Processing 1 helix: Transmembrane helix 1 from 208 (in) to 223 (out) : -1.513 Score = -0.001513 Processing 1 helix: Transmembrane helix 1 from 208 (out) to 223 (in) : -4.387 Score = -0.004387 Processing 15 helices: Transmembrane helix 1 from 11 (in) to 26 (out) : -9522.34 Transmembrane helix 2 from 30 (out) to 45 (in) : -297.691 Transmembrane helix 3 from 49 (in) to 64 (out) : -655.078 Transmembrane helix 4 from 68 (out) to 83 (in) : -333.812 Transmembrane helix 5 from 87 (in) to 102 (out) : -208.764 Transmembrane helix 6 from 106 (out) to 121 (in) : -356.725 Transmembrane helix 7 from 125 (in) to 140 (out) : -211.869 Transmembrane helix 8 from 144 (out) to 159 (in) : -392.629 Transmembrane helix 9 from 163 (in) to 178 (out) : -701.536 Transmembrane helix 10 from 182 (out) to 197 (in) : -756.449 Transmembrane helix 11 from 201 (in) to 216 (out) : -123.344 Transmembrane helix 12 from 220 (out) to 235 (in) : -269.362 Transmembrane helix 13 from 239 (in) to 254 (out) : -280.739 Transmembrane helix 14 from 258 (out) to 273 (in) : -108.928 Transmembrane helix 15 from 277 (in) to 292 (out) : -698.738 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 64 (out) to 79 (in) : -508.769 Transmembrane helix 4 from 83 (in) to 98 (out) : -132.44 Transmembrane helix 5 from 102 (out) to 117 (in) : -298.922 Transmembrane helix 6 from 121 (in) to 136 (out) : -302.315 Transmembrane helix 7 from 140 (out) to 155 (in) : -327.384 Transmembrane helix 8 from 159 (in) to 174 (out) : -621.466 Transmembrane helix 9 from 182 (out) to 197 (in) : -756.449 Transmembrane helix 10 from 201 (in) to 216 (out) : -123.344 Transmembrane helix 11 from 220 (out) to 235 (in) : -269.362 Transmembrane helix 12 from 239 (in) to 254 (out) : -280.739 Transmembrane helix 13 from 258 (out) to 273 (in) : -108.928 Transmembrane helix 14 from 277 (in) to 292 (out) : -698.738 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -369.782 Transmembrane helix 2 from 38 (out) to 53 (in) : -407.147 Transmembrane helix 3 from 60 (in) to 75 (out) : -631.113 Transmembrane helix 4 from 79 (out) to 94 (in) : -92.497 Transmembrane helix 5 from 98 (in) to 113 (out) : -249.915 Transmembrane helix 6 from 117 (out) to 132 (in) : -395.628 Transmembrane helix 7 from 136 (in) to 151 (out) : -267.523 Transmembrane helix 8 from 155 (out) to 170 (in) : -554.068 Transmembrane helix 9 from 193 (in) to 208 (out) : -383.185 Transmembrane helix 10 from 212 (out) to 227 (in) : -59.435 Transmembrane helix 11 from 231 (in) to 246 (out) : -289.265 Transmembrane helix 12 from 250 (out) to 265 (in) : -192.351 Transmembrane helix 13 from 269 (in) to 284 (out) : -466.193 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 73 (out) to 88 (in) : -124.629 Transmembrane helix 4 from 92 (in) to 107 (out) : -287.877 Transmembrane helix 5 from 111 (out) to 126 (in) : -394.532 Transmembrane helix 6 from 130 (in) to 145 (out) : -199.264 Transmembrane helix 7 from 149 (out) to 164 (in) : -448.789 Transmembrane helix 8 from 193 (in) to 208 (out) : -383.185 Transmembrane helix 9 from 212 (out) to 227 (in) : -59.435 Transmembrane helix 10 from 231 (in) to 246 (out) : -289.265 Transmembrane helix 11 from 250 (out) to 265 (in) : -192.351 Transmembrane helix 12 from 269 (in) to 284 (out) : -466.193 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -369.782 Transmembrane helix 2 from 38 (out) to 53 (in) : -407.147 Transmembrane helix 3 from 73 (in) to 88 (out) : -142.071 Transmembrane helix 4 from 92 (out) to 107 (in) : -281.623 Transmembrane helix 5 from 111 (in) to 126 (out) : -400.668 Transmembrane helix 6 from 130 (out) to 145 (in) : -206.036 Transmembrane helix 7 from 149 (in) to 164 (out) : -445.511 Transmembrane helix 8 from 205 (out) to 220 (in) : -44.341 Transmembrane helix 9 from 224 (in) to 239 (out) : -302.596 Transmembrane helix 10 from 243 (out) to 258 (in) : -279.691 Transmembrane helix 11 from 262 (in) to 277 (out) : -150.905 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 4 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 5 from 124 (out) to 139 (in) : -222.601 Transmembrane helix 6 from 143 (in) to 158 (out) : -386.243 Transmembrane helix 7 from 205 (out) to 220 (in) : -44.341 Transmembrane helix 8 from 224 (in) to 239 (out) : -302.596 Transmembrane helix 9 from 243 (out) to 258 (in) : -279.691 Transmembrane helix 10 from 262 (in) to 277 (out) : -150.905 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -293.409 Transmembrane helix 2 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 3 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 4 from 124 (out) to 