Processing 1 helix: Transmembrane helix 1 from 50 (in) to 80 (out) : 4506.07 Score = 4.515709 Processing 1 helix: Transmembrane helix 1 from 50 (out) to 80 (in) : 4517.13 Score = 4.507491 Processing 19 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -9423.35 Transmembrane helix 2 from 29 (out) to 44 (in) : -640.695 Transmembrane helix 3 from 48 (in) to 63 (out) : 2406.07 Transmembrane helix 4 from 67 (out) to 82 (in) : 2938.77 Transmembrane helix 5 from 86 (in) to 101 (out) : 1.331 Transmembrane helix 6 from 105 (out) to 120 (in) : -1.61 Transmembrane helix 7 from 124 (in) to 139 (out) : 2.418 Transmembrane helix 8 from 143 (out) to 158 (in) : -7.534 Transmembrane helix 9 from 162 (in) to 177 (out) : -3.329 Transmembrane helix 10 from 181 (out) to 196 (in) : 11.518 Transmembrane helix 11 from 200 (in) to 215 (out) : 7.174 Transmembrane helix 12 from 219 (out) to 234 (in) : 4.651 Transmembrane helix 13 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 14 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 15 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 16 from 295 (out) to 310 (in) : 0 Transmembrane helix 17 from 314 (in) to 329 (out) : 0 Transmembrane helix 18 from 333 (out) to 348 (in) : 0 Transmembrane helix 19 from 352 (in) to 367 (out) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 29 (out) to 44 (in) : -640.695 Transmembrane helix 2 from 48 (in) to 63 (out) : 2406.07 Transmembrane helix 3 from 67 (out) to 82 (in) : 2938.77 Transmembrane helix 4 from 86 (in) to 101 (out) : 1.331 Transmembrane helix 5 from 105 (out) to 120 (in) : -1.61 Transmembrane helix 6 from 124 (in) to 139 (out) : 2.418 Transmembrane helix 7 from 143 (out) to 158 (in) : -7.534 Transmembrane helix 8 from 162 (in) to 177 (out) : -3.329 Transmembrane helix 9 from 181 (out) to 196 (in) : 11.518 Transmembrane helix 10 from 200 (in) to 215 (out) : 7.174 Transmembrane helix 11 from 219 (out) to 234 (in) : 4.651 Transmembrane helix 12 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 13 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 14 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 15 from 295 (out) to 310 (in) : 0 Transmembrane helix 16 from 314 (in) to 329 (out) : 0 Transmembrane helix 17 from 333 (out) to 348 (in) : 0 Transmembrane helix 18 from 352 (in) to 367 (out) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 67 (out) to 82 (in) : 2938.77 Transmembrane helix 3 from 86 (in) to 101 (out) : 1.331 Transmembrane helix 4 from 105 (out) to 120 (in) : -1.61 Transmembrane helix 5 from 124 (in) to 139 (out) : 2.418 Transmembrane helix 6 from 143 (out) to 158 (in) : -7.534 Transmembrane helix 7 from 162 (in) to 177 (out) : -3.329 Transmembrane helix 8 from 181 (out) to 196 (in) : 11.518 Transmembrane helix 9 from 200 (in) to 215 (out) : 7.174 Transmembrane helix 10 from 219 (out) to 234 (in) : 4.651 Transmembrane helix 11 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 12 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 13 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 14 from 295 (out) to 310 (in) : 0 Transmembrane helix 15 from 314 (in) to 329 (out) : 0 Transmembrane helix 16 from 333 (out) to 348 (in) : 0 Transmembrane helix 17 from 352 (in) to 367 (out) : 0 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 83 (out) : 2933.72 Transmembrane helix 3 from 87 (out) to 102 (in) : -2.753 Transmembrane helix 4 from 106 (in) to 121 (out) : 0.801 Transmembrane helix 5 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 6 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 7 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 8 from 200 (in) to 215 (out) : 7.174 Transmembrane helix 9 from 219 (out) to 234 (in) : 4.651 Transmembrane helix 10 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 11 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 12 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 13 from 295 (out) to 310 (in) : 0 Transmembrane helix 14 from 314 (in) to 329 (out) : 0 Transmembrane helix 15 from 333 (out) to 348 (in) : 0 Transmembrane helix 16 from 352 (in) to 367 (out) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 105 (out) : 7.022 Transmembrane helix 4 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 5 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 6 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 7 from 200 (in) to 215 (out) : 7.174 Transmembrane helix 8 from 219 (out) to 234 (in) : 4.651 Transmembrane helix 9 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 10 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 11 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 12 from 295 (out) to 310 (in) : 0 Transmembrane helix 13 from 314 (in) to 329 (out) : 0 Transmembrane helix 14 from 333 (out) to 348 (in) : 0 Transmembrane helix 15 from 352 (in) to 367 (out) : 0 Re-entrant helix 1 from 109 (out) to 122 (out) : 980 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 83 (out) : 2933.72 Transmembrane helix 3 from 87 (out) to 102 (in) : -2.753 Transmembrane helix 4 from 106 (in) to 121 (out) : 0.801 Transmembrane helix 5 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 6 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 7 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 8 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 9 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 10 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 11 from 295 (out) to 310 (in) : 0 Transmembrane helix 12 from 314 (in) to 329 (out) : 0 Transmembrane helix 13 from 333 (out) to 348 (in) : 0 Transmembrane helix 14 from 352 (in) to 367 (out) : 0 Re-entrant helix 1 from 194 (in) to 228 (in) : 2450 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 105 (out) : 7.022 Transmembrane helix 4 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 5 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 6 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 7 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 8 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 9 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 10 from 295 (out) to 310 (in) : 0 Transmembrane helix 11 from 314 (in) to 329 (out) : 0 Transmembrane helix 12 from 333 (out) to 348 (in) : 0 Transmembrane helix 13 from 352 (in) to 367 (out) : 0 Re-entrant helix 1 from 109 (out) to 122 (out) : 980 Re-entrant helix 2 from 194 (in) to 228 (in) : 2450 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 4 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 5 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 6 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 7 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 8 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 9 from 295 (out) to 310 (in) : 0 Transmembrane helix 10 from 314 (in) to 329 (out) : 0 Transmembrane helix 11 from 333 (out) to 348 (in) : 0 Transmembrane helix 12 from 352 (in) to 367 (out) : 0 Re-entrant helix 1 from 102 (out) to 122 (out) : 1470 Re-entrant helix 2 from 194 (in) to 228 (in) : 2450 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 238 (in) to 253 (out) : 9.143 Transmembrane helix 6 from 257 (out) to 272 (in) : 0.777 Transmembrane helix 7 from 276 (in) to 291 (out) : 3.178 Transmembrane helix 8 from 295 (out) to 310 (in) : 0 Transmembrane helix 9 from 314 (in) to 329 (out) : 0 Transmembrane helix 10 from 333 (out) to 348 (in) : 0 Transmembrane helix 11 from 352 (in) to 367 (out) : 0 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 4 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 5 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 6 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 7 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 8 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 9 from 302 (out) to 317 (in) : 0 Transmembrane helix 10 from 321 (in) to 336 (out) : 0 Re-entrant helix 1 from 102 (out) to 122 (out) : 1470 Re-entrant helix 2 from 194 (in) to 228 (in) : 2450 Re-entrant helix 3 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 6 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 7 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 8 from 302 (out) to 317 (in) : 0 Transmembrane helix 9 from 321 (in) to 336 (out) : 0 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 5 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 6 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 7 from 302 (out) to 317 (in) : 0 Transmembrane helix 8 from 321 (in) to 336 (out) : 0 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 238 (in) : 770 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 6 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 7 from 283 (in) to 298 (out) : 3.637 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 5 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 6 from 283 (in) to 298 (out) : 3.637 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 238 (in) : 770 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 243 (in) to 258 (out) : 7.092 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 239 (in) : 770 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 243 (in) to 258 (out) : 7.092 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 239 (in) : 840 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 96 (in) : 2959.7 Transmembrane helix 3 from 196 (in) to 226 (out) : 18.547 Re-entrant helix 1 from 100 (in) to 134 (in) : 2450 Re-entrant helix 2 from 135 (in) to 169 (in) : 2450 Re-entrant helix 3 from 170 (in) to 192 (in) : 1610 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 83 (out) : 2933.72 Score = 5.737137 Processing 19 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -9416.45 Transmembrane helix 2 from 29 (in) to 44 (out) : -649.105 Transmembrane helix 3 from 48 (out) to 63 (in) : 2375.13 Transmembrane helix 4 from 67 (in) to 82 (out) : 2914.53 Transmembrane helix 5 from 86 (out) to 101 (in) : -1.331 Transmembrane helix 6 from 105 (in) to 120 (out) : 1.61 Transmembrane helix 7 from 124 (out) to 139 (in) : -2.418 Transmembrane helix 8 from 143 (in) to 158 (out) : 7.534 Transmembrane