Processing 1 helix: Transmembrane helix 1 from 250 (in) to 265 (out) : 410.289 Score = 0.402411 Processing 1 helix: Transmembrane helix 1 from 250 (out) to 265 (in) : 409.511 Score = 0.417389 Processing 16 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -941.105 Transmembrane helix 2 from 32 (out) to 47 (in) : -76.478 Transmembrane helix 3 from 51 (in) to 66 (out) : -71.333 Transmembrane helix 4 from 70 (out) to 85 (in) : -99.701 Transmembrane helix 5 from 89 (in) to 104 (out) : -232.52 Transmembrane helix 6 from 108 (out) to 123 (in) : -465.296 Transmembrane helix 7 from 127 (in) to 142 (out) : 58.691 Transmembrane helix 8 from 146 (out) to 161 (in) : -521.044 Transmembrane helix 9 from 165 (in) to 180 (out) : -364.268 Transmembrane helix 10 from 184 (out) to 199 (in) : -306.965 Transmembrane helix 11 from 203 (in) to 218 (out) : -482.406 Transmembrane helix 12 from 222 (out) to 237 (in) : -735.977 Transmembrane helix 13 from 241 (in) to 256 (out) : 103.262 Transmembrane helix 14 from 260 (out) to 275 (in) : 59.915 Transmembrane helix 15 from 279 (in) to 294 (out) : -456.962 Transmembrane helix 16 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 23 (out) to 38 (in) : -275.555 Transmembrane helix 2 from 42 (in) to 57 (out) : -89.135 Transmembrane helix 3 from 61 (out) to 76 (in) : -72.951 Transmembrane helix 4 from 80 (in) to 95 (out) : -143.26 Transmembrane helix 5 from 99 (out) to 114 (in) : -535.166 Transmembrane helix 6 from 118 (in) to 133 (out) : 112.199 Transmembrane helix 7 from 137 (out) to 152 (in) : -323.255 Transmembrane helix 8 from 156 (in) to 171 (out) : -388.431 Transmembrane helix 9 from 175 (out) to 190 (in) : -339.275 Transmembrane helix 10 from 194 (in) to 209 (out) : -419.368 Transmembrane helix 11 from 213 (out) to 228 (in) : -556.719 Transmembrane helix 12 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 13 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 14 from 278 (in) to 293 (out) : -457.045 Transmembrane helix 15 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 118 (in) to 133 (out) : 112.199 Transmembrane helix 6 from 137 (out) to 152 (in) : -323.255 Transmembrane helix 7 from 156 (in) to 171 (out) : -388.431 Transmembrane helix 8 from 175 (out) to 190 (in) : -339.275 Transmembrane helix 9 from 194 (in) to 209 (out) : -419.368 Transmembrane helix 10 from 213 (out) to 228 (in) : -556.719 Transmembrane helix 11 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 12 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 13 from 278 (in) to 293 (out) : -457.045 Transmembrane helix 14 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 119 (out) to 134 (in) : 133.097 Transmembrane helix 6 from 138 (in) to 153 (out) : -374.649 Transmembrane helix 7 from 158 (out) to 173 (in) : -358.232 Transmembrane helix 8 from 180 (in) to 195 (out) : -295.081 Transmembrane helix 9 from 199 (out) to 214 (in) : -477.989 Transmembrane helix 10 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 11 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 12 from 278 (in) to 293 (out) : -457.045 Transmembrane helix 13 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 119 (in) to 134 (out) : 141.103 Transmembrane helix 6 from 138 (out) to 153 (in) : -351.151 Transmembrane helix 7 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 8 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 9 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 10 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 11 from 278 (in) to 293 (out) : -457.045 Transmembrane helix 12 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 6 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 7 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 8 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 9 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 10 from 278 (in) to 293 (out) : -457.045 Transmembrane helix 11 from 298 (out) to 313 (in) : -426.221 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 119 (in) to 134 (out) : 141.103 Transmembrane helix 6 from 138 (out) to 153 (in) : -351.151 Transmembrane helix 7 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 8 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 9 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 