Processing 1 helix: Transmembrane helix 1 from 109 (in) to 124 (out) : 106.596 Score = 0.106596 Processing 1 helix: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Score = 0.094504 Processing 21 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -21839.4 Transmembrane helix 2 from 23 (out) to 38 (in) : -429.505 Transmembrane helix 3 from 42 (in) to 57 (out) : -413.434 Transmembrane helix 4 from 61 (out) to 76 (in) : -325.888 Transmembrane helix 5 from 80 (in) to 95 (out) : -198.047 Transmembrane helix 6 from 99 (out) to 114 (in) : -112.775 Transmembrane helix 7 from 118 (in) to 133 (out) : -152.002 Transmembrane helix 8 from 137 (out) to 152 (in) : -271.036 Transmembrane helix 9 from 156 (in) to 171 (out) : -282.009 Transmembrane helix 10 from 175 (out) to 190 (in) : -119.431 Transmembrane helix 11 from 194 (in) to 209 (out) : -172.922 Transmembrane helix 12 from 213 (out) to 228 (in) : -478.3 Transmembrane helix 13 from 232 (in) to 247 (out) : -594.383 Transmembrane helix 14 from 251 (out) to 266 (in) : -193.795 Transmembrane helix 15 from 270 (in) to 285 (out) : -668.978 Transmembrane helix 16 from 289 (out) to 304 (in) : -738.512 Transmembrane helix 17 from 308 (in) to 323 (out) : -959.689 Transmembrane helix 18 from 327 (out) to 342 (in) : -448.596 Transmembrane helix 19 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 20 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 21 from 384 (in) to 399 (out) : 30.927 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -450.672 Transmembrane helix 2 from 40 (in) to 55 (out) : -381.351 Transmembrane helix 3 from 59 (out) to 74 (in) : -380.716 Transmembrane helix 4 from 78 (in) to 93 (out) : -177.295 Transmembrane helix 5 from 97 (out) to 112 (in) : -162.864 Transmembrane helix 6 from 116 (in) to 131 (out) : -52.876 Transmembrane helix 7 from 135 (out) to 150 (in) : -352.759 Transmembrane helix 8 from 154 (in) to 169 (out) : -316.443 Transmembrane helix 9 from 173 (out) to 188 (in) : -103.202 Transmembrane helix 10 from 192 (in) to 207 (out) : -175.874 Transmembrane helix 11 from 211 (out) to 226 (in) : -344.541 Transmembrane helix 12 from 232 (in) to 247 (out) : -594.383 Transmembrane helix 13 from 251 (out) to 266 (in) : -193.795 Transmembrane helix 14 from 270 (in) to 285 (out) : -668.978 Transmembrane helix 15 from 289 (out) to 304 (in) : -738.512 Transmembrane helix 16 from 308 (in) to 323 (out) : -959.689 Transmembrane helix 17 from 327 (out) to 342 (in) : -448.596 Transmembrane helix 18 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 19 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 20 from 384 (in) to 399 (out) : 30.927 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -458.328 Transmembrane helix 2 from 40 (out) to 55 (in) : -386.849 Transmembrane helix 3 from 59 (in) to 74 (out) : -380.184 Transmembrane helix 4 from 78 (out) to 93 (in) : -184.205 Transmembrane helix 5 from 97 (in) to 112 (out) : -173.636 Transmembrane helix 6 from 116 (out) to 131 (in) : -51.824 Transmembrane helix 7 from 138 (in) to 153 (out) : -232.966 Transmembrane helix 8 from 157 (out) to 172 (in) : -246.634 Transmembrane helix 9 from 176 (in) to 191 (out) : -131.686 Transmembrane helix 10 from 195 (out) to 210 (in) : -170.349 Transmembrane helix 11 from 214 (in) to 229 (out) : -546.561 Transmembrane helix 12 from 235 (out) to 250 (in) : -464.593 Transmembrane helix 13 from 254 (in) to 269 (out) : -257.733 Transmembrane helix 14 from 273 (out) to 288 (in) : -691.311 Transmembrane helix 15 from 295 (in) to 310 (out) : -699.229 Transmembrane helix 16 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 17 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 18 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 19 from 384 (in) to 399 (out) : 30.927 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -450.672 Transmembrane helix 2 from 40 (in) to 55 (out) : -381.351 Transmembrane helix 3 from 59 (out) to 74 (in) : -380.716 Transmembrane helix 4 from 78 (in) to 93 (out) : -177.295 Transmembrane helix 5 from 97 (out) to 112 (in) : -162.864 Transmembrane helix 6 from 116 (in) to 131 (out) : -52.876 Transmembrane helix 7 from 135 (out) to 150 (in) : -352.759 Transmembrane helix 8 from 154 (in) to 169 (out) : -316.443 Transmembrane helix 9 from 173 (out) to 188 (in) : -103.202 Transmembrane helix 10 from 192 (in) to 207 (out) : -175.874 Transmembrane helix 11 from 211 (out) to 226 (in) : -344.541 Transmembrane helix 12 from 239 (in) to 254 (out) : -294.072 Transmembrane helix 13 from 258 (out) to 273 (in) : -377.138 Transmembrane helix 14 from 295 (in) to 310 (out) : -699.229 Transmembrane helix 15 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 16 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 17 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 18 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 277 (in) to 291 (in) : 1050 