Processing 1 helix: Transmembrane helix 1 from 42 (in) to 57 (out) : -191.742 Score = -0.147620 Processing 1 helix: Transmembrane helix 1 from 42 (out) to 57 (in) : -211.458 Score = -0.255580 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -304.152 Transmembrane helix 2 from 23 (out) to 38 (in) : -613.287 Transmembrane helix 3 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.747 Transmembrane helix 5 from 80 (in) to 95 (out) : -691.872 Transmembrane helix 6 from 100 (out) to 115 (in) : -530.181 Transmembrane helix 7 from 119 (in) to 134 (out) : -502.808 Transmembrane helix 8 from 143 (out) to 158 (in) : -1485 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 23 (in) to 38 (out) : -603.513 Transmembrane helix 3 from 42 (out) to 57 (in) : -211.458 Transmembrane helix 4 from 61 (in) to 76 (out) : -313.853 Transmembrane helix 5 from 80 (out) to 95 (in) : -691.828 Transmembrane helix 6 from 99 (in) to 114 (out) : -573.083 Transmembrane helix 7 from 118 (out) to 133 (in) : -472.731 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -304.152 Transmembrane helix 2 from 29 (out) to 44 (in) : -470.01 Transmembrane helix 3 from 48 (in) to 63 (out) : -213.832 Transmembrane helix 4 from 67 (out) to 82 (in) : -308.931 Transmembrane helix 5 from 98 (in) to 113 (out) : -605.118 Transmembrane helix 6 from 117 (out) to 132 (in) : -438.947 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 31 (in) to 46 (out) : -430.438 Transmembrane helix 3 from 50 (out) to 65 (in) : -220.825 Transmembrane helix 4 from 69 (in) to 84 (out) : -337.56 Transmembrane helix 5 from 111 (out) to 126 (in) : -326.185 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -304.152 Transmembrane helix 2 from 42 (out) to 57 (in) : -211.458 Transmembrane helix 3 from 65 (in) to 80 (out) : -295.158 Transmembrane helix 4 from 111 (out) to 126 (in) : -326.185 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 3 from 111 (out) to 126 (in) : -326.185 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 2 from 111 (out) to 126 (in) : -326.185 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 23 (in) to 38 (out) : -603.513 Transmembrane helix 3 from 42 (out) to 57 (in) : -211.458 Transmembrane helix 4 from 61 (in) to 76 (out) : -313.853 Transmembrane helix 5 from 80 (out) to 95 (in) : -691.828 Transmembrane helix 6 from 99 (in) to 114 (out) : -573.083 Transmembrane helix 7 from 118 (out) to 133 (in) : -472.731 Transmembrane helix 8 from 143 (in) to 158 (out) : -1476.8 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -304.152 Transmembrane helix 2 from 23 (out) to 38 (in) : -613.287 Transmembrane helix 3 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.747 Transmembrane helix 5 from 80 (in) to 95 (out) : -691.872 Transmembrane helix 6 from 100 (out) to 115 (in) : -530.181 Transmembrane helix 7 from 119 (in) to 134 (out) : -502.808 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 31 (in) to 46 (out) : -430.438 Transmembrane helix 3 from 50 (out) to 65 (in) : -220.825 Transmembrane helix 4 from 69 (in) to 84 (out) : -337.56 Transmembrane helix 5 from 100 (out) to 115 (in) : -530.181 Transmembrane helix 6 from 119 (in) to 134 (out) : -502.808 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -304.152 Transmembrane helix 2 from 29 (out) to 44 (in) : -470.01 Transmembrane helix 3 from 48 (in) to 63 (out) : -213.832 Transmembrane helix 4 from 67 (out) to 82 (in) : -308.931 Transmembrane helix 5 from 111 (in) to 126 (out) : -334.515 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 3 from 65 (out) to 80 (in) : -295.542 Transmembrane helix 4 from 111 (in) to 126 (out) : -334.515 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 42 (in) to 57 (out) : -191.742 Transmembrane helix 2 from 65 (out) to 80 (in) : -295.542 Transmembrane helix 3 from 111 (in) to 126 (out) : -334.515 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -293.248 Transmembrane helix 2 from 42 (in) to 57 (out) : -191.742 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.14762 1 helix (-) : Score = -0.25558 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 42-57 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: -0.14762