Processing 1 helix: Transmembrane helix 1 from 247 (in) to 262 (out) : 183.782 Score = 0.183782 Processing 1 helix: Transmembrane helix 1 from 247 (out) to 262 (in) : 191.818 Score = 0.191818 Processing 19 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 99 (out) to 114 (in) : -1214.43 Transmembrane helix 7 from 118 (in) to 133 (out) : -777.739 Transmembrane helix 8 from 137 (out) to 152 (in) : -654.435 Transmembrane helix 9 from 161 (in) to 176 (out) : -1093.58 Transmembrane helix 10 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 11 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 12 from 220 (out) to 235 (in) : -705.59 Transmembrane helix 13 from 239 (in) to 254 (out) : -90.274 Transmembrane helix 14 from 258 (out) to 273 (in) : -100.026 Transmembrane helix 15 from 277 (in) to 292 (out) : -890.105 Transmembrane helix 16 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 17 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 18 from 337 (out) to 352 (in) : -454.961 Transmembrane helix 19 from 356 (in) to 371 (out) : -992.983 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 117 (in) to 132 (out) : -839.847 Transmembrane helix 7 from 136 (out) to 151 (in) : -580.416 Transmembrane helix 8 from 161 (in) to 176 (out) : -1093.58 Transmembrane helix 9 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 10 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 11 from 220 (out) to 235 (in) : -705.59 Transmembrane helix 12 from 239 (in) to 254 (out) : -90.274 Transmembrane helix 13 from 258 (out) to 273 (in) : -100.026 Transmembrane helix 14 from 277 (in) to 292 (out) : -890.105 Transmembrane helix 15 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 16 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 17 from 337 (out) to 352 (in) : -454.961 Transmembrane helix 18 from 356 (in) to 371 (out) : -992.983 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 7 from 161 (in) to 176 (out) : -1093.58 Transmembrane helix 8 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 9 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 10 from 220 (out) to 235 (in) : -705.59 Transmembrane helix 11 from 239 (in) to 254 (out) : -90.274 Transmembrane helix 12 from 258 (out) to 273 (in) : -100.026 Transmembrane helix 13 from 277 (in) to 292 (out) : -890.105 Transmembrane helix 14 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 15 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 16 from 337 (out) to 352 (in) : -454.961 Transmembrane helix 17 from 356 (in) to 371 (out) : -992.983 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 7 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 8 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 9 from 220 (out) to 235 (in) : -705.59 Transmembrane helix 10 from 239 (in) to 254 (out) : -90.274 Transmembrane helix 11 from 258 (out) to 273 (in) : -100.026 Transmembrane helix 12 from 277 (in) to 292 (out) : -890.105 Transmembrane helix 13 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 14 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 15 from 337 (out) to 352 (in) : -454.961 Transmembrane helix 16 from 356 (in) to 371 (out) : -992.983 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 7 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 8 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 9 from 220 (in) to 235 (out) : -714.21 Transmembrane helix 10 from 239 (out) to 254 (in) : -101.126 Transmembrane helix 11 from 258 (in) to 273 (out) : -104.074 Transmembrane helix 12 from 277 (out) to 292 (in) : -902.795 Transmembrane helix 13 from 300 (in) to 315 (out) : -726.777 Transmembrane helix 14 from 319 (out) to 334 (in) : -471.518 Transmembrane helix 15 from 338 (in) to 353 (out) : -513.63 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 7 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 8 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 9 from 224 (out) to 239 (in) : -669.276 Transmembrane helix 10 from 243 (in) to 258 (out) : 131.482 Transmembrane helix 11 from 262 (out) to 277 (in) : -305.141 Transmembrane helix 12 from 300 (in) to 315 (out) : -726.777 Transmembrane helix 13 from 319 (out) to 334 (in) : -471.518 Transmembrane helix 14 from 338 (in) to 353 (out) : -513.63 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -279.564 Transmembrane helix 2 from 31 (out) to 46 (in) : -406.192 Transmembrane helix 3 from 50 (in) to 65 (out) : -15.307 Transmembrane helix 4 from 78 (out) to 93 (in) : -711.697 Transmembrane helix 5 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 