BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (67 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q2NWN7 Protein aaeX n=75 Tax=Enterobacteriaceae RepID=A...   104   8e-22
UniRef50_UPI0001A4504C hypothetical protein PcarcW_04569 n=1 Tax...    76   3e-13
UniRef50_Q2KZU0 Putative component of multidrug efflux system n=...    47   2e-04
UniRef50_D1T3C0 Putative uncharacterized protein n=1 Tax=Acidovo...    46   3e-04
UniRef50_A6V0L3 Conserved domain protein n=11 Tax=Proteobacteria...    45   7e-04
UniRef50_B6JAE0 Conserved domain protein n=2 Tax=Bacteria RepID=...    43   0.004
UniRef50_Q2SVA7 Membrane protein, putative n=49 Tax=Burkholderia...    38   0.091
UniRef50_C5CYW8 Putative uncharacterized protein n=1 Tax=Variovo...    38   0.096

>UniRef50_Q2NWN7 Protein aaeX n=75 Tax=Enterobacteriaceae RepID=AAEX_SODGM
          Length = 113

 Score =  104 bits (260), Expect = 8e-22,   Method: Compositional matrix adjust.
 Identities = 48/63 (76%), Positives = 59/63 (93%)

Query: 1  MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFY 60
          MSL PV+V+FGLSFPP+ FE++LSLA+F+ +RR L+P+GIYDFVWHPALFNTALYCC+FY
Sbjct: 1  MSLLPVMVIFGLSFPPVLFEMILSLALFFALRRFLLPSGIYDFVWHPALFNTALYCCVFY 60

Query: 61 LIS 63
          LIS
Sbjct: 61 LIS 63


>UniRef50_UPI0001A4504C hypothetical protein PcarcW_04569 n=1 Tax=Pectobacterium
          carotovorum subsp. carotovorum WPP14
          RepID=UPI0001A4504C
          Length = 98

 Score = 76.3 bits (186), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 35/49 (71%), Positives = 44/49 (89%)

Query: 16 PIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISR 64
          P+FF LL+SL +F++V R+L PTGIYDFVWHPALFN+AL+CCLFYL+ R
Sbjct: 47 PVFFVLLVSLTLFFVVNRLLQPTGIYDFVWHPALFNSALFCCLFYLLFR 95


>UniRef50_Q2KZU0 Putative component of multidrug efflux system n=9
          Tax=Proteobacteria RepID=Q2KZU0_BORA1
          Length = 70

 Score = 47.4 bits (111), Expect = 2e-04,   Method: Compositional matrix adjust.
 Identities = 23/51 (45%), Positives = 33/51 (64%)

Query: 10 FGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFY 60
          +G+ FP +    L +L + W++RR+L   G+Y FVWHPALF+ AL   L Y
Sbjct: 8  YGVYFPWLLVLALPALGLSWMLRRLLARAGLYRFVWHPALFDLALTVVLLY 58


>UniRef50_D1T3C0 Putative uncharacterized protein n=1 Tax=Acidovorax avenae subsp.
          avenae ATCC 19860 RepID=D1T3C0_9BURK
          Length = 72

 Score = 46.2 bits (108), Expect = 3e-04,   Method: Compositional matrix adjust.
 Identities = 21/56 (37%), Positives = 37/56 (66%)

Query: 10 FGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISRL 65
          +G+  P +    +++ A+ W  RR+L   G+Y +VWH ALF+TA+Y  + +L++RL
Sbjct: 8  YGVYVPWLLLLAIVAWALCWGARRLLGSLGVYRWVWHAALFDTAIYVLILFLLTRL 63


>UniRef50_A6V0L3 Conserved domain protein n=11 Tax=Proteobacteria
          RepID=A6V0L3_PSEA7
          Length = 66

 Score = 45.1 bits (105), Expect = 7e-04,   Method: Compositional matrix adjust.
 Identities = 19/57 (33%), Positives = 33/57 (57%)

Query: 7  IVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLIS 63
          I + G+  P +    +++ A+ W + R+    G+Y F WHPALF  +L+ CL+  +S
Sbjct: 5  IAIHGVYMPTLTLLFVIAAALTWGLDRIFASVGLYRFTWHPALFRVSLFACLYGGLS 61


>UniRef50_B6JAE0 Conserved domain protein n=2 Tax=Bacteria RepID=B6JAE0_OLICO
          Length = 65

 Score = 42.7 bits (99), Expect = 0.004,   Method: Compositional matrix adjust.
 Identities = 22/56 (39%), Positives = 33/56 (58%)