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -458.328 Transmembrane helix 2 from 40 (out) to 55 (in) : -386.849 Transmembrane helix 3 from 59 (in) to 74 (out) : -380.184 Transmembrane helix 4 from 78 (out) to 93 (in) : -184.205 Transmembrane helix 5 from 97 (in) to 112 (out) : -173.636 Transmembrane helix 6 from 116 (out) to 131 (in) : -51.824 Transmembrane helix 7 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 8 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 9 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 10 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 11 from 239 (in) to 254 (out) : -294.072 Transmembrane helix 12 from 258 (out) to 273 (in) : -377.138 Transmembrane helix 13 from 295 (in) to 310 (out) : -699.229 Transmembrane helix 14 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 15 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 16 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 17 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 277 (in) to 291 (in) : 1050 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -450.672 Transmembrane helix 2 from 40 (in) to 55 (out) : -381.351 Transmembrane helix 3 from 59 (out) to 74 (in) : -380.716 Transmembrane helix 4 from 78 (in) to 93 (out) : -177.295 Transmembrane helix 5 from 97 (out) to 112 (in) : -162.864 Transmembrane helix 6 from 116 (in) to 131 (out) : -52.876 Transmembrane helix 7 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 8 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 9 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 10 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 11 from 239 (out) to 254 (in) : -298.528 Transmembrane helix 12 from 258 (in) to 273 (out) : -364.962 Transmembrane helix 13 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 14 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 15 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 16 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 277 (out) to 311 (out) : 2450 Re-entrant helix 2 from 312 (out) to 320 (out) : 630 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -343.177 Transmembrane helix 2 from 56 (out) to 71 (in) : -439.835 Transmembrane helix 3 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 4 from 94 (out) to 109 (in) : -220.267 Transmembrane helix 5 from 113 (in) to 128 (out) : 65.884 Transmembrane helix 6 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 7 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 8 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 9 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 10 from 239 (out) to 254 (in) : -298.528 Transmembrane helix 11 from 258 (in) to 273 (out) : -364.962 Transmembrane helix 12 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 13 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 14 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 15 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 277 (out) to 311 (out) : 2450 Re-entrant helix 2 from 312 (out) to 320 (out) : 630 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -348.061 Transmembrane helix 2 from 56 (in) to 71 (out) : -431.165 Transmembrane helix 3 from 75 (out) to 90 (in) : -161.101 Transmembrane helix 4 from 94 (in) to 109 (out) : -220.133 Transmembrane helix 5 from 113 (out) to 128 (in) : 58.816 Transmembrane helix 6 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 7 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 8 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 9 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 10 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 11 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 12 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 13 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 14 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 320 (out) : 1400 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -343.177 Transmembrane helix 2 from 74 (out) to 89 (in) : -159.998 Transmembrane helix 3 from 93 (in) to 108 (out) : -233.478 Transmembrane helix 4 from 112 (out) to 127 (in) : 79.94 Transmembrane helix 5 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 6 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 7 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 8 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 9 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 10 from 324 (out) to 339 (in) : -428.906 Transmembrane helix 11 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 12 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 13 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 320 (out) : 1400 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -348.061 Transmembrane helix 2 from 74 (in) to 89 (out) : -150.302 Transmembrane helix 3 from 93 (out) to 108 (in) : -238.322 Transmembrane helix 4 from 112 (in) to 127 (out) : 87.86 Transmembrane helix 5 