139 (in) : -222.601 Transmembrane helix 5 from 143 (in) to 158 (out) : -386.243 Transmembrane helix 6 from 205 (out) to 220 (in) : -44.341 Transmembrane helix 7 from 224 (in) to 239 (out) : -302.596 Transmembrane helix 8 from 243 (out) to 258 (in) : -279.691 Transmembrane helix 9 from 262 (in) to 277 (out) : -150.905 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -297.691 Transmembrane helix 2 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 3 from 98 (out) to 113 (in) : -247.685 Transmembrane helix 4 from 130 (in) to 145 (out) : -199.264 Transmembrane helix 5 from 205 (out) to 220 (in) : -44.341 Transmembrane helix 6 from 224 (in) to 239 (out) : -302.596 Transmembrane helix 7 from 243 (out) to 258 (in) : -279.691 Transmembrane helix 8 from 262 (in) to 277 (out) : -150.905 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -293.409 Transmembrane helix 2 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 3 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 4 from 129 (out) to 144 (in) : -204.765 Transmembrane helix 5 from 208 (in) to 223 (out) : -1.513 Transmembrane helix 6 from 237 (out) to 252 (in) : -282.111 Transmembrane helix 7 from 256 (in) to 271 (out) : -112.957 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -297.691 Transmembrane helix 2 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 3 from 98 (out) to 113 (in) : -247.685 Transmembrane helix 4 from 131 (in) to 146 (out) : -206.138 Transmembrane helix 5 from 207 (out) to 222 (in) : -13.688 Transmembrane helix 6 from 256 (in) to 271 (out) : -112.957 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 2 from 98 (out) to 113 (in) : -247.685 Transmembrane helix 3 from 131 (in) to 146 (out) : -206.138 Transmembrane helix 4 from 207 (out) to 222 (in) : -13.688 Transmembrane helix 5 from 256 (in) to 271 (out) : -112.957 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 2 from 131 (in) to 146 (out) : -206.138 Transmembrane helix 3 from 207 (out) to 222 (in) : -13.688 Transmembrane helix 4 from 256 (in) to 271 (out) : -112.957 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -86.634 Transmembrane helix 2 from 208 (out) to 223 (in) : -4.387 Transmembrane helix 3 from 256 (in) to 271 (out) : -112.957 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 2 from 208 (in) to 223 (out) : -1.513 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 11 (out) to 26 (in) : -9480.96 Transmembrane helix 2 from 30 (in) to 45 (out) : -293.409 Transmembrane helix 3 from 49 (out) to 64 (in) : -644.522 Transmembrane helix 4 from 68 (in) to 83 (out) : -341.888 Transmembrane helix 5 from 87 (out) to 102 (in) : -215.836 Transmembrane helix 6 from 106 (in) to 121 (out) : -351.475 Transmembrane helix 7 from 125 (out) to 140 (in) : -212.531 Transmembrane helix 8 from 144 (in) to 159 (out) : -400.671 Transmembrane helix 9 from 163 (out) to 178 (in) : -709.564 Transmembrane helix 10 from 182 (in) to 197 (out) : -754.551 Transmembrane helix 11 from 201 (out) to 216 (in) : -109.856 Transmembrane helix 12 from 220 (in) to 235 (out) : -275.138 Transmembrane helix 13 from 239 (out) to 254 (in) : -295.861 Transmembrane helix 14 from 258 (in) to 273 (out) : -116.572 Transmembrane helix 15 from 277 (out) to 292 (in) : -689.662 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -369.782 Transmembrane helix 2 from 38 (out) to 53 (in) : -407.147 Transmembrane helix 3 from 60 (in) to 75 (out) : -631.113 Transmembrane helix 4 from 79 (out) to 94 (in) : -92.497 Transmembrane helix 5 from 98 (in) to 113 (out) : -249.915 Transmembrane helix 6 from 117 (out) to 132 (in) : -395.628 Transmembrane helix 7 from 136 (in) to 151 (out) : -267.523 Transmembrane helix 8 from 155 (out) to 170 (in) : -554.068 Transmembrane helix 9 from 182 (in) to 197 (out) : -754.551 Transmembrane helix 10 from 201 (out) to 216 (in) : -109.856 Transmembrane helix 11 from 220 (in) to 235 (out) : -275.138 Transmembrane helix 12 from 239 (out) to 254 (in) : -295.861 Transmembrane helix 13 from 258 (in) to 273 (out) : -116.572 Transmembrane helix 14 from 277 (out) to 292 (in) : -689.662 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 64 (out) to 79 (in) : -508.769 Transmembrane helix 4 from 83 (in) to 98 (out) : -132.44 Transmembrane helix 5 from 102 (out) to 117 (in) : -298.922 Transmembrane helix 6 from 121 (in) to 136 (out) : -302.315 Transmembrane helix 7 from 140 (out) to 155 (in) : -327.384 Transmembrane helix 8 from 159 (in) to 174 (out) : -621.466 Transmembrane helix 9 from 193 (out) to 208 (in) : -379.015 Transmembrane helix 10 from 212 (in) to 227 (out) : -61.565 