helix 9 from 162 (out) to 177 (in) : 3.329 Transmembrane helix 10 from 181 (in) to 196 (out) : -11.518 Transmembrane helix 11 from 200 (out) to 215 (in) : -7.174 Transmembrane helix 12 from 219 (in) to 234 (out) : -4.651 Transmembrane helix 13 from 238 (out) to 253 (in) : -9.143 Transmembrane helix 14 from 257 (in) to 272 (out) : -0.777 Transmembrane helix 15 from 276 (out) to 291 (in) : -3.178 Transmembrane helix 16 from 295 (in) to 310 (out) : 0 Transmembrane helix 17 from 314 (out) to 329 (in) : 0 Transmembrane helix 18 from 333 (in) to 348 (out) : 0 Transmembrane helix 19 from 352 (out) to 367 (in) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 29 (in) to 44 (out) : -649.105 Transmembrane helix 2 from 48 (out) to 63 (in) : 2375.13 Transmembrane helix 3 from 67 (in) to 82 (out) : 2914.53 Transmembrane helix 4 from 86 (out) to 101 (in) : -1.331 Transmembrane helix 5 from 105 (in) to 120 (out) : 1.61 Transmembrane helix 6 from 124 (out) to 139 (in) : -2.418 Transmembrane helix 7 from 143 (in) to 158 (out) : 7.534 Transmembrane helix 8 from 162 (out) to 177 (in) : 3.329 Transmembrane helix 9 from 181 (in) to 196 (out) : -11.518 Transmembrane helix 10 from 200 (out) to 215 (in) : -7.174 Transmembrane helix 11 from 219 (in) to 234 (out) : -4.651 Transmembrane helix 12 from 238 (out) to 253 (in) : -9.143 Transmembrane helix 13 from 257 (in) to 272 (out) : -0.777 Transmembrane helix 14 from 276 (out) to 291 (in) : -3.178 Transmembrane helix 15 from 295 (in) to 310 (out) : 0 Transmembrane helix 16 from 314 (out) to 329 (in) : 0 Transmembrane helix 17 from 333 (in) to 348 (out) : 0 Transmembrane helix 18 from 352 (out) to 367 (in) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 67 (in) to 82 (out) : 2914.53 Transmembrane helix 3 from 86 (out) to 101 (in) : -1.331 Transmembrane helix 4 from 105 (in) to 120 (out) : 1.61 Transmembrane helix 5 from 124 (out) to 139 (in) : -2.418 Transmembrane helix 6 from 143 (in) to 158 (out) : 7.534 Transmembrane helix 7 from 162 (out) to 177 (in) : 3.329 Transmembrane helix 8 from 181 (in) to 196 (out) : -11.518 Transmembrane helix 9 from 200 (out) to 215 (in) : -7.174 Transmembrane helix 10 from 219 (in) to 234 (out) : -4.651 Transmembrane helix 11 from 238 (out) to 253 (in) : -9.143 Transmembrane helix 12 from 257 (in) to 272 (out) : -0.777 Transmembrane helix 13 from 276 (out) to 291 (in) : -3.178 Transmembrane helix 14 from 295 (in) to 310 (out) : 0 Transmembrane helix 15 from 314 (out) to 329 (in) : 0 Transmembrane helix 16 from 333 (in) to 348 (out) : 0 Transmembrane helix 17 from 352 (out) to 367 (in) : 0 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 83 (in) : 2955.68 Transmembrane helix 3 from 87 (in) to 102 (out) : 2.753 Transmembrane helix 4 from 106 (out) to 121 (in) : -0.801 Transmembrane helix 5 from 125 (in) to 140 (out) : 1.244 Transmembrane helix 6 from 144 (out) to 159 (in) : -9.118 Transmembrane helix 7 from 163 (in) to 178 (out) : -4.322 Transmembrane helix 8 from 182 (out) to 197 (in) : 11.145 Transmembrane helix 9 from 203 (in) to 218 (out) : 4.355 Transmembrane helix 10 from 222 (out) to 237 (in) : 3.697 Transmembrane helix 11 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 12 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 13 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 14 from 302 (out) to 317 (in) : 0 Transmembrane helix 15 from 321 (in) to 336 (out) : 0 Transmembrane helix 16 from 340 (out) to 355 (in) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 83 (out) : 2933.72 Transmembrane helix 3 from 87 (out) to 102 (in) : -2.753 Transmembrane helix 4 from 106 (in) to 121 (out) : 0.801 Transmembrane helix 5 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 6 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 7 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 8 from 203 (in) to 218 (out) : 4.355 Transmembrane helix 9 from 222 (out) to 237 (in) : 3.697 Transmembrane helix 10 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 11 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 12 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 13 from 302 (out) to 317 (in) : 0 Transmembrane helix 14 from 321 (in) to 336 (out) : 0 Transmembrane helix 15 from 340 (out) to 355 (in) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 105 (out) : 7.022 Transmembrane helix 4 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 5 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 6 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 7 from 203 (in) to 218 (out) : 4.355 Transmembrane helix 8 from 222 (out) to 237 (in) : 3.697 Transmembrane helix 9 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 10 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 11 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 12 from 302 (out) to 317 (in) : 0 Transmembrane helix 13 from 321 (in) to 336 (out) : 0 Transmembrane helix 14 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 109 (out) to 122 (out) : 980 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 83 (out) : 