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 6 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 7 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 8 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 9 from 249 (out) to 264 (in) : -183.576 Transmembrane helix 10 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 11 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 12 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (out) to 245 (out) : 630 Re-entrant helix 2 from 268 (in) to 302 (in) : 2450 Re-entrant helix 3 from 303 (in) to 337 (in) : 2450 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 74 (in) to 89 (out) : -150.302 Transmembrane helix 2 from 93 (out) to 108 (in) : -238.322 Transmembrane helix 3 from 112 (in) to 127 (out) : 87.86 Transmembrane helix 4 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 5 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 6 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 7 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 8 from 249 (out) to 264 (in) : -183.576 Transmembrane helix 9 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 10 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 11 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (out) to 245 (out) : 630 Re-entrant helix 2 from 268 (in) to 302 (in) : 2450 Re-entrant helix 3 from 303 (in) to 337 (in) : 2450 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 74 (out) to 89 (in) : -159.998 Transmembrane helix 2 from 109 (in) to 124 (out) : 106.596 Transmembrane helix 3 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 4 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 5 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 6 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 7 from 249 (out) to 264 (in) : -183.576 Transmembrane helix 8 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 9 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 10 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (out) to 245 (out) : 630 Re-entrant helix 2 from 268 (in) to 302 (in) : 2450 Re-entrant helix 3 from 303 (in) to 337 (in) : 2450 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 4 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 5 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 6 from 249 (out) to 264 (in) : -183.576 Transmembrane helix 7 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 8 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 9 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (out) to 245 (out) : 630 Re-entrant helix 2 from 268 (in) to 302 (in) : 2450 Re-entrant helix 3 from 303 (in) to 337 (in) : 2450 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 3 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 4 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 5 from 249 (out) to 264 (in) : -183.576 Transmembrane helix 6 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 7 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 8 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 224 (out) to 245 (out) : 630 Re-entrant helix 2 from 268 (in) to 302 (in) : 2450 Re-entrant helix 3 from 303 (in) to 337 (in) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 169 (in) to 184 (out) : -80.068 Transmembrane helix 4 from 201 (out) to 216 (in) : -139.219 Transmembrane helix 5 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 6 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 7 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 169 (in) to 184 (out) : -80.068 Transmembrane helix 3 from 201 (out) to 216 (in) : -139.219 Transmembrane helix 4 from 346 (in) to 361 (out) : -110.556 Transmembrane helix 5 from 365 (out) to 380 (in) : -106.93 Transmembrane helix 6 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 168 (in) to 183 (out) : -78.179 Transmembrane helix 4 from 357 (out) to 372 (in) : -42.98 Transmembrane helix 5 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Re-entrant helix 4 from 342 (out) to 353 (out) : 840 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 168 (in) to 183 (out) : -78.179 Transmembrane helix 3 from 357 (out) to 372 (in) : -42.98 Transmembrane helix 4 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Re-entrant helix 4 from 342 (out) to 353 (out) : 840 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 109 (in) to 124 (out) : 106.596 Transmembrane helix 2 from 357 (out) to 372 (in) : -42.98 Transmembrane helix 3 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Re-entrant helix 4 from 342 (out) to 353 (out) : 840 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 384 (in) to 399 (out) : 30.927 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Re-entrant helix 4 from 342 (in) to 376 (in) : 2450 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -21818 Transmembrane helix 2 from 23 (in) to 38 (out) : -430.495 Transmembrane helix 3 from 42 (out) to 57 (in) : -421.766 