10 from 259 (out) to 274 (in) : 123.462 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 6 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 7 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 8 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 9 from 259 (out) to 274 (in) : 123.462 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 6 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 7 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 8 from 259 (out) to 274 (in) : 123.462 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 6 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 7 from 259 (out) to 274 (in) : 123.462 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 6 from 250 (out) to 265 (in) : 409.511 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 31 (out) to 46 (in) : -78.587 Transmembrane helix 2 from 50 (in) to 65 (out) : -71.678 Transmembrane helix 3 from 69 (out) to 84 (in) : -95.922 Transmembrane helix 4 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 5 from 250 (out) to 265 (in) : 409.511 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -57.915 Transmembrane helix 2 from 57 (out) to 72 (in) : -71.691 Transmembrane helix 3 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 4 from 250 (out) to 265 (in) : 409.511 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 62 (out) to 77 (in) : -76.079 Transmembrane helix 2 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 3 from 250 (out) to 265 (in) : 409.511 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 2 from 250 (out) to 265 (in) : 409.511 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -949.795 Transmembrane helix 2 from 32 (in) to 47 (out) : -69.322 Transmembrane helix 3 from 51 (out) to 66 (in) : -83.567 Transmembrane helix 4 from 70 (in) to 85 (out) : -98.899 Transmembrane helix 5 from 89 (out) to 104 (in) : -226.18 Transmembrane helix 6 from 108 (in) to 123 (out) : -460.304 Transmembrane helix 7 from 127 (out) to 142 (in) : 47.509 Transmembrane helix 8 from 146 (in) to 161 (out) : -520.256 Transmembrane helix 9 from 165 (out) to 180 (in) : -389.932 Transmembrane helix 10 from 184 (in) to 199 (out) : -313.235 Transmembrane helix 11 from 203 (out) to 218 (in) : -481.994 Transmembrane helix 12 from 222 (in) to 237 (out) : -743.723 Transmembrane helix 13 from 241 (out) to 256 (in) : 98.438 Transmembrane helix 14 from 260 (in) to 275 (out) : 62.885 Transmembrane helix 15 from 279 (out) to 294 (in) : -456.038 Transmembrane helix 16 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -240.296 Transmembrane helix 2 from 43 (out) to 58 (in) : -87.292 Transmembrane helix 3 from 62 (in) to 77 (out) : -79.321 Transmembrane helix 4 from 81 (out) to 96 (in) : -152.104 Transmembrane helix 5 from 100 (in) to 115 (out) : -578.534 Transmembrane helix 6 from 119 (out) to 134 (in) : 133.097 Transmembrane helix 7 from 138 (in) to 153 (out) : -374.649 Transmembrane helix 8 from 157 (out) to 172 (in) : -362.796 Transmembrane helix 9 from 176 (in) to 191 (out) : -347.933 Transmembrane helix 10 from 195 (out) to 210 (in) : -458.524 Transmembrane helix 11 from 214 (in) to 229 (out) : -566.235 Transmembrane helix 12 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 13 from 259 (in) to 274 (out) : 125.038 Transmembrane helix 14 from 279 (out) to 294 (in) : -456.038 Transmembrane helix 15 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.99 Transmembrane helix 2 from 44 (in) to 59 (out) : -89.186 Transmembrane helix 3 from 63 (out) to 78 (in) : -78.243 Transmembrane helix 4 from 82 (in) to 97 (out) : -155.026 Transmembrane helix 5 from 101 (out) to 116 (in) : -592.962 Transmembrane helix 6 from 120 (in) to 135 (out) : 159.067 Transmembrane helix 7 from 139 (out) to 154 (in) : -378.333 Transmembrane helix 8 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 9 from 179 (out) to 194 (in) : -277.019 Transmembrane helix 10 from 198 (in) to 213 (out) : -466.31 Transmembrane helix 11 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 12 from 259 (in) to 274 (out) : 125.038 Transmembrane helix 13 from 279 (out) to 294 (in) : -456.038 Transmembrane helix 14 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 119 (in) to 134 (out) : 141.103 Transmembrane