Processing 1 helix: Transmembrane helix 1 from 50 (in) to 80 (out) : 3594.09 Score = 3.600747 Processing 1 helix: Transmembrane helix 1 from 50 (out) to 80 (in) : 3588.3 Score = 3.581652 Processing 12 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -468.335 Transmembrane helix 2 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 3 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 4 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 5 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 6 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 7 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 8 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 9 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 10 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 11 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 12 from 1 (out) to 0 (in) : -0.955 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -127.335 Transmembrane helix 2 from 23 (in) to 38 (out) : 833.521 Transmembrane helix 3 from 42 (out) to 57 (in) : 2760.35 Transmembrane helix 4 from 61 (in) to 76 (out) : 1415.34 Transmembrane helix 5 from 80 (out) to 95 (in) : 1323.3 Transmembrane helix 6 from 99 (in) to 114 (out) : 1625.48 Transmembrane helix 7 from 118 (out) to 133 (in) : -12.157 Transmembrane helix 8 from 137 (in) to 152 (out) : 1988.84 Transmembrane helix 9 from 156 (out) to 171 (in) : -632.104 Transmembrane helix 10 from 175 (in) to 190 (out) : 61.647 Transmembrane helix 11 from 194 (out) to 209 (in) : 1242.41 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -122.365 Transmembrane helix 2 from 23 (out) to 38 (in) : 826.979 Transmembrane helix 3 from 42 (in) to 57 (out) : 2772.45 Transmembrane helix 4 from 61 (out) to 76 (in) : 1414.86 Transmembrane helix 5 from 80 (in) to 95 (out) : 1339.4 Transmembrane helix 6 from 99 (out) to 115 (in) : 1641.8 Transmembrane helix 7 from 127 (in) to 142 (out) : 1790.38 Transmembrane helix 8 from 146 (out) to 161 (in) : 425.575 Transmembrane helix 9 from 172 (in) to 187 (out) : -365.795 Transmembrane helix 10 from 191 (out) to 206 (in) : 1811.97 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -127.335 Transmembrane helix 2 from 23 (in) to 38 (out) : 833.521 Transmembrane helix 3 from 42 (out) to 57 (in) : 2760.35 Transmembrane helix 4 from 61 (in) to 76 (out) : 1415.34 Transmembrane helix 5 from 80 (out) to 95 (in) : 1323.3 Transmembrane helix 6 from 99 (in) to 115 (out) : 1648.6 Transmembrane helix 7 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 8 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 9 from 186 (out) to 204 (in) : 2133.71 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : 1116.2 Transmembrane helix 2 from 37 (out) to 56 (in) : 3061.43 Transmembrane helix 3 from 60 (in) to 76 (out) : 1444.28 Transmembrane helix 4 from 80 (out) to 95 (in) : 1323.3 Transmembrane helix 5 from 99 (in) to 115 (out) : 1648.6 Transmembrane helix 6 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 7 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 8 from 186 (out) to 204 (in) : 2133.71 Score = 13.057429 Processing 7 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : 1118 Transmembrane helix 2 from 37 (in) to 60 (out) : 3162.04 Transmembrane helix 3 from 68 (out) to 89 (in) : 3357.83 Transmembrane helix 4 from 98 (in) to 115 (out) : 1651.22 Transmembrane helix 5 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 6 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 7 from 186 (out) to 204 (in) : 2133.71 Score = 13.743238 Processing 6 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : 1116.2 Transmembrane helix 2 from 37 (out) to 60 (in) : 3151.96 Transmembrane helix 3 from 68 (in) to 89 (out) : 3371.37 Transmembrane helix 4 from 98 (out) to 115 (in) : 1645.97 Transmembrane helix 5 from 129 (in) to 155 (out) : 2695.53 Transmembrane helix 6 from 186 (out) to 204 (in) : 2133.71 Score = 14.196778 Processing 5 helices: Transmembrane helix 1 from 26 (out) to 56 (in) : 3505.72 Transmembrane helix 2 from 68 (in) to 89 (out) : 3371.37 Transmembrane helix 3 from 98 (out) to 115 (in) : 1645.97 Transmembrane helix 4 from 129 (in) to 155 (out) : 2695.53 Transmembrane helix 5 from 186 (out) to 204 (in) : 2133.71 Score = 13.432416 Processing 4 helices: Transmembrane helix 1 from 26 (in) to 56 (out) : 3506.58 Transmembrane helix 2 from 70 (out) to 100 (in) : 3368.94 Transmembrane helix 3 from 129 (in) to 155 (out) : 2695.53 Transmembrane helix 4 from 186 (out) to 204 (in) : 2133.71 Score = 11.780963 Processing 3 helices: Transmembrane helix 1 from 26 (out) to 56 (in) : 3505.72 Transmembrane helix 2 from 68 (in) to 89 (out) : 3371.37 Transmembrane helix 3 from 129 (out) to 155 (in) : 2701.66 Score = 9.590135 Processing 2 helices: Transmembrane helix 1 from 26 (in) to 56 (out) : 3506.58 Transmembrane helix 2 from 70 (out) to 100 (in) : 3368.94 Score = 6.878863 Processing 12 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -472.865 Transmembrane helix 2 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 3 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 4 