BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (46 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q2EET0 Putative uncharacterized protein ypdJ n=9 Tax=Es...   100   2e-20
UniRef50_O22010 Excisionase n=13 Tax=root RepID=VXIS_BPSF5             94   1e-18

>UniRef50_Q2EET0 Putative uncharacterized protein ypdJ n=9 Tax=Escherichia coli
          RepID=YPDJ_ECOLI
          Length = 46

 Score =  100 bits (248), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46
          MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS
Sbjct: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46


>UniRef50_O22010 Excisionase n=13 Tax=root RepID=VXIS_BPSF5
          Length = 147

 Score = 94.0 bits (232), Expect = 1e-18,   Method: Compositional matrix adjust.
 Identities = 42/46 (91%), Positives = 43/46 (93%)

Query: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46
          MGYDSRLD LAATSWYPFFNNVT RGEI+EPYSLTLDEAC FLKIS
Sbjct: 4  MGYDSRLDRLAATSWYPFFNNVTARGEIMEPYSLTLDEACDFLKIS 49


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_O22010 Excisionase n=13 Tax=root RepID=VXIS_BPSF5            100   2e-20
UniRef50_Q2EET0 Putative uncharacterized protein ypdJ n=9 Tax=Es...    93   2e-18

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_O22010 Excisionase n=13 Tax=root RepID=VXIS_BPSF5
          Length = 147

 Score = 99.6 bits (247), Expect = 2e-20,   Method: Composition-based stats.
 Identities = 42/46 (91%), Positives = 43/46 (93%)

Query: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46
          MGYDSRLD LAATSWYPFFNNVT RGEI+EPYSLTLDEAC FLKIS
Sbjct: 4  MGYDSRLDRLAATSWYPFFNNVTARGEIMEPYSLTLDEACDFLKIS 49


>UniRef50_Q2EET0 Putative uncharacterized protein ypdJ n=9 Tax=Escherichia coli
          RepID=YPDJ_ECOLI
          Length = 46

 Score = 93.5 bits (231), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46
          MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS
Sbjct: 1  MGYDSRLDHLAATSWYPFFNNVTTRGEIIEPYSLTLDEACQFLKIS 46


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.318    0.144    0.464 

Lambda     K      H
   0.267   0.0441    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 201,047,995
Number of Sequences: 3077464
Number of extensions: 4924160
Number of successful extensions: 10309
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 10305
Number of HSP's gapped (non-prelim): 4
length of query: 46
length of database: 1,040,396,356
effective HSP length: 20
effective length of query: 26
effective length of database: 978,847,076
effective search space: 25450023976
effective search space used: 25450023976
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 87 (38.0 bits)