BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (125 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64440 Inner membrane protein ybjM n=102 Tax=Enterobact...   249   3e-65
UniRef50_B7LN14 Putative uncharacterized protein ybjM n=1 Tax=Es...   199   2e-50
UniRef50_C6DEP3 Putative uncharacterized protein n=8 Tax=Enterob...   104   7e-22
UniRef50_C9Y011 Inner membrane protein ybjM n=1 Tax=Cronobacter ...   100   1e-20
UniRef50_D1RNZ3 Putative uncharacterized protein n=1 Tax=Serrati...    99   6e-20
UniRef50_A8GCA4 Putative uncharacterized protein n=1 Tax=Serrati...    93   2e-18
UniRef50_A7MF40 Putative uncharacterized protein n=1 Tax=Cronoba...    91   1e-17
UniRef50_C5BED8 Putative uncharacterized protein n=2 Tax=Edwards...    53   3e-06

>UniRef50_P64440 Inner membrane protein ybjM n=102 Tax=Enterobacteriaceae
           RepID=YBJM_ECO57
          Length = 125

 Score =  249 bits (635), Expect = 3e-65,   Method: Compositional matrix adjust.
 Identities = 125/125 (100%), Positives = 125/125 (100%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE
Sbjct: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120
           VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ
Sbjct: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120

Query: 121 HRKNQ 125
           HRKNQ
Sbjct: 121 HRKNQ 125


>UniRef50_B7LN14 Putative uncharacterized protein ybjM n=1 Tax=Escherichia
           fergusonii ATCC 35469 RepID=B7LN14_ESCF3
          Length = 129

 Score =  199 bits (506), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 98/125 (78%), Positives = 109/125 (87%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           MK K+ WAGAICCFVLFIVVCLFLAT MKGAFRAAGHPE+GLLFFILPGAVASF S RR 
Sbjct: 1   MKRKRSWAGAICCFVLFIVVCLFLATQMKGAFRAAGHPELGLLFFILPGAVASFCSNRRA 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120
           V+ PL GAM+AAPC ML++RLFF+  RS WQELAWLLSAVFWCALGALCFLFISSLF  +
Sbjct: 61  VINPLLGAMMAAPCCMLLLRLFFTTHRSLWQELAWLLSAVFWCALGALCFLFISSLFDLR 120

Query: 121 HRKNQ 125
            ++ +
Sbjct: 121 QKRER 125


>UniRef50_C6DEP3 Putative uncharacterized protein n=8 Tax=Enterobacteriaceae
           RepID=C6DEP3_PECCP
          Length = 123

 Score =  104 bits (260), Expect = 7e-22,   Method: Compositional matrix adjust.
 Identities = 59/127 (46%), Positives = 80/127 (62%), Gaps = 9/127 (7%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAA-----GHPEIGLLFFILPGAVASFF 55
           M   + W GA CCF+LF VV  FL+  ++ +         G P  G+L F+LPG VA F 
Sbjct: 1   MAKNKGWVGATCCFLLFTVV--FLSQKIEASDVVVNDGLRGSP--GMLLFLLPGMVACFL 56

Query: 56  SQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISS 115
           S R  +L PLFGA+ A P  +L++ L+ +P RSFWQELA+++SAVFWC LGAL  L + S
Sbjct: 57  SARGRLLYPLFGALAAMPVCLLVLHLWNTPMRSFWQELAYVMSAVFWCVLGALGVLCLRS 116

Query: 116 LFKPQHR 122
           L++   R
Sbjct: 117 LYRRYLR 123


>UniRef50_C9Y011 Inner membrane protein ybjM n=1 Tax=Cronobacter turicensis
           RepID=C9Y011_CROTZ
          Length = 125

 Score =  100 bits (249), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 55/118 (46%), Positives = 79/118 (66%), Gaps = 5/118 (4%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAF--RAAGHPEIGLLFFILPGAVASFFSQR 58
           M+   R A  +CCF+LF+ V  FLA  M         G  ++G+L+F+LPGAVA   ++R
Sbjct: 1   MRRHSRTA-TVCCFILFLAV--FLAQKMVPGTLPPVCGSMDLGILYFMLPGAVAGLCARR 57

Query: 59  REVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL 116
              LKPL GA+LAAP  ++I+ L+    RS WQELAW+ SA+FWC++G+LC+LF+ +L
Sbjct: 58  GRELKPLLGALLAAPICLVILHLWSGEGRSIWQELAWIFSALFWCSIGSLCWLFVLTL 115