Transmembrane helix 11 from 231 (out) to 246 (in) : -305.735 Transmembrane helix 12 from 250 (in) to 265 (out) : -199.649 Transmembrane helix 13 from 269 (out) to 284 (in) : -465.307 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -369.782 Transmembrane helix 2 from 38 (out) to 53 (in) : -407.147 Transmembrane helix 3 from 73 (in) to 88 (out) : -142.071 Transmembrane helix 4 from 92 (out) to 107 (in) : -281.623 Transmembrane helix 5 from 111 (in) to 126 (out) : -400.668 Transmembrane helix 6 from 130 (out) to 145 (in) : -206.036 Transmembrane helix 7 from 149 (in) to 164 (out) : -445.511 Transmembrane helix 8 from 193 (out) to 208 (in) : -379.015 Transmembrane helix 9 from 212 (in) to 227 (out) : -61.565 Transmembrane helix 10 from 231 (out) to 246 (in) : -305.735 Transmembrane helix 11 from 250 (in) to 265 (out) : -199.649 Transmembrane helix 12 from 269 (out) to 284 (in) : -465.307 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 73 (out) to 88 (in) : -124.629 Transmembrane helix 4 from 92 (in) to 107 (out) : -287.877 Transmembrane helix 5 from 111 (out) to 126 (in) : -394.532 Transmembrane helix 6 from 130 (in) to 145 (out) : -199.264 Transmembrane helix 7 from 149 (out) to 164 (in) : -448.789 Transmembrane helix 8 from 196 (in) to 211 (out) : -265.425 Transmembrane helix 9 from 215 (out) to 230 (in) : -157.229 Transmembrane helix 10 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 11 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -293.409 Transmembrane helix 2 from 73 (out) to 88 (in) : -124.629 Transmembrane helix 3 from 92 (in) to 107 (out) : -287.877 Transmembrane helix 4 from 111 (out) to 126 (in) : -394.532 Transmembrane helix 5 from 130 (in) to 145 (out) : -199.264 Transmembrane helix 6 from 149 (out) to 164 (in) : -448.789 Transmembrane helix 7 from 196 (in) to 211 (out) : -265.425 Transmembrane helix 8 from 215 (out) to 230 (in) : -157.229 Transmembrane helix 9 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 10 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -362.418 Transmembrane helix 2 from 38 (in) to 53 (out) : -384.553 Transmembrane helix 3 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 4 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 5 from 124 (out) to 139 (in) : -222.601 Transmembrane helix 6 from 143 (in) to 158 (out) : -386.243 Transmembrane helix 7 from 208 (out) to 223 (in) : -4.387 Transmembrane helix 8 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 9 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -293.409 Transmembrane helix 2 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 3 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 4 from 124 (out) to 139 (in) : -222.601 Transmembrane helix 5 from 143 (in) to 158 (out) : -386.243 Transmembrane helix 6 from 208 (out) to 223 (in) : -4.387 Transmembrane helix 7 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 8 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -297.691 Transmembrane helix 2 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 3 from 98 (out) to 113 (in) : -247.685 Transmembrane helix 4 from 131 (in) to 146 (out) : -206.138 Transmembrane helix 5 from 207 (out) to 222 (in) : -13.688 Transmembrane helix 6 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 7 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 2 from 98 (out) to 113 (in) : -247.685 Transmembrane helix 3 from 131 (in) to 146 (out) : -206.138 Transmembrane helix 4 from 207 (out) to 222 (in) : -13.688 Transmembrane helix 5 from 234 (in) to 249 (out) : -257.375 Transmembrane helix 6 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 2 from 97 (in) to 112 (out) : -251.442 Transmembrane helix 3 from 129 (out) to 144 (in) : -204.765 Transmembrane helix 4 from 208 (in) to 223 (out) : -1.513 Transmembrane helix 5 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 77 (in) to 92 (out) : -87.393 Transmembrane helix 2 from 129 (out) to 144 (in) : -204.765 Transmembrane helix 3 from 208 (in) to 223 (out) : -1.513 Transmembrane helix 4 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 77 (out) to 92 (in) : -76.707 Transmembrane helix 2 from 208 (in) to 223 (out) : -1.513 Transmembrane helix 3 from 256 (out) to 271 (in) : -100.143 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -86.634 Transmembrane helix 2 from 208 (out) to 223 (in) : -4.387 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.001513 1 helix (-) : Score = -0.004387 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 43.79 Topology: 208-223 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.001513