Transmembrane helix 4 from 61 (in) to 76 (out) : -334.512 Transmembrane helix 5 from 80 (out) to 95 (in) : -202.353 Transmembrane helix 6 from 99 (in) to 114 (out) : -129.825 Transmembrane helix 7 from 118 (out) to 133 (in) : -147.898 Transmembrane helix 8 from 137 (in) to 152 (out) : -269.964 Transmembrane helix 9 from 156 (out) to 171 (in) : -271.191 Transmembrane helix 10 from 175 (in) to 190 (out) : -135.669 Transmembrane helix 11 from 194 (out) to 209 (in) : -175.278 Transmembrane helix 12 from 213 (in) to 228 (out) : -484.4 Transmembrane helix 13 from 232 (out) to 247 (in) : -604.517 Transmembrane helix 14 from 251 (in) to 266 (out) : -210.205 Transmembrane helix 15 from 270 (out) to 285 (in) : -668.822 Transmembrane helix 16 from 289 (in) to 304 (out) : -730.188 Transmembrane helix 17 from 308 (out) to 323 (in) : -952.111 Transmembrane helix 18 from 327 (in) to 342 (out) : -452.304 Transmembrane helix 19 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 20 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 21 from 384 (out) to 399 (in) : 28.473 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -458.328 Transmembrane helix 2 from 40 (out) to 55 (in) : -386.849 Transmembrane helix 3 from 59 (in) to 74 (out) : -380.184 Transmembrane helix 4 from 78 (out) to 93 (in) : -184.205 Transmembrane helix 5 from 97 (in) to 112 (out) : -173.636 Transmembrane helix 6 from 116 (out) to 131 (in) : -51.824 Transmembrane helix 7 from 135 (in) to 150 (out) : -355.041 Transmembrane helix 8 from 154 (out) to 169 (in) : -310.257 Transmembrane helix 9 from 173 (in) to 188 (out) : -117.098 Transmembrane helix 10 from 192 (out) to 207 (in) : -176.126 Transmembrane helix 11 from 211 (in) to 226 (out) : -359.859 Transmembrane helix 12 from 232 (out) to 247 (in) : -604.517 Transmembrane helix 13 from 251 (in) to 266 (out) : -210.205 Transmembrane helix 14 from 270 (out) to 285 (in) : -668.822 Transmembrane helix 15 from 289 (in) to 304 (out) : -730.188 Transmembrane helix 16 from 308 (out) to 323 (in) : -952.111 Transmembrane helix 17 from 327 (in) to 342 (out) : -452.304 Transmembrane helix 18 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 19 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 20 from 384 (out) to 399 (in) : 28.473 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -450.672 Transmembrane helix 2 from 40 (in) to 55 (out) : -381.351 Transmembrane helix 3 from 59 (out) to 74 (in) : -380.716 Transmembrane helix 4 from 78 (in) to 93 (out) : -177.295 Transmembrane helix 5 from 97 (out) to 112 (in) : -162.864 Transmembrane helix 6 from 116 (in) to 131 (out) : -52.876 Transmembrane helix 7 from 135 (out) to 150 (in) : -352.759 Transmembrane helix 8 from 154 (in) to 169 (out) : -316.443 Transmembrane helix 9 from 173 (out) to 188 (in) : -103.202 Transmembrane helix 10 from 192 (in) to 207 (out) : -175.874 Transmembrane helix 11 from 211 (out) to 226 (in) : -344.541 Transmembrane helix 12 from 235 (in) to 250 (out) : -452.507 Transmembrane helix 13 from 254 (out) to 269 (in) : -255.367 Transmembrane helix 14 from 273 (in) to 288 (out) : -682.889 Transmembrane helix 15 from 298 (out) to 313 (in) : -698.059 Transmembrane helix 16 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 17 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 18 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 19 from 384 (out) to 399 (in) : 28.473 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -458.328 Transmembrane helix 2 from 40 (out) to 55 (in) : -386.849 Transmembrane helix 3 from 59 (in) to 74 (out) : -380.184 Transmembrane helix 4 from 78 (out) to 93 (in) : -184.205 Transmembrane helix 5 from 97 (in) to 112 (out) : -173.636 Transmembrane helix 6 from 116 (out) to 131 (in) : -51.824 Transmembrane helix 7 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 8 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 9 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 10 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 11 from 235 (in) to 250 (out) : -452.507 Transmembrane helix 12 from 254 (out) to 269 (in) : -255.367 Transmembrane helix 13 from 273 (in) to 288 (out) : -682.889 Transmembrane helix 14 from 298 (out) to 313 (in) : -698.059 Transmembrane helix 15 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 16 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 17 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 18 from 384 (out) to 399 (in) : 28.473 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -450.672 Transmembrane helix 2 from 40 (in) to 55 (out) : -381.351 Transmembrane helix 3 from 59 (out) to 74 (in) : -380.716 Transmembrane helix 4 from 78 (in) to 93 (out) : -177.295 Transmembrane helix 5 from 97 (out) to 112 (in) : -162.864 Transmembrane helix 6 from 116 (in) to 131 (out) : -52.876 Transmembrane helix 7 from 135 (out) to 150 (in) : -352.759 Transmembrane helix 8 from 154 (in) to 