>UniRef50_D1RNZ3 Putative uncharacterized protein n=1 Tax=Serratia odorifera 4Rx13
           RepID=D1RNZ3_SEROD
          Length = 129

 Score = 98.6 bits (244), Expect = 6e-20,   Method: Compositional matrix adjust.
 Identities = 52/128 (40%), Positives = 75/128 (58%), Gaps = 3/128 (2%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           M + + W G + C +LF +V L   +   G+     H E GLL F++PG +AS+ S ++ 
Sbjct: 1   MANYRYWVGILSCSLLFSLVFLGQQSGAFGSTDHEHHGETGLLLFVIPGMIASYLSSKKR 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL---F 117
           +L PL GA+ A P  + I   + +P  SFWQELA+  SAVFWC  GA+  LF  SL   F
Sbjct: 61  ILCPLLGALYALPLCLTIRHFWLTPFYSFWQELAYATSAVFWCVFGAMLALFARSLLQAF 120

Query: 118 KPQHRKNQ 125
           +P HR+ +
Sbjct: 121 QPGHRRER 128


>UniRef50_A8GCA4 Putative uncharacterized protein n=1 Tax=Serratia proteamaculans
           568 RepID=A8GCA4_SERP5
          Length = 126

 Score = 93.2 bits (230), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 51/121 (42%), Positives = 71/121 (58%), Gaps = 6/121 (4%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHP---EIGLLFFILPGAVASFFSQ 57
           M  ++ W G + CF+LF +V L       GAF +  H    E GLL F++PG VAS+ S 
Sbjct: 1   MASERYWWGILSCFLLFSLVYL---GQQSGAFGSTDHEHRGETGLLLFVIPGVVASYLSS 57

Query: 58  RREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLF 117
           ++ +L PL GA+ A P  ++I   + +P+ S WQELA+  SAVFWC  GAL  LF   L 
Sbjct: 58  KKRILCPLLGALYALPLCLIIRHFWLTPSASVWQELAYATSAVFWCMFGALLALFARGLL 117

Query: 118 K 118
           +
Sbjct: 118 Q 118


>UniRef50_A7MF40 Putative uncharacterized protein n=1 Tax=Cronobacter sakazakii ATCC
           BAA-894 RepID=A7MF40_ENTS8
          Length = 105

 Score = 90.9 bits (224), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 44/92 (47%), Positives = 67/92 (72%), Gaps = 3/92 (3%)

Query: 34  AAGHPEIGLLFFILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQEL 93
            AG  ++G+L F+LPGAVA   ++R   LKPL GA++AAP  ++++ L+    RSFWQEL
Sbjct: 13  GAGTMDLGILSFMLPGAVAGVCARRGRELKPLAGALIAAPLCLVLLHLWGGEARSFWQEL 72

Query: 94  AWLLSAVFWCALGALCFLFISSLFKPQHRKNQ 125
           AW+ SA+FWC++G+LC+LF+ +L   +HR + 
Sbjct: 73  AWIFSALFWCSIGSLCWLFLITL---RHRGDS 101


>UniRef50_C5BED8 Putative uncharacterized protein n=2 Tax=Edwardsiella
           RepID=C5BED8_EDWI9
          Length = 125

 Score = 53.1 bits (126), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 38/120 (31%), Positives = 60/120 (50%), Gaps = 6/120 (5%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATH----MKGAFRAAGHPEIGLLFFILPGAVASFFS 56
           M + +RW   I CF++  V  LFL +H      G          GLL ++LPG +    +
Sbjct: 1   MANGRRWFTLILCFLM--VTTLFLLSHCYVQAHGGIAEPPGMRPGLLLYMLPGFILCPLA 58

Query: 57  QRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL 116
           +R+ VL    GA+++A    L+  L+ +   +  Q L +  S VFWC  GAL + F ++L
Sbjct: 59  RRQRVLTVFCGALVSAVFCQLLHMLWLADDETPLQLLPYTTSMVFWCVFGALLYWFAATL 118


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P64440 Inner membrane protein ybjM n=102 Tax=Enterobact...   150   1e-35
UniRef50_B7LN14 Putative uncharacterized protein ybjM n=1 Tax=Es...   148   4e-35
UniRef50_D1RNZ3 Putative uncharacterized protein n=1 Tax=Serrati...   145   3e-34
UniRef50_A8GCA4 Putative uncharacterized protein n=1 Tax=Serrati...   136   2e-31
UniRef50_C6DEP3 Putative uncharacterized protein n=8 Tax=Enterob...   124   9e-28
UniRef50_C9Y011 Inner membrane protein ybjM n=1 Tax=Cronobacter ...   116   3e-25
UniRef50_C5BED8 Putative uncharacterized protein n=2 Tax=Edwards...   115   5e-25
UniRef50_A7MF40 Putative uncharacterized protein n=1 Tax=Cronoba...   110   2e-23