Query: 7  IVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLI 62
          I + G+  PP+      ++ I+ ++R  L  T +Y F+WHP LFNTALY  L  +I
Sbjct: 5  INIAGVLLPPLMGYAFAAMIIWLVLRFTLARTNLYRFIWHPPLFNTALYVILLSII 60


>UniRef50_Q2SVA7 Membrane protein, putative n=49 Tax=Burkholderia
          RepID=Q2SVA7_BURTA
          Length = 67

 Score = 38.1 bits (87), Expect = 0.091,   Method: Compositional matrix adjust.
 Identities = 15/30 (50%), Positives = 21/30 (70%)

Query: 29 WLVRRVLVPTGIYDFVWHPALFNTALYCCL 58
          WL+ R+L  TG+Y  VWHP+LF  +L  C+
Sbjct: 28 WLIDRLLAYTGLYRVVWHPSLFRASLLVCI 57


>UniRef50_C5CYW8 Putative uncharacterized protein n=1 Tax=Variovorax paradoxus
          S110 RepID=C5CYW8_VARPS
          Length = 70

 Score = 38.1 bits (87), Expect = 0.096,   Method: Compositional matrix adjust.
 Identities = 18/46 (39%), Positives = 25/46 (54%)

Query: 10 FGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALY 55
           G+  P +      +L   W  RR+L   G Y ++WHPALF+ ALY
Sbjct: 7  LGMQLPWLLLLAAGALPCSWATRRLLAIVGAYRWIWHPALFDLALY 52


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q2NWN7 Protein aaeX n=75 Tax=Enterobacteriaceae RepID=A...    78   6e-14
UniRef50_UPI0001A4504C hypothetical protein PcarcW_04569 n=1 Tax...    61   8e-09
UniRef50_A6V0L3 Conserved domain protein n=11 Tax=Proteobacteria...    57   1e-07
UniRef50_D1T3C0 Putative uncharacterized protein n=1 Tax=Acidovo...    54   1e-06
UniRef50_Q2KZU0 Putative component of multidrug efflux system n=...    47   2e-04

Sequences not found previously or not previously below threshold:

UniRef50_Q88NE7 Conserved domain protein n=14 Tax=Proteobacteria...    43   0.002
UniRef50_C5CYW8 Putative uncharacterized protein n=1 Tax=Variovo...    41   0.015
UniRef50_B0RMD9 Putative membrane protein n=11 Tax=Proteobacteri...    39   0.030
UniRef50_Q2SVA7 Membrane protein, putative n=49 Tax=Burkholderia...    39   0.031


CONVERGED!
>UniRef50_Q2NWN7 Protein aaeX n=75 Tax=Enterobacteriaceae RepID=AAEX_SODGM
          Length = 113

 Score = 78.4 bits (192), Expect = 6e-14,   Method: Composition-based stats.
 Identities = 48/63 (76%), Positives = 59/63 (93%)

Query: 1  MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFY 60
          MSL PV+V+FGLSFPP+ FE++LSLA+F+ +RR L+P+GIYDFVWHPALFNTALYCC+FY
Sbjct: 1  MSLLPVMVIFGLSFPPVLFEMILSLALFFALRRFLLPSGIYDFVWHPALFNTALYCCVFY 60

Query: 61 LIS 63
          LIS
Sbjct: 61 LIS 63


>UniRef50_UPI0001A4504C hypothetical protein PcarcW_04569 n=1 Tax=Pectobacterium
          carotovorum subsp. carotovorum WPP14
          RepID=UPI0001A4504C
          Length = 98

 Score = 61.4 bits (148), Expect = 8e-09,   Method: Composition-based stats.
 Identities = 35/49 (71%), Positives = 44/49 (89%)

Query: 16 PIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISR 64
          P+FF LL+SL +F++V R+L PTGIYDFVWHPALFN+AL+CCLFYL+ R
Sbjct: 47 PVFFVLLVSLTLFFVVNRLLQPTGIYDFVWHPALFNSALFCCLFYLLFR 95


>UniRef50_A6V0L3 Conserved domain protein n=11 Tax=Proteobacteria
          RepID=A6V0L3_PSEA7
          Length = 66

 Score = 57.2 bits (137), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 19/57 (33%), Positives = 33/57 (57%)

Query: 7  IVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLIS 63
          I + G+  P +    +++ A+ W + R+    G+Y F WHPALF  +L+ CL+  +S
Sbjct: 5  IAIHGVYMPTLTLLFVIAAALTWGLDRIFASVGLYRFTWHPALFRVSLFACLYGGLS 61