169 (out) : -316.443 Transmembrane helix 9 from 173 (out) to 188 (in) : -103.202 Transmembrane helix 10 from 192 (in) to 207 (out) : -175.874 Transmembrane helix 11 from 211 (out) to 226 (in) : -344.541 Transmembrane helix 12 from 239 (in) to 254 (out) : -294.072 Transmembrane helix 13 from 258 (out) to 273 (in) : -377.138 Transmembrane helix 14 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 15 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 16 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 17 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 277 (in) to 311 (in) : 2450 Re-entrant helix 2 from 312 (in) to 320 (in) : 630 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -458.328 Transmembrane helix 2 from 40 (out) to 55 (in) : -386.849 Transmembrane helix 3 from 59 (in) to 74 (out) : -380.184 Transmembrane helix 4 from 78 (out) to 93 (in) : -184.205 Transmembrane helix 5 from 97 (in) to 112 (out) : -173.636 Transmembrane helix 6 from 116 (out) to 131 (in) : -51.824 Transmembrane helix 7 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 8 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 9 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 10 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 11 from 239 (in) to 254 (out) : -294.072 Transmembrane helix 12 from 258 (out) to 273 (in) : -377.138 Transmembrane helix 13 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 14 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 15 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 16 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 277 (in) to 311 (in) : 2450 Re-entrant helix 2 from 312 (in) to 320 (in) : 630 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -348.061 Transmembrane helix 2 from 56 (in) to 71 (out) : -431.165 Transmembrane helix 3 from 75 (out) to 90 (in) : -161.101 Transmembrane helix 4 from 94 (in) to 109 (out) : -220.133 Transmembrane helix 5 from 113 (out) to 128 (in) : 58.816 Transmembrane helix 6 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 7 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 8 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 9 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 10 from 239 (in) to 254 (out) : -294.072 Transmembrane helix 11 from 258 (out) to 273 (in) : -377.138 Transmembrane helix 12 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 13 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 14 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 15 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 277 (in) to 311 (in) : 2450 Re-entrant helix 2 from 312 (in) to 320 (in) : 630 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -343.177 Transmembrane helix 2 from 56 (out) to 71 (in) : -439.835 Transmembrane helix 3 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 4 from 94 (out) to 109 (in) : -220.267 Transmembrane helix 5 from 113 (in) to 128 (out) : 65.884 Transmembrane helix 6 from 140 (out) to 155 (in) : -215.401 Transmembrane helix 7 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 8 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 9 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 10 from 248 (out) to 263 (in) : -183.812 Transmembrane helix 11 from 324 (in) to 339 (out) : -432.694 Transmembrane helix 12 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 13 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 14 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (out) to 244 (out) : 560 Re-entrant helix 2 from 267 (in) to 301 (in) : 2450 Re-entrant helix 3 from 302 (in) to 320 (in) : 1330 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -348.061 Transmembrane helix 2 from 56 (in) to 71 (out) : -431.165 Transmembrane helix 3 from 75 (out) to 90 (in) : -161.101 Transmembrane helix 4 from 94 (in) to 109 (out) : -220.133 Transmembrane helix 5 from 113 (out) to 128 (in) : 58.816 Transmembrane helix 6 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 7 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 8 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 9 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 10 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 11 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 12 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 13 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 335 (out) : 2450 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 35 (in) to 50 (out) : -343.177 Transmembrane helix 2 from 74 (out) to 89 (in) : -159.998 Transmembrane helix 3 from 93 (in) to 108 (out) : -233.478 Transmembrane helix 4 from 112 (out) to 127 (in) : 79.94 Transmembrane helix 5 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 6 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 7 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 8 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 9 