6 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 7 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 8 from 224 (out) to 239 (in) : -669.276 Transmembrane helix 9 from 243 (in) to 258 (out) : 131.482 Transmembrane helix 10 from 262 (out) to 277 (in) : -305.141 Transmembrane helix 11 from 300 (in) to 315 (out) : -726.777 Transmembrane helix 12 from 319 (out) to 334 (in) : -471.518 Transmembrane helix 13 from 338 (in) to 353 (out) : -513.63 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 74 (in) to 89 (out) : -701.954 Transmembrane helix 5 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 6 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 7 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 8 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 9 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 10 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 11 from 304 (out) to 319 (in) : -723.1 Transmembrane helix 12 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -256.184 Transmembrane helix 2 from 32 (out) to 47 (in) : -421.162 Transmembrane helix 3 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 4 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 5 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 6 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 7 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 8 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 9 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 10 from 304 (out) to 319 (in) : -723.1 Transmembrane helix 11 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 5 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 6 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 7 from 224 (out) to 239 (in) : -669.276 Transmembrane helix 8 from 243 (in) to 258 (out) : 131.482 Transmembrane helix 9 from 262 (out) to 277 (in) : -305.141 Transmembrane helix 10 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -256.184 Transmembrane helix 2 from 32 (out) to 47 (in) : -421.162 Transmembrane helix 3 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 4 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 5 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 6 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 7 from 242 (in) to 257 (out) : 101.206 Transmembrane helix 8 from 261 (out) to 276 (in) : -260.824 Transmembrane helix 9 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 5 from 198 (out) to 213 (in) : -313.976 Transmembrane helix 6 from 242 (in) to 257 (out) : 101.206 Transmembrane helix 7 from 261 (out) to 276 (in) : -260.824 Transmembrane helix 8 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -213.588 Transmembrane helix 2 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 3 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 4 from 198 (out) to 213 (in) : -313.976 Transmembrane helix 5 from 242 (in) to 257 (out) : 101.206 Transmembrane helix 6 from 261 (out) to 276 (in) : -260.824 Transmembrane helix 7 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -216.212 Transmembrane helix 2 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 3 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 4 from 198 (in) to 213 (out) : -313.824 Transmembrane helix 5 from 247 (out) to 262 (in) : 191.818 Transmembrane helix 6 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -213.588 Transmembrane helix 2 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 3 from 198 (in) to 213 (out) : -313.824 Transmembrane helix 4 from 247 (out) to 262 (in) : 191.818 Transmembrane helix 5 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -216.212 Transmembrane helix 2 from 50 (in) to 65 (out) : -15.307 Transmembrane helix 3 from 247 (out) to 262 (in) : 191.818 Transmembrane helix 4 from 328 (in) to 343 (out) : -204.783 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -213.588 Transmembrane helix 2 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 3 from 247 (in) to 262 (out) : 183.782 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 2 from 247 (in) to 262 (out) : 183.782 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 99 (in) to 114 (out) : -1210.97 Transmembrane helix 7 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 8 from 146 (in) to 161 (out) : -1102.54 Transmembrane helix 9 from 165 (out) to 180 (in) : -1029.42 Transmembrane helix 10 from 184 (in) to 199 (out) : -590.121 Transmembrane helix 11 from 203 (out) to 218 (in) : -398.331 Transmembrane helix 12 from 222 (in) to 237 (out) : -704.15 Transmembrane helix 13 