Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	F	S	H	R	L	F	L	L	L	I	L	L	L	T	G	A	P	I	L	A	Q	E	P	S	D	V	A	K	N	V	R	M	M	V	S	G	I	V	S	Y	T	R	W	P	A	L	S	G	P	P	K	L	C	I	F	S	S	S
Secondary structure
SS confidence
Disorder		?	?	?	?													?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	F	S	T	A	L	Q	E	N	A	A	T	S	L	P	Y	L	P	V	I	I	H	T	Q	Q	E	A	M	I	S	G	C	N	G	F	Y	F	G	N	E	S	P	T	F	Q	M	E	L	T	E	Q	Y	P	S	K	A	L	L	L	I
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?	?	?	?						?	?	?	?	?	?											?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.
Sequence		A	E	Q	N	T	E	C	I	I	G	S	A	F	C	L	I	I	H	N	N	D	V	R	F	A	V	N	L	D	A	L	S	R	S	G	V	K	V	N	P	D	V	L	M	L	A	R	K	K	N	D	G
Secondary structure
SS confidence
Disorder																					?													?	?	?	?			?							?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2qh8 chain A Region: 136 - 170 Aligned: 35 Modelled: 35 Confidence: 38.9% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved domain protein from vibrio2 cholerae o1 biovar eltor str. n16961 Phyre2 PDB 3lft chain A Region: 136 - 166 Aligned: 31 Modelled: 31 Confidence: 34.6% Identity: 16% PDB header:structure genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: the crystal structure of the abc domain in complex with l-trp from2 streptococcus pneumonia to 1.35a Phyre2 PDB 1iib chain A Region: 52 - 131 Aligned: 79 Modelled: 80 Confidence: 29.9% Identity: 10% Fold: Phosphotyrosine protein phosphatases I-like Superfamily: PTS system IIB component-like Family: PTS system, Lactose/Cellobiose specific IIB subunit Phyre2 PDB 2hjs chain A domain 1 Region: 50 - 126 Aligned: 76 Modelled: 77 Confidence: 26.5% Identity: 11% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain Phyre2 PDB 2ljc chain A Region: 28 - 45 Aligned: 18 Modelled: 18 Confidence: 22.5% Identity: 22% PDB header:transport protein/inhibitor Chain: A: PDB Molecule:m2 protein, bm2 protein chimera; PDBTitle: structure of the influenza am2-bm2 chimeric channel bound to2 rimantadine Phyre2 PDB 1pjq chain A domain 1 Region: 73 - 128 Aligned: 56 Modelled: 56 Confidence: 19.9% Identity: 9% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Siroheme synthase N-terminal domain-like Phyre2 PDB 2hu9 chain B Region: 90 - 101 Aligned: 12 Modelled: 12 Confidence: 19.0% Identity: 25% PDB header:metal transport Chain: B: PDB Molecule:mercuric transport protein periplasmic component; PDBTitle: x-ray structure of the archaeoglobus fulgidus copz n-2 terminal domain Phyre2 PDB 2hwk chain A Region: 106 - 149 Aligned: 42 Modelled: 44 Confidence: 18.3% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:helicase nsp2; PDBTitle: crystal structure of venezuelan equine encephalitis2 alphavirus nsp2 protease domain Phyre2 PDB 2abw chain A domain 1 Region: 50 - 121 Aligned: 72 Modelled: 72 Confidence: 17.8% Identity: 11% Fold: Flavodoxin-like Superfamily: Class I glutamine amidotransferase-like Family: Class I glutamine amidotransferases (GAT) Phyre2 PDB 3cu0 chain A domain 1 Region: 94 - 163 Aligned: 64 Modelled: 70 Confidence: 16.3% Identity: 25% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: 1,3-glucuronyltransferase Phyre2 PDB 1k9v chain F Region: 52 - 121 Aligned: 70 Modelled: 70 Confidence: 15.3% Identity: 6% Fold: Flavodoxin-like Superfamily: Class I glutamine amidotransferase-like Family: Class I glutamine amidotransferases (GAT) Phyre2 PDB 2bs3 chain A Region: 88 - 132 Aligned: 45 Modelled: 45 Confidence: 11.1% Identity: 9% PDB header:oxidoreductase Chain: A: PDB Molecule:quinol-fumarate reductase flavoprotein subunit a; PDBTitle: glu c180 -> gln variant quinol:fumarate reductase from2 wolinella succinogenes Phyre2 PDB 2l2q chain A Region: 52 - 131 Aligned: 79 Modelled: 80 Confidence: 11.1% Identity: 11% PDB header:transferase Chain: A: PDB Molecule:pts system, cellobiose-specific iib component (cela); PDBTitle: solution structure of cellobiose-specific