BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (54 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q0TFN3 Uncharacterized protein yojO n=41 Tax=Enterobact...   108   7e-23
UniRef50_A8AE09 Putative uncharacterized protein n=7 Tax=Enterob...    42   0.005

>UniRef50_Q0TFN3 Uncharacterized protein yojO n=41 Tax=Enterobacteriaceae
          RepID=YOJO_ECOL5
          Length = 54

 Score =  108 bits (269), Expect = 7e-23,   Method: Compositional matrix adjust.
 Identities = 51/54 (94%), Positives = 53/54 (98%)

Query: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAIDSPEIFLLVCSLIRI 54
          MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYK+IYIAI+SPEIFLLVC LIRI
Sbjct: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEIFLLVCFLIRI 54


>UniRef50_A8AE09 Putative uncharacterized protein n=7 Tax=Enterobacteriaceae
          RepID=A8AE09_CITK8
          Length = 55

 Score = 42.0 bits (97), Expect = 0.005,   Method: Compositional matrix adjust.
 Identities = 21/38 (55%), Positives = 24/38 (63%)

Query: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIA 38
          +HTPIGV  VAGSKE   +  + AF HIS GY   YI 
Sbjct: 12 VHTPIGVNAVAGSKECGVSVSECAFVHISKGYINKYIT 49


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q0TFN3 Uncharacterized protein yojO n=41 Tax=Enterobact...   103   2e-21

Sequences not found previously or not previously below threshold:

UniRef50_A8AE09 Putative uncharacterized protein n=7 Tax=Enterob...    41   0.013


CONVERGED!
>UniRef50_Q0TFN3 Uncharacterized protein yojO n=41 Tax=Enterobacteriaceae
          RepID=YOJO_ECOL5
          Length = 54

 Score =  103 bits (258), Expect = 2e-21,   Method: Composition-based stats.
 Identities = 51/54 (94%), Positives = 53/54 (98%)

Query: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAIDSPEIFLLVCSLIRI 54
          MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYK+IYIAI+SPEIFLLVC LIRI
Sbjct: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEIFLLVCFLIRI 54


>UniRef50_A8AE09 Putative uncharacterized protein n=7 Tax=Enterobacteriaceae
          RepID=A8AE09_CITK8
          Length = 55

 Score = 40.7 bits (94), Expect = 0.013,   Method: Composition-based stats.
 Identities = 21/37 (56%), Positives = 24/37 (64%)

Query: 1  MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYI 37
          +HTPIGV  VAGSKE   +  + AF HIS GY   YI
Sbjct: 12 VHTPIGVNAVAGSKECGVSVSECAFVHISKGYINKYI 48


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.322    0.147    0.498 

Lambda     K      H
   0.267   0.0444    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 288,745,221
Number of Sequences: 3077464
Number of extensions: 9702529
Number of successful extensions: 27908
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 27904
Number of HSP's gapped (non-prelim): 4
length of query: 54
length of database: 1,040,396,356
effective HSP length: 27
effective length of query: 27
effective length of database: 957,304,828
effective search space: 25847230356
effective search space used: 25847230356
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.3 bits)
S2: 87 (38.0 bits)