Processing 1 helix: Transmembrane helix 1 from 156 (in) to 171 (out) : 662.605 Score = 0.662605 Processing 1 helix: Transmembrane helix 1 from 156 (out) to 171 (in) : 666.195 Score = 0.666195 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -582.022 Transmembrane helix 2 from 23 (out) to 38 (in) : -472.956 Transmembrane helix 3 from 42 (in) to 57 (out) : -781.065 Transmembrane helix 4 from 61 (out) to 76 (in) : -836.068 Transmembrane helix 5 from 85 (in) to 100 (out) : -174.515 Transmembrane helix 6 from 104 (out) to 119 (in) : -582.404 Transmembrane helix 7 from 123 (in) to 138 (out) : -914.726 Transmembrane helix 8 from 147 (out) to 162 (in) : -253.631 Transmembrane helix 9 from 166 (in) to 181 (out) : 224.177 Transmembrane helix 10 from 185 (out) to 200 (in) : -112.31 Transmembrane helix 11 from 204 (in) to 219 (out) : -191.956 Transmembrane helix 12 from 223 (out) to 238 (in) : -440.496 Transmembrane helix 13 from 242 (in) to 257 (out) : -452.394 Transmembrane helix 14 from 261 (out) to 276 (in) : -475.289 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -581.478 Transmembrane helix 2 from 23 (in) to 38 (out) : -462.444 Transmembrane helix 3 from 44 (out) to 59 (in) : -749.682 Transmembrane helix 4 from 63 (in) to 78 (out) : -884.418 Transmembrane helix 5 from 84 (out) to 99 (in) : -211.607 Transmembrane helix 6 from 103 (in) to 118 (out) : -573.255 Transmembrane helix 7 from 122 (out) to 137 (in) : -892.192 Transmembrane helix 8 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 9 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 10 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 11 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 12 from 239 (in) to 254 (out) : -525.564 Transmembrane helix 13 from 258 (out) to 273 (in) : -381.25 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -582.022 Transmembrane helix 2 from 23 (out) to 38 (in) : -472.956 Transmembrane helix 3 from 42 (in) to 57 (out) : -781.065 Transmembrane helix 4 from 61 (out) to 76 (in) : -836.068 Transmembrane helix 5 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 6 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 7 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 8 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 9 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 10 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 11 from 239 (in) to 254 (out) : -525.564 Transmembrane helix 12 from 258 (out) to 273 (in) : -381.25 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -581.478 Transmembrane helix 2 from 23 (in) to 38 (out) : -462.444 Transmembrane helix 3 from 46 (out) to 61 (in) : -737.639 Transmembrane helix 4 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 5 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 6 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 7 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 8 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 9 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 10 from 239 (in) to 254 (out) : -525.564 Transmembrane helix 11 from 258 (out) to 273 (in) : -381.25 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -312.676 Transmembrane helix 2 from 46 (out) to 61 (in) : -737.639 Transmembrane helix 3 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 4 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 5 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 6 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 7 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 8 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 9 from 239 (in) to 254 (out) : -525.564 Transmembrane helix 10 from 258 (out) to 273 (in) : -381.25 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -323.524 Transmembrane helix 2 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 3 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 4 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 5 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 6 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 7 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 8 from 239 (in) to 254 (out) : -525.564 Transmembrane helix 9 from 258 (out) to 273 (in) : -381.25 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -312.676 Transmembrane helix 2 from 86 (out) to 101 (in) : -155.74 Transmembrane helix 3 from 105 (in) to 120 (out) : -595.261 Transmembrane helix 4 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 5 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 6 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 7 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 8 from 250 (out) to 265 (in) : -249.947 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -323.524 Transmembrane helix 2 from 87 (in) to 102 (out) : -148.604 Transmembrane helix 3 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 4 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 5 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 6 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 7 from 250 (out) to 265 (in) : -249.947 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 87 (in) to 102 (out) : -148.604 Transmembrane helix 2 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 3 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 4 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 5 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 6 from 250 (out) to 265 (in) : -249.947 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 87 (out) to 102 (in) : -154.996 Transmembrane helix 2 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 3 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 4 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 5 from 213 (out) to 228 (in) : -190.696 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 87 (in) to 102 (out) : -148.604 Transmembrane helix 2 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 3 from 184 (in) to 199 (out) : -104.828 Transmembrane helix 4 from 206 (out) to 221 (in) : -161.173 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 2 from 184 (in) to 199 (out) : -104.828 Transmembrane helix 3 from 206 (out) to 221 (in) : -161.173 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 2 from 184 (out) to 199 (in) : -106.272 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -581.478 Transmembrane helix 2 from 23 (in) to 