helix 6 from 138 (out) to 153 (in) : -351.151 Transmembrane helix 7 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 8 from 179 (out) to 194 (in) : -277.019 Transmembrane helix 9 from 198 (in) to 213 (out) : -466.31 Transmembrane helix 10 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 11 from 259 (in) to 274 (out) : 125.038 Transmembrane helix 12 from 279 (out) to 294 (in) : -456.038 Transmembrane helix 13 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 119 (out) to 134 (in) : 133.097 Transmembrane helix 6 from 138 (in) to 153 (out) : -374.649 Transmembrane helix 7 from 158 (out) to 173 (in) : -358.232 Transmembrane helix 8 from 180 (in) to 195 (out) : -295.081 Transmembrane helix 9 from 199 (out) to 214 (in) : -477.989 Transmembrane helix 10 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 11 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 12 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 119 (in) to 134 (out) : 141.103 Transmembrane helix 6 from 138 (out) to 153 (in) : -351.151 Transmembrane helix 7 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 8 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 9 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 10 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 11 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 6 from 160 (in) to 175 (out) : -367.403 Transmembrane helix 7 from 180 (out) to 195 (in) : -271.719 Transmembrane helix 8 from 240 (in) to 255 (out) : 61.512 Transmembrane helix 9 from 259 (out) to 274 (in) : 123.462 Transmembrane helix 10 from 298 (in) to 313 (out) : -423.479 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 119 (in) to 134 (out) : 141.103 Transmembrane helix 6 from 138 (out) to 153 (in) : -351.151 Transmembrane helix 7 from 181 (in) to 196 (out) : -295.01 Transmembrane helix 8 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 9 from 259 (in) to 274 (out) : 125.038 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -111.121 Transmembrane helix 2 from 47 (in) to 62 (out) : -78.166 Transmembrane helix 3 from 66 (out) to 81 (in) : -85.826 Transmembrane helix 4 from 85 (in) to 100 (out) : -174.892 Transmembrane helix 5 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 6 from 181 (in) to 196 (out) : -295.01 Transmembrane helix 7 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 8 from 259 (in) to 274 (out) : 125.038 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 28 (in) to 43 (out) : -118.279 Transmembrane helix 2 from 47 (out) to 62 (in) : -88.834 Transmembrane helix 3 from 66 (in) to 81 (out) : -89.074 Transmembrane helix 4 from 85 (out) to 100 (in) : -172.008 Transmembrane helix 5 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 6 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 7 from 259 (in) to 274 (out) : 125.038 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 31 (out) to 46 (in) : -78.587 Transmembrane helix 2 from 50 (in) to 65 (out) : -71.678 Transmembrane helix 3 from 69 (out) to 84 (in) : -95.922 Transmembrane helix 4 from 121 (in) to 136 (out) : 166.708 Transmembrane helix 5 from 240 (out) to 255 (in) : 55.888 Transmembrane helix 6 from 259 (in) to 274 (out) : 125.038 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -57.915 Transmembrane helix 2 from 54 (out) to 69 (in) : -77.51 Transmembrane helix 3 from 73 (in) to 88 (out) : -107.971 Transmembrane helix 4 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 5 from 250 (in) to 265 (out) : 410.289 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -63.485 Transmembrane helix 2 from 59 (in) to 74 (out) : -74.465 Transmembrane helix 3 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 4 from 250 (in) to 265 (out) : 410.289 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -57.915 Transmembrane helix 2 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 3 from 250 (in) to 265 (out) : 410.289 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 121 (out) to 136 (in) : 155.692 Transmembrane helix 2 from 250 (in) to 265 (out) : 410.289 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.402411 1 helix (-) : Score = 0.417389 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 250-265 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.417389