2933.72 Transmembrane helix 3 from 87 (out) to 102 (in) : -2.753 Transmembrane helix 4 from 106 (in) to 121 (out) : 0.801 Transmembrane helix 5 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 6 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 7 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 8 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 9 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 10 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 11 from 302 (out) to 317 (in) : 0 Transmembrane helix 12 from 321 (in) to 336 (out) : 0 Transmembrane helix 13 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 194 (in) to 228 (in) : 2450 Re-entrant helix 2 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 105 (out) : 7.022 Transmembrane helix 4 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 5 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 6 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 7 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 8 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 9 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 10 from 302 (out) to 317 (in) : 0 Transmembrane helix 11 from 321 (in) to 336 (out) : 0 Transmembrane helix 12 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 109 (out) to 122 (out) : 980 Re-entrant helix 2 from 194 (in) to 228 (in) : 2450 Re-entrant helix 3 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 126 (out) to 141 (in) : -0.102 Transmembrane helix 4 from 145 (in) to 160 (out) : 10.013 Transmembrane helix 5 from 175 (out) to 190 (in) : 16.739 Transmembrane helix 6 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 7 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 8 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 9 from 302 (out) to 317 (in) : 0 Transmembrane helix 10 from 321 (in) to 336 (out) : 0 Transmembrane helix 11 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 102 (out) to 122 (out) : 1470 Re-entrant helix 2 from 194 (in) to 228 (in) : 2450 Re-entrant helix 3 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 6 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 7 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 8 from 302 (out) to 317 (in) : 0 Transmembrane helix 9 from 321 (in) to 336 (out) : 0 Transmembrane helix 10 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 5 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 6 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 7 from 302 (out) to 317 (in) : 0 Transmembrane helix 8 from 321 (in) to 336 (out) : 0 Transmembrane helix 9 from 340 (out) to 355 (in) : 0 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 238 (in) : 770 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 6 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 7 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 8 from 302 (out) to 317 (in) : 0 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 238 (in) : 700 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 242 (in) to 257 (out) : 8.433 Transmembrane helix 5 from 261 (out) to 276 (in) : -1.205 Transmembrane helix 6 from 283 (in) to 298 (out) : 3.637 Transmembrane helix 7 from 302 (out) to 317 (in) : 0 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 238 (in) : 770 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 190 (in) : 20.676 Transmembrane helix 5 from 243 (in) to 258 (out) : 7.092 Transmembrane helix 6 from 262 (out) to 285 (in) : 2.87 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Re-entrant helix 3 from 194 (in) to 228 (in) : 2450 Re-entrant helix 4 from 229 (in) to 239 (in) : 770 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Transmembrane helix 4 from 243 (in) to 258 (out) : 7.092 Transmembrane helix 5 from 262 (out) to 285 (in) : 2.87 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Re-entrant helix 3 from 193 (in) to 227 (in) : 2450 Re-entrant helix 4 from 228 (in) to 239 (in) : 840 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 86 (in) : 2958.07 Transmembrane helix 3 from 90 (in) to 111 (out) : 13.775 Transmembrane helix 4 from 172 (out) to 201 (in) : 34.012 Re-entrant helix 1 from 115 (out) to 149 (out) : 2450 Re-entrant helix 2 from 150 (out) to 168 (out) : 1330 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 43 (out) to 61 (in) : 2823.39 Transmembrane helix 2 from 68 (in) to 98 (out) : 2930.03 Transmembrane helix 3 from 159 (out) to 189 (in) : 24.646 Re-entrant helix 1 from 102 (out) to 136 (out) : 2450 Re-entrant helix 2 from 137 (out) to 155 (out) : 1330 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 43 (in) to 61 (out) : 2873.21 Transmembrane helix 2 from 68 (out) to 96 (in) : 2959.7 Score = 5.852883 Summary of topology analysis: 1 helix (+) : Score = 4.51571 1 helix (-) : Score = 4.50749 2 helices (+) : Score = 5.85288 2 helices (-) : Score = 5.73714 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 Results: Signal peptide: 1-20 Signal score: 27.346 Topology: 43-61,68-96 Re-entrant helices: Not detected. Helix count: 2 N-terminal: out Score: 5.85288