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P64440 Inner membrane protein ybjM n=102 Tax=Enterobacteriaceae
           RepID=YBJM_ECO57
          Length = 125

 Score =  150 bits (380), Expect = 1e-35,   Method: Composition-based stats.
 Identities = 125/125 (100%), Positives = 125/125 (100%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE
Sbjct: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120
           VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ
Sbjct: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120

Query: 121 HRKNQ 125
           HRKNQ
Sbjct: 121 HRKNQ 125


>UniRef50_B7LN14 Putative uncharacterized protein ybjM n=1 Tax=Escherichia
           fergusonii ATCC 35469 RepID=B7LN14_ESCF3
          Length = 129

 Score =  148 bits (374), Expect = 4e-35,   Method: Composition-based stats.
 Identities = 98/125 (78%), Positives = 109/125 (87%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           MK K+ WAGAICCFVLFIVVCLFLAT MKGAFRAAGHPE+GLLFFILPGAVASF S RR 
Sbjct: 1   MKRKRSWAGAICCFVLFIVVCLFLATQMKGAFRAAGHPELGLLFFILPGAVASFCSNRRA 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120
           V+ PL GAM+AAPC ML++RLFF+  RS WQELAWLLSAVFWCALGALCFLFISSLF  +
Sbjct: 61  VINPLLGAMMAAPCCMLLLRLFFTTHRSLWQELAWLLSAVFWCALGALCFLFISSLFDLR 120

Query: 121 HRKNQ 125
            ++ +
Sbjct: 121 QKRER 125


>UniRef50_D1RNZ3 Putative uncharacterized protein n=1 Tax=Serratia odorifera 4Rx13
           RepID=D1RNZ3_SEROD
          Length = 129

 Score =  145 bits (367), Expect = 3e-34,   Method: Composition-based stats.
 Identities = 52/128 (40%), Positives = 75/128 (58%), Gaps = 3/128 (2%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           M + + W G + C +LF +V L   +   G+     H E GLL F++PG +AS+ S ++ 
Sbjct: 1   MANYRYWVGILSCSLLFSLVFLGQQSGAFGSTDHEHHGETGLLLFVIPGMIASYLSSKKR 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL---F 117
           +L PL GA+ A P  + I   + +P  SFWQELA+  SAVFWC  GA+  LF  SL   F
Sbjct: 61  ILCPLLGALYALPLCLTIRHFWLTPFYSFWQELAYATSAVFWCVFGAMLALFARSLLQAF 120

Query: 118 KPQHRKNQ 125
           +P HR+ +
Sbjct: 121 QPGHRRER 128


>UniRef50_A8GCA4 Putative uncharacterized protein n=1 Tax=Serratia proteamaculans
           568 RepID=A8GCA4_SERP5
          Length = 126

 Score =  136 bits (343), Expect = 2e-31,   Method: Composition-based stats.
 Identities = 48/125 (38%), Positives = 72/125 (57%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLFFILPGAVASFFSQRRE 60
           M  ++ W G + CF+LF +V L   +   G+       E GLL F++PG VAS+ S ++ 
Sbjct: 1   MASERYWWGILSCFLLFSLVYLGQQSGAFGSTDHEHRGETGLLLFVIPGVVASYLSSKKR 60

Query: 61  VLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQ 120
           +L PL GA+ A P  ++I   + +P+ S WQELA+  SAVFWC  GAL  LF   L +  
Sbjct: 61  ILCPLLGALYALPLCLIIRHFWLTPSASVWQELAYATSAVFWCMFGALLALFARGLLQAY 120

Query: 121 HRKNQ 125
            + ++
Sbjct: 121 RQCHR 125


>UniRef50_C6DEP3 Putative uncharacterized protein n=8 Tax=Enterobacteriaceae
           RepID=C6DEP3_PECCP
          Length = 123

 Score =  124 bits (311), Expect = 9e-28,   Method: Composition-based stats.
 Identities = 57/125 (45%), Positives = 78/125 (62%), Gaps = 5/125 (4%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAG---HPEIGLLFFILPGAVASFFSQ 57
           M   + W GA CCF+LF VV  FL+  ++ +            G+L F+LPG VA F S 
Sbjct: 1   MAKNKGWVGATCCFLLFTVV--FLSQKIEASDVVVNDGLRGSPGMLLFLLPGMVACFLSA 58

