BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (77 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P76073 Uncharacterized protein ynaE n=32 Tax=Enterobact...   156   2e-37
UniRef50_B5Y2P1 Putative uncharacterized protein n=2 Tax=Enterob...   100   2e-20

>UniRef50_P76073 Uncharacterized protein ynaE n=32 Tax=Enterobacteriaceae
          RepID=YNAE_ECOLI
          Length = 88

 Score =  156 bits (395), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 77/77 (100%), Positives = 77/77 (100%)

Query: 1  MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 60
          MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL
Sbjct: 12 MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 71

Query: 61 QTAISVLKDNQNLNGKM 77
          QTAISVLKDNQNLNGKM
Sbjct: 72 QTAISVLKDNQNLNGKM 88


>UniRef50_B5Y2P1 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=B5Y2P1_KLEP3
          Length = 95

 Score =  100 bits (249), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 48/76 (63%), Positives = 61/76 (80%)

Query: 1  MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 60
          MKSKDTL W+PAQLP V+IILG+AV+ V KQGRPINTRTLL+Y++    K    D+K  +
Sbjct: 19 MKSKDTLDWYPAQLPPVKIILGEAVLAVGKQGRPINTRTLLEYLQVMQDKQKRRDDKIAM 78

Query: 61 QTAISVLKDNQNLNGK 76
          QTAI+VL+DNQ +NG+
Sbjct: 79 QTAINVLRDNQRINGR 94


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76073 Uncharacterized protein ynaE n=32 Tax=Enterobact...   127   1e-28
UniRef50_B5Y2P1 Putative uncharacterized protein n=2 Tax=Enterob...   125   6e-28

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P76073 Uncharacterized protein ynaE n=32 Tax=Enterobacteriaceae
          RepID=YNAE_ECOLI
          Length = 88

 Score =  127 bits (320), Expect = 1e-28,   Method: Composition-based stats.
 Identities = 77/77 (100%), Positives = 77/77 (100%)

Query: 1  MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 60
          MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL
Sbjct: 12 MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 71

Query: 61 QTAISVLKDNQNLNGKM 77
          QTAISVLKDNQNLNGKM
Sbjct: 72 QTAISVLKDNQNLNGKM 88


>UniRef50_B5Y2P1 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=B5Y2P1_KLEP3
          Length = 95

 Score =  125 bits (313), Expect = 6e-28,   Method: Composition-based stats.
 Identities = 48/76 (63%), Positives = 61/76 (80%)

Query: 1  MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYIEGNIKKTSWLDNKELL 60
          MKSKDTL W+PAQLP V+IILG+AV+ V KQGRPINTRTLL+Y++    K    D+K  +
Sbjct: 19 MKSKDTLDWYPAQLPPVKIILGEAVLAVGKQGRPINTRTLLEYLQVMQDKQKRRDDKIAM 78

Query: 61 QTAISVLKDNQNLNGK 76
          QTAI+VL+DNQ +NG+
Sbjct: 79 QTAINVLRDNQRINGR 94


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.138    0.394 

Lambda     K      H
   0.267   0.0396    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 279,889,143
Number of Sequences: 3077464
Number of extensions: 8437835
Number of successful extensions: 18066
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18062
Number of HSP's gapped (non-prelim): 4
length of query: 77
length of database: 1,040,396,356
effective HSP length: 48
effective length of query: 29
effective length of database: 892,678,084
effective search space: 25887664436
effective search space used: 25887664436
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 87 (38.2 bits)