from 241 (out) to 256 (in) : 41.057 Transmembrane helix 14 from 260 (in) to 275 (out) : -220.057 Transmembrane helix 15 from 279 (out) to 294 (in) : -965.109 Transmembrane helix 16 from 299 (in) to 314 (out) : -742.429 Transmembrane helix 17 from 318 (out) to 333 (in) : -519.016 Transmembrane helix 18 from 337 (in) to 352 (out) : -453.239 Transmembrane helix 19 from 356 (out) to 371 (in) : -1004.02 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 7 from 146 (in) to 161 (out) : -1102.54 Transmembrane helix 8 from 165 (out) to 180 (in) : -1029.42 Transmembrane helix 9 from 184 (in) to 199 (out) : -590.121 Transmembrane helix 10 from 203 (out) to 218 (in) : -398.331 Transmembrane helix 11 from 222 (in) to 237 (out) : -704.15 Transmembrane helix 12 from 241 (out) to 256 (in) : 41.057 Transmembrane helix 13 from 260 (in) to 275 (out) : -220.057 Transmembrane helix 14 from 279 (out) to 294 (in) : -965.109 Transmembrane helix 15 from 299 (in) to 314 (out) : -742.429 Transmembrane helix 16 from 318 (out) to 333 (in) : -519.016 Transmembrane helix 17 from 337 (in) to 352 (out) : -453.239 Transmembrane helix 18 from 356 (out) to 371 (in) : -1004.02 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 117 (in) to 132 (out) : -839.847 Transmembrane helix 7 from 136 (out) to 151 (in) : -580.416 Transmembrane helix 8 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 9 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 10 from 220 (in) to 235 (out) : -714.21 Transmembrane helix 11 from 239 (out) to 254 (in) : -101.126 Transmembrane helix 12 from 258 (in) to 273 (out) : -104.074 Transmembrane helix 13 from 277 (out) to 292 (in) : -902.795 Transmembrane helix 14 from 299 (in) to 314 (out) : -742.429 Transmembrane helix 15 from 318 (out) to 333 (in) : -519.016 Transmembrane helix 16 from 337 (in) to 352 (out) : -453.239 Transmembrane helix 17 from 356 (out) to 371 (in) : -1004.02 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 7 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 8 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 9 from 220 (in) to 235 (out) : -714.21 Transmembrane helix 10 from 239 (out) to 254 (in) : -101.126 Transmembrane helix 11 from 258 (in) to 273 (out) : -104.074 Transmembrane helix 12 from 277 (out) to 292 (in) : -902.795 Transmembrane helix 13 from 299 (in) to 314 (out) : -742.429 Transmembrane helix 14 from 318 (out) to 333 (in) : -519.016 Transmembrane helix 15 from 337 (in) to 352 (out) : -453.239 Transmembrane helix 16 from 356 (out) to 371 (in) : -1004.02 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -550.155 Transmembrane helix 2 from 23 (in) to 38 (out) : -240.505 Transmembrane helix 3 from 42 (out) to 57 (in) : -194.955 Transmembrane helix 4 from 61 (in) to 76 (out) : -577.523 Transmembrane helix 5 from 80 (out) to 95 (in) : -745.859 Transmembrane helix 6 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 7 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 8 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 9 from 220 (out) to 235 (in) : -705.59 Transmembrane helix 10 from 239 (in) to 254 (out) : -90.274 Transmembrane helix 11 from 258 (out) to 273 (in) : -100.026 Transmembrane helix 12 from 277 (in) to 292 (out) : -890.105 Transmembrane helix 13 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 14 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 15 from 337 (out) to 352 (in) : -454.961 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -549.145 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.895 Transmembrane helix 3 from 42 (in) to 57 (out) : -192.145 Transmembrane helix 4 from 61 (out) to 76 (in) : -570.077 Transmembrane helix 5 from 80 (in) to 95 (out) : -743.541 Transmembrane helix 6 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 7 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 8 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 9 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 10 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 11 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 12 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 13 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 14 from 337 (out) to 352 (in) : -454.961 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 74 (in) to 89 (out) : -701.954 Transmembrane helix 5 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 6 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 7 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 8 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 