phosphotransferase iib2 component protein from borrelia burgdorferi Phyre2 PDB 1wvh chain A domain 1 Region: 90 - 101 Aligned: 12 Modelled: 12 Confidence: 10.4% Identity: 17% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Phosphotyrosine-binding domain (PTB) Phyre2 PDB 2h1q chain A domain 1 Region: 53 - 127 Aligned: 75 Modelled: 75 Confidence: 10.3% Identity: 13% Fold: PLP-dependent transferase-like Superfamily: Dhaf3308-like Family: Dhaf3308-like Phyre2 PDB 2fwt chain A Region: 90 - 115 Aligned: 26 Modelled: 26 Confidence: 10.0% Identity: 19% PDB header:electron transport Chain: A: PDB Molecule:dhc, diheme cytochrome c; PDBTitle: crystal structure of dhc purified from rhodobacter2 sphaeroides Phyre2 PDB 3c85 chain A Region: 46 - 124 Aligned: 79 Modelled: 76 Confidence: 9.1% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:putative glutathione-regulated potassium-efflux system PDBTitle: crystal structure of trka domain of putative glutathione-regulated2 potassium-efflux kefb from vibrio parahaemolyticus Phyre2 PDB 2ioj chain A domain 1 Region: 95 - 170 Aligned: 61 Modelled: 61 Confidence: 8.9% Identity: 23% Fold: MurF and HprK N-domain-like Superfamily: HprK N-terminal domain-like Family: DRTGG domain Phyre2 PDB 2x77 chain B Region: 34 - 63 Aligned: 29 Modelled: 30 Confidence: 8.0% Identity: 7% PDB header:gtp-binding protein Chain: B: PDB Molecule:adp-ribosylation factor; PDBTitle: crystal structure of leishmania major adp ribosylation2 factor-like 1. Phyre2 PDB 2qn6 chain A domain 3 Region: 45 - 63 Aligned: 19 Modelled: 19 Confidence: 8.0% Identity: 11% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: G proteins Phyre2 1 c2qh8A_ 2 c3lftA_ 3 d1iiba_ 4 d2hjsa1 5 c2ljcA_ 6 d1pjqa1 7 c2hu9B_ 8 c2hwkA_ 9 d2abwa1 10 d3cu0a1 11 d1k9vf_ 12 c2bs3A_ 13 c2l2qA_ 14 d1wvha1 15 d2h1qa1 16 c2fwtA_ 17 c3c85A_ 18 d2ioja1 19 c2x77B_ 20 d2qn6a3 21 22 23 24 25 26 27 28 29 30 31 32 33 34

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2qh8A_ 38.9 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved domain protein from vibrio2 cholerae o1 biovar eltor str. n16961 2 c3lftA_ 34.6 16 PDB header:structure genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: the crystal structure of the abc domain in complex with l-trp from2 streptococcus pneumonia to 1.35a 3 d1iiba_ 29.9 10 Fold:Phosphotyrosine protein phosphatases I-like Superfamily:PTS system IIB component-like Family:PTS system, Lactose/Cellobiose specific IIB subunit 4 d2hjsa1 26.5 11 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 5 c2ljcA_ 22.5 22 PDB header:transport protein/inhibitor Chain: A: PDB Molecule:m2 protein, bm2 protein chimera; PDBTitle: structure of the influenza am2-bm2 chimeric channel bound to2 rimantadine 6 d1pjqa1 19.9 9 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Siroheme synthase N-terminal domain-like 7 c2hu9B_ 19.0 25 PDB header:metal transport Chain: B: PDB Molecule:mercuric transport protein periplasmic component; PDBTitle: x-ray structure of the archaeoglobus fulgidus copz n-2 terminal domain 8 c2hwkA_ 18.3 14 PDB header:hydrolase Chain: A: PDB Molecule:helicase nsp2; PDBTitle: crystal structure of venezuelan equine encephalitis2 alphavirus nsp2 protease domain 9 d2abwa1 17.8 11 Fold:Flavodoxin-like Superfamily:Class I glutamine amidotransferase-like Family:Class I glutamine amidotransferases (GAT) 10 d3cu0a1 16.3 25 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:1,3-glucuronyltransferase 11 d1k9vf_ 15.3 6 Fold:Flavodoxin-like Superfamily:Class I glutamine amidotransferase-like Family:Class I glutamine amidotransferases (GAT) 12 c2bs3A_ 11.1 9 PDB header:oxidoreductase Chain: A: PDB Molecule:quinol-fumarate reductase flavoprotein subunit a; PDBTitle: glu c180 -> gln variant quinol:fumarate reductase from2 wolinella succinogenes 13 c2l2qA_ 11.1 11 PDB header:transferase Chain: A: PDB Molecule:pts system, cellobiose-specific