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 5 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 6 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 7 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 8 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 9 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 10 from 1 (in) to 0 (out) : 0.955 Transmembrane helix 11 from 1 (out) to 0 (in) : -0.955 Transmembrane helix 12 from 1 (in) to 0 (out) : 0.955 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -122.365 Transmembrane helix 2 from 23 (out) to 38 (in) : 826.979 Transmembrane helix 3 from 42 (in) to 57 (out) : 2772.45 Transmembrane helix 4 from 61 (out) to 76 (in) : 1414.86 Transmembrane helix 5 from 80 (in) to 95 (out) : 1339.4 Transmembrane helix 6 from 99 (out) to 114 (in) : 1619.92 Transmembrane helix 7 from 118 (in) to 133 (out) : -18.443 Transmembrane helix 8 from 137 (out) to 152 (in) : 1991.76 Transmembrane helix 9 from 156 (in) to 171 (out) : -612.296 Transmembrane helix 10 from 175 (out) to 190 (in) : 81.453 Transmembrane helix 11 from 194 (in) to 209 (out) : 1239.39 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -127.335 Transmembrane helix 2 from 23 (in) to 38 (out) : 833.521 Transmembrane helix 3 from 42 (out) to 57 (in) : 2760.35 Transmembrane helix 4 from 61 (in) to 76 (out) : 1415.34 Transmembrane helix 5 from 80 (out) to 95 (in) : 1323.3 Transmembrane helix 6 from 99 (in) to 115 (out) : 1648.6 Transmembrane helix 7 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 8 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 9 from 173 (out) to 188 (in) : -201.512 Transmembrane helix 10 from 192 (in) to 207 (out) : 1658 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : 1116.2 Transmembrane helix 2 from 37 (out) to 56 (in) : 3061.43 Transmembrane helix 3 from 60 (in) to 76 (out) : 1444.28 Transmembrane helix 4 from 80 (out) to 95 (in) : 1323.3 Transmembrane helix 5 from 99 (in) to 115 (out) : 1648.6 Transmembrane helix 6 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 7 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 8 from 173 (out) to 188 (in) : -201.512 Transmembrane helix 9 from 192 (in) to 207 (out) : 1658 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : 1118 Transmembrane helix 2 from 37 (in) to 60 (out) : 3162.04 Transmembrane helix 3 from 68 (out) to 89 (in) : 3357.83 Transmembrane helix 4 from 98 (in) to 115 (out) : 1651.22 Transmembrane helix 5 from 127 (out) to 142 (in) : 1804.62 Transmembrane helix 6 from 146 (in) to 161 (out) : 446.025 Transmembrane helix 7 from 173 (out) to 188 (in) : -201.512 Transmembrane helix 8 from 192 (in) to 207 (out) : 1658 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : 1116.2 Transmembrane helix 2 from 37 (out) to 60 (in) : 3151.96 Transmembrane helix 3 from 68 (in) to 89 (out) : 3371.37 Transmembrane helix 4 from 98 (out) to 115 (in) : 1645.97 Transmembrane helix 5 from 127 (in) to 142 (out) : 1790.38 Transmembrane helix 6 from 146 (out) to 161 (in) : 425.575 Transmembrane helix 7 from 186 (in) to 204 (out) : 2092.89 Score = 13.524561 Processing 6 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : 1118 Transmembrane helix 2 from 37 (in) to 60 (out) : 3162.04 Transmembrane helix 3 from 68 (out) to 89 (in) : 3357.83 Transmembrane helix 4 from 98 (in) to 115 (out) : 1651.22 Transmembrane helix 5 from 129 (out) to 155 (in) : 2701.66 Transmembrane helix 6 from 186 (in) to 204 (out) : 2092.89 Score = 14.001619 Processing 5 helices: Transmembrane helix 1 from 26 (in) to 56 (out) : 3506.58 Transmembrane helix 2 from 68 (out) to 89 (in) : 3357.83 Transmembrane helix 3 from 98 (in) to 115 (out) : 1651.22 Transmembrane helix 4 from 129 (out) to 155 (in) : 2701.66 Transmembrane helix 5 from 186 (in) to 204 (out) : 2092.89 Score = 13.230082 Processing 4 helices: Transmembrane helix 1 from 26 (out) to 56 (in) : 3505.72 Transmembrane helix 2 from 68 (in) to 89 (out) : 3371.37 Transmembrane helix 3 from 129 (out) to 155 (in) : 2701.66 Transmembrane helix 4 from 186 (in) to 204 (out) : 2092.89 Score = 11.608609 Processing 3 helices: Transmembrane helix 1 from 26 (in) to 56 (out) : 3506.58 Transmembrane helix 2 from 70 (out) to 100 (in) : 3368.94 Transmembrane helix 3 from 129 (in) to 155 (out) : 2695.53 Score = 9.572838 Processing 2 helices: Transmembrane helix 1 from 26 (out) to 56 (in) : 3505.72 Transmembrane helix 2 from 70 (in) to 100 (out) : 3382.86 Score = 6.885235 Summary of topology analysis: 1 helix (+) : Score = 3.60075 1 helix (-) : Score = 3.58165 2 helices (+) : Score = 6.87886 2 helices (-) : Score = 6.88524 3 helices (+) : Score = 9.57284 3 helices (-) : Score = 9.59013 4 helices (+) : Score = 11.781 4 helices (-) : Score = 11.6086 5 helices (+) : Score = 13.2301 5 helices (-) : Score = 13.4324 6 helices (+) : Score = 14.1968 6 helices (-) : Score = 14.0016 7 helices (+) : Score = 13.5246 7 helices (-) : Score = 13.7432 8 helices (+) : Score = 13.0574 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.844 Topology: 17-32,37-60,68-89,98-115,129-155,186-204 Re-entrant helices: Not detected. Helix count: 6 N-terminal: in Score: 14.1968