.064 Transmembrane helix 5 from 107 (in) to 123 (out) : 1438.27 Transmembrane helix 6 from 137 (out) to 152 (in) : -162.463 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 21 (in) : 997.581 Transmembrane helix 2 from 25 (in) to 48 (out) : 2365.15 Transmembrane helix 3 from 61 (out) to 85 (in) : 3497.94 Transmembrane helix 4 from 97 (in) to 123 (out) : 1967.73 Transmembrane helix 5 from 137 (out) to 152 (in) : -162.463 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 21 (out) : 964.719 Transmembrane helix 2 from 25 (out) to 48 (in) : 2311.25 Transmembrane helix 3 from 61 (in) to 85 (out) : 3442.46 Transmembrane helix 4 from 96 (out) to 123 (in) : 1923.9 Score = 8.550767 Processing 3 helices: Transmembrane helix 1 from 14 (out) to 44 (in) : 2596.47 Transmembrane helix 2 from 61 (in) to 85 (out) : 3442.46 Transmembrane helix 3 from 96 (out) to 123 (in) : 1923.9 Score = 7.891112 Processing 2 helices: Transmembrane helix 1 from 14 (in) to 44 (out) : 2625.03 Transmembrane helix 2 from 61 (out) to 85 (in) : 3497.94 Score = 6.128984 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 964.531 Transmembrane helix 2 from 23 (in) to 38 (out) : 1524.72 Transmembrane helix 3 from 42 (out) to 57 (in) : 247.599 Transmembrane helix 4 from 61 (in) to 77 (out) : 2946.88 Transmembrane helix 5 from 81 (out) to 96 (in) : 180.132 Transmembrane helix 6 from 100 (in) to 115 (out) : 1587.59 Transmembrane helix 7 from 119 (out) to 134 (in) : -283.1 Transmembrane helix 8 from 138 (in) to 153 (out) : -171.163 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 942.969 Transmembrane helix 2 from 23 (out) to 38 (in) : 1507.68 Transmembrane helix 3 from 42 (in) to 57 (out) : 233.101 Transmembrane helix 4 from 61 (out) to 85 (in) : 3497.94 Transmembrane helix 5 from 89 (in) to 104 (out) : 391.131 Transmembrane helix 6 from 108 (out) to 123 (in) : 1319.89 Transmembrane helix 7 from 137 (in) to 152 (out) : -165.037 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 21 (in) : 997.581 Transmembrane helix 2 from 25 (in) to 48 (out) : 2365.15 Transmembrane helix 3 from 61 (out) to 85 (in) : 3497.94 Transmembrane helix 4 from 89 (in) to 104 (out) : 391.131 Transmembrane helix 5 from 108 (out) to 123 (in) : 1319.89 Transmembrane helix 6 from 137 (in) to 152 (out) : -165.037 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 21 (out) : 964.719 Transmembrane helix 2 from 25 (out) to 48 (in) : 2311.25 Transmembrane helix 3 from 61 (in) to 84 (out) : 3430.9 Transmembrane helix 4 from 88 (out) to 103 (in) : 297.064 Transmembrane helix 5 from 107 (in) to 123 (out) : 1438.27 Score = 8.447365 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 21 (in) : 997.581 Transmembrane helix 2 from 25 (in) to 48 (out) : 2365.15 Transmembrane helix 3 from 61 (out) to 85 (in) : 3497.94 Transmembrane helix 4 from 97 (in) to 123 (out) : 1967.73 Score = 8.921624 Processing 3 helices: Transmembrane helix 1 from 14 (in) to 44 (out) : 2625.03 Transmembrane helix 2 from 61 (out) to 85 (in) : 3497.94 Transmembrane helix 3 from 97 (in) to 123 (out) : 1967.73 Score = 8.164079 Processing 2 helices: Transmembrane helix 1 from 14 (out) to 44 (in) : 2596.47 Transmembrane helix 2 from 61 (in) to 85 (out) : 3442.46 Score = 6.032916 Summary of topology analysis: 1 helix (+) : Score = 3.43025 1 helix (-) : Score = 3.52132 2 helices (+) : Score = 6.12898 2 helices (-) : Score = 6.03292 3 helices (+) : Score = 8.16408 3 helices (-) : Score = 7.89111 4 helices (+) : Score = 8.55077 4 helices (-) : Score = 8.92162 5 helices (+) : Score = 8.44736 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 4-21,25-48,61-85,97-123 Re-entrant helices: Not detected. Helix count: 4 N-terminal: out Score: 8.92162