38 (out) : -462.444 Transmembrane helix 3 from 44 (out) to 59 (in) : -749.682 Transmembrane helix 4 from 63 (in) to 78 (out) : -884.418 Transmembrane helix 5 from 84 (out) to 99 (in) : -211.607 Transmembrane helix 6 from 103 (in) to 118 (out) : -573.255 Transmembrane helix 7 from 122 (out) to 137 (in) : -892.192 Transmembrane helix 8 from 147 (in) to 162 (out) : -257.069 Transmembrane helix 9 from 166 (out) to 181 (in) : 223.223 Transmembrane helix 10 from 185 (in) to 200 (out) : -109.99 Transmembrane helix 11 from 204 (out) to 219 (in) : -171.844 Transmembrane helix 12 from 223 (in) to 238 (out) : -431.604 Transmembrane helix 13 from 242 (out) to 257 (in) : -444.006 Transmembrane helix 14 from 261 (in) to 276 (out) : -467.911 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -582.022 Transmembrane helix 2 from 23 (out) to 38 (in) : -472.956 Transmembrane helix 3 from 42 (in) to 57 (out) : -781.065 Transmembrane helix 4 from 61 (out) to 76 (in) : -836.068 Transmembrane helix 5 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 6 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 7 from 147 (in) to 162 (out) : -257.069 Transmembrane helix 8 from 166 (out) to 181 (in) : 223.223 Transmembrane helix 9 from 185 (in) to 200 (out) : -109.99 Transmembrane helix 10 from 204 (out) to 219 (in) : -171.844 Transmembrane helix 11 from 223 (in) to 238 (out) : -431.604 Transmembrane helix 12 from 242 (out) to 257 (in) : -444.006 Transmembrane helix 13 from 261 (in) to 276 (out) : -467.911 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -581.478 Transmembrane helix 2 from 23 (in) to 38 (out) : -462.444 Transmembrane helix 3 from 46 (out) to 61 (in) : -737.639 Transmembrane helix 4 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 5 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 6 from 147 (in) to 162 (out) : -257.069 Transmembrane helix 7 from 166 (out) to 181 (in) : 223.223 Transmembrane helix 8 from 185 (in) to 200 (out) : -109.99 Transmembrane helix 9 from 204 (out) to 219 (in) : -171.844 Transmembrane helix 10 from 223 (in) to 238 (out) : -431.604 Transmembrane helix 11 from 242 (out) to 257 (in) : -444.006 Transmembrane helix 12 from 261 (in) to 276 (out) : -467.911 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -582.022 Transmembrane helix 2 from 23 (out) to 38 (in) : -472.956 Transmembrane helix 3 from 45 (in) to 60 (out) : -733.247 Transmembrane helix 4 from 86 (out) to 101 (in) : -155.74 Transmembrane helix 5 from 105 (in) to 120 (out) : -595.261 Transmembrane helix 6 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 7 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 8 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 9 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 10 from 239 (out) to 254 (in) : -512.736 Transmembrane helix 11 from 258 (in) to 273 (out) : -372.55 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -581.478 Transmembrane helix 2 from 23 (in) to 38 (out) : -462.444 Transmembrane helix 3 from 86 (out) to 101 (in) : -155.74 Transmembrane helix 4 from 105 (in) to 120 (out) : -595.261 Transmembrane helix 5 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 6 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 7 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 8 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 9 from 239 (out) to 254 (in) : -512.736 Transmembrane helix 10 from 258 (in) to 273 (out) : -372.55 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -312.676 Transmembrane helix 2 from 86 (out) to 101 (in) : -155.74 Transmembrane helix 3 from 105 (in) to 120 (out) : -595.261 Transmembrane helix 4 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 5 from 175 (in) to 190 (out) : -166.478 Transmembrane helix 6 from 194 (out) to 209 (in) : -177.819 Transmembrane helix 7 from 213 (in) to 228 (out) : -192.504 Transmembrane helix 8 from 239 (out) to 254 (in) : -512.736 Transmembrane helix 9 from 258 (in) to 273 (out) : -372.55 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -323.524 Transmembrane helix 2 from 86 (in) to 101 (out) : -150.86 Transmembrane helix 3 from 105 (out) to 120 (in) : -592.539 Transmembrane helix 4 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 5 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 6 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 7 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 8 from 251 (in) to 266 (out) : -246.05 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -312.676 Transmembrane helix 2 from 86 (out) to 101 (in) : -155.74 Transmembrane helix 3 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 4 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 5 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 6 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 7 from 251 (in) to 266 (out) : -246.05 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 87 (out) to 102 (in) : -154.996 Transmembrane helix 2 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 3 from 175 (out) to 190 (in) : -171.022 Transmembrane helix 4 from 194 (in) to 209 (out) : -174.081 Transmembrane helix 5 from 213 (out) to 228 (in) : -190.696 Transmembrane helix 6 from 251 (in) to 266 (out) : -246.05 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 87 (in) to 102 (out) : -148.604 Transmembrane helix 2 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 3 from 184 (in) to 199 (out) : -104.828 Transmembrane helix 4 from 206 (out) to 221 (in) : -161.173 Transmembrane helix 5 from 251 (in) to 266 (out) : -246.05 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 87 (out) to 102 (in) : -154.996 Transmembrane helix 2 from 156 (in) to 171 (out) : 662.605 Transmembrane helix 3 from 184 (out) to 199 (in) : -106.272 Transmembrane helix 4 from 205 (in) to 220 (out) : -189.368 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 87 (in) to 102 (out) : -148.604 Transmembrane helix 2 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 3 from 184 (in) to 199 (out) : -104.828 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 156 (out) to 171 (in) : 666.195 Transmembrane helix 2 from 184 (in) to 199 (out) : -104.828 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.662605 1 helix (-) : Score = 0.666195 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 156-171 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.666195