>UniRef50_D1T3C0 Putative uncharacterized protein n=1 Tax=Acidovorax avenae subsp.
          avenae ATCC 19860 RepID=D1T3C0_9BURK
          Length = 72

 Score = 54.1 bits (129), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 21/56 (37%), Positives = 37/56 (66%)

Query: 10 FGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISRL 65
          +G+  P +    +++ A+ W  RR+L   G+Y +VWH ALF+TA+Y  + +L++RL
Sbjct: 8  YGVYVPWLLLLAIVAWALCWGARRLLGSLGVYRWVWHAALFDTAIYVLILFLLTRL 63


>UniRef50_Q2KZU0 Putative component of multidrug efflux system n=9
          Tax=Proteobacteria RepID=Q2KZU0_BORA1
          Length = 70

 Score = 46.8 bits (110), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 23/54 (42%), Positives = 34/54 (62%)

Query: 10 FGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLIS 63
          +G+ FP +    L +L + W++RR+L   G+Y FVWHPALF+ AL   L Y + 
Sbjct: 8  YGVYFPWLLVLALPALGLSWMLRRLLARAGLYRFVWHPALFDLALTVVLLYGVF 61


>UniRef50_Q88NE7 Conserved domain protein n=14 Tax=Proteobacteria
          RepID=Q88NE7_PSEPK
          Length = 66

 Score = 43.3 bits (101), Expect = 0.002,   Method: Composition-based stats.
 Identities = 18/57 (31%), Positives = 28/57 (49%)

Query: 7  IVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLIS 63
          I   G+  P +    L +L + W + R +     Y F WHPAL   +L+ CLF  ++
Sbjct: 5  IAFHGVYMPTMTLMFLFALGLAWGLDRFIASHDGYRFFWHPALLRLSLFVCLFGAMA 61


>UniRef50_C5CYW8 Putative uncharacterized protein n=1 Tax=Variovorax paradoxus
          S110 RepID=C5CYW8_VARPS
          Length = 70

 Score = 40.6 bits (94), Expect = 0.015,   Method: Composition-based stats.
 Identities = 19/55 (34%), Positives = 29/55 (52%)

Query: 11 GLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISRL 65
          G+  P +      +L   W  RR+L   G Y ++WHPALF+ ALY  +   ++ L
Sbjct: 8  GMQLPWLLLLAAGALPCSWATRRLLAIVGAYRWIWHPALFDLALYALVLCTLASL 62


>UniRef50_B0RMD9 Putative membrane protein n=11 Tax=Proteobacteria
          RepID=B0RMD9_XANCB
          Length = 89

 Score = 39.5 bits (91), Expect = 0.030,   Method: Composition-based stats.
 Identities = 19/57 (33%), Positives = 29/57 (50%)

Query: 9  VFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLISRL 65
          ++G+  P +   + L+  +   V  +L   G Y  +WHPALFN ALY  L   +  L
Sbjct: 27 LYGVFVPTLLALMTLAYLLNSAVGALLTRAGAYRHIWHPALFNLALYIALLGGLFSL 83


>UniRef50_Q2SVA7 Membrane protein, putative n=49 Tax=Burkholderia
          RepID=Q2SVA7_BURTA
          Length = 67

 Score = 39.5 bits (91), Expect = 0.031,   Method: Composition-based stats.
 Identities = 17/57 (29%), Positives = 29/57 (50%)

Query: 7  IVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFVWHPALFNTALYCCLFYLIS 63
          + +     P +    ++     WL+ R+L  TG+Y  VWHP+LF  +L  C+   +S
Sbjct: 6  LAILDAYVPAVVLMFVIGAFATWLIDRLLAYTGLYRVVWHPSLFRASLLVCICGGLS 62


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.331    0.154    0.503 

Lambda     K      H
   0.267   0.0472    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 308,980,104
Number of Sequences: 3077464
Number of extensions: 11503344
Number of successful extensions: 74904
Number of sequences better than 1.0e-01: 54
Number of HSP's better than  0.1 without gapping: 77
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 74823
Number of HSP's gapped (non-prelim): 83
length of query: 67
length of database: 1,040,396,356
effective HSP length: 39
effective length of query: 28
effective length of database: 920,375,260
effective search space: 25770507280
effective search space used: 25770507280
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.3 bits)
S2: 87 (37.9 bits)