Query: 58  RREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLF 117
           R  +L PLFGA+ A P  +L++ L+ +P RSFWQELA+++SAVFWC LGAL  L + SL+
Sbjct: 59  RGRLLYPLFGALAAMPVCLLVLHLWNTPMRSFWQELAYVMSAVFWCVLGALGVLCLRSLY 118

Query: 118 KPQHR 122
           +   R
Sbjct: 119 RRYLR 123


>UniRef50_C9Y011 Inner membrane protein ybjM n=1 Tax=Cronobacter turicensis
           RepID=C9Y011_CROTZ
          Length = 125

 Score =  116 bits (290), Expect = 3e-25,   Method: Composition-based stats.
 Identities = 55/118 (46%), Positives = 79/118 (66%), Gaps = 5/118 (4%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATHMKGAF--RAAGHPEIGLLFFILPGAVASFFSQR 58
           M+   R A  +CCF+LF+ V  FLA  M         G  ++G+L+F+LPGAVA   ++R
Sbjct: 1   MRRHSRTAT-VCCFILFLAV--FLAQKMVPGTLPPVCGSMDLGILYFMLPGAVAGLCARR 57

Query: 59  REVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL 116
              LKPL GA+LAAP  ++I+ L+    RS WQELAW+ SA+FWC++G+LC+LF+ +L
Sbjct: 58  GRELKPLLGALLAAPICLVILHLWSGEGRSIWQELAWIFSALFWCSIGSLCWLFVLTL 115


>UniRef50_C5BED8 Putative uncharacterized protein n=2 Tax=Edwardsiella
           RepID=C5BED8_EDWI9
          Length = 125

 Score =  115 bits (288), Expect = 5e-25,   Method: Composition-based stats.
 Identities = 38/120 (31%), Positives = 60/120 (50%), Gaps = 6/120 (5%)

Query: 1   MKHKQRWAGAICCFVLFIVVCLFLATH----MKGAFRAAGHPEIGLLFFILPGAVASFFS 56
           M + +RW   I CF++  V  LFL +H      G          GLL ++LPG +    +
Sbjct: 1   MANGRRWFTLILCFLM--VTTLFLLSHCYVQAHGGIAEPPGMRPGLLLYMLPGFILCPLA 58

Query: 57  QRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFWCALGALCFLFISSL 116
           +R+ VL    GA+++A    L+  L+ +   +  Q L +  S VFWC  GAL + F ++L
Sbjct: 59  RRQRVLTVFCGALVSAVFCQLLHMLWLADDETPLQLLPYTTSMVFWCVFGALLYWFAATL 118


>UniRef50_A7MF40 Putative uncharacterized protein n=1 Tax=Cronobacter sakazakii ATCC
           BAA-894 RepID=A7MF40_ENTS8
          Length = 105

 Score =  110 bits (274), Expect = 2e-23,   Method: Composition-based stats.
 Identities = 44/104 (42%), Positives = 68/104 (65%), Gaps = 2/104 (1%)

Query: 24  LATHMKGAF--RAAGHPEIGLLFFILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRL 81
           +A  M        AG  ++G+L F+LPGAVA   ++R   LKPL GA++AAP  ++++ L
Sbjct: 1   MAQKMVPGTFAPGAGTMDLGILSFMLPGAVAGVCARRGRELKPLAGALIAAPLCLVLLHL 60

Query: 82  FFSPTRSFWQELAWLLSAVFWCALGALCFLFISSLFKPQHRKNQ 125
           +    RSFWQELAW+ SA+FWC++G+LC+LF+ +L      + +
Sbjct: 61  WGGEARSFWQELAWIFSALFWCSIGSLCWLFLITLRHRGDSRRR 104


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.323    0.138    0.432 

Lambda     K      H
   0.267   0.0427    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 516,285,105
Number of Sequences: 3077464
Number of extensions: 19799217
Number of successful extensions: 103272
Number of sequences better than 1.0e-01: 14
Number of HSP's better than  0.1 without gapping: 17
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 103242
Number of HSP's gapped (non-prelim): 26
length of query: 125
length of database: 1,040,396,356
effective HSP length: 91
effective length of query: 34
effective length of database: 760,347,132
effective search space: 25851802488
effective search space used: 25851802488
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.0 bits)
S2: 87 (38.1 bits)