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 10 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 11 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 12 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 335 (out) : 2450 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 74 (out) to 89 (in) : -159.998 Transmembrane helix 2 from 93 (in) to 108 (out) : -233.478 Transmembrane helix 3 from 112 (out) to 127 (in) : 79.94 Transmembrane helix 4 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 5 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 6 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 7 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 8 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 9 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 10 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 11 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 335 (out) : 2450 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 141 (in) to 156 (out) : -208.107 Transmembrane helix 4 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 5 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 6 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 7 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 8 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 9 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 10 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 335 (out) : 2450 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 74 (out) to 89 (in) : -159.998 Transmembrane helix 2 from 109 (in) to 124 (out) : 106.596 Transmembrane helix 3 from 167 (out) to 182 (in) : -89.283 Transmembrane helix 4 from 186 (in) to 201 (out) : -135.369 Transmembrane helix 5 from 205 (out) to 220 (in) : -156.506 Transmembrane helix 6 from 247 (in) to 262 (out) : -202.024 Transmembrane helix 7 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 8 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 9 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 224 (in) to 243 (in) : 490 Re-entrant helix 2 from 266 (out) to 300 (out) : 2450 Re-entrant helix 3 from 301 (out) to 335 (out) : 2450 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 4 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 5 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 6 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 7 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 8 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 167 (in) to 182 (out) : -82.717 Transmembrane helix 3 from 186 (out) to 201 (in) : -145.531 Transmembrane helix 4 from 205 (in) to 220 (out) : -175.394 Transmembrane helix 5 from 346 (out) to 361 (in) : -108.044 Transmembrane helix 6 from 365 (in) to 380 (out) : -116.77 Transmembrane helix 7 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 75 (in) to 90 (out) : -149.899 Transmembrane helix 2 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 3 from 169 (in) to 184 (out) : -80.068 Transmembrane helix 4 from 201 (out) to 216 (in) : -139.219 Transmembrane helix 5 from 354 (in) to 369 (out) : -39.231 Transmembrane helix 6 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Re-entrant helix 4 from 342 (in) to 350 (in) : 630 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 169 (in) to 184 (out) : -80.068 Transmembrane helix 3 from 201 (out) to 216 (in) : -139.219 Transmembrane helix 4 from 354 (in) to 369 (out) : -39.231 Transmembrane helix 5 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Re-entrant helix 4 from 342 (in) to 350 (in) : 630 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 109 (in) to 124 (out) : 106.596 Transmembrane helix 2 from 169 (out) to 184 (in) : -74.232 Transmembrane helix 3 from 354 (in) to 369 (out) : -39.231 Transmembrane helix 4 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (in) to 271 (in) : 2450 Re-entrant helix 2 from 272 (in) to 306 (in) : 2450 Re-entrant helix 3 from 307 (in) to 341 (in) : 2450 Re-entrant helix 4 from 342 (in) to 350 (in) : 630 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 109 (out) to 124 (in) : 94.504 Transmembrane helix 2 from 168 (in) to 183 (out) : -78.179 Transmembrane helix 3 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Re-entrant helix 4 from 342 (out) to 376 (out) : 2450 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 109 (in) to 124 (out) : 106.596 Transmembrane helix 2 from 384 (out) to 399 (in) : 28.473 Re-entrant helix 1 from 237 (out) to 271 (out) : 2450 Re-entrant helix 2 from 272 (out) to 306 (out) : 2450 Re-entrant helix 3 from 307 (out) to 341 (out) : 2450 Re-entrant helix 4 from 342 (out) to 376 (out) : 2450 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.106596 1 helix (-) : Score = 0.094504 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 Results: Signal peptide: 1-18 Signal score: 48.93 Topology: 109-124 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.106596