iib component (cela); PDBTitle: solution structure of cellobiose-specific phosphotransferase iib2 component protein from borrelia burgdorferi 14 d1wvha1 10.4 17 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Phosphotyrosine-binding domain (PTB) 15 d2h1qa1 10.3 13 Fold:PLP-dependent transferase-like Superfamily:Dhaf3308-like Family:Dhaf3308-like 16 c2fwtA_ 10.0 19 PDB header:electron transport Chain: A: PDB Molecule:dhc, diheme cytochrome c; PDBTitle: crystal structure of dhc purified from rhodobacter2 sphaeroides 17 c3c85A_ 9.1 16 PDB header:transport protein Chain: A: PDB Molecule:putative glutathione-regulated potassium-efflux system PDBTitle: crystal structure of trka domain of putative glutathione-regulated2 potassium-efflux kefb from vibrio parahaemolyticus 18 d2ioja1 8.9 23 Fold:MurF and HprK N-domain-like Superfamily:HprK N-terminal domain-like Family:DRTGG domain 19 c2x77B_ 8.0 7 PDB header:gtp-binding protein Chain: B: PDB Molecule:adp-ribosylation factor; PDBTitle: crystal structure of leishmania major adp ribosylation2 factor-like 1. 20 d2qn6a3 8.0 11 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:G proteins 21 c3doeA_ not modelled 7.9 8 PDB header:signaling protein/hydrolase Chain: A: PDB Molecule:adp-ribosylation factor-like protein 2; PDBTitle: complex of arl2 and bart, crystal form 1 22 d2imra1 not modelled 7.0 45 Fold:Composite domain of metallo-dependent hydrolases Superfamily:Composite domain of metallo-dependent hydrolases Family:DR0824-like 23 d2gp4a1 not modelled 6.8 15 Fold:The "swivelling" beta/beta/alpha domain Superfamily:LeuD/IlvD-like Family:IlvD/EDD C-terminal domain-like 24 c2d0jD_ not modelled 6.7 22 PDB header:transferase Chain: D: PDB Molecule:galactosylgalactosylxylosylprotein 3-beta- PDBTitle: crystal structure of human glcat-s apo form 25 c2dc1A_ not modelled 6.6 6 PDB header:oxidoreductase Chain: A: PDB Molecule:l-aspartate dehydrogenase; PDBTitle: crystal structure of l-aspartate dehydrogenase from2 hyperthermophilic archaeon archaeoglobus fulgidus 26 c3c1aB_ not modelled 6.3 14 PDB header:oxidoreductase Chain: B: PDB Molecule:putative oxidoreductase; PDBTitle: crystal structure of a putative oxidoreductase (zp_00056571.1) from2 magnetospirillum magnetotacticum ms-1 at 1.85 a resolution 27 c4a1eF_ not modelled 6.2 33 PDB header:ribosome Chain: F: PDB Molecule:rpl7a; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 5s rrna, 5.8s rrna3 and proteins of molecule 1 28 d1cjba_ not modelled 6.2 15 Fold:PRTase-like Superfamily:PRTase-like Family:Phosphoribosyltransferases (PRTases) 29 c1yq4A_ not modelled 5.8 12 PDB header:oxidoreductase Chain: A: PDB Molecule:succinate dehydrogenase flavoprotein subunit; PDBTitle: avian respiratory complex ii with 3-nitropropionate and ubiquinone 30 c3db0B_ not modelled 5.8 7 PDB header:oxidoreductase Chain: B: PDB Molecule:lin2891 protein; PDBTitle: crystal structure of putative pyridoxamine 5'-phosphate oxidase2 (np_472219.1) from listeria innocua at 2.00 a resolution 31 c2ywjA_ not modelled 5.8 9 PDB header:transferase Chain: A: PDB Molecule:glutamine amidotransferase subunit pdxt; PDBTitle: crystal structure of uncharacterized conserved protein from2 methanocaldococcus jannaschii 32 c2yueA_ not modelled 5.6 16 PDB header:rna binding protein Chain: A: PDB Molecule:protein neuralized; PDBTitle: solution structure of the neuz (nhr) domain in neuralized2 from drosophila melanogaster 33 c3k6jA_ not modelled 5.6 21 PDB header:oxidoreductase Chain: A: PDB Molecule:protein f01g10.3, confirmed by transcript evidence; PDBTitle: crystal structure of the dehydrogenase part of multifuctional enzyme 12 from c.elegans 34 d1gq2a2 not modelled 5.1 7 Fold:Aminoacid dehydrogenase-like, N-terminal domain Superfamily:Aminoacid dehydrogenase-like, N-terminal domain Family:Malic enzyme N-domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0