BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (271 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P77393 Uncharacterized protein yahL n=37 Tax=Enterobact...   560   e-158
UniRef50_B1EKY1 Putative uncharacterized protein n=1 Tax=Escheri...    46   0.001

>UniRef50_P77393 Uncharacterized protein yahL n=37 Tax=Enterobacteriaceae
           RepID=YAHL_ECOLI
          Length = 271

 Score =  560 bits (1444), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 271/271 (100%), Positives = 271/271 (100%)

Query: 1   MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD 60
           MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD
Sbjct: 1   MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD 60

Query: 61  LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG 120
           LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG
Sbjct: 61  LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG 120

Query: 121 TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE 180
           TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE
Sbjct: 121 TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE 180

Query: 181 CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD 240
           CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD
Sbjct: 181 CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD 240

Query: 241 ARLNEWISNRVQELGDRYYPVNKHVKIRYTL 271
           ARLNEWISNRVQELGDRYYPVNKHVKIRYTL
Sbjct: 241 ARLNEWISNRVQELGDRYYPVNKHVKIRYTL 271


>UniRef50_B1EKY1 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1EKY1_9ESCH
          Length = 159

 Score = 45.8 bits (107), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 38/153 (24%), Positives = 66/153 (43%), Gaps = 17/153 (11%)

Query: 21  NTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWS 80
           N V  + L     S L +C + F +++    +Y Y     L++D   I+L+ ++   ++S
Sbjct: 11  NEVNKHGLSIKSSSVLRACANYFAIMLNGRSEYNYSHIKKLLNDVNFINLITSIITSQFS 70

Query: 81  --DDNALTINVQFND-----FPGFYDWMDYPDHPVKFVFHILENQKGTVWVYDQDDAFLD 133
             D+N L I+V+F       FP      D PD+ V F   +     G +WV + ++  L 
Sbjct: 71  PRDNNVLAIHVKFTSEFRKSFP------DLPDYEVIFKIKVSPGGGGKIWVENINERKLC 124

Query: 134 IKANVQAGRFTGLKKLVQFIDSVRTDCKCILLE 166
               +       LK +  FI    +  KC  +E
Sbjct: 125 GDYKLYGKTLEALKNMDDFI----SQKKCFFIE 153


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P77393 Uncharacterized protein yahL n=37 Tax=Enterobact...   496   e-139
UniRef50_B1EKY1 Putative uncharacterized protein n=1 Tax=Escheri...   201   2e-50

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P77393 Uncharacterized protein yahL n=37 Tax=Enterobacteriaceae
           RepID=YAHL_ECOLI
          Length = 271

 Score =  496 bits (1277), Expect = e-139,   Method: Composition-based stats.
 Identities = 271/271 (100%), Positives = 271/271 (100%)

Query: 1   MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD 60
           MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD
Sbjct: 1   MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDD 60

Query: 61  LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG 120
           LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG
Sbjct: 61  LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKG 120

Query: 121 TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE 180
           TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE
Sbjct: 121 TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEYHMPLLRIFPKGKE 180

Query: 181 CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD 240
           CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD
Sbjct: 181 CMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDD 240

Query: 241 ARLNEWISNRVQELGDRYYPVNKHVKIRYTL 271
           ARLNEWISNRVQELGDRYYPVNKHVKIRYTL
Sbjct: 241 ARLNEWISNRVQELGDRYYPVNKHVKIRYTL 271


>UniRef50_B1EKY1 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1EKY1_9ESCH
          Length = 159

 Score =  201 bits (512), Expect = 2e-50,   Method: Composition-based stats.
 Identities = 36/148 (24%), Positives = 65/148 (43%), Gaps = 7/148 (4%)

Query: 21  NTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWS 80
           N V  + L     S L +C + F +++    +Y Y     L++D   I+L+ ++   ++S
Sbjct: 11  NEVNKHGLSIKSSSVLRACANYFAIMLNGRSEYNYSHIKKLLNDVNFINLITSIITSQFS 70

Query: 81  --DDNALTINVQFNDFPGFYDWMDYPDHPVKFVFHILENQKGTVWVYDQDDAFLDIKANV 138
             D+N L I+V+F        + D PD+ V F   +     G +WV + ++  L     +
Sbjct: 71  PRDNNVLAIHVKFTS-EFRKSFPDLPDYEVIFKIKVSPGGGGKIWVENINERKLCGDYKL 129

Query: 139 QAGRFTGLKKLVQFIDSVRTDCKCILLE 166
                  LK +  FI    +  KC  +E
Sbjct: 130 YGKTLEALKNMDDFI----SQKKCFFIE 153


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.322    0.137    0.399 

Lambda     K      H
   0.267   0.0421    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,119,857,033
Number of Sequences: 3077464
Number of extensions: 45458766
Number of successful extensions: 108174
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 108166
Number of HSP's gapped (non-prelim): 7
length of query: 271
length of database: 1,040,396,356
effective HSP length: 127
effective length of query: 144
effective length of database: 649,558,428
effective search space: 93536413632
effective search space used: 93536413632
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 92 (40.0 bits)