9 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 10 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 11 from 299 (out) to 314 (in) : -749.271 Transmembrane helix 12 from 318 (in) to 333 (out) : -522.384 Transmembrane helix 13 from 337 (out) to 352 (in) : -454.961 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -279.564 Transmembrane helix 2 from 31 (out) to 46 (in) : -406.192 Transmembrane helix 3 from 50 (in) to 65 (out) : -15.307 Transmembrane helix 4 from 78 (out) to 93 (in) : -711.697 Transmembrane helix 5 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 6 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 7 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 8 from 224 (out) to 239 (in) : -669.276 Transmembrane helix 9 from 243 (in) to 258 (out) : 131.482 Transmembrane helix 10 from 262 (out) to 277 (in) : -305.141 Transmembrane helix 11 from 304 (in) to 319 (out) : -721 Transmembrane helix 12 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 74 (in) to 89 (out) : -701.954 Transmembrane helix 5 from 128 (out) to 143 (in) : -309.67 Transmembrane helix 6 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 7 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 8 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 9 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 10 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 11 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -256.184 Transmembrane helix 2 from 32 (out) to 47 (in) : -421.162 Transmembrane helix 3 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 4 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 5 from 180 (in) to 195 (out) : -602.447 Transmembrane helix 6 from 199 (out) to 214 (in) : -321.366 Transmembrane helix 7 from 224 (in) to 239 (out) : -670.624 Transmembrane helix 8 from 243 (out) to 258 (in) : 132.818 Transmembrane helix 9 from 262 (in) to 277 (out) : -321.259 Transmembrane helix 10 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -286.136 Transmembrane helix 2 from 31 (in) to 46 (out) : -385.908 Transmembrane helix 3 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 4 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 5 from 180 (out) to 195 (in) : -595.753 Transmembrane helix 6 from 199 (in) to 214 (out) : -321.934 Transmembrane helix 7 from 242 (out) to 257 (in) : 99.094 Transmembrane helix 8 from 261 (in) to 276 (out) : -273.176 Transmembrane helix 9 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -256.184 Transmembrane helix 2 from 32 (out) to 47 (in) : -421.162 Transmembrane helix 3 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 4 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 5 from 198 (in) to 213 (out) : -313.824 Transmembrane helix 6 from 242 (out) to 257 (in) : 99.094 Transmembrane helix 7 from 261 (in) to 276 (out) : -273.176 Transmembrane helix 8 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -216.212 Transmembrane helix 2 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 3 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 4 from 198 (in) to 213 (out) : -313.824 Transmembrane helix 5 from 242 (out) to 257 (in) : 99.094 Transmembrane helix 6 from 261 (in) to 276 (out) : -273.176 Transmembrane helix 7 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -213.588 Transmembrane helix 2 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 3 from 128 (in) to 143 (out) : -306.83 Transmembrane helix 4 from 198 (out) to 213 (in) : -313.976 Transmembrane helix 5 from 247 (in) to 262 (out) : 183.782 Transmembrane helix 6 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -216.212 Transmembrane helix 2 from 51 (in) to 66 (out) : -24.713 Transmembrane helix 3 from 127 (out) to 142 (in) : -316.582 Transmembrane helix 4 from 247 (in) to 262 (out) : 183.782 Transmembrane helix 5 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -213.588 Transmembrane helix 2 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 3 from 247 (in) to 262 (out) : 183.782 Transmembrane helix 4 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 50 (out) to 65 (in) : -13.893 Transmembrane helix 2 from 247 (in) to 262 (out) : 183.782 Transmembrane helix 3 from 328 (out) to 343 (in) : -190.317 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -15.307 Transmembrane helix 2 from 247 (out) to 262 (in) : 191.818 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.183782 1 helix (-) : Score = 0.191818 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 247-262 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.191818